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The map label for this gene is ispF [H]

Identifier: 89898619

GI number: 89898619

Start: 957245

End: 957775

Strand: Direct

Name: ispF [H]

Synonym: CF0812

Alternate gene names: 89898619

Gene position: 957245-957775 (Clockwise)

Preceding gene: 89898614

Following gene: 89898625

Centisome position: 82.08

GC content: 42.75

Gene sequence:

>531_bases
ATGAACGCAGAAAAAGACTCTCCCTTGCCTAAACCACAATGGGTTTATCGTGTAGGAATCGGGCAAGACAGCCATCGTTT
CCTCTCTGAAAGTTCTGCAAAACCCTGCATTTTAGCTGGGGTAATCTTTGAAAATAGTCCTGGTTTCCAAGCAAATTCCG
ATGGTGATATTGTTTTCCACGCTATTTGCAACGCCATCTCTTCCGTCACGCATAGAATCATACTAGGAGACGTTGCTGAC
GAGCTTTTCCACACGCGAGGCATTACTGACAGCAGCATATATTTATCTGAAGCTATAAAGTCATTAAAACCGAACCAATT
GATTTCTCACGTAGCTATTACTATTGAGGGAAATCGTCCTAAATTTCTTCCCAAGCTATCTGCTATGCGGCAAAGCATAG
CCTCAGCTTTAAATATATCTTTAGGATCCGTAGGCATTACTGCGACTTCTGGGGAAGGCTTGAGTGATTTTGGTTGTGGC
GATGGCGTACAATGTTTTTGCGTACTTACAGTTATGGAGTATTGCGGTTAA

Upstream 100 bases:

>100_bases
AGATTTTCAACTACAGGAAGTCCCTGGAGATTTGGGAATACGAAAACTTTCTCAAATAGCAGTAAATCTCGATAAAGGAA
TTTAAAGCGGTATAACAAAT

Downstream 100 bases:

>100_bases
TAGACATCGACAACGTAACGACGCTCTTCCTTTAACTGGAAGAGCTGATTTTTCCCTAGGATATCTTCCAGGGTCTTTCT
AACTTCTGTTCCTAGGACTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MNAEKDSPLPKPQWVYRVGIGQDSHRFLSESSAKPCILAGVIFENSPGFQANSDGDIVFHAICNAISSVTHRIILGDVAD
ELFHTRGITDSSIYLSEAIKSLKPNQLISHVAITIEGNRPKFLPKLSAMRQSIASALNISLGSVGITATSGEGLSDFGCG
DGVQCFCVLTVMEYCG

Sequences:

>Translated_176_residues
MNAEKDSPLPKPQWVYRVGIGQDSHRFLSESSAKPCILAGVIFENSPGFQANSDGDIVFHAICNAISSVTHRIILGDVAD
ELFHTRGITDSSIYLSEAIKSLKPNQLISHVAITIEGNRPKFLPKLSAMRQSIASALNISLGSVGITATSGEGLSDFGCG
DGVQCFCVLTVMEYCG
>Mature_176_residues
MNAEKDSPLPKPQWVYRVGIGQDSHRFLSESSAKPCILAGVIFENSPGFQANSDGDIVFHAICNAISSVTHRIILGDVAD
ELFHTRGITDSSIYLSEAIKSLKPNQLISHVAITIEGNRPKFLPKLSAMRQSIASALNISLGSVGITATSGEGLSDFGCG
DGVQCFCVLTVMEYCG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 18789; Mature: 18789

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEKDSPLPKPQWVYRVGIGQDSHRFLSESSAKPCILAGVIFENSPGFQANSDGDIVFH
CCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCHHH
AICNAISSVTHRIILGDVADELFHTRGITDSSIYLSEAIKSLKPNQLISHVAITIEGNRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHEEEEEEEEECCCC
KFLPKLSAMRQSIASALNISLGSVGITATSGEGLSDFGCGDGVQCFCVLTVMEYCG
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAEKDSPLPKPQWVYRVGIGQDSHRFLSESSAKPCILAGVIFENSPGFQANSDGDIVFH
CCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCHHH
AICNAISSVTHRIILGDVADELFHTRGITDSSIYLSEAIKSLKPNQLISHVAITIEGNRP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHEEEEEEEEECCCC
KFLPKLSAMRQSIASALNISLGSVGITATSGEGLSDFGCGDGVQCFCVLTVMEYCG
CCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA