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The map label for this gene is miaA

Identifier: 89897965

GI number: 89897965

Start: 189468

End: 190496

Strand: Direct

Name: miaA

Synonym: CF0158

Alternate gene names: 89897965

Gene position: 189468-190496 (Clockwise)

Preceding gene: 89897964

Following gene: 89897967

Centisome position: 16.25

GC content: 41.21

Gene sequence:

>1029_bases
ATGCGTGCTCCTGAATTTCAAGCTAATGCAATAACATCTACAGGATGTGACGTCTGCTTAGATCCTCAAAAGTCATTTGC
GAAACTGTTTAAACGTACTATTATCTTACTCGCAGGGCCTACTGGATCTGGGAAGACCGATGTTTCTTTATGTCTAGCGC
CCATGGTTGATGGAGAAATTGTTTCCGTGGATTCCATGCAGGTTTATCGTGGGATGGACATCGGTACTGCTAAGGTTTCT
TTGGAAGCCAGACAGAGAATCCCTCACTATCTGATCGATATCTGTCATGTTCAGGAACTCTTTAATGCTGTAGATTTTTA
TTATCAGGCAACCCAAGCATGCCAAAATATCCTGTCTAGGAATAAGGTTCCTATTCTTGTCGGTGGGTCCGGGTTTTACT
TTCACACTTTTCTTTCAGGTCCTCCTGAAGGACCACCCGCAGATCGTGAATTTCGCGATCACCTAGCTCTTTACATTCAG
AAGAATGGCTTGTCTCTCCTATACGATAATCTATGTATGAAAGATCCTGAATATGCGCGTACTATTACCAAGAATGATAA
GAATAAAATCGTTCGTGCTCTAGAAATTATCCATTTAACAGGGAAAAAGGTCTCAGAGCATAACTGGAGCATGGAAGCAA
AAGAACCTAGGGAATACAATTGTCGAGGATGGTTCCTTTCTTCGCCTAGAGATCTTTTGCGCGATAATATTCAATTGCGT
TGCCGCAGAATGCTTGAGGATAACTTAATTGACGAAGTGCATAGATTGCTCGAGCAGGGAATTAGAGAGAATCCCTCAGC
ATCAAAGGCTATAGGCTATAGAGAATGGATTGATTTTATTGATCAAGGATCTCCCAAAGAAGCTTATGAGGACGTGAAGA
ATAAGTTCATTGCCAATACATTGTATTATATTAAAAAACAAAGAACTTGGTTTAAGCGCTATCCCATGTTTCGAGAACTG
CCTACCCTGGGGTTGACAGCAGAAACTATAGCAGAAAAGATCGCTGAAGATTACTTTCTTCATGGATGA

Upstream 100 bases:

>100_bases
ATGCAGTGGCTGAGGTTATTCGTATAGCGGGAGTCGATCAACTTTTGCCTGTATGTCAGTCAGAACAAGAATGTTTTACT
AAATTTTAACCATATTGCAT

Downstream 100 bases:

>100_bases
TGCGCAGGCGTTTCAATGCGCTCATAGAATGTATTTCTCTCTACAGGAATAAATCCTTCAGAAGCAATTATTGCTCGCGC
TTCTTCTTCAGAACTTTTTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRAPEFQANAITSTGCDVCLDPQKSFAKLFKRTIILLAGPTGSGKTDVSLCLAPMVDGEIVSVDSMQVYRGMDIGTAKVS
LEARQRIPHYLIDICHVQELFNAVDFYYQATQACQNILSRNKVPILVGGSGFYFHTFLSGPPEGPPADREFRDHLALYIQ
KNGLSLLYDNLCMKDPEYARTITKNDKNKIVRALEIIHLTGKKVSEHNWSMEAKEPREYNCRGWFLSSPRDLLRDNIQLR
CRRMLEDNLIDEVHRLLEQGIRENPSASKAIGYREWIDFIDQGSPKEAYEDVKNKFIANTLYYIKKQRTWFKRYPMFREL
PTLGLTAETIAEKIAEDYFLHG

Sequences:

>Translated_342_residues
MRAPEFQANAITSTGCDVCLDPQKSFAKLFKRTIILLAGPTGSGKTDVSLCLAPMVDGEIVSVDSMQVYRGMDIGTAKVS
LEARQRIPHYLIDICHVQELFNAVDFYYQATQACQNILSRNKVPILVGGSGFYFHTFLSGPPEGPPADREFRDHLALYIQ
KNGLSLLYDNLCMKDPEYARTITKNDKNKIVRALEIIHLTGKKVSEHNWSMEAKEPREYNCRGWFLSSPRDLLRDNIQLR
CRRMLEDNLIDEVHRLLEQGIRENPSASKAIGYREWIDFIDQGSPKEAYEDVKNKFIANTLYYIKKQRTWFKRYPMFREL
PTLGLTAETIAEKIAEDYFLHG
>Mature_342_residues
MRAPEFQANAITSTGCDVCLDPQKSFAKLFKRTIILLAGPTGSGKTDVSLCLAPMVDGEIVSVDSMQVYRGMDIGTAKVS
LEARQRIPHYLIDICHVQELFNAVDFYYQATQACQNILSRNKVPILVGGSGFYFHTFLSGPPEGPPADREFRDHLALYIQ
KNGLSLLYDNLCMKDPEYARTITKNDKNKIVRALEIIHLTGKKVSEHNWSMEAKEPREYNCRGWFLSSPRDLLRDNIQLR
CRRMLEDNLIDEVHRLLEQGIRENPSASKAIGYREWIDFIDQGSPKEAYEDVKNKFIANTLYYIKKQRTWFKRYPMFREL
PTLGLTAETIAEKIAEDYFLHG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=334, Percent_Identity=28.7425149700599, Blast_Score=106, Evalue=2e-23,
Organism=Escherichia coli, GI1790613, Length=282, Percent_Identity=30.8510638297872, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144712, Length=238, Percent_Identity=26.4705882352941, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=27.7397260273973, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24649830, Length=278, Percent_Identity=31.6546762589928, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLFF (Q255V8)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515075.1
- ProteinModelPortal:   Q255V8
- SMR:   Q255V8
- STRING:   Q255V8
- GeneID:   3958950
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0158
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   CFEL264202:CF0158-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 39228; Mature: 39228

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPEFQANAITSTGCDVCLDPQKSFAKLFKRTIILLAGPTGSGKTDVSLCLAPMVDGEI
CCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHCCCCCE
VSVDSMQVYRGMDIGTAKVSLEARQRIPHYLIDICHVQELFNAVDFYYQATQACQNILSR
EEECCHHHHCCCCCCHHEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKVPILVGGSGFYFHTFLSGPPEGPPADREFRDHLALYIQKNGLSLLYDNLCMKDPEYAR
CCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHCCCCHHHH
TITKNDKNKIVRALEIIHLTGKKVSEHNWSMEAKEPREYNCRGWFLSSPRDLLRDNIQLR
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
CRRMLEDNLIDEVHRLLEQGIRENPSASKAIGYREWIDFIDQGSPKEAYEDVKNKFIANT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LYYIKKQRTWFKRYPMFRELPTLGLTAETIAEKIAEDYFLHG
HHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRAPEFQANAITSTGCDVCLDPQKSFAKLFKRTIILLAGPTGSGKTDVSLCLAPMVDGEI
CCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHCCCCCE
VSVDSMQVYRGMDIGTAKVSLEARQRIPHYLIDICHVQELFNAVDFYYQATQACQNILSR
EEECCHHHHCCCCCCHHEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NKVPILVGGSGFYFHTFLSGPPEGPPADREFRDHLALYIQKNGLSLLYDNLCMKDPEYAR
CCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHCCCCHHHH
TITKNDKNKIVRALEIIHLTGKKVSEHNWSMEAKEPREYNCRGWFLSSPRDLLRDNIQLR
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
CRRMLEDNLIDEVHRLLEQGIRENPSASKAIGYREWIDFIDQGSPKEAYEDVKNKFIANT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LYYIKKQRTWFKRYPMFRELPTLGLTAETIAEKIAEDYFLHG
HHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA