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The map label for this gene is leuS [H]

Identifier: 89898198

GI number: 89898198

Start: 462567

End: 465029

Strand: Direct

Name: leuS [H]

Synonym: CF0391

Alternate gene names: 89898198

Gene position: 462567-465029 (Clockwise)

Preceding gene: 89898195

Following gene: 89898199

Centisome position: 39.66

GC content: 42.02

Gene sequence:

>2463_bases
ATGCGGTACGATCCTAGTTTGATAGAAAAGAAGTGGCAAGAATTTTGGAAGGAACACAAGAGCTTCAAGGCGAACGAAGC
TGATGATAAACCTAAATATTATGTATTAGACATGTTCCCTTATCCATCGGGAGCAGGACTGCACGTCGGACATTTAATCG
GTTATACCGCCACGGATATTGTCGCTCGCTATAAGCGAGCTAAAGGATTTTCTGTTTTACATCCTATGGGGTGGGACAGT
TTCGGATTGCCTGCGGAACAATATGCTGTCAGAACGGGAACACATCCTAGAGAAACAACTCAAAAGAATATAGAAAATTT
TAGAAAGCAACTTTCTGCTATGGGATTTTCTTATGATGAGGGTAGAGAGTTCGCTACGAGTGATCCCGAATATTATCGTT
GGACGCAGAAACTTTTTCTTTTACTTTACGAAAAAGGCCTAGCTTATATGGCAGATATGGCGGTCAACTATTGCCCAGAG
TTGGGGACTGTCTTATCCAACGAAGAGGTGGAAAATGGCTTATCTATAGAGGGAGGGTATCCTGTAGAGCGCCGAATGTT
GCGTCAATGGATTTTACGGATCACAGCTTATTCTGATCAGCTTTTAGAAGGTCTAGAGGGCTTAGACTGGCCAGAAAATG
TTAAGCAGCTTCAAAGAAATTGGATAGGGAAGTCTGAAGGGGCTCTCGTACGCTTTAAAGTGAATAATCAAAACTTTTTG
GAGGTATTCACAACACGTCCTGATACTTTATGTGGTGTATCATTTTTGGTCATTGCTCCAGAGCACCCTGAGGTTGGTCA
ATTAATTGCTGAGGATCGGAGAGAAGAAGTAGAGGCTTATATTTGCCGCGCTCAAAGTAAAAGTGAAAGAGATCGTATAA
GCGAGAGCAAAATAAAAAGCGGAGTATTTACAGGAACCTATGCTAAGCATCCTATCACGGGAGCAGATATTCCTATTTGG
GTTTCCGACTACGTTATCCTTGGTTATGGCTCTGGAGTGGTTATGGGCGTTCCTGCTCACGATGAAAGAGACAGGGAGTT
TGCCGAAACTTTTTCTCTGCCTATTTATGAAGTTCTTGATACAGATGGATATTGCATTCATAGTAATCACGGAGACTTTC
TTTTAGACGGTCTTTGGGGGCGGGAAGCTCAGGATTATGTCATTGCTTATCTGCAGAAAAAGAATCTAGGGGAGTCTAAG
GTTGCGTATAAACTGCGCGATTGGCTGTTTTCTCGTCAAAGATATTGGGGAGAGCCGATTCCTATTATTCATTTTGAAGA
CGGTACGTGTCGTCCTTTGGAAGACGATGAGCTTCCTTTACTTCCTCCAGAAATTCAAGACTACCGTCCCGAAGGTTTTG
GCCAGGGGCCATTAGCAAAAGTAAAAGAGTGGGTAGATATTCACGATAAGAAAACCAATCGTCAGGGGAGACGCGAGACG
CATACCATGCCTCAATGGGCTGGGTCTTGTTGGTATTATTTGCGTTTTTGCGACGCGCTGAATTCGCAAGCACCCTGGTC
TAATGAAAAAGAAAAATATTGGATGCCTGTAGACTTGTATATTGGGGGGGCTGAACATGCTGTTTTGCACTTATTGTATT
CCCGTTTTTGGCACCGAGTGTTTTATGATGCAGGCATGGTTTCCACTCCAGAACCATTTAAAAAGTTGATAAATCAAGGC
TTAGTGCTTGCAACTTCTTATCGGATTCCTGGTAAGGGATATGTCTATCCTGAAGATGCTCATGAGGATAATGGGGTATG
GATGAGTACTTCCGGAGAGGAACTAGAAGTTCGTCAGGAAAAGATGTCTAAATCAAAGTTAAATGGAGTTGATCCTCAAA
TTCTTATCGAAGAATTCGGAGCAGATGCTTTGCGTATGTACGCTATGTTTTCTGGTCCGCTAGATAAAAATAAGCTTTGG
TGTAATCAAGGCGTTTCTGGTTGCCGACGCTTCCTCAATCGTTTTTATGAATTGGTCACGTCGTCTTTAGTTCAAGATAT
TGATGATCCTAAGGGAATGTATTTAGCCCATAGATTAGTACATCGCGTAGGGGAAGATATTGAAAAAATGTCTTTAAACA
CCATCCCATCTTCTTTTATGGAATTCATAAATGAGTTTGTGAAGCTTGATATTTATCCTAAATCTGCTTTAGCTATGGTT
GTGCGGGCTTTAGCACCTATAGCACCTCATATCAGTGAGGAATTATGGACTATTTTAGGCAATGCTCCTGGTATAGATCA
GGCTGGATGGCCAGAGGTGGATCCTAAATATTTGGAAGATACTTCGGTAACATTTGTCATTCAGGTAAATGGGAAGTTAC
GAGCGCGTCTGGATATTAGTAAGAACGCGGCGCGGGAAGAAGTTTTATCTTTAGCACGAGAGGCTGTCTCTAGGTATCTA
GAAGATAAAGAAGTGAAGAAGGAAATTTTCGTTCCAAATAGATTAGTGAATTTTGTGCTATGA

