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The map label for this gene is purH [H]

Identifier: 89257172

GI number: 89257172

Start: 1860055

End: 1861602

Strand: Reverse

Name: purH [H]

Synonym: FTL_1929

Alternate gene names: 89257172

Gene position: 1861602-1860055 (Counterclockwise)

Preceding gene: 89257175

Following gene: 89257171

Centisome position: 98.19

GC content: 36.5

Gene sequence:

>1548_bases
ATGTCAAAAATAAAAAGGGTACTAATATCTGTATCTGATAAAACAGGTATCATTGAATTTGCCAAAGAATTAAGTAAGTA
TAACATCGAAATACTTTCAACAGGTGGAACTTCAAAAACCCTTAAAGATGCTGGAATAAACGTAATAGATGTTTCAGAGC
ATACACAATTTCCAGAAATTATGAATGGTCGTGTAAAAACACTTCATCCACTAATTCATGGTGGTATCTTAGCCGATAGA
GATAATCCTGAGCATCTCGATGCGATGCTAAAAAATCATATTGGACAAATAGATATGGTAGTCGTTAACTTATACCCTTT
TGTCAATACAGTTAAATCCGGTGCTGATTTTGATACCTGTATCGAAAACATAGATATCGGCGGACCTTCTATGGTGAGAT
CATGTGCAAAAAATCATAAACACACAACTATAGTGACCAACCCTAGTCAATATGATCTAGTTATCAAAGAGATGGCTGAG
AATGATGGCAAAACTACTCTAGCAACACGTCGTGAATTTGCTAAAGAAGCGTTTGCACATACTGCCGAATATGATAGTCA
TATTGCAAACTGGTTTAGTAAAGAGCTAGATGAAGAGTATCCTGAGAAACTTTTTTCTGTAGGAACTCTAAAACAGGTTT
TAAGATATGGCGAAAATCCTCATCAAAAAGCAGCTGTATACTCTACAGATGCTGACTTTGTAAGCATTACAAATGCAAGA
CAATTACAAGGCAAAGAATTATCGTATAATAACCTAAATGATGCCGATGGTGCTTTTGAGATGGTTTGTGAGTATTCAGA
ACCCTCTTGTGCAATTATCAAACATGCTAATCCTTGTGGTATCGCTTCTGGTAAAAATGCTTTTGAAGCTTGGACAAAAG
CTCTAGCTTGTGATCCAATTTCAGCTTTTGGTGGAATTGTTGCGTTTAACTGTGAAGTTGACAAAGAGTTTGCTGAATCT
CTAGGTAAAATGTTTTTAGAAGTAATAATTGCACCAAGCTATACTCAAGAAGCTCTAGATATTTTAGCAGCTAAGAAAAA
CTTACGCGTATTAGAAACTGGTAAAGTTTTTGATACAAAATCATCACGTATGATAAGTAAAAATATTCATGGTGGAATAC
TATCTCAATCTTATGATAACGGTGGCGTTGACATTAATTACTGTAAAGTTGTTACAGATAGAGCGCCAACTGACAAAGAG
TGGGCTGATTTAATGTTTGCCTGGAAAGCTGTTAAATACGTCAAATCAAATGCAATTGTCTATGCTAGAGATACCCAAAC
AGTAGGAATTGGTGCTGGACAGATGAGTCGAGTTGATTCTGCACGTATTGGTGCTGAAAAAGCCCAAGCTCATCAAGGTG
CTAAAGGTTCTGCTGTCGCATCTGATGCTTTCTTCCCTTTTGCTGACGGTTTAGAAGAATGTATCAAAGCTGGAGCAACA
GCGGTAATTCAACCAGGCGGTTCAAAAAATGATCAAGAAGTTATTGATGCTGCTAACAAAGCTGGTATCACTATGGTCTT
CACAAATATGAGACATTTTAGACACTAA

Upstream 100 bases:

>100_bases
ATATCGTTTAACAAAAATGTTTTAACTAAGAATTTATGATTAAAATTGTTATAATGATAGTTAAAATCTGACTTTAAATA
AATAATAACAAGGAACAAGC

Downstream 100 bases:

>100_bases
ATTAATAAATCTTTCTCCAAGTTGGATAATGTTATTTATTGTATTTTAATTGCTAGAACATTCTTTTAAGATAACGCCGA
AGTTAAAAGCGGCGATATTA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEIMNGRVKTLHPLIHGGILADR
DNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTCIENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAE
NDGKTTLATRREFAKEAFAHTAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADFVSITNAR
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPISAFGGIVAFNCEVDKEFAES
LGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTKSSRMISKNIHGGILSQSYDNGGVDINYCKVVTDRAPTDKE
WADLMFAWKAVKYVKSNAIVYARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH

Sequences:

>Translated_515_residues
MSKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEIMNGRVKTLHPLIHGGILADR
DNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTCIENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAE
NDGKTTLATRREFAKEAFAHTAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADFVSITNAR
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPISAFGGIVAFNCEVDKEFAES
LGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTKSSRMISKNIHGGILSQSYDNGGVDINYCKVVTDRAPTDKE
WADLMFAWKAVKYVKSNAIVYARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH
>Mature_514_residues
SKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEIMNGRVKTLHPLIHGGILADRD
NPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTCIENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAEN
DGKTTLATRREFAKEAFAHTAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADFVSITNARQ
LQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPISAFGGIVAFNCEVDKEFAESL
GKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTKSSRMISKNIHGGILSQSYDNGGVDINYCKVVTDRAPTDKEW
ADLMFAWKAVKYVKSNAIVYARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGATA
VIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=478, Percent_Identity=38.7029288702929, Blast_Score=288, Evalue=1e-77,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=52.5519848771267, Blast_Score=527, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=30.016583747927, Blast_Score=244, Evalue=7e-65,
Organism=Caenorhabditis elegans, GI71985574, Length=341, Percent_Identity=26.9794721407625, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=35.5179704016913, Blast_Score=259, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=35.3065539112051, Blast_Score=255, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24649832, Length=477, Percent_Identity=36.8972746331237, Blast_Score=265, Evalue=5e-71,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56312; Mature: 56181

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEI
CCHHHEEHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCEEECCCCCCCHHH
MNGRVKTLHPLIHGGILADRDNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTC
HCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCHHHH
IENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAENDGKTTLATRREFAKEAFAH
HHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADFVSITNAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCHH
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPI
HCCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCH
SAFGGIVAFNCEVDKEFAESLGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTK
HHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEECCC
SSRMISKNIHGGILSQSYDNGGVDINYCKVVTDRAPTDKEWADLMFAWKAVKYVKSNAIV
CCHHHHHCCCCCHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
YARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
EEECCCEEECCCCHHHHHHHHHCCHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCE
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH
EEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
SKIKRVLISVSDKTGIIEFAKELSKYNIEILSTGGTSKTLKDAGINVIDVSEHTQFPEI
CHHHEEHHCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCEEECCCCCCCHHH
MNGRVKTLHPLIHGGILADRDNPEHLDAMLKNHIGQIDMVVVNLYPFVNTVKSGADFDTC
HCCCHHHHHHHHHCCEEECCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCHHHH
IENIDIGGPSMVRSCAKNHKHTTIVTNPSQYDLVIKEMAENDGKTTLATRREFAKEAFAH
HHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
TAEYDSHIANWFSKELDEEYPEKLFSVGTLKQVLRYGENPHQKAAVYSTDADFVSITNAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCHH
QLQGKELSYNNLNDADGAFEMVCEYSEPSCAIIKHANPCGIASGKNAFEAWTKALACDPI
HCCCCCCCCCCCCCCCCHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCH
SAFGGIVAFNCEVDKEFAESLGKMFLEVIIAPSYTQEALDILAAKKNLRVLETGKVFDTK
HHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCEECCC
SSRMISKNIHGGILSQSYDNGGVDINYCKVVTDRAPTDKEWADLMFAWKAVKYVKSNAIV
CCHHHHHCCCCCHHCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCEEE
YARDTQTVGIGAGQMSRVDSARIGAEKAQAHQGAKGSAVASDAFFPFADGLEECIKAGAT
EEECCCEEECCCCHHHHHHHHHCCHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHCCCE
AVIQPGGSKNDQEVIDAANKAGITMVFTNMRHFRH
EEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA