Click here to switch to the map view.

The map label for this gene is glgX [H]

Identifier: 89898407

GI number: 89898407

Start: 703404

End: 705392

Strand: Direct

Name: glgX [H]

Synonym: CF0600

Alternate gene names: 89898407

Gene position: 703404-705392 (Clockwise)

Preceding gene: 89898403

Following gene: 89898410

Centisome position: 60.31

GC content: 39.17

Gene sequence:

>1989_bases
ATGGGCAAAATCAGCTTTTATCCAGGTTCTCCGCTACCTTTGGGAGCAACTCAGCTTTCTTCTAGTCGTTATCGTTTTGC
GCTATTTTCTTCACAAGCCACTCAAGTAGTTTTGGTTCTTGCTGACAAGAATTTTCGCATCCAAGAAATTGCATTGTCCA
ACAAAGAAAACCGCACAGGAGCTATTTGGCATGTAGAAGTAGAAGGGATTTCAGATCAATGGTCTTATGCTTTTCGTGTA
GATGGCCCTATAAGCGAAACAACTAAGTTTGATTTTAACAAATACCTTTCCGATCCTTATGCAAAAAACCTTCGTTCTCC
TCAAACTTTTGGCTCTACAAAAACTTCTGGAGACTATGCCTTTAGTTATTTAAAAAATGAAGAGTTTTCTTGGGAAGGAG
ATCGCTGTCTTAATTTACCTAAAGAAGAATCAATCATTTATGAAATGCATGTGCGTTCATTTACTTGGAATAATTCTTCA
AAAGTGCGTTATCCGGGAACTTTCTTAGGAATCATTGAAAAAATAGATTATCTGAAAAAACTTGGAATTACCGCTATAGA
GCTTCTCCCTATTTTCGAATTCGATGAAACATCTCATCCTTTTCCAAGGGCCGATTACCCATATCTTTGCAATTATTGGG
GATATTCTTCTGTAAACTTTTTTTCTCCCTGTCGACGTTATGCCTACGGTTCAGATCCCTGCGCCCCAATCAGAGAATTT
AAAACTCTAGTCAAAGCTTTACATCAAGAAGGCATTGAGATAATACTTGATGTTGTTTTCAATCATACTGGAGAAAACAC
CACCTGTCCCCTACCATGGATAGATCTTCCCTCTTACTACATGATAAATTCTCAAGGGGAGCTAGCTAACTATTCAGGAT
GCGGCAATACAGTGAATACCAATCACACGCCTACAACACAATGGATTTTGGACTCTTTACGCTACTGGGTTCAGGAAATG
CACGTAGACGGTTTTCGCTTTGATCTTACTTCTATATTCTCTCGAGATCCTTTAGGGAATCCCGTTCCCTTCTCTCCTAT
TCTACACACCATTAGCTATGATCCCATACTTTCTGAAACAAAAATTATCGCTGAGCCTTGGGATGCCTCGGGTCTTTATC
AAGTAGGATATTTCCCCACCTTAAACCCACGTTGGAGTGAGTGGAACGGACCATATCGCGATACAGTAAAGTCCTTTTTA
AACGGAAATGAACATCTTATTGGATCTTTTGCCACAAGAATTTCAGGATCCCAAGATCTCTATCCCCAAGGTTCTCCGTG
TAATTCAATTAACTATATTTGTAGCCATGACGGATTTACTCTGTATGATACAGTCTCCTATAACAACAAACACAACGAGG
AAAATGGCGAGGATAATCACGACGGAAGCGATGCGAACTATAGTTATAATTTTGGAGAAGAAGGAGAAACACAGAATCCT
AAAATCATTGAATTACGCCAACGTCAGATGCGTAATTTCTTATTAACTCTATTCCTGTCTCAAGGAATTCCTATGTTGCA
ATCTGGAGATGAATATGGCCATACAGCAAAAGGAAATAACAATCGTTGGGCATTAGACACCCGTGCCAATCACTTCCTTT
GGGATGAACTATATAAAAATACTTCTCTTGTAAATTTTGTTCGTAGCGCTATTCGCTTTAGAAAACAGCATCAGGAAATC
TTCAATCAAGGATTTCTCACCCAAGAAAATATTACTTGGCTTGATGCGAATGCGACTCCCATACAGTGGAACCCCAGCAA
ATTTTTAGCCTATGAACTTAAATACAAAAATTATAGTTTATTCACAGCGTTTCATGCAGGAGAAGGGTCAGTCCAGTTCA
CCCTACCAAAACTACGAGAGAACTTTCTTCCCTATCAAAAAATTGCGGACAGCTCTTCAGGCTTCGCATCGCAAAGCCTA
TCAGAAACAACCACTTTAGACTCTTATATGATGCTAGTAGCTATTAGTTATGCAAGTCACTCTAACTAA

Upstream 100 bases:

>100_bases
TTTAAGTTAACACATTGTAGATTTGTTAGTAAACGATTTCAAAGACTTAAAATCAAAAACTTTCTTGTTTTTCTCAACCT
TTTATGCTATCGGTTTAGCG

Downstream 100 bases:

>100_bases
TTTCATGAGTTTCTTCACTCCAGGACTCTATAACAACGAAATCAGCATCTTTATAGAATTTTTCTAGGATTTGGCGCGCG
TTATACCCCTCGATAGCAAG

Product: glycogen hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MGKISFYPGSPLPLGATQLSSSRYRFALFSSQATQVVLVLADKNFRIQEIALSNKENRTGAIWHVEVEGISDQWSYAFRV
DGPISETTKFDFNKYLSDPYAKNLRSPQTFGSTKTSGDYAFSYLKNEEFSWEGDRCLNLPKEESIIYEMHVRSFTWNNSS
KVRYPGTFLGIIEKIDYLKKLGITAIELLPIFEFDETSHPFPRADYPYLCNYWGYSSVNFFSPCRRYAYGSDPCAPIREF
KTLVKALHQEGIEIILDVVFNHTGENTTCPLPWIDLPSYYMINSQGELANYSGCGNTVNTNHTPTTQWILDSLRYWVQEM
HVDGFRFDLTSIFSRDPLGNPVPFSPILHTISYDPILSETKIIAEPWDASGLYQVGYFPTLNPRWSEWNGPYRDTVKSFL
NGNEHLIGSFATRISGSQDLYPQGSPCNSINYICSHDGFTLYDTVSYNNKHNEENGEDNHDGSDANYSYNFGEEGETQNP
KIIELRQRQMRNFLLTLFLSQGIPMLQSGDEYGHTAKGNNNRWALDTRANHFLWDELYKNTSLVNFVRSAIRFRKQHQEI
FNQGFLTQENITWLDANATPIQWNPSKFLAYELKYKNYSLFTAFHAGEGSVQFTLPKLRENFLPYQKIADSSSGFASQSL
SETTTLDSYMMLVAISYASHSN

Sequences:

>Translated_662_residues
MGKISFYPGSPLPLGATQLSSSRYRFALFSSQATQVVLVLADKNFRIQEIALSNKENRTGAIWHVEVEGISDQWSYAFRV
DGPISETTKFDFNKYLSDPYAKNLRSPQTFGSTKTSGDYAFSYLKNEEFSWEGDRCLNLPKEESIIYEMHVRSFTWNNSS
KVRYPGTFLGIIEKIDYLKKLGITAIELLPIFEFDETSHPFPRADYPYLCNYWGYSSVNFFSPCRRYAYGSDPCAPIREF
KTLVKALHQEGIEIILDVVFNHTGENTTCPLPWIDLPSYYMINSQGELANYSGCGNTVNTNHTPTTQWILDSLRYWVQEM
HVDGFRFDLTSIFSRDPLGNPVPFSPILHTISYDPILSETKIIAEPWDASGLYQVGYFPTLNPRWSEWNGPYRDTVKSFL
NGNEHLIGSFATRISGSQDLYPQGSPCNSINYICSHDGFTLYDTVSYNNKHNEENGEDNHDGSDANYSYNFGEEGETQNP
KIIELRQRQMRNFLLTLFLSQGIPMLQSGDEYGHTAKGNNNRWALDTRANHFLWDELYKNTSLVNFVRSAIRFRKQHQEI
FNQGFLTQENITWLDANATPIQWNPSKFLAYELKYKNYSLFTAFHAGEGSVQFTLPKLRENFLPYQKIADSSSGFASQSL
SETTTLDSYMMLVAISYASHSN
>Mature_661_residues
GKISFYPGSPLPLGATQLSSSRYRFALFSSQATQVVLVLADKNFRIQEIALSNKENRTGAIWHVEVEGISDQWSYAFRVD
GPISETTKFDFNKYLSDPYAKNLRSPQTFGSTKTSGDYAFSYLKNEEFSWEGDRCLNLPKEESIIYEMHVRSFTWNNSSK
VRYPGTFLGIIEKIDYLKKLGITAIELLPIFEFDETSHPFPRADYPYLCNYWGYSSVNFFSPCRRYAYGSDPCAPIREFK
TLVKALHQEGIEIILDVVFNHTGENTTCPLPWIDLPSYYMINSQGELANYSGCGNTVNTNHTPTTQWILDSLRYWVQEMH
VDGFRFDLTSIFSRDPLGNPVPFSPILHTISYDPILSETKIIAEPWDASGLYQVGYFPTLNPRWSEWNGPYRDTVKSFLN
GNEHLIGSFATRISGSQDLYPQGSPCNSINYICSHDGFTLYDTVSYNNKHNEENGEDNHDGSDANYSYNFGEEGETQNPK
IIELRQRQMRNFLLTLFLSQGIPMLQSGDEYGHTAKGNNNRWALDTRANHFLWDELYKNTSLVNFVRSAIRFRKQHQEIF
NQGFLTQENITWLDANATPIQWNPSKFLAYELKYKNYSLFTAFHAGEGSVQFTLPKLRENFLPYQKIADSSSGFASQSLS
ETTTLDSYMMLVAISYASHSN

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=598, Percent_Identity=36.9565217391304, Blast_Score=349, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75814; Mature: 75683

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKISFYPGSPLPLGATQLSSSRYRFALFSSQATQVVLVLADKNFRIQEIALSNKENRTG
CCEEECCCCCCCCCCHHHHCCCCEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCCC
AIWHVEVEGISDQWSYAFRVDGPISETTKFDFNKYLSDPYAKNLRSPQTFGSTKTSGDYA
EEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCCHH
FSYLKNEEFSWEGDRCLNLPKEESIIYEMHVRSFTWNNSSKVRYPGTFLGIIEKIDYLKK
HHHHCCCCCCCCCCHHCCCCCHHCEEEEEEEEEEEECCCCCEECCHHHHHHHHHHHHHHH
LGITAIELLPIFEFDETSHPFPRADYPYLCNYWGYSSVNFFSPCRRYAYGSDPCAPIREF
CCCCEEEEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
KTLVKALHQEGIEIILDVVFNHTGENTTCPLPWIDLPSYYMINSQGELANYSGCGNTVNT
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
NHTPTTQWILDSLRYWVQEMHVDGFRFDLTSIFSRDPLGNPVPFSPILHTISYDPILSET
CCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC
KIIAEPWDASGLYQVGYFPTLNPRWSEWNGPYRDTVKSFLNGNEHLIGSFATRISGSQDL
EEEECCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHCCCCCCC
YPQGSPCNSINYICSHDGFTLYDTVSYNNKHNEENGEDNHDGSDANYSYNFGEEGETQNP
CCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
KIIELRQRQMRNFLLTLFLSQGIPMLQSGDEYGHTAKGNNNRWALDTRANHFLWDELYKN
CCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCC
TSLVNFVRSAIRFRKQHQEIFNQGFLTQENITWLDANATPIQWNPSKFLAYELKYKNYSL
CHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCEEECCCEEEEEEEEECCEEE
FTAFHAGEGSVQFTLPKLRENFLPYQKIADSSSGFASQSLSETTTLDSYMMLVAISYASH
EEEEECCCCCEEEEEHHHHHHCCCHHHHCCCCCCCHHHCCHHHHHHHHEEEHHEEEHHCC
SN
CC
>Mature Secondary Structure 
GKISFYPGSPLPLGATQLSSSRYRFALFSSQATQVVLVLADKNFRIQEIALSNKENRTG
CEEECCCCCCCCCCHHHHCCCCEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCCC
AIWHVEVEGISDQWSYAFRVDGPISETTKFDFNKYLSDPYAKNLRSPQTFGSTKTSGDYA
EEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCCCHH
FSYLKNEEFSWEGDRCLNLPKEESIIYEMHVRSFTWNNSSKVRYPGTFLGIIEKIDYLKK
HHHHCCCCCCCCCCHHCCCCCHHCEEEEEEEEEEEECCCCCEECCHHHHHHHHHHHHHHH
LGITAIELLPIFEFDETSHPFPRADYPYLCNYWGYSSVNFFSPCRRYAYGSDPCAPIREF
CCCCEEEEEEEEECCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
KTLVKALHQEGIEIILDVVFNHTGENTTCPLPWIDLPSYYMINSQGELANYSGCGNTVNT
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
NHTPTTQWILDSLRYWVQEMHVDGFRFDLTSIFSRDPLGNPVPFSPILHTISYDPILSET
CCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC
KIIAEPWDASGLYQVGYFPTLNPRWSEWNGPYRDTVKSFLNGNEHLIGSFATRISGSQDL
EEEECCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHCCCCCCC
YPQGSPCNSINYICSHDGFTLYDTVSYNNKHNEENGEDNHDGSDANYSYNFGEEGETQNP
CCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
KIIELRQRQMRNFLLTLFLSQGIPMLQSGDEYGHTAKGNNNRWALDTRANHFLWDELYKN
CCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCC
TSLVNFVRSAIRFRKQHQEIFNQGFLTQENITWLDANATPIQWNPSKFLAYELKYKNYSL
CHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCEEECCCEEEEEEEEECCEEE
FTAFHAGEGSVQFTLPKLRENFLPYQKIADSSSGFASQSLSETTTLDSYMMLVAISYASH
EEEEECCCCCEEEEEHHHHHHCCCHHHHCCCCCCCHHHCCHHHHHHHHEEEHHEEEHHCC
SN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]