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The map label for this gene is cofH [H]

Identifier: 89898583

GI number: 89898583

Start: 919483

End: 920598

Strand: Direct

Name: cofH [H]

Synonym: CF0776

Alternate gene names: 89898583

Gene position: 919483-920598 (Clockwise)

Preceding gene: 89898582

Following gene: 89898584

Centisome position: 78.84

GC content: 39.7

Gene sequence:

>1116_bases
ATGACAACGACACCCCTTCCTCGCATTCTTCTTAAAAACTCTTGGATGAAACAGTTATTTGATACGTATACGGAAGGAGC
TCGTCTTTCTACAGACGAAGCCTTGCGTCTACTTCTCTTAGAAAATGAAGACGATCAGCGTGCCTTATGGGACTTTGCAG
ACCAAGTACGTAAAAAGTATGTTGGAGATGTTGTCTACTACTCTTCCACCTTTTATTTATATCCCACGAACTTTTGTGAC
TTCAACTGTACCTTCTGTGCTTTTTATGCGAAACCGGGAGATGCTAAAGGATGGTTTTATACTCCTGACCAACTTATCCA
AAAGATTCATGAGTTAGACGTTCCCGTTACAGAAGCACATATCGTTTCAGGATGCTTTCCAGAGTGCAATTTAGACTACT
ATACTGAGCTATTCAGAAAGATTAAGACCAATTTCCCTCATATCCATATTAAAGCACTCACCGGTATTGAGTATGCTTAC
CTAGCGCAACTTCACAACATTCCTGTTGTAGAGGTATTGAAAATCTTAAAAAACTCAGGATTAGATTCCATCCCTGGAGG
AGGTTTTGATATCCTTGTAGATGAAATACGCCAAGTACTGGCTCCGGGACGATTATCCTCTCAAGCTTTCTTAGATATTC
ATAGAACAGCGCATAATCTTGGAATACCAAGCAATAGCACGATGTTGTGCTATCATAGGGAGAAGCCTGAAGATCTCGTC
ACACATATGGAAAAGTTAAGAAATCTCCAAGACGATACATTAGGATTTAAGAACTTTGTGTTGTTGAAGTTCGCAACAGA
AAATAATCCCCTAGGGAAAAGACTTCGCAAAATCGGAAGATTTCATAATATCCCACCGGCATCTATCATTGCTGTGGCAA
GGCTATTCTTAGATAATTTTAGAAACATAAAAGCCTTGTGGAATTATTTAGGAATCGAGCAAGCTCTACACTTGCTATCT
TGCGGAGCGAATGATCTTTCCTCCACACATATCGGAGAAAAGGTGTTTCAAATGGCCTCTTCAAATCAAAGTATAAAAAT
GGATATTGAGGGAATGGCAAGCCTCATTAAAAAACAAGGGCGCATACCATGTCTGACAAACTCGAAAGACGTATAA

Upstream 100 bases:

>100_bases
GGCAGGCCTTTATCTAAAGGATGTGGAAAATCGAACTGTTGCCATAAAAAAGCGTGCGATAAACAAACTCGATCTGATTC
TACAACCAAAGCATCATCCC

Downstream 100 bases:

>100_bases
CTTTGGGATGCGTAAGCTATATCAATGCTTTTCCTTTTTCTTTGGAGCTTGCAAAAAGAGAGGACATTCTTCTTCATACA
GCCCCTCCAGCAAATTTACT

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTTTPLPRILLKNSWMKQLFDTYTEGARLSTDEALRLLLLENEDDQRALWDFADQVRKKYVGDVVYYSSTFYLYPTNFCD
FNCTFCAFYAKPGDAKGWFYTPDQLIQKIHELDVPVTEAHIVSGCFPECNLDYYTELFRKIKTNFPHIHIKALTGIEYAY
LAQLHNIPVVEVLKILKNSGLDSIPGGGFDILVDEIRQVLAPGRLSSQAFLDIHRTAHNLGIPSNSTMLCYHREKPEDLV
THMEKLRNLQDDTLGFKNFVLLKFATENNPLGKRLRKIGRFHNIPPASIIAVARLFLDNFRNIKALWNYLGIEQALHLLS
CGANDLSSTHIGEKVFQMASSNQSIKMDIEGMASLIKKQGRIPCLTNSKDV

Sequences:

>Translated_371_residues
MTTTPLPRILLKNSWMKQLFDTYTEGARLSTDEALRLLLLENEDDQRALWDFADQVRKKYVGDVVYYSSTFYLYPTNFCD
FNCTFCAFYAKPGDAKGWFYTPDQLIQKIHELDVPVTEAHIVSGCFPECNLDYYTELFRKIKTNFPHIHIKALTGIEYAY
LAQLHNIPVVEVLKILKNSGLDSIPGGGFDILVDEIRQVLAPGRLSSQAFLDIHRTAHNLGIPSNSTMLCYHREKPEDLV
THMEKLRNLQDDTLGFKNFVLLKFATENNPLGKRLRKIGRFHNIPPASIIAVARLFLDNFRNIKALWNYLGIEQALHLLS
CGANDLSSTHIGEKVFQMASSNQSIKMDIEGMASLIKKQGRIPCLTNSKDV
>Mature_370_residues
TTTPLPRILLKNSWMKQLFDTYTEGARLSTDEALRLLLLENEDDQRALWDFADQVRKKYVGDVVYYSSTFYLYPTNFCDF
NCTFCAFYAKPGDAKGWFYTPDQLIQKIHELDVPVTEAHIVSGCFPECNLDYYTELFRKIKTNFPHIHIKALTGIEYAYL
AQLHNIPVVEVLKILKNSGLDSIPGGGFDILVDEIRQVLAPGRLSSQAFLDIHRTAHNLGIPSNSTMLCYHREKPEDLVT
HMEKLRNLQDDTLGFKNFVLLKFATENNPLGKRLRKIGRFHNIPPASIIAVARLFLDNFRNIKALWNYLGIEQALHLLSC
GANDLSSTHIGEKVFQMASSNQSIKMDIEGMASLIKKQGRIPCLTNSKDV

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42269; Mature: 42138

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPLPRILLKNSWMKQLFDTYTEGARLSTDEALRLLLLENEDDQRALWDFADQVRKKY
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHH
VGDVVYYSSTFYLYPTNFCDFNCTFCAFYAKPGDAKGWFYTPDQLIQKIHELDVPVTEAH
HCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCEECHHHHHHHHHHCCCCHHHHH
IVSGCFPECNLDYYTELFRKIKTNFPHIHIKALTGIEYAYLAQLHNIPVVEVLKILKNSG
HHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHCC
LDSIPGGGFDILVDEIRQVLAPGRLSSQAFLDIHRTAHNLGIPSNSTMLCYHREKPEDLV
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
THMEKLRNLQDDTLGFKNFVLLKFATENNPLGKRLRKIGRFHNIPPASIIAVARLFLDNF
HHHHHHHCCCHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RNIKALWNYLGIEQALHLLSCGANDLSSTHIGEKVFQMASSNQSIKMDIEGMASLIKKQG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCC
RIPCLTNSKDV
CCCCCCCCCCC
>Mature Secondary Structure 
TTTPLPRILLKNSWMKQLFDTYTEGARLSTDEALRLLLLENEDDQRALWDFADQVRKKY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCCCHHHHHHHHHHHHHHH
VGDVVYYSSTFYLYPTNFCDFNCTFCAFYAKPGDAKGWFYTPDQLIQKIHELDVPVTEAH
HCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCEECHHHHHHHHHHCCCCHHHHH
IVSGCFPECNLDYYTELFRKIKTNFPHIHIKALTGIEYAYLAQLHNIPVVEVLKILKNSG
HHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHCC
LDSIPGGGFDILVDEIRQVLAPGRLSSQAFLDIHRTAHNLGIPSNSTMLCYHREKPEDLV
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
THMEKLRNLQDDTLGFKNFVLLKFATENNPLGKRLRKIGRFHNIPPASIIAVARLFLDNF
HHHHHHHCCCHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RNIKALWNYLGIEQALHLLSCGANDLSSTHIGEKVFQMASSNQSIKMDIEGMASLIKKQG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCC
RIPCLTNSKDV
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA