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The map label for this gene is hokD [H]

Identifier: 91210499

GI number: 91210499

Start: 1400045

End: 1400257

Strand: Direct

Name: hokD [H]

Synonym: UTI89_C1474

Alternate gene names: 91210499

Gene position: 1400045-1400257 (Clockwise)

Preceding gene: 91210498

Following gene: 91210500

Centisome position: 27.64

GC content: 51.17

Gene sequence:

>213_bases
ATGAACGGCAAATCTAGGTTAGCCTCTTACGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTT
AATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCC
GAACCGGCCAGACGGAGGTCGCTGTCTTCACAGTTTACGAACCTGAGGAGTAA

Upstream 100 bases:

>100_bases
CTATCTGCCTCAGGCATGAACACCAAAGGCAGATAGAGAAAAGCCCCAGTTAATATTACGCGTCCTGCAAGACGCTTAAC
ATTAATCTGAGGCTCAATCT

Downstream 100 bases:

>100_bases
GAGACCCGGCGAGGGAGAAATCCCTCGCCACCTCTGATGTGTCAGGCATCCTCAACGCACCCGCACGTAACCCGCTTCGG
CGGGTTTTGTTTTTTCCTGG

Product: putative cryptic prophage CP-933M cell killing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 70

Protein sequence:

>70_residues
MNGKSRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTVYEPEE

Sequences:

>Translated_70_residues
MNGKSRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTVYEPEE
>Mature_70_residues
MNGKSRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTVYEPEE

Specific function: When Overexpressed Kill The Cells From The Inside By Interfering With A VItal Function In The Cell Membrane. Overexpression Of Relf Results In Collapse Of The Transmembrane Potential And Concomitant Arrest Of Oxygen Consumption. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hok/gef family [H]

Homologues:

Organism=Escherichia coli, GI1787845, Length=51, Percent_Identity=98.0392156862745, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI1786200, Length=57, Percent_Identity=78.9473684210526, Blast_Score=93, Evalue=3e-21,
Organism=Escherichia coli, GI48994874, Length=50, Percent_Identity=80, Blast_Score=86, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000021
- InterPro:   IPR018084
- ProDom:   PD005979 [H]

Pfam domain/function: PF01848 HOK_GEF [H]

EC number: NA

Molecular weight: Translated: 7840; Mature: 7840

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00556 HOK_GEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKSRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEV
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
AVFTVYEPEE
EEEEEECCCC
>Mature Secondary Structure
MNGKSRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEV
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
AVFTVYEPEE
EEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]