Click here to switch to the map view.

The map label for this gene is metG

Identifier: 89898167

GI number: 89898167

Start: 421169

End: 422824

Strand: Direct

Name: metG

Synonym: CF0360

Alternate gene names: 89898167

Gene position: 421169-422824 (Clockwise)

Preceding gene: 89898166

Following gene: 89898170

Centisome position: 36.11

GC content: 39.31

Gene sequence:

>1656_bases
ATGCCTTCGCGAGTTCTGATTACCTCGGCATTACCTTATGCTAATGGACCTTTACATTTTGGACATATAGCCGGGGCCTA
TCTACCCGCAGATGTCTACGCTAGATTTCGTAGGTTATTAGGGGACGACGTCCTTTATATTTGTGGTTCTGATGAGTATG
GAATCGCAATCACACTAAATGCCGAACGCGCAGGTTTAGGCTACCAAGAGTATGTGAATATGTATCATAAGATACATAAA
GATACTTTTGATAAATTGAAGATTTCCCTCGATTTCTTCTCACGTACAACAAATCCTTTTCATAAAGCCCTTGTTGAGGA
TTTTTATACGCAGCTGAAGTCCAAAGGTCTTATAGAGAATCAGACTTCTCTTCAGTTATACTCTGAAGAAGAAAACCGTT
TTCTCGCAGATCGTTATGTAGAAGGTACGTGCCCTAGATGTGGGTTTGATGGAGCTCGTGGTGATGAGTGTCAAAAATGT
GGGGCTGACTATGAAGCTACTGACCTAATACATCCACGATCTAAATTGTCAGGATCCGAACTCGTTCTGAAAGAGACAGA
ACATGCCTATTTTCACTTAGAACGCATGGTCGAACCTTTATTGGATTTCATAGACAAGAGCTATTTACCAGAGCATGTCC
GAAAATTTATTGTAGATTATATAAAGAATTTACGCCCACGCGCCATTACCAGAGACCTATCTTGGGGAATTCCCGTTCCT
GATTTTCCTAATAAGGTTTTTTATGTCTGGTTTGATGCCCCTATTGGTTATATTAGTGGGACTATGGATTGGGCGGCATC
TGTAAATAATCCTGAAGCTTGGAAAGACTTTTGGTTGGAAGAGTCTACAGAGTATGTACAGTTTATAGGGAAGGATAATA
TTCCTTTTCATGCTGCGATATTTCCCGCAATGGAACTGGGACAGAGTATTCCTTATAAAAAGATGGATGCCCTAATATCT
TCTGAATTTTATCTTCTTGAAGGAGCACAATTTAGTAAATCCGAAGGGAATTACATAGATATGGATGCCTTTTTGGATAC
CTATTCTCTTGATAAATTACGTTACGTGTTGGCGGCAACAGCTCCAGAAACTTCTGATAGCGAGTTTACCTTCTTAGATT
TTAAAACACGCTGTAATTCGGAACTTGTGGGTAAATTCGGGAATTTTATTAATCGTGTTCTTGCTTTTGCTGAGAAAAAT
GGATTTAAGGAATTAGTATATTCCGAGACACTTTTAGAAGATAAAGATAAAAAGTTCCTAGATGATGCGCAAAGAATCGT
TCAGGAAGCTCGGGAGCATTATAGTAAGTATAGTTTGCGCAAGGCGTGTTCTGCGATTATGGAGTTAGCGGCATTAGGAA
ATATATACGTTAATGATCAGGCTCCATGGAAATTGCTTAAAGAGGGATTAGCCCATCGTGTAGAGGCGGTACTCTTTTGT
GCATGCTACTGTCAGAAATTGCTGGCGTTGATTTCTTATCCTATTCTTCCTGGAACAGCTTGGGAAATATGGAATATGTT
ATCTCCAAAATCTTTGGATCTTGTAAATCAAGACAAAGATCGAGTGAAAAACCTATGGAATTCGGAATTTTTCGATGTTT
CTGATGAGGTTTTTTCACTAAAAACGCCACAGCTGTTATGTGCGATTGTAGATTAG

Upstream 100 bases:

>100_bases
TAGAAAATATCTCCGCTCCCGTTACTGAAAGAAAAAGAGAAAGTTCTACTTCAGGAAGAAGAAAAGATCCTTCAGCTTAT
ACATGGAGTGATGTGAAATA

Downstream 100 bases:

>100_bases
GCTTTCTTATTCGTGAAAATGGGCAAGCTTGATATTGGGTTGGGTAAGTTGCTCAAGAGCTTGCCCAAGTCCTGTGCAGC
GGTGTTGTACCTTATCGTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MPSRVLITSALPYANGPLHFGHIAGAYLPADVYARFRRLLGDDVLYICGSDEYGIAITLNAERAGLGYQEYVNMYHKIHK
DTFDKLKISLDFFSRTTNPFHKALVEDFYTQLKSKGLIENQTSLQLYSEEENRFLADRYVEGTCPRCGFDGARGDECQKC
GADYEATDLIHPRSKLSGSELVLKETEHAYFHLERMVEPLLDFIDKSYLPEHVRKFIVDYIKNLRPRAITRDLSWGIPVP
DFPNKVFYVWFDAPIGYISGTMDWAASVNNPEAWKDFWLEESTEYVQFIGKDNIPFHAAIFPAMELGQSIPYKKMDALIS
SEFYLLEGAQFSKSEGNYIDMDAFLDTYSLDKLRYVLAATAPETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKN
GFKELVYSETLLEDKDKKFLDDAQRIVQEAREHYSKYSLRKACSAIMELAALGNIYVNDQAPWKLLKEGLAHRVEAVLFC
ACYCQKLLALISYPILPGTAWEIWNMLSPKSLDLVNQDKDRVKNLWNSEFFDVSDEVFSLKTPQLLCAIVD

Sequences:

>Translated_551_residues
MPSRVLITSALPYANGPLHFGHIAGAYLPADVYARFRRLLGDDVLYICGSDEYGIAITLNAERAGLGYQEYVNMYHKIHK
DTFDKLKISLDFFSRTTNPFHKALVEDFYTQLKSKGLIENQTSLQLYSEEENRFLADRYVEGTCPRCGFDGARGDECQKC
GADYEATDLIHPRSKLSGSELVLKETEHAYFHLERMVEPLLDFIDKSYLPEHVRKFIVDYIKNLRPRAITRDLSWGIPVP
DFPNKVFYVWFDAPIGYISGTMDWAASVNNPEAWKDFWLEESTEYVQFIGKDNIPFHAAIFPAMELGQSIPYKKMDALIS
SEFYLLEGAQFSKSEGNYIDMDAFLDTYSLDKLRYVLAATAPETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKN
GFKELVYSETLLEDKDKKFLDDAQRIVQEAREHYSKYSLRKACSAIMELAALGNIYVNDQAPWKLLKEGLAHRVEAVLFC
ACYCQKLLALISYPILPGTAWEIWNMLSPKSLDLVNQDKDRVKNLWNSEFFDVSDEVFSLKTPQLLCAIVD
>Mature_550_residues
PSRVLITSALPYANGPLHFGHIAGAYLPADVYARFRRLLGDDVLYICGSDEYGIAITLNAERAGLGYQEYVNMYHKIHKD
TFDKLKISLDFFSRTTNPFHKALVEDFYTQLKSKGLIENQTSLQLYSEEENRFLADRYVEGTCPRCGFDGARGDECQKCG
ADYEATDLIHPRSKLSGSELVLKETEHAYFHLERMVEPLLDFIDKSYLPEHVRKFIVDYIKNLRPRAITRDLSWGIPVPD
FPNKVFYVWFDAPIGYISGTMDWAASVNNPEAWKDFWLEESTEYVQFIGKDNIPFHAAIFPAMELGQSIPYKKMDALISS
EFYLLEGAQFSKSEGNYIDMDAFLDTYSLDKLRYVLAATAPETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKNG
FKELVYSETLLEDKDKKFLDDAQRIVQEAREHYSKYSLRKACSAIMELAALGNIYVNDQAPWKLLKEGLAHRVEAVLFCA
CYCQKLLALISYPILPGTAWEIWNMLSPKSLDLVNQDKDRVKNLWNSEFFDVSDEVFSLKTPQLLCAIVD

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=518, Percent_Identity=37.6447876447876, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI24308436, Length=526, Percent_Identity=26.2357414448669, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1788432, Length=501, Percent_Identity=34.3313373253493, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1786861, Length=147, Percent_Identity=31.2925170068027, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=512, Percent_Identity=35.3515625, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI212645096, Length=521, Percent_Identity=26.4875239923225, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71992781, Length=342, Percent_Identity=27.1929824561404, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=528, Percent_Identity=34.8484848484849, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6321610, Length=567, Percent_Identity=23.6331569664903, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24655311, Length=520, Percent_Identity=36.7307692307692, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI21358483, Length=523, Percent_Identity=24.4741873804971, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CHLFF (Q255A6)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515277.1
- ProteinModelPortal:   Q255A6
- SMR:   Q255A6
- STRING:   Q255A6
- GeneID:   3958343
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0360
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   ERNIMIC
- ProtClustDB:   PRK12268
- BioCyc:   CFEL264202:CF0360-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 63126; Mature: 62995

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRVLITSALPYANGPLHFGHIAGAYLPADVYARFRRLLGDDVLYICGSDEYGIAITLN
CCCCEEEEECCCCCCCCEEHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEE
AERAGLGYQEYVNMYHKIHKDTFDKLKISLDFFSRTTNPFHKALVEDFYTQLKSKGLIEN
CHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
QTSLQLYSEEENRFLADRYVEGTCPRCGFDGARGDECQKCGADYEATDLIHPRSKLSGSE
CCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCCHHHCCCCE
LVLKETEHAYFHLERMVEPLLDFIDKSYLPEHVRKFIVDYIKNLRPRAITRDLSWGIPVP
EEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
DFPNKVFYVWFDAPIGYISGTMDWAASVNNPEAWKDFWLEESTEYVQFIGKDNIPFHAAI
CCCCCEEEEEECCCHHHHCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHH
FPAMELGQSIPYKKMDALISSEFYLLEGAQFSKSEGNYIDMDAFLDTYSLDKLRYVLAAT
HHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC
APETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKNGFKELVYSETLLEDKDKKFL
CCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
DDAQRIVQEAREHYSKYSLRKACSAIMELAALGNIYVNDQAPWKLLKEGLAHRVEAVLFC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHH
ACYCQKLLALISYPILPGTAWEIWNMLSPKSLDLVNQDKDRVKNLWNSEFFDVSDEVFSL
HHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCC
KTPQLLCAIVD
CCCHHEEEECC
>Mature Secondary Structure 
PSRVLITSALPYANGPLHFGHIAGAYLPADVYARFRRLLGDDVLYICGSDEYGIAITLN
CCCEEEEECCCCCCCCEEHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEEEEEE
AERAGLGYQEYVNMYHKIHKDTFDKLKISLDFFSRTTNPFHKALVEDFYTQLKSKGLIEN
CHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
QTSLQLYSEEENRFLADRYVEGTCPRCGFDGARGDECQKCGADYEATDLIHPRSKLSGSE
CCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCCHHHCCCCE
LVLKETEHAYFHLERMVEPLLDFIDKSYLPEHVRKFIVDYIKNLRPRAITRDLSWGIPVP
EEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
DFPNKVFYVWFDAPIGYISGTMDWAASVNNPEAWKDFWLEESTEYVQFIGKDNIPFHAAI
CCCCCEEEEEECCCHHHHCCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHH
FPAMELGQSIPYKKMDALISSEFYLLEGAQFSKSEGNYIDMDAFLDTYSLDKLRYVLAAT
HHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC
APETSDSEFTFLDFKTRCNSELVGKFGNFINRVLAFAEKNGFKELVYSETLLEDKDKKFL
CCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHH
DDAQRIVQEAREHYSKYSLRKACSAIMELAALGNIYVNDQAPWKLLKEGLAHRVEAVLFC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHH
ACYCQKLLALISYPILPGTAWEIWNMLSPKSLDLVNQDKDRVKNLWNSEFFDVSDEVFSL
HHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCC
KTPQLLCAIVD
CCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA