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The map label for this gene is folA [H]

Identifier: 89897822

GI number: 89897822

Start: 14366

End: 14854

Strand: Direct

Name: folA [H]

Synonym: CF0015

Alternate gene names: 89897822

Gene position: 14366-14854 (Clockwise)

Preceding gene: 89897821

Following gene: 89897823

Centisome position: 1.23

GC content: 39.67

Gene sequence:

>489_bases
ATGTGTAAAATACTTGGTATTGTAGCTTGTAGTCCTAGTGGGGTGATTGGAAACCAAGGAAAGTTGCCTTGGAGCTATTC
TAAGGACATTGAATTTTTCGCAAAAACGATAGAAAACAAGCCCTTAGTTATGGGGAGAAAAACCTGGGAGGGTCTTCCTG
AAAAATATACGAAGGGCCGTCAGATTTTCGTGTTTTCTAGAAGTTTTTATGACAATGCCGAGGGAGTTATTTGGGTGTCT
TCGTTAGAGGAGTTTTTTAGTTTAGAACTTCCACCCTCCGTCTTTCTCATAGGTGGTGGGGACTTGTTTTCTTTGTTTTT
AGAAAATCAACTTCTGACAGGTTGTTTTGTAACTCACATTCATCGATGTTATGATGGTGATGCTTTCTTTCCCCTGTCTT
TATTGGTGGGGTGGAACAAGACTATTTTAGATGAACAAGAGGATTTGACGTTTTGTTATTATGAAAGTCTCGCCGATAGT
CACACGTAG

Upstream 100 bases:

>100_bases
CTATCGGTTTTGTTGCAACAGCAAGGGGTGAATTTCTTGCGAGTTCATGATGTTGAAGCCAACCAAAGGGTGTTATCTGC
CGCAGCATGGCCTGGAGTTT

Downstream 100 bases:

>100_bases
AGTTCATGTTTGTGATAATCTTTATGAAATTTTAGAAGAAGCTCTTCCTCCTTTAGCCGAGAATTCTATCATTGCTTTAT
CTTCTAAGGTTTTAAGTCTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MCKILGIVACSPSGVIGNQGKLPWSYSKDIEFFAKTIENKPLVMGRKTWEGLPEKYTKGRQIFVFSRSFYDNAEGVIWVS
SLEEFFSLELPPSVFLIGGGDLFSLFLENQLLTGCFVTHIHRCYDGDAFFPLSLLVGWNKTILDEQEDLTFCYYESLADS
HT

Sequences:

>Translated_162_residues
MCKILGIVACSPSGVIGNQGKLPWSYSKDIEFFAKTIENKPLVMGRKTWEGLPEKYTKGRQIFVFSRSFYDNAEGVIWVS
SLEEFFSLELPPSVFLIGGGDLFSLFLENQLLTGCFVTHIHRCYDGDAFFPLSLLVGWNKTILDEQEDLTFCYYESLADS
HT
>Mature_162_residues
MCKILGIVACSPSGVIGNQGKLPWSYSKDIEFFAKTIENKPLVMGRKTWEGLPEKYTKGRQIFVFSRSFYDNAEGVIWVS
SLEEFFSLELPPSVFLIGGGDLFSLFLENQLLTGCFVTHIHRCYDGDAFFPLSLLVGWNKTILDEQEDLTFCYYESLADS
HT

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=153, Percent_Identity=28.7581699346405, Blast_Score=73, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17505899, Length=147, Percent_Identity=31.2925170068027, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18359; Mature: 18359

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKILGIVACSPSGVIGNQGKLPWSYSKDIEFFAKTIENKPLVMGRKTWEGLPEKYTKGR
CCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECHHHCCCCHHHCCCC
QIFVFSRSFYDNAEGVIWVSSLEEFFSLELPPSVFLIGGGDLFSLFLENQLLTGCFVTHI
EEEEEECCCCCCCCCEEEHCCHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
HRCYDGDAFFPLSLLVGWNKTILDEQEDLTFCYYESLADSHT
HHHCCCCCEEEEHEEECCCHHHCCCCCCCEEEEEHHHCCCCC
>Mature Secondary Structure
MCKILGIVACSPSGVIGNQGKLPWSYSKDIEFFAKTIENKPLVMGRKTWEGLPEKYTKGR
CCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECHHHCCCCHHHCCCC
QIFVFSRSFYDNAEGVIWVSSLEEFFSLELPPSVFLIGGGDLFSLFLENQLLTGCFVTHI
EEEEEECCCCCCCCCEEEHCCHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
HRCYDGDAFFPLSLLVGWNKTILDEQEDLTFCYYESLADSHT
HHHCCCCCEEEEHEEECCCHHHCCCCCCCEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]