Upstream 100 bases:

>100_bases
AATCTTTTAAGAACTCTGATTAGAAGATTCTTGTGTTTTCATTGAGTGAGAGATACACTAGCCTCTATTTTATTTTAACA
GATCTCTTTGTCAGGATATT

Downstream 100 bases:

>100_bases
TCAAACGACGACTAACTAAGTTACATACCTTCCTATACGATTGCTTTTTGATCTTGGCGTTTATAGTTGCGCTTCCTAGG
ATTCTCTATAAAAGATTGGT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MRYDPSLIEKKWQEFWKEHKSFKANEADDKPKYYVLDMFPYPSGAGLHVGHLIGYTATDIVARYKRAKGFSVLHPMGWDS
FGLPAEQYAVRTGTHPRETTQKNIENFRKQLSAMGFSYDEGREFATSDPEYYRWTQKLFLLLYEKGLAYMADMAVNYCPE
LGTVLSNEEVENGLSIEGGYPVERRMLRQWILRITAYSDQLLEGLEGLDWPENVKQLQRNWIGKSEGALVRFKVNNQNFL
EVFTTRPDTLCGVSFLVIAPEHPEVGQLIAEDRREEVEAYICRAQSKSERDRISESKIKSGVFTGTYAKHPITGADIPIW
VSDYVILGYGSGVVMGVPAHDERDREFAETFSLPIYEVLDTDGYCIHSNHGDFLLDGLWGREAQDYVIAYLQKKNLGESK
VAYKLRDWLFSRQRYWGEPIPIIHFEDGTCRPLEDDELPLLPPEIQDYRPEGFGQGPLAKVKEWVDIHDKKTNRQGRRET
HTMPQWAGSCWYYLRFCDALNSQAPWSNEKEKYWMPVDLYIGGAEHAVLHLLYSRFWHRVFYDAGMVSTPEPFKKLINQG
LVLATSYRIPGKGYVYPEDAHEDNGVWMSTSGEELEVRQEKMSKSKLNGVDPQILIEEFGADALRMYAMFSGPLDKNKLW
CNQGVSGCRRFLNRFYELVTSSLVQDIDDPKGMYLAHRLVHRVGEDIEKMSLNTIPSSFMEFINEFVKLDIYPKSALAMV
VRALAPIAPHISEELWTILGNAPGIDQAGWPEVDPKYLEDTSVTFVIQVNGKLRARLDISKNAAREEVLSLAREAVSRYL
EDKEVKKEIFVPNRLVNFVL

Sequences:

>Translated_820_residues
MRYDPSLIEKKWQEFWKEHKSFKANEADDKPKYYVLDMFPYPSGAGLHVGHLIGYTATDIVARYKRAKGFSVLHPMGWDS
FGLPAEQYAVRTGTHPRETTQKNIENFRKQLSAMGFSYDEGREFATSDPEYYRWTQKLFLLLYEKGLAYMADMAVNYCPE
LGTVLSNEEVENGLSIEGGYPVERRMLRQWILRITAYSDQLLEGLEGLDWPENVKQLQRNWIGKSEGALVRFKVNNQNFL
EVFTTRPDTLCGVSFLVIAPEHPEVGQLIAEDRREEVEAYICRAQSKSERDRISESKIKSGVFTGTYAKHPITGADIPIW
VSDYVILGYGSGVVMGVPAHDERDREFAETFSLPIYEVLDTDGYCIHSNHGDFLLDGLWGREAQDYVIAYLQKKNLGESK
VAYKLRDWLFSRQRYWGEPIPIIHFEDGTCRPLEDDELPLLPPEIQDYRPEGFGQGPLAKVKEWVDIHDKKTNRQGRRET
HTMPQWAGSCWYYLRFCDALNSQAPWSNEKEKYWMPVDLYIGGAEHAVLHLLYSRFWHRVFYDAGMVSTPEPFKKLINQG
LVLATSYRIPGKGYVYPEDAHEDNGVWMSTSGEELEVRQEKMSKSKLNGVDPQILIEEFGADALRMYAMFSGPLDKNKLW
CNQGVSGCRRFLNRFYELVTSSLVQDIDDPKGMYLAHRLVHRVGEDIEKMSLNTIPSSFMEFINEFVKLDIYPKSALAMV
VRALAPIAPHISEELWTILGNAPGIDQAGWPEVDPKYLEDTSVTFVIQVNGKLRARLDISKNAAREEVLSLAREAVSRYL
EDKEVKKEIFVPNRLVNFVL
>Mature_820_residues
MRYDPSLIEKKWQEFWKEHKSFKANEADDKPKYYVLDMFPYPSGAGLHVGHLIGYTATDIVARYKRAKGFSVLHPMGWDS
FGLPAEQYAVRTGTHPRETTQKNIENFRKQLSAMGFSYDEGREFATSDPEYYRWTQKLFLLLYEKGLAYMADMAVNYCPE
LGTVLSNEEVENGLSIEGGYPVERRMLRQWILRITAYSDQLLEGLEGLDWPENVKQLQRNWIGKSEGALVRFKVNNQNFL
EVFTTRPDTLCGVSFLVIAPEHPEVGQLIAEDRREEVEAYICRAQSKSERDRISESKIKSGVFTGTYAKHPITGADIPIW
VSDYVILGYGSGVVMGVPAHDERDREFAETFSLPIYEVLDTDGYCIHSNHGDFLLDGLWGREAQDYVIAYLQKKNLGESK
VAYKLRDWLFSRQRYWGEPIPIIHFEDGTCRPLEDDELPLLPPEIQDYRPEGFGQGPLAKVKEWVDIHDKKTNRQGRRET
HTMPQWAGSCWYYLRFCDALNSQAPWSNEKEKYWMPVDLYIGGAEHAVLHLLYSRFWHRVFYDAGMVSTPEPFKKLINQG
LVLATSYRIPGKGYVYPEDAHEDNGVWMSTSGEELEVRQEKMSKSKLNGVDPQILIEEFGADALRMYAMFSGPLDKNKLW
CNQGVSGCRRFLNRFYELVTSSLVQDIDDPKGMYLAHRLVHRVGEDIEKMSLNTIPSSFMEFINEFVKLDIYPKSALAMV
VRALAPIAPHISEELWTILGNAPGIDQAGWPEVDPKYLEDTSVTFVIQVNGKLRARLDISKNAAREEVLSLAREAVSRYL
EDKEVKKEIFVPNRLVNFVL

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=870, Percent_Identity=34.5977011494253, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI94721239, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI94721241, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786861, Length=871, Percent_Identity=41.4466130884041, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1790708, Length=379, Percent_Identity=25.065963060686, Blast_Score=93, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71997510, Length=815, Percent_Identity=28.4662576687117, Blast_Score=285, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71997517, Length=812, Percent_Identity=28.5714285714286, Blast_Score=284, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI212645227, Length=375, Percent_Identity=28.2666666666667, Blast_Score=131, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323414, Length=876, Percent_Identity=34.4748858447489, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321531, Length=418, Percent_Identity=25.3588516746411, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21355409, Length=869, Percent_Identity=33.0264672036824, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17864482, Length=445, Percent_Identity=23.8202247191011, Blast_Score=97, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24653289, Length=445, Percent_Identity=23.8202247191011, Blast_Score=97, Evalue=6e-20,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 94248; Mature: 94248

