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The map label for this gene is dnaA [H]

Identifier: 89898446

GI number: 89898446

Start: 742836

End: 744188

Strand: Direct

Name: dnaA [H]

Synonym: CF0639

Alternate gene names: 89898446

Gene position: 742836-744188 (Clockwise)

Preceding gene: 89898445

Following gene: 89898447

Centisome position: 63.7

GC content: 36.88

Gene sequence:

>1353_bases
ATGTTAACCTGTAACAATTGTAGTACTTGGGAACAGTTTGTGAACTATGTTAAAACACGTTGCTCCAAAACGGCTTTTGA
AAATTGGATTTCTCCTATTCAAATTATAGAAGAAACACAGGAAAAGATCCGTTTAGAAGTTCCCAACATCTTTGTTCAAA
ACTACCTTCTTGATAATTATAAACAAGACCTGTGTTCTTTTGTTCCTCTTGATGCCCAAGGAGAGCCGGCTTTAGAATTT
GTTGTTGCAGAAATCAAAAAATCTCCTGTGCAACCTGTCGCACCTCGAGAACAGAAAGAGAGCCCTGCAGAAACTTTTGA
AGGATCTAAAGACTTTGAACTAAAGTTAAACACAGCCTATCGTTTTGATAATTTCATAGAAGGCCCTTCAAACCAATTTG
TCAAATCTGCAGCTGTAGGTATCGCCGGGCGCCCAGGACGTTCGTACAATCCTCTATTTATTCATGGAGGTGTAGGGTTA
GGGAAAACACATCTGCTCCATGCCGTAGGTCACTACGTTAAAGAACATCACAAAAATCTCCGAGTGCACTGCATTACTAC
AGAAGCTTTTATCAATGATCTCGTACAACATCTACGATTGAAATCTATCGACAAAATGAAAAATTTTTATCGATCTTTGG
ATCTGCTTCTTGTCGACGATATTCAGTTTTTGCAAAATAGACAAAATTTCGAAGAAGAGTTTTGTAATACTTTTGAGACG
TTGATTAATTTAAATAAACAAATCGTCATCACTAGTGATAAACCACCGGGACAATTAAAACTTTCGGAGCGCATTATTGC
TAGAATGGAATGGGGATTGGTTGCTCATGTAGGTATTCCTGATTTAGAAACTCGGGTAGCAATTTTACAGCATAAAGCAG
AACAGAAAGGCTTACATATTCCTAATGAAATTGCTTTTTATATTGCAGATCACATCTATGGAAATGTTCGCCAACTTGAA
GGAGCTATTAATAAGCTCACAGCATACTGTCGCTTATTTGGAAAAACACTTACAGAAAGCATTGTTCGAGATACTCTAAA
AGAGCTTTTCCGTTCTCCCTCTAAACAAAAAGTCTCTGTAGAAAGCATATTAAAAAGTGTTGCTACTGTTTTCCAAGTAA
AATTGCAGGATCTCAAAGGAAATTCACGTTCTAAAGAACTTGTTTTAGCCCGCCAAGTGGCTATGTACCTTGCCAAAACC
TTAATTACAGACTCTTTAGTTGCTATAGGTTCTGCTTTTGGAAAAACGCATTCCACGGTACTTTATGCTTGCAAAACTAT
AGAACAAAAAATAGAAAAAGATGAAACTCTAACACGTCAAATTAGTTTGTGTAAAAACCACATTGTTGGGTAA

Upstream 100 bases:

>100_bases
ATTGCTTATCAATAGCAATGGTACATTGGCACTTTCTTCCTAATCGAGCACCAGTTGCGCTCGTCTGTAAGAAAATTCTC
GACACATAAAAGAGAGCAGC

Downstream 100 bases:

>100_bases
TCTAGGAGAATTTCTAATGTTCCGAAGAACTGGAAAAAGTCCCTTTGAAGATGTTCAGACATTATATGAAGAAGAAACAC
CGGCTCATTCAAATTATTCT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLTCNNCSTWEQFVNYVKTRCSKTAFENWISPIQIIEETQEKIRLEVPNIFVQNYLLDNYKQDLCSFVPLDAQGEPALEF
VVAEIKKSPVQPVAPREQKESPAETFEGSKDFELKLNTAYRFDNFIEGPSNQFVKSAAVGIAGRPGRSYNPLFIHGGVGL
GKTHLLHAVGHYVKEHHKNLRVHCITTEAFINDLVQHLRLKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
LINLNKQIVITSDKPPGQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLHIPNEIAFYIADHIYGNVRQLE
GAINKLTAYCRLFGKTLTESIVRDTLKELFRSPSKQKVSVESILKSVATVFQVKLQDLKGNSRSKELVLARQVAMYLAKT
LITDSLVAIGSAFGKTHSTVLYACKTIEQKIEKDETLTRQISLCKNHIVG

Sequences:

>Translated_450_residues
MLTCNNCSTWEQFVNYVKTRCSKTAFENWISPIQIIEETQEKIRLEVPNIFVQNYLLDNYKQDLCSFVPLDAQGEPALEF
VVAEIKKSPVQPVAPREQKESPAETFEGSKDFELKLNTAYRFDNFIEGPSNQFVKSAAVGIAGRPGRSYNPLFIHGGVGL
GKTHLLHAVGHYVKEHHKNLRVHCITTEAFINDLVQHLRLKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
LINLNKQIVITSDKPPGQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLHIPNEIAFYIADHIYGNVRQLE
GAINKLTAYCRLFGKTLTESIVRDTLKELFRSPSKQKVSVESILKSVATVFQVKLQDLKGNSRSKELVLARQVAMYLAKT
LITDSLVAIGSAFGKTHSTVLYACKTIEQKIEKDETLTRQISLCKNHIVG
>Mature_450_residues
MLTCNNCSTWEQFVNYVKTRCSKTAFENWISPIQIIEETQEKIRLEVPNIFVQNYLLDNYKQDLCSFVPLDAQGEPALEF
VVAEIKKSPVQPVAPREQKESPAETFEGSKDFELKLNTAYRFDNFIEGPSNQFVKSAAVGIAGRPGRSYNPLFIHGGVGL
GKTHLLHAVGHYVKEHHKNLRVHCITTEAFINDLVQHLRLKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
LINLNKQIVITSDKPPGQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLHIPNEIAFYIADHIYGNVRQLE
GAINKLTAYCRLFGKTLTESIVRDTLKELFRSPSKQKVSVESILKSVATVFQVKLQDLKGNSRSKELVLARQVAMYLAKT
LITDSLVAIGSAFGKTHSTVLYACKTIEQKIEKDETLTRQISLCKNHIVG

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=464, Percent_Identity=37.2844827586207, Blast_Score=312, Evalue=3e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 51103; Mature: 51103

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTCNNCSTWEQFVNYVKTRCSKTAFENWISPIQIIEETQEKIRLEVPNIFVQNYLLDNY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KQDLCSFVPLDAQGEPALEFVVAEIKKSPVQPVAPREQKESPAETFEGSKDFELKLNTAY
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECHH
RFDNFIEGPSNQFVKSAAVGIAGRPGRSYNPLFIHGGVGLGKTHLLHAVGHYVKEHHKNL
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
RVHCITTEAFINDLVQHLRLKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LINLNKQIVITSDKPPGQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLHI
HHCCCCEEEEECCCCCCCEEHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
PNEIAFYIADHIYGNVRQLEGAINKLTAYCRLFGKTLTESIVRDTLKELFRSPSKQKVSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ESILKSVATVFQVKLQDLKGNSRSKELVLARQVAMYLAKTLITDSLVAIGSAFGKTHSTV
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LYACKTIEQKIEKDETLTRQISLCKNHIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTCNNCSTWEQFVNYVKTRCSKTAFENWISPIQIIEETQEKIRLEVPNIFVQNYLLDNY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KQDLCSFVPLDAQGEPALEFVVAEIKKSPVQPVAPREQKESPAETFEGSKDFELKLNTAY
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEECHH
RFDNFIEGPSNQFVKSAAVGIAGRPGRSYNPLFIHGGVGLGKTHLLHAVGHYVKEHHKNL
HHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCC
RVHCITTEAFINDLVQHLRLKSIDKMKNFYRSLDLLLVDDIQFLQNRQNFEEEFCNTFET
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LINLNKQIVITSDKPPGQLKLSERIIARMEWGLVAHVGIPDLETRVAILQHKAEQKGLHI
HHCCCCEEEEECCCCCCCEEHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
PNEIAFYIADHIYGNVRQLEGAINKLTAYCRLFGKTLTESIVRDTLKELFRSPSKQKVSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ESILKSVATVFQVKLQDLKGNSRSKELVLARQVAMYLAKTLITDSLVAIGSAFGKTHSTV
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LYACKTIEQKIEKDETLTRQISLCKNHIVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]