Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 89885978

GI number: 89885978

Start: 254487

End: 255713

Strand: Reverse

Name: pilT [H]

Synonym: Rfer_4493

Alternate gene names: 89885978

Gene position: 255713-254487 (Counterclockwise)

Preceding gene: 89885979

Following gene: 89885977

Centisome position: 99.33

GC content: 55.83

Gene sequence:

>1227_bases
ATGCCACATGACAATCAAATGGAACCGAGAGAGACGATGGAGGGGATGATCTCCATCATGGAGAAGATCGAATGGGCAAT
CTCGCTCCTGGTGAACCAATCGGTCAGTAACTTGATCCTTCGAGAAAACCGGTTCATTGAATGCGAAACTACCATGAACG
GTGAAGTCAGCATTGCCTCGGTTGACGACGCCTACTGCGAATCGCAGCTGCTGACGCGGGCTGAAATGAAAGCACTATTC
AATTTAGTCGATCAGAATTGGGAGGGGCGAATCCGCGCGGGCGGAATAGATGGGCCTATTCGCATGGGACCTTCAGCAAG
GTATCGGGCAAACATCTTCCTTTGGGGTGGTGAACAGAGTGACGTAGCTGATGGCCTTCCCGGGCTGTTGGGCTGCGTGA
TTCGGGTGATTCCCGAAGAAGTGCCGGACCTAACCACGCTGGGCCTGCCCGGACCCATGACCGTCCTGGCAGACGCGAAT
TATGGGTTGCTGCTTGTGGTCGGCCCCACGGGGTCTGGCAAGACAACCACCATGACAAGCTACTTTGATCACTTGAATAA
TCACCGAGTTGGTCACTTGGTCACGGTGGAAGATCCCATTGAATACGTGCTGAAAGAAAAGAAGTGCCGCATCACGCCTC
GCGAAGTTGGCACCAATGTGATATCGATCGCTGTGGGCGTACGAGATGCCATGCGCGAGATTCCCTTGGCGATCATGGTA
GGGGAAGTGCGCGACACAGAGACACTGGTCGAGACGTTTCGCGCCGCCCGAAGTGGCCACTATGTTGTCGCCACCAAACA
TGCACCAAATTTAGTGGCCGCTATCCGTTCTTTGGTTGACGATCTGCCCGGCGAGCCACGAGCCAATGCCGCCATGATTG
CCGAGACGTTGCTGGGTGTCATTTTTCAGGTCCGCGTCCCTAGCACTGAGTTTGGCAAATGGGAGTTCGTTCACGAAACA
ATGAATGTCACGGGCAATCAACGGGTGCAAGAGATGATCCTCAACCAGCAGTGGGTCGAATTACGGGGATATCTTGAGAA
CCCGGTGACGCAACCTGGCCAGCCTGGGCAGCGCAACGATGCTGTTGAGTCCCTGAATCGCAACCTGGCGAAAAAGGTGG
CTGAGGGCAAGATTACCGCGGCCGCAGCGGATCGCAGAGCTTACGACCGGCCTGGCCTGCGTCGGGCCATCGCAGCCGTG
CGCACTGAACTGACGCGTGAGGCGTGA

Upstream 100 bases:

>100_bases
CTGCAACTCGGCGCCGGCTGGAACCGCAGCAGTGAACGCAGAAGTTGGGCGCTCGTAAATTTCTGCCCTGAACAGGTTTT
CTTCGTTGAAGGTGCCACCT

Downstream 100 bases:

>100_bases
TCTAAGAGAACGAAAGCCACAATATGATTTTTGTACGTCGCCGCATGCAGGGTTTTACGCTGCCAGAACTTATGATCGGG
TTGGCCGTGTTTTCAATGCT

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPHDNQMEPRETMEGMISIMEKIEWAISLLVNQSVSNLILRENRFIECETTMNGEVSIASVDDAYCESQLLTRAEMKALF
NLVDQNWEGRIRAGGIDGPIRMGPSARYRANIFLWGGEQSDVADGLPGLLGCVIRVIPEEVPDLTTLGLPGPMTVLADAN
YGLLLVVGPTGSGKTTTMTSYFDHLNNHRVGHLVTVEDPIEYVLKEKKCRITPREVGTNVISIAVGVRDAMREIPLAIMV
GEVRDTETLVETFRAARSGHYVVATKHAPNLVAAIRSLVDDLPGEPRANAAMIAETLLGVIFQVRVPSTEFGKWEFVHET
MNVTGNQRVQEMILNQQWVELRGYLENPVTQPGQPGQRNDAVESLNRNLAKKVAEGKITAAAADRRAYDRPGLRRAIAAV
RTELTREA

Sequences:

>Translated_408_residues
MPHDNQMEPRETMEGMISIMEKIEWAISLLVNQSVSNLILRENRFIECETTMNGEVSIASVDDAYCESQLLTRAEMKALF
NLVDQNWEGRIRAGGIDGPIRMGPSARYRANIFLWGGEQSDVADGLPGLLGCVIRVIPEEVPDLTTLGLPGPMTVLADAN
YGLLLVVGPTGSGKTTTMTSYFDHLNNHRVGHLVTVEDPIEYVLKEKKCRITPREVGTNVISIAVGVRDAMREIPLAIMV
GEVRDTETLVETFRAARSGHYVVATKHAPNLVAAIRSLVDDLPGEPRANAAMIAETLLGVIFQVRVPSTEFGKWEFVHET
MNVTGNQRVQEMILNQQWVELRGYLENPVTQPGQPGQRNDAVESLNRNLAKKVAEGKITAAAADRRAYDRPGLRRAIAAV
RTELTREA
>Mature_407_residues
PHDNQMEPRETMEGMISIMEKIEWAISLLVNQSVSNLILRENRFIECETTMNGEVSIASVDDAYCESQLLTRAEMKALFN
LVDQNWEGRIRAGGIDGPIRMGPSARYRANIFLWGGEQSDVADGLPGLLGCVIRVIPEEVPDLTTLGLPGPMTVLADANY
GLLLVVGPTGSGKTTTMTSYFDHLNNHRVGHLVTVEDPIEYVLKEKKCRITPREVGTNVISIAVGVRDAMREIPLAIMVG
EVRDTETLVETFRAARSGHYVVATKHAPNLVAAIRSLVDDLPGEPRANAAMIAETLLGVIFQVRVPSTEFGKWEFVHETM
NVTGNQRVQEMILNQQWVELRGYLENPVTQPGQPGQRNDAVESLNRNLAKKVAEGKITAAAADRRAYDRPGLRRAIAAVR
TELTREA

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=194, Percent_Identity=34.5360824742268, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1789723, Length=144, Percent_Identity=37.5, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786296, Length=132, Percent_Identity=38.6363636363636, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 45050; Mature: 44919

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHDNQMEPRETMEGMISIMEKIEWAISLLVNQSVSNLILRENRFIECETTMNGEVSIAS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEE
VDDAYCESQLLTRAEMKALFNLVDQNWEGRIRAGGIDGPIRMGPSARYRANIFLWGGEQS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCC
DVADGLPGLLGCVIRVIPEEVPDLTTLGLPGPMTVLADANYGLLLVVGPTGSGKTTTMTS
CHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHH
YFDHLNNHRVGHLVTVEDPIEYVLKEKKCRITPREVGTNVISIAVGVRDAMREIPLAIMV
HHHHHCCCCCEEEEEECHHHHHHHHHHCCCCCHHHHCCCEEEEHHHHHHHHHHCCEEEEE
GEVRDTETLVETFRAARSGHYVVATKHAPNLVAAIRSLVDDLPGEPRANAAMIAETLLGV
CCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
IFQVRVPSTEFGKWEFVHETMNVTGNQRVQEMILNQQWVELRGYLENPVTQPGQPGQRND
HHEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
AVESLNRNLAKKVAEGKITAAAADRRAYDRPGLRRAIAAVRTELTREA
HHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PHDNQMEPRETMEGMISIMEKIEWAISLLVNQSVSNLILRENRFIECETTMNGEVSIAS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEE
VDDAYCESQLLTRAEMKALFNLVDQNWEGRIRAGGIDGPIRMGPSARYRANIFLWGGEQS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCC
DVADGLPGLLGCVIRVIPEEVPDLTTLGLPGPMTVLADANYGLLLVVGPTGSGKTTTMTS
CHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHH
YFDHLNNHRVGHLVTVEDPIEYVLKEKKCRITPREVGTNVISIAVGVRDAMREIPLAIMV
HHHHHCCCCCEEEEEECHHHHHHHHHHCCCCCHHHHCCCEEEEHHHHHHHHHHCCEEEEE
GEVRDTETLVETFRAARSGHYVVATKHAPNLVAAIRSLVDDLPGEPRANAAMIAETLLGV
CCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
IFQVRVPSTEFGKWEFVHETMNVTGNQRVQEMILNQQWVELRGYLENPVTQPGQPGQRND
HHEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
AVESLNRNLAKKVAEGKITAAAADRRAYDRPGLRRAIAAVRTELTREA
HHHHHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]