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The map label for this gene is trmD

Identifier: 89898162

GI number: 89898162

Start: 418107

End: 419174

Strand: Direct

Name: trmD

Synonym: CF0355

Alternate gene names: 89898162

Gene position: 418107-419174 (Clockwise)

Preceding gene: 89898161

Following gene: 89898163

Centisome position: 35.85

GC content: 40.45

Gene sequence:

>1068_bases
ATGGAGATCGATATACTTTCTTTATTCCCAGATTATTTTGATAGCCCTTTGCGATCCAGTATTTTGGGTAGGGCTATTAA
GAACGGGCTGTTGAAAATCCAATCCAGAGATATACGAGAATTTGGGTTGGGGAAATGGAAACAGGTAGATGATGCCCCTT
TTAATAATGATGGAATGCTTCTTATGGCAGAGCCTGTGGTGCAGGCAATAAGATATGTTAAAAGAAGAGAATCCAGGGTA
ATTCATCTTTCACCACAAGGAGTGCCTTTAACAGCTCAAAAAAGCCGAGAATTAGCAAAGTGCTCACATTTGATATTCTT
GTGTGGACACTACGAAGGCATTGATGAAAGAGCCTTAGAAATTGAAGTGGATGAAGAGATAAGTATCGGTGATTATGTAT
TAACTAATGGGGGAATTGCTGCCTTAGTTGTAATCGATGCCCTATCTCGTTTTATTCCCGGAGTTTTGGGAAATCAGGAG
AGCGCAGAAAAGGATTCTCTAGAAAATGGGTTGTTAGAAGGACCTCAATATACACGTCCTCGAGTGTTTGAAGGTAGAGA
GGTTCCTCAAGTGCTTCTTCAAGGAGATCATCAAGCAATTGCTCGATGGAGAAAGCAAGTAAGTTTAGATAGAACCCGGG
AAAGACGGCCTGATTTATATGTTCGCTATCTTTATGATCGAGAGAGTGAGGATGTTCCCCAGATGGAAGCCGATCCAAAG
CAGTCTGCCTTTGAAGGAGAGTGTGCTGTAGTTTTGGATGTTGAAAACATAAATCGGTCAAAAAGATTTTATTCTAAGGT
ATTTAAATCAAATCAGCCTATTGGTGATAAGCTATACATCCCAGGGAAAACGCAGATGGTACTACATTTGCAAGAAGTGG
GGTTAAAAAATAAAAATACAGCCGTTTTGTCGCTTCGACTAGATTGTGAAAACGATTTTTATAGTTTTTTAGGACGATGG
AAAATGCTTGGAGGGACTCTAGAACAAGCTGACGATCGTGGAGCGGTGAGATTAGTTCGTGATTTTGACGGCCATTTATG
GGCAATCTCTTGCAAAAAGGCAAAATAG

Upstream 100 bases:

>100_bases
GCTGTTATGTGCCAAAAACGCCGTGCTTATCGCCAACGTCGTTCTTTAAAAAGAGCTGAAACCGCACAAGCTGTTTCTAA
ATAATTTTTAGGATTATGGG

Downstream 100 bases:

>100_bases
AAATAGAGAATTTAGGTGAAGTATATGGGGAACTTAATTAAGGAATTGCAAGACGAACAACTTCGAAAAGAAGATTTGGA
AGATTTTCGTGTTGGAGACA

Product: tRNA (guanine-N(1)-)-methyltransferase/unknown domain fusion protein

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MEIDILSLFPDYFDSPLRSSILGRAIKNGLLKIQSRDIREFGLGKWKQVDDAPFNNDGMLLMAEPVVQAIRYVKRRESRV
IHLSPQGVPLTAQKSRELAKCSHLIFLCGHYEGIDERALEIEVDEEISIGDYVLTNGGIAALVVIDALSRFIPGVLGNQE
SAEKDSLENGLLEGPQYTRPRVFEGREVPQVLLQGDHQAIARWRKQVSLDRTRERRPDLYVRYLYDRESEDVPQMEADPK
QSAFEGECAVVLDVENINRSKRFYSKVFKSNQPIGDKLYIPGKTQMVLHLQEVGLKNKNTAVLSLRLDCENDFYSFLGRW
KMLGGTLEQADDRGAVRLVRDFDGHLWAISCKKAK

Sequences:

>Translated_355_residues
MEIDILSLFPDYFDSPLRSSILGRAIKNGLLKIQSRDIREFGLGKWKQVDDAPFNNDGMLLMAEPVVQAIRYVKRRESRV
IHLSPQGVPLTAQKSRELAKCSHLIFLCGHYEGIDERALEIEVDEEISIGDYVLTNGGIAALVVIDALSRFIPGVLGNQE
SAEKDSLENGLLEGPQYTRPRVFEGREVPQVLLQGDHQAIARWRKQVSLDRTRERRPDLYVRYLYDRESEDVPQMEADPK
QSAFEGECAVVLDVENINRSKRFYSKVFKSNQPIGDKLYIPGKTQMVLHLQEVGLKNKNTAVLSLRLDCENDFYSFLGRW
KMLGGTLEQADDRGAVRLVRDFDGHLWAISCKKAK
>Mature_355_residues
MEIDILSLFPDYFDSPLRSSILGRAIKNGLLKIQSRDIREFGLGKWKQVDDAPFNNDGMLLMAEPVVQAIRYVKRRESRV
IHLSPQGVPLTAQKSRELAKCSHLIFLCGHYEGIDERALEIEVDEEISIGDYVLTNGGIAALVVIDALSRFIPGVLGNQE
SAEKDSLENGLLEGPQYTRPRVFEGREVPQVLLQGDHQAIARWRKQVSLDRTRERRPDLYVRYLYDRESEDVPQMEADPK
QSAFEGECAVVLDVENINRSKRFYSKVFKSNQPIGDKLYIPGKTQMVLHLQEVGLKNKNTAVLSLRLDCENDFYSFLGRW
KMLGGTLEQADDRGAVRLVRDFDGHLWAISCKKAK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=223, Percent_Identity=50.2242152466368, Blast_Score=207, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CHLFF (Q255B1)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515272.1
- ProteinModelPortal:   Q255B1
- SMR:   Q255B1
- STRING:   Q255B1
- GeneID:   3958574
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0355
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK01037
- BioCyc:   CFEL264202:CF0355-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 40381; Mature: 40381

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIDILSLFPDYFDSPLRSSILGRAIKNGLLKIQSRDIREFGLGKWKQVDDAPFNNDGML
CCEEEEECCHHHHCCHHHHHHHHHHHHHCCEEECCCCHHHHCCCCCCCCCCCCCCCCCEE
LMAEPVVQAIRYVKRRESRVIHLSPQGVPLTAQKSRELAKCSHLIFLCGHYEGIDERALE
EEEHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCEEE
IEVDEEISIGDYVLTNGGIAALVVIDALSRFIPGVLGNQESAEKDSLENGLLEGPQYTRP
EEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
RVFEGREVPQVLLQGDHQAIARWRKQVSLDRTRERRPDLYVRYLYDRESEDVPQMEADPK
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
QSAFEGECAVVLDVENINRSKRFYSKVFKSNQPIGDKLYIPGKTQMVLHLQEVGLKNKNT
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEHHHCCCCCCE
AVLSLRLDCENDFYSFLGRWKMLGGTLEQADDRGAVRLVRDFDGHLWAISCKKAK
EEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MEIDILSLFPDYFDSPLRSSILGRAIKNGLLKIQSRDIREFGLGKWKQVDDAPFNNDGML
CCEEEEECCHHHHCCHHHHHHHHHHHHHCCEEECCCCHHHHCCCCCCCCCCCCCCCCCEE
LMAEPVVQAIRYVKRRESRVIHLSPQGVPLTAQKSRELAKCSHLIFLCGHYEGIDERALE
EEEHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCEEE
IEVDEEISIGDYVLTNGGIAALVVIDALSRFIPGVLGNQESAEKDSLENGLLEGPQYTRP
EEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
RVFEGREVPQVLLQGDHQAIARWRKQVSLDRTRERRPDLYVRYLYDRESEDVPQMEADPK
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
QSAFEGECAVVLDVENINRSKRFYSKVFKSNQPIGDKLYIPGKTQMVLHLQEVGLKNKNT
CCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEHHHCCCCCCE
AVLSLRLDCENDFYSFLGRWKMLGGTLEQADDRGAVRLVRDFDGHLWAISCKKAK
EEEEEEECCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA