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The map label for this gene is truB [H]

Identifier: 89898351

GI number: 89898351

Start: 640400

End: 641107

Strand: Direct

Name: truB [H]

Synonym: CF0544

Alternate gene names: 89898351

Gene position: 640400-641107 (Clockwise)

Preceding gene: 89898350

Following gene: 89898352

Centisome position: 54.91

GC content: 38.98

Gene sequence:

>708_bases
ATGGAACTTGCTACAGAACTTAAAGAAGGAATTCTTCTAGTAGATAAGCCTCAGGGTAGAACCTCGTTTAGTCTCATTCG
CACTTTAACAAAGTTAATCGGGGTCAAAAAAATCGGTCACGCAGGGACCCTGGATCCTTTTGCTACCGGCGTGATGGTTA
TGTTAATCGGTCGTAAATTTACACGGCTTTCTGATGTTCTATTATTTGAGGATAAGGAATACGCTGCAATTGCTCATCTA
GGGACAACCACAGATTCCTACGATTGTGATGGTAAAATTGTGGGGAGATCCAAAAAAGTTCCTACTTATGAGGAAATATT
AGAAGCAGCTTTGTATTTTCAGGGAGAAATCCAGCAAATTCCCCCAATGTTTTCTGCGAAAAAAATTAACGGAAAAAAGC
TCTATGAGTATGCCCGACAAGGATTATCCATAGAACGTCGCCAATCTACGGTTCAGGTAAGCTTACAAATTACAAAATAC
GAGTATCCCCTGCTATACTTTTCTGTACAATGTAGTAAAGGAACATATATTCGTAGTATTGCTCATGAACTAGGAAACAT
GTTAGGGTGTGGAGCTTATTTAGAAGAACTTCGACGTTTACGTAGTGGGAGTTTTTCTATAGATCAATGTATTGACGGGT
GTCTTTTAGATCGGCCAGACTTCGATGTATCTCCTTATTTAAGAGATTTCAATGGAAACATTCTATAG

Upstream 100 bases:

>100_bases
TTCTCCACAGGATCATATAGAAAACTTGCTTTGGAAAATACGCGAGCAAGATAAAAATTAATTCATCTGCCTAGAAAGAT
CTAACATTAACGACATCATT

Downstream 100 bases:

>100_bases
TTTAACATCGATCCATTCTTCCGTGGATTCTATAACTATAGGTTTTTTTGATGGATGCCACTTAGGCCATAAGAAATTAC
TCTCTGTATTGTCATCCTAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MELATELKEGILLVDKPQGRTSFSLIRTLTKLIGVKKIGHAGTLDPFATGVMVMLIGRKFTRLSDVLLFEDKEYAAIAHL
GTTTDSYDCDGKIVGRSKKVPTYEEILEAALYFQGEIQQIPPMFSAKKINGKKLYEYARQGLSIERRQSTVQVSLQITKY
EYPLLYFSVQCSKGTYIRSIAHELGNMLGCGAYLEELRRLRSGSFSIDQCIDGCLLDRPDFDVSPYLRDFNGNIL

Sequences:

>Translated_235_residues
MELATELKEGILLVDKPQGRTSFSLIRTLTKLIGVKKIGHAGTLDPFATGVMVMLIGRKFTRLSDVLLFEDKEYAAIAHL
GTTTDSYDCDGKIVGRSKKVPTYEEILEAALYFQGEIQQIPPMFSAKKINGKKLYEYARQGLSIERRQSTVQVSLQITKY
EYPLLYFSVQCSKGTYIRSIAHELGNMLGCGAYLEELRRLRSGSFSIDQCIDGCLLDRPDFDVSPYLRDFNGNIL
>Mature_235_residues
MELATELKEGILLVDKPQGRTSFSLIRTLTKLIGVKKIGHAGTLDPFATGVMVMLIGRKFTRLSDVLLFEDKEYAAIAHL
GTTTDSYDCDGKIVGRSKKVPTYEEILEAALYFQGEIQQIPPMFSAKKINGKKLYEYARQGLSIERRQSTVQVSLQITKY
EYPLLYFSVQCSKGTYIRSIAHELGNMLGCGAYLEELRRLRSGSFSIDQCIDGCLLDRPDFDVSPYLRDFNGNIL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=228, Percent_Identity=33.7719298245614, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI4503337, Length=207, Percent_Identity=30.9178743961353, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI215599015, Length=207, Percent_Identity=30.9178743961353, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI2367200, Length=203, Percent_Identity=41.871921182266, Blast_Score=162, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17553978, Length=206, Percent_Identity=29.126213592233, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=30.2439024390244, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=172, Percent_Identity=33.7209302325581, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364189, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364187, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364185, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281364183, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI62471759, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17975520, Length=197, Percent_Identity=31.9796954314721, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26428; Mature: 26428

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELATELKEGILLVDKPQGRTSFSLIRTLTKLIGVKKIGHAGTLDPFATGVMVMLIGRKF
CCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TRLSDVLLFEDKEYAAIAHLGTTTDSYDCDGKIVGRSKKVPTYEEILEAALYFQGEIQQI
HHHHHHHEECCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCHHHC
PPMFSAKKINGKKLYEYARQGLSIERRQSTVQVSLQITKYEYPLLYFSVQCSKGTYIRSI
CCCCCCCCCCHHHHHHHHHCCCCCHHCCCEEEEEEEEEEEECCEEEEEEECCCCHHHHHH
AHELGNMLGCGAYLEELRRLRSGSFSIDQCIDGCLLDRPDFDVSPYLRDFNGNIL
HHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MELATELKEGILLVDKPQGRTSFSLIRTLTKLIGVKKIGHAGTLDPFATGVMVMLIGRKF
CCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
TRLSDVLLFEDKEYAAIAHLGTTTDSYDCDGKIVGRSKKVPTYEEILEAALYFQGEIQQI
HHHHHHHEECCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCHHHC
PPMFSAKKINGKKLYEYARQGLSIERRQSTVQVSLQITKYEYPLLYFSVQCSKGTYIRSI
CCCCCCCCCCHHHHHHHHHCCCCCHHCCCEEEEEEEEEEEECCEEEEEEECCCCHHHHHH
AHELGNMLGCGAYLEELRRLRSGSFSIDQCIDGCLLDRPDFDVSPYLRDFNGNIL
HHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA