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The map label for this gene is mdhC [H]

Identifier: 89898089

GI number: 89898089

Start: 329140

End: 330126

Strand: Direct

Name: mdhC [H]

Synonym: CF0282

Alternate gene names: 89898089

Gene position: 329140-330126 (Clockwise)

Preceding gene: 89898088

Following gene: 89898090

Centisome position: 28.22

GC content: 42.65

Gene sequence:

>987_bases
ATGAAACTAGCACGTACGGTTAGCGTTGCTGTGACGGGGGGAACAGGGCAAATTGCCTACAGTTTTTTGTTCGCTCTAGC
TCATGGGGATGTTTTTGGTAGTGATTGTGGAATAGATTTACGTGTGTACGATCTCCCGGGCCTAGAAAGGGTGCTTTCCG
GGATTCGTATGGAGCTTGATGATTGCGCTTATCCTCTTTTACAATCCCTGCGTGTTACCACCTCCTTAGAAGATGCTTTT
GATGGTATTGATGCTGCCTTTCTTATAGGGGCGGCTCCTCGAGGTCCTGGAATGGAACGCTCTGATCTTTTAAAGCGTAA
TGGTGAGATTTTTTCTCTTCAGGGATCTGTTTTAAATACTTCTGCAAAGCGTGATGCTAAAATTTTTGTTGTTGGTAATC
CTGTAAATACCAACTGTTGGATAGCAATGAGTCGGGCTCCGAAATTAAATAGAAGAAACTTTCACTCCATGCTGCGTTTA
GATCAGAACCGCATGCAGGCAATGTTAGCACACCGAGCGCAAGTGCCTTTGGACGAAGTTACCAATGTGGTTATTTGGGG
CAATCATTCTGCAAAGCAAGTTCCTGATTTTACTCAGTCATTAATTTCTGGAAAACCTGCCGTGGAAGTTATTAGTGATC
GAGACTGGTTGGAAAACATTATGCTACCTTCTATTCAAAATCGAGGAAGTGCTGTTATTGAAGCTCGTGGAAAATCTTCG
GCAGGATCTGCTGCTCGTGCTTTAGCAGAGGCCGCACGTTCTATTTTTGTCCCTAAAGAGGGTGAGTGGTTCTCTACTGG
AGTATGTTCCGACTACAATCCCTATGGTATTCCTGAAGATCTAATTTTTGGTTTCCCATGTCGTATGTTGCCATCTGGGG
ATTACGAAATTATTCCTGGATTGTCTTGGGATGTCTTTATAAAAAATAAGATTCAAATATCCTTAGATGAAATTTCTCAG
GAAAAAGCCAATGTCTCTTTGTTATAG

Upstream 100 bases:

>100_bases
TGTGCCTGAGGAGTTTTCTTTCTTTAGTTATCGAAAATTAGACGCGCTTGATTTTGTTTTTATTTAAAAATTATACTGAT
TTTTTGAGAGAGGCGTGATT

Downstream 100 bases:

>100_bases
CGCCAGTTAGTGGGGTAATTTATGACTCAATCTTTTGATGTATTAAGTCAAAATGCTTTTAAAAAGATTTTTAATAAACA
GCGGTTTCTTTTTATTTTTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKLARTVSVAVTGGTGQIAYSFLFALAHGDVFGSDCGIDLRVYDLPGLERVLSGIRMELDDCAYPLLQSLRVTTSLEDAF
DGIDAAFLIGAAPRGPGMERSDLLKRNGEIFSLQGSVLNTSAKRDAKIFVVGNPVNTNCWIAMSRAPKLNRRNFHSMLRL
DQNRMQAMLAHRAQVPLDEVTNVVIWGNHSAKQVPDFTQSLISGKPAVEVISDRDWLENIMLPSIQNRGSAVIEARGKSS
AGSAARALAEAARSIFVPKEGEWFSTGVCSDYNPYGIPEDLIFGFPCRMLPSGDYEIIPGLSWDVFIKNKIQISLDEISQ
EKANVSLL

Sequences:

>Translated_328_residues
MKLARTVSVAVTGGTGQIAYSFLFALAHGDVFGSDCGIDLRVYDLPGLERVLSGIRMELDDCAYPLLQSLRVTTSLEDAF
DGIDAAFLIGAAPRGPGMERSDLLKRNGEIFSLQGSVLNTSAKRDAKIFVVGNPVNTNCWIAMSRAPKLNRRNFHSMLRL
DQNRMQAMLAHRAQVPLDEVTNVVIWGNHSAKQVPDFTQSLISGKPAVEVISDRDWLENIMLPSIQNRGSAVIEARGKSS
AGSAARALAEAARSIFVPKEGEWFSTGVCSDYNPYGIPEDLIFGFPCRMLPSGDYEIIPGLSWDVFIKNKIQISLDEISQ
EKANVSLL
>Mature_328_residues
MKLARTVSVAVTGGTGQIAYSFLFALAHGDVFGSDCGIDLRVYDLPGLERVLSGIRMELDDCAYPLLQSLRVTTSLEDAF
DGIDAAFLIGAAPRGPGMERSDLLKRNGEIFSLQGSVLNTSAKRDAKIFVVGNPVNTNCWIAMSRAPKLNRRNFHSMLRL
DQNRMQAMLAHRAQVPLDEVTNVVIWGNHSAKQVPDFTQSLISGKPAVEVISDRDWLENIMLPSIQNRGSAVIEARGKSS
AGSAARALAEAARSIFVPKEGEWFSTGVCSDYNPYGIPEDLIFGFPCRMLPSGDYEIIPGLSWDVFIKNKIQISLDEISQ
EKANVSLL

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=325, Percent_Identity=42.4615384615385, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI89886456, Length=330, Percent_Identity=22.7272727272727, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561064, Length=320, Percent_Identity=42.5, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI32566798, Length=233, Percent_Identity=43.3476394849785, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI32566800, Length=171, Percent_Identity=38.5964912280702, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=43.0769230769231, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35865; Mature: 35865

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLARTVSVAVTGGTGQIAYSFLFALAHGDVFGSDCGIDLRVYDLPGLERVLSGIRMELD
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
DCAYPLLQSLRVTTSLEDAFDGIDAAFLIGAAPRGPGMERSDLLKRNGEIFSLQGSVLNT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEEEECCEECC
SAKRDAKIFVVGNPVNTNCWIAMSRAPKLNRRNFHSMLRLDQNRMQAMLAHRAQVPLDEV
CCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TNVVIWGNHSAKQVPDFTQSLISGKPAVEVISDRDWLENIMLPSIQNRGSAVIEARGKSS
CEEEEECCCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCCCEEEEECCCCC
AGSAARALAEAARSIFVPKEGEWFSTGVCSDYNPYGIPEDLIFGFPCRMLPSGDYEIIPG
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCCEECCCCCEEEECC
LSWDVFIKNKIQISLDEISQEKANVSLL
CEEEEEEECEEEEEHHHHHHHCCCEECC
>Mature Secondary Structure
MKLARTVSVAVTGGTGQIAYSFLFALAHGDVFGSDCGIDLRVYDLPGLERVLSGIRMELD
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
DCAYPLLQSLRVTTSLEDAFDGIDAAFLIGAAPRGPGMERSDLLKRNGEIFSLQGSVLNT
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEEEECCEECC
SAKRDAKIFVVGNPVNTNCWIAMSRAPKLNRRNFHSMLRLDQNRMQAMLAHRAQVPLDEV
CCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
TNVVIWGNHSAKQVPDFTQSLISGKPAVEVISDRDWLENIMLPSIQNRGSAVIEARGKSS
CEEEEECCCCCCCCCHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCCCEEEEECCCCC
AGSAARALAEAARSIFVPKEGEWFSTGVCSDYNPYGIPEDLIFGFPCRMLPSGDYEIIPG
CCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCHHHHCCCCCEECCCCCEEEECC
LSWDVFIKNKIQISLDEISQEKANVSLL
CEEEEEEECEEEEEHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA