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The map label for this gene is grpE [H]

Identifier: 89898571

GI number: 89898571

Start: 908618

End: 909181

Strand: Direct

Name: grpE [H]

Synonym: CF0764

Alternate gene names: 89898571

Gene position: 908618-909181 (Clockwise)

Preceding gene: 89898570

Following gene: 89898572

Centisome position: 77.91

GC content: 38.3

Gene sequence:

>564_bases
ATGACAGATTCCTCTAACGAACATGAAACAGAAAATCCATCAGTTCCTAACCCCGATAACGAGATTCAGGATCTCCAACA
AGAAATAGCGACGTTAAAAGCTGAGTTAAAAGAAAAAAATGATAAATACTTAATGGTCCTTGCGGAATCGGAAAACGCTC
GCAAACGCATGCAAAAAGAGCGTCAGGAAATGATGCAGTATGCTGTGGAAAATGCTCTTATAGACTTTTTAGTGCCCATA
GAAAGCATGGAAAAAGCTTTAGGATTTGCCTCTCAAATGTCAGACGAAGTAAAAAACTGGGCGCTAGGATTCAATATGAT
CCTACAACAATTTAAACAGGTTTTTGAAGAAAAAGGTATTGTTGAATACTCCTCTGTAGGACAAAAATTTAATCCATTTT
TACATGAGGCAGTAGAAACAGAGGAAACAACAAAAGTCCCCGAAGGAATTATCGTGGAAGAATTTGCTAAAGGCTACAAA
ATCGGAGACCGTCCTATACGCGTTGCAAAAGTCAAAGTAGCAAAATCTCCCGCGCCCCAAGAGAAAGAAGAAGAAAAAAA
ATAA

Upstream 100 bases:

>100_bases
CCTTCAGAAGACCTTGTCCTTCCGATCCTAAACTCTCTAAAGAACCCACGTTACTGGCAAGATACTCTTCTATAAAACTA
TTACCCCCCAAGGAGACTTC

Downstream 100 bases:

>100_bases
TGAAACCCTAGAGATTAGGTACGAACCATGAGCGAACACAAAAAATCTAGTAAAATTATAGGTATAGACTTAGGAACAAC
AAACTCCTGCGTATCTGTGA

Product: heat shock protein HSP70 cofactor

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTDSSNEHETENPSVPNPDNEIQDLQQEIATLKAELKEKNDKYLMVLAESENARKRMQKERQEMMQYAVENALIDFLVPI
ESMEKALGFASQMSDEVKNWALGFNMILQQFKQVFEEKGIVEYSSVGQKFNPFLHEAVETEETTKVPEGIIVEEFAKGYK
IGDRPIRVAKVKVAKSPAPQEKEEEKK

Sequences:

>Translated_187_residues
MTDSSNEHETENPSVPNPDNEIQDLQQEIATLKAELKEKNDKYLMVLAESENARKRMQKERQEMMQYAVENALIDFLVPI
ESMEKALGFASQMSDEVKNWALGFNMILQQFKQVFEEKGIVEYSSVGQKFNPFLHEAVETEETTKVPEGIIVEEFAKGYK
IGDRPIRVAKVKVAKSPAPQEKEEEKK
>Mature_186_residues
TDSSNEHETENPSVPNPDNEIQDLQQEIATLKAELKEKNDKYLMVLAESENARKRMQKERQEMMQYAVENALIDFLVPIE
SMEKALGFASQMSDEVKNWALGFNMILQQFKQVFEEKGIVEYSSVGQKFNPFLHEAVETEETTKVPEGIIVEEFAKGYKI
GDRPIRVAKVKVAKSPAPQEKEEEKK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=165, Percent_Identity=32.7272727272727, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1788967, Length=156, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24653432, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21442; Mature: 21310

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSNEHETENPSVPNPDNEIQDLQQEIATLKAELKEKNDKYLMVLAESENARKRMQKE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
RQEMMQYAVENALIDFLVPIESMEKALGFASQMSDEVKNWALGFNMILQQFKQVFEEKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEYSSVGQKFNPFLHEAVETEETTKVPEGIIVEEFAKGYKIGDRPIRVAKVKVAKSPAPQ
HHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHEEECCCCCCCC
EKEEEKK
CHHHCCC
>Mature Secondary Structure 
TDSSNEHETENPSVPNPDNEIQDLQQEIATLKAELKEKNDKYLMVLAESENARKRMQKE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
RQEMMQYAVENALIDFLVPIESMEKALGFASQMSDEVKNWALGFNMILQQFKQVFEEKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEYSSVGQKFNPFLHEAVETEETTKVPEGIIVEEFAKGYKIGDRPIRVAKVKVAKSPAPQ
HHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHEEECCCCCCCC
EKEEEKK
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12056482 [H]