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The map label for this gene is pyk [H]

Identifier: 89898143

GI number: 89898143

Start: 394233

End: 395678

Strand: Direct

Name: pyk [H]

Synonym: CF0336

Alternate gene names: 89898143

Gene position: 394233-395678 (Clockwise)

Preceding gene: 161611313

Following gene: 89898148

Centisome position: 33.8

GC content: 40.46

Gene sequence:

>1446_bases
ATGATCGCAAGAACGAAAATCATTTGTACTATAGGTCCAGCAACAAATACCCCAGAAATGCTAGAGAAGCTCTTAGACGC
AGGAATGAATGTTGCTAGGTTAAATTTTAGCCATGGTACTCATGAAAGTCATGGTCAGACAATCCGTCTCCTTAAAGAAT
TGCGAGAAAGTAAGGGTGCTCCTTTAGCCATCATGTTAGATACTAAGGGACCCGAAGTTCGCTTGGGAAAGGTTCCTACA
CCAATCAAGGTATCTCGGGGTCAGAAAATCACCTTAGTAGGGAAAGATATCGAAGGGTCTATGGAAGAGGGAATTACTTT
ACATCCTCGGTGTGTGTTTCCTTTTGTGCGTGAAGGTGCAGAAGTCCTGATAGATGATGGATATATTCAAGCGATTGTGG
TTTCTACCGGTGATGATCATCTGGAATTAGAATTTATTAATTCCGGAGAGTTAAAATCCAATAAGTCTTTGAGTATCAGA
GAAATAGATCTTGCATTGCCATTCATGACGGATAAGGATATCGCTGATCTTAAGTTCGGGGTTGAACAAGGTGTTGATGT
TATTGCAGCTTCTTTCGTTCGCTGTGCAGAAGATATAGAAAGCATGCGTAAATGCCTAGCAGATTTTGGACGTGCAGATA
TGCCAATTATTGCTAAGATAGAAAATCGGTTGGGCGTGGAAAATTTCTCTAAGATTGCCCAGGCTTCAGACGGGATTATG
ATTGCCCGAGGGGATTTAGGAATTGAGCTTTCTGTTGTAGAAGTCCCCACTTTACAAAAGTTCATGGCAAGAGTGTCTCG
TGAAACTGGACGTTTTTGTATTACCGCTACGCAAATGTTAGAATCTATGATTCGCAATGTATTGCCAACTCGTGCTGAAG
TTTCGGATATTGCTAATGCTATTTATGACGGTACGTCTGCAGTAATGTTATCGGGAGAAACAGCTTCAGGAAGTTATCCT
ATAGCAGCCGTTAAAATTATGCGATCTGTGATTCAGGAGACAGAAAAAAATCTCAATTATAGCTCTTTCCTTAATCTTAA
TGATAGTGAATGTGCTGTTAAGGTATCTCCTTATCTTCAATCTATAGGCTTATCAGGAATTCAAATTGCAGAAAAAGCAG
AGGCTAGAGCTATTATTGTTTATACGGAATCTGGAGGATCTCCGATTTTTCTTTCCAAATACCGTCCTCGCTTCCCAATC
ATTGCTGTTACACCTAATCGAGATGTGTACTATAGGCTTGCCTTAGAGTGGGGTGTGTACCCTATGCTTACAGAGGAATC
CGTCCGTGCTGTATGGCGGCATCAAGCTTGTGTTTATGGTATCGAAAGGGGAATTTTATCAAATTACGATAAAGTTTTAG
TCCTGAGTCGTGGTGCGCAAATGAAAGATACAAATAATCTGACTTTGACAACAGTAAATGACATCATTACAGCATCAGCT
AAATAA

Upstream 100 bases:

>100_bases
TCACGTTTGATATTCTTAAGAGTCTATTGAATTATTTTTGTAAACGCGTGTTAGCTACAGAGTAAACAAGTTTTTGATTG
GTTTGAGAAGTTAGGGAAGT

Downstream 100 bases:

>100_bases
GCTGATGCTCTCTTACTTCTTATAAAAAGCGGTTTTTTGCTTTGAAACATCCTGCAGATAGCGAAACTGTAACAAGGGCC
ACGACCAGGAGTATCGGGGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGQTIRLLKELRESKGAPLAIMLDTKGPEVRLGKVPT
PIKVSRGQKITLVGKDIEGSMEEGITLHPRCVFPFVREGAEVLIDDGYIQAIVVSTGDDHLELEFINSGELKSNKSLSIR
EIDLALPFMTDKDIADLKFGVEQGVDVIAASFVRCAEDIESMRKCLADFGRADMPIIAKIENRLGVENFSKIAQASDGIM
IARGDLGIELSVVEVPTLQKFMARVSRETGRFCITATQMLESMIRNVLPTRAEVSDIANAIYDGTSAVMLSGETASGSYP
IAAVKIMRSVIQETEKNLNYSSFLNLNDSECAVKVSPYLQSIGLSGIQIAEKAEARAIIVYTESGGSPIFLSKYRPRFPI
IAVTPNRDVYYRLALEWGVYPMLTEESVRAVWRHQACVYGIERGILSNYDKVLVLSRGAQMKDTNNLTLTTVNDIITASA
K

Sequences:

>Translated_481_residues
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGQTIRLLKELRESKGAPLAIMLDTKGPEVRLGKVPT
PIKVSRGQKITLVGKDIEGSMEEGITLHPRCVFPFVREGAEVLIDDGYIQAIVVSTGDDHLELEFINSGELKSNKSLSIR
EIDLALPFMTDKDIADLKFGVEQGVDVIAASFVRCAEDIESMRKCLADFGRADMPIIAKIENRLGVENFSKIAQASDGIM
IARGDLGIELSVVEVPTLQKFMARVSRETGRFCITATQMLESMIRNVLPTRAEVSDIANAIYDGTSAVMLSGETASGSYP
IAAVKIMRSVIQETEKNLNYSSFLNLNDSECAVKVSPYLQSIGLSGIQIAEKAEARAIIVYTESGGSPIFLSKYRPRFPI
IAVTPNRDVYYRLALEWGVYPMLTEESVRAVWRHQACVYGIERGILSNYDKVLVLSRGAQMKDTNNLTLTTVNDIITASA
K
>Mature_481_residues
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGQTIRLLKELRESKGAPLAIMLDTKGPEVRLGKVPT
PIKVSRGQKITLVGKDIEGSMEEGITLHPRCVFPFVREGAEVLIDDGYIQAIVVSTGDDHLELEFINSGELKSNKSLSIR
EIDLALPFMTDKDIADLKFGVEQGVDVIAASFVRCAEDIESMRKCLADFGRADMPIIAKIENRLGVENFSKIAQASDGIM
IARGDLGIELSVVEVPTLQKFMARVSRETGRFCITATQMLESMIRNVLPTRAEVSDIANAIYDGTSAVMLSGETASGSYP
IAAVKIMRSVIQETEKNLNYSSFLNLNDSECAVKVSPYLQSIGLSGIQIAEKAEARAIIVYTESGGSPIFLSKYRPRFPI
IAVTPNRDVYYRLALEWGVYPMLTEESVRAVWRHQACVYGIERGILSNYDKVLVLSRGAQMKDTNNLTLTTVNDIITASA
K

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=475, Percent_Identity=40.8421052631579, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286420, Length=475, Percent_Identity=40.8421052631579, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286418, Length=471, Percent_Identity=40.552016985138, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI10835121, Length=465, Percent_Identity=39.7849462365591, Blast_Score=299, Evalue=3e-81,
Organism=Homo sapiens, GI32967597, Length=465, Percent_Identity=39.7849462365591, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=43.006993006993, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=43.006993006993, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI310128736, Length=234, Percent_Identity=42.3076923076923, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI310128734, Length=234, Percent_Identity=42.3076923076923, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=40.9302325581395, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1787965, Length=434, Percent_Identity=42.6267281105991, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1788160, Length=471, Percent_Identity=37.1549893842887, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=38.3983572895277, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17506829, Length=489, Percent_Identity=38.4458077709611, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=489, Percent_Identity=38.4458077709611, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=489, Percent_Identity=38.6503067484663, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=489, Percent_Identity=38.4458077709611, Blast_Score=269, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=484, Percent_Identity=39.2561983471074, Blast_Score=293, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=37.1900826446281, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=39.5061728395062, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=39.5061728395062, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=39.9515738498789, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24581235, Length=426, Percent_Identity=32.3943661971831, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24646914, Length=261, Percent_Identity=36.7816091954023, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52797; Mature: 52797

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGQTIRLLKELRESKGA
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCC
PLAIMLDTKGPEVRLGKVPTPIKVSRGQKITLVGKDIEGSMEEGITLHPRCVFPFVREGA
CEEEEEECCCCCEEECCCCCCEEECCCCEEEEEECCCCCHHHCCCEECCCEECHHHHCCC
EVLIDDGYIQAIVVSTGDDHLELEFINSGELKSNKSLSIREIDLALPFMTDKDIADLKFG
EEEEECCEEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHC
VEQGVDVIAASFVRCAEDIESMRKCLADFGRADMPIIAKIENRLGVENFSKIAQASDGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCHHHHHHHHHCCCCEE
IARGDLGIELSVVEVPTLQKFMARVSRETGRFCITATQMLESMIRNVLPTRAEVSDIANA
EEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHH
IYDGTSAVMLSGETASGSYPIAAVKIMRSVIQETEKNLNYSSFLNLNDSECAVKVSPYLQ
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEECCCCCCEEEEECHHHH
SIGLSGIQIAEKAEARAIIVYTESGGSPIFLSKYRPRFPIIAVTPNRDVYYRLALEWGVY
HCCCCCEEEHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCEEEEEEEECCCC
PMLTEESVRAVWRHQACVYGIERGILSNYDKVLVLSRGAQMKDTNNLTLTTVNDIITASA
EEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEHHEEEECC
K
C
>Mature Secondary Structure
MIARTKIICTIGPATNTPEMLEKLLDAGMNVARLNFSHGTHESHGQTIRLLKELRESKGA
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCC
PLAIMLDTKGPEVRLGKVPTPIKVSRGQKITLVGKDIEGSMEEGITLHPRCVFPFVREGA
CEEEEEECCCCCEEECCCCCCEEECCCCEEEEEECCCCCHHHCCCEECCCEECHHHHCCC
EVLIDDGYIQAIVVSTGDDHLELEFINSGELKSNKSLSIREIDLALPFMTDKDIADLKFG
EEEEECCEEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHC
VEQGVDVIAASFVRCAEDIESMRKCLADFGRADMPIIAKIENRLGVENFSKIAQASDGIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCHHHHHHHHHCCCCEE
IARGDLGIELSVVEVPTLQKFMARVSRETGRFCITATQMLESMIRNVLPTRAEVSDIANA
EEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHH
IYDGTSAVMLSGETASGSYPIAAVKIMRSVIQETEKNLNYSSFLNLNDSECAVKVSPYLQ
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHEECCCCCCEEEEECHHHH
SIGLSGIQIAEKAEARAIIVYTESGGSPIFLSKYRPRFPIIAVTPNRDVYYRLALEWGVY
HCCCCCEEEHHCCCCCEEEEEECCCCCEEEEECCCCCCCEEEECCCCCEEEEEEEECCCC
PMLTEESVRAVWRHQACVYGIERGILSNYDKVLVLSRGAQMKDTNNLTLTTVNDIITASA
EEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEHHEEEECC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]