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The map label for this gene is lysS

Identifier: 89897981

GI number: 89897981

Start: 206366

End: 207946

Strand: Direct

Name: lysS

Synonym: CF0174

Alternate gene names: 89897981

Gene position: 206366-207946 (Clockwise)

Preceding gene: 89897980

Following gene: 89897984

Centisome position: 17.7

GC content: 38.77

Gene sequence:

>1581_bases
ATGTCTGCAAAAGCTGAGTACCTACAACAAGAAGATTTTCTCCATAGAAGTAATAAGCTCCAGGAAATATCTGACCTAGG
TATCAATCCCTATCCTTACGAATTCCCTGGAACGAGTAGTGTTGAGGAAATTAAAAACGAATTTTCTTCTCAGTCTCTTG
GAAATAGCGAAGATGCTACGAATAAAAATACCCCCAAGGTGAAAATCTCAGGTCGTATGGTGCTTTTCCGTTCCATGGGG
AAAAATGCTTTTGCTCAGATTTTAGATAATGACCAAAAGATTCAGGTAATGTTTAACAGGGATTTTTCCTCAGTTGCAGG
TCTCCCTGAAGATGCTGAGATCACACCTATAAAATTTATAGAAAAGAAACTGGATCTCGGTGACATTCTAGGAATCGAAG
GGTACCTATTCTTCACACATTCCGGAGAACTCACCATCTTGGTAGAAACTGTCACGTTATTGGGTAAAGCATTAATCTCA
CTTCCTGATAAACATGCAGGATTGAGTGATAAAGAAACTCGTTATAGAAAACGTTGGTTAGATCTGATTTGTTCTGATGA
GGTGCGTCAAACTTTCTTAAAAAGAAGTCGTATTATCAAGTTAATCCGTCAGTATATGGATGCTCAGGATTTCATAGAGG
TAGAAACTCCTATATTGCAAAATATCTATGGTGGGGCTGAAGCAACTCCTTTTGTAACTACTTTGAATGCTTTGCATTCT
GATATGTTTTTAAGAATTTCACTAGAGATTGCCTTAAAGAAAATCCTGGTTGGAGGGACCCCTCGTGTCTATGAAATTGG
TAAGGTGTTTAGGAATGAAGGGATAGACAGAACTCACAATCCTGAATTTACTATGATAGAAGCTTATGCAATGAATATAG
ACTATCATAGCGTCATGGTGTACGTAGAGAACCTTATTGAATACCTTGTTGGCGAATTAAATAATGGTAGTACGGTACTG
ACTTATTCACATCTCAAGCAAGGGCCTCAAACTATTGATTTTAAAGCTCCTTGGATTCGTATGACTATGAAAGATAGTAT
CAAAACATATGGAGGAGTTGATGTGGACCTTCATGGGGATCATGAATTGCGTAATATTTTAAAAGAGCGCTCCTCATTAC
CCGAAGAATCTTATGCAACAGCGCCTAGGGGTTTATTAATCGCGGCCCTATTTGATGAGTTAGTATGTGATAAGTTAATT
GCTCCCCATCATATTACGGATCATCCTTTAGAGACTACACCACTATGTAAGTCTTTGCGCTCTGGAGAAGAGGATTATGT
AGAGCGTTTTGAAAGTTTTTGTTTAGGTAAAGAACTCTGCAATGCTTATTCAGAACTGACTGATCCAATGCGTCAGAGAA
TGCTTTTGGAAAAGCAAATGGAGAAAAAAGCTTTAGATCCAGATAGCGAATATCACCCTATTGATGAGGAGTTTCTAGAA
GCCCTATGTCAGGGTATGCCTCCTGCAGGTGGATTCGGAATCGGTATCGATCGTTTGGTTATGATTCTGACAGATTCCGC
TTCTATTCGCGATGTTCTATATTTCCCAGTTATGCGTCGTCTAGAATCAGAAAATGACTAA

Upstream 100 bases:

>100_bases
TCCCTTCTTCAGACAGTATTTAAAATTTTTATAGCGGATCACATTATTAAAAATAGAACTGTGTTTGAAGAAGAAGAATT
AGTCAAAAGGATTTAAAAAA

Downstream 100 bases:

>100_bases
AGTTTTTTTACCTTAGGTCCTGATTTGGAATCCTCGATCAGGTAGCCTAAAGAGGCTACCTGATCTCGAAATGCATCAGC
TTGTGCCCAGTTTTTCTCTC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSAKAEYLQQEDFLHRSNKLQEISDLGINPYPYEFPGTSSVEEIKNEFSSQSLGNSEDATNKNTPKVKISGRMVLFRSMG
KNAFAQILDNDQKIQVMFNRDFSSVAGLPEDAEITPIKFIEKKLDLGDILGIEGYLFFTHSGELTILVETVTLLGKALIS
LPDKHAGLSDKETRYRKRWLDLICSDEVRQTFLKRSRIIKLIRQYMDAQDFIEVETPILQNIYGGAEATPFVTTLNALHS
DMFLRISLEIALKKILVGGTPRVYEIGKVFRNEGIDRTHNPEFTMIEAYAMNIDYHSVMVYVENLIEYLVGELNNGSTVL
TYSHLKQGPQTIDFKAPWIRMTMKDSIKTYGGVDVDLHGDHELRNILKERSSLPEESYATAPRGLLIAALFDELVCDKLI
APHHITDHPLETTPLCKSLRSGEEDYVERFESFCLGKELCNAYSELTDPMRQRMLLEKQMEKKALDPDSEYHPIDEEFLE
ALCQGMPPAGGFGIGIDRLVMILTDSASIRDVLYFPVMRRLESEND

Sequences:

>Translated_526_residues
MSAKAEYLQQEDFLHRSNKLQEISDLGINPYPYEFPGTSSVEEIKNEFSSQSLGNSEDATNKNTPKVKISGRMVLFRSMG
KNAFAQILDNDQKIQVMFNRDFSSVAGLPEDAEITPIKFIEKKLDLGDILGIEGYLFFTHSGELTILVETVTLLGKALIS
LPDKHAGLSDKETRYRKRWLDLICSDEVRQTFLKRSRIIKLIRQYMDAQDFIEVETPILQNIYGGAEATPFVTTLNALHS
DMFLRISLEIALKKILVGGTPRVYEIGKVFRNEGIDRTHNPEFTMIEAYAMNIDYHSVMVYVENLIEYLVGELNNGSTVL
TYSHLKQGPQTIDFKAPWIRMTMKDSIKTYGGVDVDLHGDHELRNILKERSSLPEESYATAPRGLLIAALFDELVCDKLI
APHHITDHPLETTPLCKSLRSGEEDYVERFESFCLGKELCNAYSELTDPMRQRMLLEKQMEKKALDPDSEYHPIDEEFLE
ALCQGMPPAGGFGIGIDRLVMILTDSASIRDVLYFPVMRRLESEND
>Mature_525_residues
SAKAEYLQQEDFLHRSNKLQEISDLGINPYPYEFPGTSSVEEIKNEFSSQSLGNSEDATNKNTPKVKISGRMVLFRSMGK
NAFAQILDNDQKIQVMFNRDFSSVAGLPEDAEITPIKFIEKKLDLGDILGIEGYLFFTHSGELTILVETVTLLGKALISL
PDKHAGLSDKETRYRKRWLDLICSDEVRQTFLKRSRIIKLIRQYMDAQDFIEVETPILQNIYGGAEATPFVTTLNALHSD
MFLRISLEIALKKILVGGTPRVYEIGKVFRNEGIDRTHNPEFTMIEAYAMNIDYHSVMVYVENLIEYLVGELNNGSTVLT
YSHLKQGPQTIDFKAPWIRMTMKDSIKTYGGVDVDLHGDHELRNILKERSSLPEESYATAPRGLLIAALFDELVCDKLIA
PHHITDHPLETTPLCKSLRSGEEDYVERFESFCLGKELCNAYSELTDPMRQRMLLEKQMEKKALDPDSEYHPIDEEFLEA
LCQGMPPAGGFGIGIDRLVMILTDSASIRDVLYFPVMRRLESEND

