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The map label for this gene is lepA [H]

Identifier: 89898382

GI number: 89898382

Start: 674222

End: 676030

Strand: Direct

Name: lepA [H]

Synonym: CF0575

Alternate gene names: 89898382

Gene position: 674222-676030 (Clockwise)

Preceding gene: 89898380

Following gene: 89898388

Centisome position: 57.81

GC content: 38.58

Gene sequence:

>1809_bases
TTGAAAGAGTATAAATTAGAAAATATTCGAAACTTTTCTATAATTGCGCATATTGATCACGGAAAATCCACAATTGCTGA
TCGCCTACTTGAAAGTACGAGTACCGTTGAGCAAAGAGAGATGCGTGAGCAGCTTCTTGATTCTATGGACCTTGAAAGGG
AGCGTGGTATCACCATCAAAGCACATCCCGTGACTATGTATTATAACTACAACGGTGAAGTCTATCAGCTTAACCTCATA
GATACGCCGGGCCACGTGGATTTTTCTTATGAAGTATCGCGATCTTTAGCAGCTTGTGAGGGAGCTCTTCTTATTGTTGA
CGCAGCTCAAGGTGTGCAAGCTCAGAGTCTAGCTAATGTGTATCTAGCCTTAGAACGAGATCTAGAAATCATCCCCATTT
TAAATAAGATTGATCTACCTGCTGCCAACCCTGAAAAAATCCGAAAACAAATAGAAGATTACATAGGTCTGGATACAACT
CATGCAATTGCTTGCTCAGCAAAAACTGGTGAGGGAATTTCAGAAATTCTTGAGGCAATCATTAACCTGATTCCTCCGCC
AACTCCCCCTCAAGAAACTGAATTAAAAGCTTTAATTTTTGATTCCCATTATGATCCTTATGTAGGAATTATGGTGTATG
TTCGTGTGATCAGCGGAGAAATTAAAAAGGGTGATCGCATTACTTTTATGGCAACAAAAGGATCCGCTTTTGAAGTATTG
GGTGTTGGAGCTTTTCTTCCTGAAGCCACTTTAATTGAAGGGTCTTTACGAGCCGGTCAGGTGGGTTACTTCATAGCCAA
TCTTAAAAAAGTTAAAGATGTAAAAATTGGTGATACTGTAACTACTGTAAAACATCCGGCTAAAGTACCTTTAGATGGGT
TTAAGGAAATTAATCCTGTAGTTTTCGCTGGTATCTACCCTATCGACTCCTCAGATTTTGATACTTTAAAAGATGCTTTA
AGTCGTTTGCAACTAAACGACTCAGCTCTAACAATCGAGCAAGAAAGCAGCCATTCTTTAGGCTTTGGCTTCCGTTGTGG
CTTTTTAGGATTGCTACATTTAGAGATTGTCTTTGAAAGAATCATCAGAGAATTTGATCTTGATATTATTGCCACAGCGC
CCAGTGTGATTTATAAAGTCGTCTTAAAAAATGGAAAAACACTGCTCATAGACAATCCAACGGCCTATCCTGATCCTTCG
ATTATCGAGCATATGGAAGAACCTTGGGTACATGTAAATATCATCACTCCTCAGGAGTACTTAAGCAGTATTATGAACCT
ATGCTTGGATAAGCGTGGTGTATGCTTAAAAACCGAGATGCTCGATCAACATAGGCTAGTTCTTTCTTATGATCTTCCTT
TAAACGAAATTGTTTCTGATTTTAATGACAAGTTAAAATCAGTAACCAAGGGTTACGGATCTTTTGATTATCGTTTAGGA
GATTATCGCAAGGGAGCTATCATAAAGCTTGAAATTCTAATCAATGACGAGCCAGTCGATGCTTTTTCTTGTCTCGTACA
TAGGGATAAGGCAGAGGCTCGCGGAAGAAGTATCTGCGAAAAGTTAGTTGATGTCATTCCTCAACAACTATTTAAAATTC
CCATCCAAGCTGCTATTAATAAAAAAGTGATTGCTCGGGAAACAATTCGCGCTCTTTCAAAGAATGTCACAGCAAAGTGT
TATGGTGGGGACATCACTAGAAAACGCAAGCTTTGGGAAAAACAAAAAAAGGGTAAAAAGCGTATGAAAGAATTTGGGAA
AGTCTCTATCCCAAATACAGCTTTTATTGAAGTTCTAAAAATAGATTAA

Upstream 100 bases:

>100_bases
ATAAATTAAACTCATCTTCTTATATTCTAGTAGTATTGACAACTAACTATTCTCAGTCTAACATGGCTCTCTGTGAAATT
GACAAAACATCTGCCGTATT

Downstream 100 bases:

>100_bases
CCCTACTCCAATAAATTAGAGCGCTTTTCTATAAAGGGAGCCCGGTTCCCTAGCAAATCTCTACTTCTTTTTATTACTCC
AACAATCAAGACCCTTGAGG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MKEYKLENIRNFSIIAHIDHGKSTIADRLLESTSTVEQREMREQLLDSMDLERERGITIKAHPVTMYYNYNGEVYQLNLI
DTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPILNKIDLPAANPEKIRKQIEDYIGLDTT
HAIACSAKTGEGISEILEAIINLIPPPTPPQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSAFEVL
GVGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKVPLDGFKEINPVVFAGIYPIDSSDFDTLKDAL
SRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIVFERIIREFDLDIIATAPSVIYKVVLKNGKTLLIDNPTAYPDPS
IIEHMEEPWVHVNIITPQEYLSSIMNLCLDKRGVCLKTEMLDQHRLVLSYDLPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYRKGAIIKLEILINDEPVDAFSCLVHRDKAEARGRSICEKLVDVIPQQLFKIPIQAAINKKVIARETIRALSKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLKID

Sequences:

>Translated_602_residues
MKEYKLENIRNFSIIAHIDHGKSTIADRLLESTSTVEQREMREQLLDSMDLERERGITIKAHPVTMYYNYNGEVYQLNLI
DTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPILNKIDLPAANPEKIRKQIEDYIGLDTT
HAIACSAKTGEGISEILEAIINLIPPPTPPQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSAFEVL
GVGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKVPLDGFKEINPVVFAGIYPIDSSDFDTLKDAL
SRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIVFERIIREFDLDIIATAPSVIYKVVLKNGKTLLIDNPTAYPDPS
IIEHMEEPWVHVNIITPQEYLSSIMNLCLDKRGVCLKTEMLDQHRLVLSYDLPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYRKGAIIKLEILINDEPVDAFSCLVHRDKAEARGRSICEKLVDVIPQQLFKIPIQAAINKKVIARETIRALSKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLKID
>Mature_602_residues
MKEYKLENIRNFSIIAHIDHGKSTIADRLLESTSTVEQREMREQLLDSMDLERERGITIKAHPVTMYYNYNGEVYQLNLI
DTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPILNKIDLPAANPEKIRKQIEDYIGLDTT
HAIACSAKTGEGISEILEAIINLIPPPTPPQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSAFEVL
GVGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKVPLDGFKEINPVVFAGIYPIDSSDFDTLKDAL
SRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIVFERIIREFDLDIIATAPSVIYKVVLKNGKTLLIDNPTAYPDPS
IIEHMEEPWVHVNIITPQEYLSSIMNLCLDKRGVCLKTEMLDQHRLVLSYDLPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYRKGAIIKLEILINDEPVDAFSCLVHRDKAEARGRSICEKLVDVIPQQLFKIPIQAAINKKVIARETIRALSKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLKID

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=49.0131578947368, Blast_Score=605, Evalue=1e-173,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=41.6666666666667, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI18390331, Length=205, Percent_Identity=33.1707317073171, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=36.734693877551, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI25306283, Length=151, Percent_Identity=41.0596026490066, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923640, Length=151, Percent_Identity=41.0596026490066, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI25306287, Length=151, Percent_Identity=41.0596026490066, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=41.2844036697248, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=41.2844036697248, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=41.2844036697248, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=38.9705882352941, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=38.9705882352941, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI53729339, Length=225, Percent_Identity=26.2222222222222, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI53729337, Length=225, Percent_Identity=26.2222222222222, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI94966752, Length=93, Percent_Identity=37.6344086021505, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI4503471, Length=344, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=50.5016722408027, Blast_Score=623, Evalue=1e-179,
Organism=Escherichia coli, GI48994988, Length=497, Percent_Identity=26.7605633802817, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=36.7741935483871, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=167, Percent_Identity=31.7365269461078, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789559, Length=290, Percent_Identity=25.5172413793103, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=620, Percent_Identity=40, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17556745, Length=459, Percent_Identity=25.4901960784314, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=38.7755102040816, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=212, Percent_Identity=30.6603773584906, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=212, Percent_Identity=30.6603773584906, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=34.1772151898734, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=607, Percent_Identity=43.163097199341, Blast_Score=498, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6323098, Length=153, Percent_Identity=37.2549019607843, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=37.4149659863946, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=37.4149659863946, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=37.719298245614, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=36.8794326241135, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=43.4494195688226, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24582462, Length=153, Percent_Identity=39.8692810457516, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=40.8450704225352, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=36.4238410596026, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=36.4238410596026, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=36.4238410596026, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357743, Length=167, Percent_Identity=34.7305389221557, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=37.3333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=227, Percent_Identity=29.9559471365639, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363316, Length=290, Percent_Identity=25.5172413793103, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864358, Length=290, Percent_Identity=25.5172413793103, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67451; Mature: 67451

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYKLENIRNFSIIAHIDHGKSTIADRLLESTSTVEQREMREQLLDSMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEE
AHPVTMYYNYNGEVYQLNLIDTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANV
EEEEEEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YLALERDLEIIPILNKIDLPAANPEKIRKQIEDYIGLDTTHAIACSAKTGEGISEILEAI
HEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
INLIPPPTPPQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSAFEVL
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEE
GVGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKVPLDGFKEINPV
ECHHHCCCCCEEECCCCCCCHHHHHHHHHHHCEEECCCCEEECCCCCCCCCCCHHCCCCE
VFAGIYPIDSSDFDTLKDALSRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIVFER
EEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
IIREFDLDIIATAPSVIYKVVLKNGKTLLIDNPTAYPDPSIIEHMEEPWVHVNIITPQEY
HHHHHCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCCEEEEEECHHHH
LSSIMNLCLDKRGVCLKTEMLDQHRLVLSYDLPLNEIVSDFNDKLKSVTKGYGSFDYRLG
HHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEECCCC
DYRKGAIIKLEILINDEPVDAFSCLVHRDKAEARGRSICEKLVDVIPQQLFKIPIQAAIN
CCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
KKVIARETIRALSKNVTAKCYGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLK
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
ID
EC
>Mature Secondary Structure
MKEYKLENIRNFSIIAHIDHGKSTIADRLLESTSTVEQREMREQLLDSMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEE
AHPVTMYYNYNGEVYQLNLIDTPGHVDFSYEVSRSLAACEGALLIVDAAQGVQAQSLANV
EEEEEEEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
YLALERDLEIIPILNKIDLPAANPEKIRKQIEDYIGLDTTHAIACSAKTGEGISEILEAI
HEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
INLIPPPTPPQETELKALIFDSHYDPYVGIMVYVRVISGEIKKGDRITFMATKGSAFEVL
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEE
GVGAFLPEATLIEGSLRAGQVGYFIANLKKVKDVKIGDTVTTVKHPAKVPLDGFKEINPV
ECHHHCCCCCEEECCCCCCCHHHHHHHHHHHCEEECCCCEEECCCCCCCCCCCHHCCCCE
VFAGIYPIDSSDFDTLKDALSRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIVFER
EEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
IIREFDLDIIATAPSVIYKVVLKNGKTLLIDNPTAYPDPSIIEHMEEPWVHVNIITPQEY
HHHHHCCEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCCCEEEEEECHHHH
LSSIMNLCLDKRGVCLKTEMLDQHRLVLSYDLPLNEIVSDFNDKLKSVTKGYGSFDYRLG
HHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEECCCC
DYRKGAIIKLEILINDEPVDAFSCLVHRDKAEARGRSICEKLVDVIPQQLFKIPIQAAIN
CCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
KKVIARETIRALSKNVTAKCYGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLK
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
ID
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA