Click here to switch to the map view.

The map label for this gene is tsp [H]

Identifier: 89898626

GI number: 89898626

Start: 963353

End: 965308

Strand: Direct

Name: tsp [H]

Synonym: CF0819

Alternate gene names: 89898626

Gene position: 963353-965308 (Clockwise)

Preceding gene: 89898625

Following gene: 89898627

Centisome position: 82.6

GC content: 38.7

Gene sequence:

>1956_bases
ATGATAAAAATACTACGTCTTTGCGCTCTTGTTCTAACATTCCTTCCTAGTTTCTCTTTTTCTTCTGAATTATTTCGTGA
AGAGGACATAAGGAAAACTGTAGATAAACTCATTGAGTATCATATCGATATTCAGGATATCTCTTCAGACATTTTGATTC
GCTCCTTAATAGGGTATTCTCAATCTTTTGATCCTCATAAAGCTTATCTTACAGAGCAAGAAGTCAGTAATTTTATCCAG
TCTACGGATATCAAAAAACGTCTATTGAGAAATTACAAAACGAATAATTTTTCCGTTTATCAGAATTTAAATCATGTAGT
TAAGGACAGTATTACTCGTGCGCGTCAATGGCGTTCGGAATGGTTATCTGATCCTGTGGCTTTAGTTAGAGAAGCTTCCA
CGCATACGCTTATAAAGAAGCCTGGTCAATGGGCACATTCTATTCAGGAGCTGAAGGAAAGACAGCGTTCTTTACTACTT
TCCTATATTTCTGTGTATTTGTCAGATGGTTCTAAAGATAGATATTTAGGTAAAGAAGCTTCTTTAACACGACTTTGTGT
ACGTCAACTTGAGGCATATGAAAACCCTTATCTAGGGATTAATGACTATGGGAAACCGATGTCTCTGGAGGAGGAGTCTC
ACCACTTCCATGTGCGTGTTGTTAAAGCTATGGTGCACAGTTTAGATGCGCACACAACCTACTTTAGCAAAGATGAAGCC
CTTGCTATGCGTATTCAATTAGAAAAGGGCATGTGTGGGATTGGAGTTATCTTAAAAGAAGATATCGATGGGGTTATTAT
AAAAGAGATTATTCCTGGAGGCCCTGCAGATAAAACTAGTGAATTGCATGTGGATGATGTGATTTATCGTGTGGACGGTA
AAAGCATAGAAAATCTCCCATTTAAAGCTGTTTTAGATTGTCTTAGAGGCTCTCAAGGTTCTGAGGTGGTCTTAGATGTG
CATAGTAAGGATCAAAACCGCACAGTTAAATTACGAAGAGAAAAGATTAGTTTGGATGATCGCCGTGTGGATGTCTCCTA
TGAGCCTTACGGAGATGGGATTATTGGCAAGATCACCTTGCACTCTTTCTATGAAGGAGAAAACCAGGTTTCTAGCGAAC
AAGATTTAAAGCGTGCTATTCAAAGTTTACAAGATAAGAATCTCCTAGGACTCGTTTTAGACATTCGAGAGAACACCGGA
GGTTTTCTCTCACAAGCCATTAAAGTGTCTGGGTTATTTATGACCAATGGAGTCGTTGTTGTTTCGCGTTATGCGGACGG
CAGTATTAAACGTTACCGCACAATATCTCCTAAAAAATTCTACGATGGGCCTCTAACTATTCTTGTTTCAAAAAGTTCTG
CATCCGCGGCAGAAATTGTTGCTCAAACATTACAAGATTATGGCGTAGCTATAATCGTTGGTGATGAACAAACTTACGGG
AAGGGGACAATCCAGCATCAAACAATTACAGCAGATTCTGATAAAGAAGGATTTTTTAAAGTTACTGTAGGAAAATACTA
CTCCCCTTCAGGAAAGTCAACCCAACTTCATGGAGTTCGTTCTGATATTCATATAGCTTCACGTTATTTGGAAGAACCCT
TAGGAGAACGGTATTTAGAGCACCCCCTACCTTCGGATAATTGTGATAATGTCATGGATGACAATTTGGGGGATCTTGAT
TCTCATATGCGTCCGTGGTTTCAGAAATACTACCTTCCAAATCTACAGAAGGAAGAGACGGTGTGGAGAGAGATGCTTCC
CCAGCTTTCAGCGAATAGTCAACAGCGATTAAATGAAAATAAAAACTACAAGATCTTTTTGGATGAATTAAAAGGTTCTT
CGGATGAAATGCGTTCTTTTGGTAGCAACGATTTACAGATGGAAGAATCTGTGAATATTTTAAAAGACATGATTCTTCTA
AGAGATCGTAAAGCTTCGGTTTCTTTAGGAGGCTAA

Upstream 100 bases:

>100_bases
TTATTCTGAATAGTTTTTTCTTGAAGTCCAAAATCTTTGCTCAAGCCTGAGCGTTTTGTGCCGATGTTACGAGTATCCAT
GATCAACAAAGATACTTGTA

Downstream 100 bases:

>100_bases
CTGCCTCCTAAAGTCTTTATAAAGTGAAATCCTCTATCCCAACTATGATTTAGAAGACTCCGGATCATTACTATTCATAG
TAAAAATCGTATTCGACTGG

Product: cell division related penicillin-binding proteinase

Products: NA

Alternate protein names: C-terminal-processing peptidase; PRC protein; Protease Re [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MIKILRLCALVLTFLPSFSFSSELFREEDIRKTVDKLIEYHIDIQDISSDILIRSLIGYSQSFDPHKAYLTEQEVSNFIQ
STDIKKRLLRNYKTNNFSVYQNLNHVVKDSITRARQWRSEWLSDPVALVREASTHTLIKKPGQWAHSIQELKERQRSLLL
SYISVYLSDGSKDRYLGKEASLTRLCVRQLEAYENPYLGINDYGKPMSLEEESHHFHVRVVKAMVHSLDAHTTYFSKDEA
LAMRIQLEKGMCGIGVILKEDIDGVIIKEIIPGGPADKTSELHVDDVIYRVDGKSIENLPFKAVLDCLRGSQGSEVVLDV
HSKDQNRTVKLRREKISLDDRRVDVSYEPYGDGIIGKITLHSFYEGENQVSSEQDLKRAIQSLQDKNLLGLVLDIRENTG
GFLSQAIKVSGLFMTNGVVVVSRYADGSIKRYRTISPKKFYDGPLTILVSKSSASAAEIVAQTLQDYGVAIIVGDEQTYG
KGTIQHQTITADSDKEGFFKVTVGKYYSPSGKSTQLHGVRSDIHIASRYLEEPLGERYLEHPLPSDNCDNVMDDNLGDLD
SHMRPWFQKYYLPNLQKEETVWREMLPQLSANSQQRLNENKNYKIFLDELKGSSDEMRSFGSNDLQMEESVNILKDMILL
RDRKASVSLGG

Sequences:

>Translated_651_residues
MIKILRLCALVLTFLPSFSFSSELFREEDIRKTVDKLIEYHIDIQDISSDILIRSLIGYSQSFDPHKAYLTEQEVSNFIQ
STDIKKRLLRNYKTNNFSVYQNLNHVVKDSITRARQWRSEWLSDPVALVREASTHTLIKKPGQWAHSIQELKERQRSLLL
SYISVYLSDGSKDRYLGKEASLTRLCVRQLEAYENPYLGINDYGKPMSLEEESHHFHVRVVKAMVHSLDAHTTYFSKDEA
LAMRIQLEKGMCGIGVILKEDIDGVIIKEIIPGGPADKTSELHVDDVIYRVDGKSIENLPFKAVLDCLRGSQGSEVVLDV
HSKDQNRTVKLRREKISLDDRRVDVSYEPYGDGIIGKITLHSFYEGENQVSSEQDLKRAIQSLQDKNLLGLVLDIRENTG
GFLSQAIKVSGLFMTNGVVVVSRYADGSIKRYRTISPKKFYDGPLTILVSKSSASAAEIVAQTLQDYGVAIIVGDEQTYG
KGTIQHQTITADSDKEGFFKVTVGKYYSPSGKSTQLHGVRSDIHIASRYLEEPLGERYLEHPLPSDNCDNVMDDNLGDLD
SHMRPWFQKYYLPNLQKEETVWREMLPQLSANSQQRLNENKNYKIFLDELKGSSDEMRSFGSNDLQMEESVNILKDMILL
RDRKASVSLGG
>Mature_651_residues
MIKILRLCALVLTFLPSFSFSSELFREEDIRKTVDKLIEYHIDIQDISSDILIRSLIGYSQSFDPHKAYLTEQEVSNFIQ
STDIKKRLLRNYKTNNFSVYQNLNHVVKDSITRARQWRSEWLSDPVALVREASTHTLIKKPGQWAHSIQELKERQRSLLL
SYISVYLSDGSKDRYLGKEASLTRLCVRQLEAYENPYLGINDYGKPMSLEEESHHFHVRVVKAMVHSLDAHTTYFSKDEA
LAMRIQLEKGMCGIGVILKEDIDGVIIKEIIPGGPADKTSELHVDDVIYRVDGKSIENLPFKAVLDCLRGSQGSEVVLDV
HSKDQNRTVKLRREKISLDDRRVDVSYEPYGDGIIGKITLHSFYEGENQVSSEQDLKRAIQSLQDKNLLGLVLDIRENTG
GFLSQAIKVSGLFMTNGVVVVSRYADGSIKRYRTISPKKFYDGPLTILVSKSSASAAEIVAQTLQDYGVAIIVGDEQTYG
KGTIQHQTITADSDKEGFFKVTVGKYYSPSGKSTQLHGVRSDIHIASRYLEEPLGERYLEHPLPSDNCDNVMDDNLGDLD
SHMRPWFQKYYLPNLQKEETVWREMLPQLSANSQQRLNENKNYKIFLDELKGSSDEMRSFGSNDLQMEESVNILKDMILL
RDRKASVSLGG

Specific function: Involved in the cleavage of a C-terminal peptide of 11 residues from the precursor form of penicillin-binding protein 3 (PBP3). May be involved in protection of the bacterium from thermal and osmotic stresses [H]

COG id: COG0793

COG function: function code M; Periplasmic protease

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1788134, Length=357, Percent_Identity=33.0532212885154, Blast_Score=186, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR005151
- InterPro:   IPR004447
- InterPro:   IPR020992 [H]

Pfam domain/function: PF11818 DUF3340; PF00595 PDZ; PF03572 Peptidase_S41 [H]

EC number: =3.4.21.102 [H]

Molecular weight: Translated: 74030; Mature: 74030

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKILRLCALVLTFLPSFSFSSELFREEDIRKTVDKLIEYHIDIQDISSDILIRSLIGYS
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
QSFDPHKAYLTEQEVSNFIQSTDIKKRLLRNYKTNNFSVYQNLNHVVKDSITRARQWRSE
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WLSDPVALVREASTHTLIKKPGQWAHSIQELKERQRSLLLSYISVYLSDGSKDRYLGKEA
HHCCHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
SLTRLCVRQLEAYENPYLGINDYGKPMSLEEESHHFHVRVVKAMVHSLDAHTTYFSKDEA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCE
LAMRIQLEKGMCGIGVILKEDIDGVIIKEIIPGGPADKTSELHVDDVIYRVDGKSIENLP
EEEEEEECCCCCCEEEEEECCCCCEEHHHHCCCCCCCCCCCEEHHHEEEEECCCCCCCCC
FKAVLDCLRGSQGSEVVLDVHSKDQNRTVKLRREKISLDDRRVDVSYEPYGDGIIGKITL
HHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCEEEEEEE
HSFYEGENQVSSEQDLKRAIQSLQDKNLLGLVLDIRENTGGFLSQAIKVSGLFMTNGVVV
HHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCEEEEECCEEE
VSRYADGSIKRYRTISPKKFYDGPLTILVSKSSASAAEIVAQTLQDYGVAIIVGDEQTYG
EEECCCCCCHHEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCC
KGTIQHQTITADSDKEGFFKVTVGKYYSPSGKSTQLHGVRSDIHIASRYLEEPLGERYLE
CCCEEEEEEECCCCCCCEEEEEECCEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
HPLPSDNCDNVMDDNLGDLDSHMRPWFQKYYLPNLQKEETVWREMLPQLSANSQQRLNEN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHCCC
KNYKIFLDELKGSSDEMRSFGSNDLQMEESVNILKDMILLRDRKASVSLGG
CCEEEEEHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIKILRLCALVLTFLPSFSFSSELFREEDIRKTVDKLIEYHIDIQDISSDILIRSLIGYS
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
QSFDPHKAYLTEQEVSNFIQSTDIKKRLLRNYKTNNFSVYQNLNHVVKDSITRARQWRSE
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WLSDPVALVREASTHTLIKKPGQWAHSIQELKERQRSLLLSYISVYLSDGSKDRYLGKEA
HHCCHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
SLTRLCVRQLEAYENPYLGINDYGKPMSLEEESHHFHVRVVKAMVHSLDAHTTYFSKDEA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCE
LAMRIQLEKGMCGIGVILKEDIDGVIIKEIIPGGPADKTSELHVDDVIYRVDGKSIENLP
EEEEEEECCCCCCEEEEEECCCCCEEHHHHCCCCCCCCCCCEEHHHEEEEECCCCCCCCC
FKAVLDCLRGSQGSEVVLDVHSKDQNRTVKLRREKISLDDRRVDVSYEPYGDGIIGKITL
HHHHHHHHCCCCCCEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEECCCCCCEEEEEEE
HSFYEGENQVSSEQDLKRAIQSLQDKNLLGLVLDIRENTGGFLSQAIKVSGLFMTNGVVV
HHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHCEEEEECCEEE
VSRYADGSIKRYRTISPKKFYDGPLTILVSKSSASAAEIVAQTLQDYGVAIIVGDEQTYG
EEECCCCCCHHEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCC
KGTIQHQTITADSDKEGFFKVTVGKYYSPSGKSTQLHGVRSDIHIASRYLEEPLGERYLE
CCCEEEEEEECCCCCCCEEEEEECCEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
HPLPSDNCDNVMDDNLGDLDSHMRPWFQKYYLPNLQKEETVWREMLPQLSANSQQRLNEN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHCCC
KNYKIFLDELKGSSDEMRSFGSNDLQMEESVNILKDMILLRDRKASVSLGG
CCEEEEEHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1856173; 1729701; 9097040; 9278503; 1447154; 10049386; 7499412; 8576225 [H]