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYDPSLIEKKWQEFWKEHKSFKANEADDKPKYYVLDMFPYPSGAGLHVGHLIGYTATDI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
VARYKRAKGFSVLHPMGWDSFGLPAEQYAVRTGTHPRETTQKNIENFRKQLSAMGFSYDE
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GREFATSDPEYYRWTQKLFLLLYEKGLAYMADMAVNYCPELGTVLSNEEVENGLSIEGGY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHCCCCCCCCC
PVERRMLRQWILRITAYSDQLLEGLEGLDWPENVKQLQRNWIGKSEGALVRFKVNNQNFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEE
EVFTTRPDTLCGVSFLVIAPEHPEVGQLIAEDRREEVEAYICRAQSKSERDRISESKIKS
EEEECCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GVFTGTYAKHPITGADIPIWVSDYVILGYGSGVVMGVPAHDERDREFAETFSLPIYEVLD
CCEECCCCCCCCCCCCCCEEEECEEEEEECCCEEEECCCCCCHHHHHHHHHCCCHHHHHC
TDGYCIHSNHGDFLLDGLWGREAQDYVIAYLQKKNLGESKVAYKLRDWLFSRQRYWGEPI
CCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
PIIHFEDGTCRPLEDDELPLLPPEIQDYRPEGFGQGPLAKVKEWVDIHDKKTNRQGRRET
CEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
HTMPQWAGSCWYYLRFCDALNSQAPWSNEKEKYWMPVDLYIGGAEHAVLHLLYSRFWHRV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHH
FYDAGMVSTPEPFKKLINQGLVLATSYRIPGKGYVYPEDAHEDNGVWMSTSGEELEVRQE
HHCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHH
KMSKSKLNGVDPQILIEEFGADALRMYAMFSGPLDKNKLWCNQGVSGCRRFLNRFYELVT
HHHHHHCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
SSLVQDIDDPKGMYLAHRLVHRVGEDIEKMSLNTIPSSFMEFINEFVKLDIYPKSALAMV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHH
VRALAPIAPHISEELWTILGNAPGIDQAGWPEVDPKYLEDTSVTFVIQVNGKLRARLDIS
HHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEECCCEEEEEECC
KNAAREEVLSLAREAVSRYLEDKEVKKEIFVPNRLVNFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure
MRYDPSLIEKKWQEFWKEHKSFKANEADDKPKYYVLDMFPYPSGAGLHVGHLIGYTATDI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
VARYKRAKGFSVLHPMGWDSFGLPAEQYAVRTGTHPRETTQKNIENFRKQLSAMGFSYDE
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GREFATSDPEYYRWTQKLFLLLYEKGLAYMADMAVNYCPELGTVLSNEEVENGLSIEGGY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCHHHHCCCCCCCCC
PVERRMLRQWILRITAYSDQLLEGLEGLDWPENVKQLQRNWIGKSEGALVRFKVNNQNFL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEE
EVFTTRPDTLCGVSFLVIAPEHPEVGQLIAEDRREEVEAYICRAQSKSERDRISESKIKS
EEEECCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GVFTGTYAKHPITGADIPIWVSDYVILGYGSGVVMGVPAHDERDREFAETFSLPIYEVLD
CCEECCCCCCCCCCCCCCEEEECEEEEEECCCEEEECCCCCCHHHHHHHHHCCCHHHHHC
TDGYCIHSNHGDFLLDGLWGREAQDYVIAYLQKKNLGESKVAYKLRDWLFSRQRYWGEPI
CCCEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
PIIHFEDGTCRPLEDDELPLLPPEIQDYRPEGFGQGPLAKVKEWVDIHDKKTNRQGRRET
CEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHH
HTMPQWAGSCWYYLRFCDALNSQAPWSNEKEKYWMPVDLYIGGAEHAVLHLLYSRFWHRV
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHH
FYDAGMVSTPEPFKKLINQGLVLATSYRIPGKGYVYPEDAHEDNGVWMSTSGEELEVRQE
HHCCCCCCCCHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHHHH
KMSKSKLNGVDPQILIEEFGADALRMYAMFSGPLDKNKLWCNQGVSGCRRFLNRFYELVT
HHHHHHCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
SSLVQDIDDPKGMYLAHRLVHRVGEDIEKMSLNTIPSSFMEFINEFVKLDIYPKSALAMV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHH
VRALAPIAPHISEELWTILGNAPGIDQAGWPEVDPKYLEDTSVTFVIQVNGKLRARLDIS
HHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEECCCEEEEEECC
KNAAREEVLSLAREAVSRYLEDKEVKKEIFVPNRLVNFVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]