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=518, Percent_Identity=41.3127413127413, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI194272210, Length=518, Percent_Identity=41.3127413127413, Blast_Score=328, Evalue=7e-90,
Organism=Escherichia coli, GI1790571, Length=511, Percent_Identity=40.1174168297456, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1789256, Length=507, Percent_Identity=40.4339250493097, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI87082379, Length=334, Percent_Identity=27.5449101796407, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788173, Length=303, Percent_Identity=24.0924092409241, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=524, Percent_Identity=37.4045801526718, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17535927, Length=524, Percent_Identity=37.4045801526718, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI71994340, Length=507, Percent_Identity=38.0670611439842, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI32566633, Length=217, Percent_Identity=26.7281105990783, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=525, Percent_Identity=38.2857142857143, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6324256, Length=507, Percent_Identity=30.7692307692308, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=352, Percent_Identity=25.8522727272727, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=472, Percent_Identity=23.0932203389831, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24640849, Length=520, Percent_Identity=39.2307692307692, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24640851, Length=520, Percent_Identity=39.6153846153846, Blast_Score=319, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_CHLFF (Q255U2)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515091.1
- ProteinModelPortal:   Q255U2
- SMR:   Q255U2
- STRING:   Q255U2
- GeneID:   3958335
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0174
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   DEEAMIV
- ProtClustDB:   PRK00484
- BioCyc:   CFEL264202:CF0174-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 59763; Mature: 59632

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKAEYLQQEDFLHRSNKLQEISDLGINPYPYEFPGTSSVEEIKNEFSSQSLGNSEDAT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
NKNTPKVKISGRMVLFRSMGKNAFAQILDNDQKIQVMFNRDFSSVAGLPEDAEITPIKFI
CCCCCEEEECCCEEEEHHHCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCHHHHH
EKKLDLGDILGIEGYLFFTHSGELTILVETVTLLGKALISLPDKHAGLSDKETRYRKRWL
HHHCCHHHEECCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
DLICSDEVRQTFLKRSRIIKLIRQYMDAQDFIEVETPILQNIYGGAEATPFVTTLNALHS
HHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
DMFLRISLEIALKKILVGGTPRVYEIGKVFRNEGIDRTHNPEFTMIEAYAMNIDYHSVMV
CCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHHHH
YVENLIEYLVGELNNGSTVLTYSHLKQGPQTIDFKAPWIRMTMKDSIKTYGGVDVDLHGD
HHHHHHHHHHHCCCCCCEEEEEHHHHCCCCEECCCCCEEEEEHHHHHHHCCCEEEEECCC
HELRNILKERSSLPEESYATAPRGLLIAALFDELVCDKLIAPHHITDHPLETTPLCKSLR
HHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
SGEEDYVERFESFCLGKELCNAYSELTDPMRQRMLLEKQMEKKALDPDSEYHPIDEEFLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ALCQGMPPAGGFGIGIDRLVMILTDSASIRDVLYFPVMRRLESEND
HHHCCCCCCCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAKAEYLQQEDFLHRSNKLQEISDLGINPYPYEFPGTSSVEEIKNEFSSQSLGNSEDAT
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
NKNTPKVKISGRMVLFRSMGKNAFAQILDNDQKIQVMFNRDFSSVAGLPEDAEITPIKFI
CCCCCEEEECCCEEEEHHHCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCHHHHH
EKKLDLGDILGIEGYLFFTHSGELTILVETVTLLGKALISLPDKHAGLSDKETRYRKRWL
HHHCCHHHEECCCEEEEEEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
DLICSDEVRQTFLKRSRIIKLIRQYMDAQDFIEVETPILQNIYGGAEATPFVTTLNALHS
HHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
DMFLRISLEIALKKILVGGTPRVYEIGKVFRNEGIDRTHNPEFTMIEAYAMNIDYHSVMV
CCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCHHHHHH
YVENLIEYLVGELNNGSTVLTYSHLKQGPQTIDFKAPWIRMTMKDSIKTYGGVDVDLHGD
HHHHHHHHHHHCCCCCCEEEEEHHHHCCCCEECCCCCEEEEEHHHHHHHCCCEEEEECCC
HELRNILKERSSLPEESYATAPRGLLIAALFDELVCDKLIAPHHITDHPLETTPLCKSLR
HHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
SGEEDYVERFESFCLGKELCNAYSELTDPMRQRMLLEKQMEKKALDPDSEYHPIDEEFLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
ALCQGMPPAGGFGIGIDRLVMILTDSASIRDVLYFPVMRRLESEND
HHHCCCCCCCCCCCCHHHEEHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA