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The map label for this gene is proP [H]

Identifier: 89257187

GI number: 89257187

Start: 1876925

End: 1878211

Strand: Direct

Name: proP [H]

Synonym: FTL_1948

Alternate gene names: 89257187

Gene position: 1876925-1878211 (Clockwise)

Preceding gene: 89257183

Following gene: 89257189

Centisome position: 98.99

GC content: 29.68

Gene sequence:

>1287_bases
GTGATTAGGTACTTTTATTCAAACTTTGAAATTAGAGATAAAAAAATGAAAGACTTACAATTAAAAACTATTTTTCTTTG
TTCTTTAGGTATGGTTCTAGAATATTTTGACTTTATAATATATCTGAGCTTTTCTGAAGTAATAGGAACTCATTTACTTC
ATAGTTCTAACTCCCAACTTATTGTACTTTTGATATTTGCATTAGGTTTTATTACAAGACCTATTGGCGGTATTATCTTT
GGTCAGTTTGGTGATAGAGTTGGACGCAAAAAAGTATTAATAGCTGCTGTACTTATGATGACAATTTGTACGTTAGGTAT
TGGTCTTATTCCTGATGCTTCCGTTATTGGGATATATGCTGGTTTACTATTACTTACTTTTCGTATTATGCAAGGTCTTT
CTGCTGGTGGAGAGGTCAATAGTGCATTTACATATTTAAATGAAACTATCATTACTAACAAAATATATCCTATCGCAGCA
ATAATGATCTCGGCAGCTATAGGTGTAATGCTTGCTAAACTACTAACAATAATACTTAATTTTATTTTCCCTGAGAATTA
TGCTTGGAGAATTGCTTTTATACTCGGATCTACGCTAGGGATTATTACTTTTTATATAAGAAAAGAGCTACATGAGAGTC
CTCTTTTTACACAATTATTACAAACTAATCAACTGACAAAAAATCCAATTGTTGAGCTATTTAAACATTACAAAATGCCG
TTATTAGGCTGTTTTTTAATGATTGCTAATGTTATGTCTTGTATTACATTGTTCTATTATGCTTTTCCGAGCTATTTAGC
AAATCTATCTAGTTATAGCTCTAACAGTATGCTCAAGATTAGTATTATAGGCTTACTTATGATGTGTATATTTGGCTTAC
TATCAGCATTACTAATAAAGAAAGATAGCATTCCAAAATTGGATAATTGGTTCTTACTTTTATCATCAATATTATCTATC
TGGTTATGCTATGCTTTTATTCATAACTCACACTATATGGTTGCGTATTTAGTCTATAGTATTTTATTTAGTGCTTATAT
CATGATTGCTCAATATAGATGTATTAATGCTCTGCCTGTCAAAGTAAGATTAGTTGGTATGGGAATTGGTTATGGTTTAG
GTTCTGCTATTTTCGTTGGATTTATGCCTCTTATAACACAGTCTCTTATTTTAGAGTATCATTCACTCTATATCCCTGCT
TTTATTGCTATTATTAAAGCAATATTATCTTTAGTAGGTTCTATTATTCTAAATATAACTTCCCATACTAAAAAAATAAA
TTTATGA

Upstream 100 bases:

>100_bases
AGCAAAAATGATTGTTTTTTTATTTTTTAAAAAACCATATTCACTATAGCTTCATTATTTTCATGATATTATCTTACAAA
TTATCTAACCTAATTATTAT

Downstream 100 bases:

>100_bases
AAAAAAGTATCAGCAGCAAAAGTACAGTAATGAATGTTATGCAAAAGCTTTTTAATGTAAATGTAGGTCTTTTACTTGAA
ATATAACTAGGACAGTGTAC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MIRYFYSNFEIRDKKMKDLQLKTIFLCSLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQLIVLLIFALGFITRPIGGIIF
GQFGDRVGRKKVLIAAVLMMTICTLGIGLIPDASVIGIYAGLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAA
IMISAAIGVMLAKLLTIILNFIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMP
LLGCFLMIANVMSCITLFYYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIKKDSIPKLDNWFLLLSSILSI
WLCYAFIHNSHYMVAYLVYSILFSAYIMIAQYRCINALPVKVRLVGMGIGYGLGSAIFVGFMPLITQSLILEYHSLYIPA
FIAIIKAILSLVGSIILNITSHTKKINL

Sequences:

>Translated_428_residues
MIRYFYSNFEIRDKKMKDLQLKTIFLCSLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQLIVLLIFALGFITRPIGGIIF
GQFGDRVGRKKVLIAAVLMMTICTLGIGLIPDASVIGIYAGLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAA
IMISAAIGVMLAKLLTIILNFIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMP
LLGCFLMIANVMSCITLFYYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIKKDSIPKLDNWFLLLSSILSI
WLCYAFIHNSHYMVAYLVYSILFSAYIMIAQYRCINALPVKVRLVGMGIGYGLGSAIFVGFMPLITQSLILEYHSLYIPA
FIAIIKAILSLVGSIILNITSHTKKINL
>Mature_428_residues
MIRYFYSNFEIRDKKMKDLQLKTIFLCSLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQLIVLLIFALGFITRPIGGIIF
GQFGDRVGRKKVLIAAVLMMTICTLGIGLIPDASVIGIYAGLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAA
IMISAAIGVMLAKLLTIILNFIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMP
LLGCFLMIANVMSCITLFYYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIKKDSIPKLDNWFLLLSSILSI
WLCYAFIHNSHYMVAYLVYSILFSAYIMIAQYRCINALPVKVRLVGMGIGYGLGSAIFVGFMPLITQSLILEYHSLYIPA
FIAIIKAILSLVGSIILNITSHTKKINL

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=449, Percent_Identity=28.9532293986637, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789941, Length=297, Percent_Identity=32.996632996633, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1788942, Length=411, Percent_Identity=28.2238442822384, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788292, Length=395, Percent_Identity=27.3417721518987, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082404, Length=406, Percent_Identity=25.1231527093596, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082231, Length=283, Percent_Identity=21.9081272084806, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47980; Mature: 47980

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYFYSNFEIRDKKMKDLQLKTIFLCSLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQL
CCEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IVLLIFALGFITRPIGGIIFGQFGDRVGRKKVLIAAVLMMTICTLGIGLIPDASVIGIYA
HHHHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAAIMISAAIGVMLAKLLTIILN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMP
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCH
LLGCFLMIANVMSCITLFYYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC
KDSIPKLDNWFLLLSSILSIWLCYAFIHNSHYMVAYLVYSILFSAYIMIAQYRCINALPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
KVRLVGMGIGYGLGSAIFVGFMPLITQSLILEYHSLYIPAFIAIIKAILSLVGSIILNIT
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHTKKINL
HCCCCCCC
>Mature Secondary Structure
MIRYFYSNFEIRDKKMKDLQLKTIFLCSLGMVLEYFDFIIYLSFSEVIGTHLLHSSNSQL
CCEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
IVLLIFALGFITRPIGGIIFGQFGDRVGRKKVLIAAVLMMTICTLGIGLIPDASVIGIYA
HHHHHHHHHHHHHCHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GLLLLTFRIMQGLSAGGEVNSAFTYLNETIITNKIYPIAAIMISAAIGVMLAKLLTIILN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFPENYAWRIAFILGSTLGIITFYIRKELHESPLFTQLLQTNQLTKNPIVELFKHYKMP
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCH
LLGCFLMIANVMSCITLFYYAFPSYLANLSSYSSNSMLKISIIGLLMMCIFGLLSALLIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC
KDSIPKLDNWFLLLSSILSIWLCYAFIHNSHYMVAYLVYSILFSAYIMIAQYRCINALPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
KVRLVGMGIGYGLGSAIFVGFMPLITQSLILEYHSLYIPAFIAIIKAILSLVGSIILNIT
EEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SHTKKINL
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]