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The map label for this gene is gadA [H]

Identifier: 89257118

GI number: 89257118

Start: 1793853

End: 1794974

Strand: Reverse

Name: gadA [H]

Synonym: FTL_1863

Alternate gene names: 89257118

Gene position: 1794974-1793853 (Counterclockwise)

Preceding gene: 89257119

Following gene: 89257117

Centisome position: 94.67

GC content: 37.88

Gene sequence:

>1122_bases
ATGGCATTACATGGTAAAAAAGATACAATTGATAACCTTGATTTTTTTGAACAATCTTTACCAAAGTTTAAACTTCCACT
AAATTCACAAGATCCTTTAGAAGTGTATCAAGAAATCAAAGATGAGTTAATGCTAGATGGTAATTCGAAGCAAAATTTAG
AGACGTTTTGCCAAACGGAAGTTGATGATTTCATTCACAAGCTTATGGATGATTGCATTGATAAAAATATGATTGACAAA
GATGAATATCCGCAGACTGCGGAAATAGAGTCTCGCTGTGTAAATATCCTTGCAAACCTATGGAACTCTTCAGCAGAAAA
TGCAATTGGCTGCTCAACGACTGGCTCATCAGAGGCTGCTATGCTTGGCGGTATGGCAATGAAGTGGCGTTGGCGAGATA
AAATGAAAGCGCAAGGTAAAGATTATACAAAACCTAATCTTGTTACAGGACCTGTGCAAGTGTGCTGGCATAAATTTGCT
AGATATTGGGATATTGAGCTTAGAGAAATTCCAATGTCGAATGAAAGTTTAATAATGACTCCAGAGGCTGTGCTTGAAAG
ATGTGATGAAAATACAATCGGTGTGGTGCCAACTCTTGGAGTGACTTTTACAGGGCAGTATGAGCCAGTAGAACAGGTAT
GTAAAGCTCTTGATGATTTTGAACGACAAACAGGCGTAGATATTCCAGTTCATGTTGATGCAGCATCTGGAGGTTTTTTG
GCACCATTTGTAGAGCCTGAACTTAAGTGGGACTTTAGATTACCAATATCGCCACTTGGGGTTGGTTGGGTTATTTGGGC
TGATAAAAAGTACTTACCAGATGATTTGATTTTTAATGTTAATTACCTAGGTGGTAATATGCCAACATTTGCACTGAATT
TCTCGCGACCTGGTGGTCAGATAGTAGCCCAGTATTATAATTTTGTTAGGTTGGGTTTTGAAGGCTATAAGAAAGTTCAT
CAGCTTTGCTATGATATTGCAGAATATATTGCTAAAGAGCTTAGAAAAATGGAAATTTTTGAAATTATTCATGCTGGAGA
GGGTGGTATTCCTGCGGTTAGCTGGTCACTTAAAGCAACTAAAGAGTATAGTTTGTTTGATATATCTGAGAGTTCGCGCT
AA

Upstream 100 bases:

>100_bases
ATAGTTTCGATATGTTGTAAAAATATTGTCGCTCAGAAAATTAATCTATTCGAGATAGTAGATTTTAAATTTAAACAGAT
ACAGCTTTAGAGAGAGGATT

Downstream 100 bases:

>100_bases
AGGTTGGCAAATAGCAGCTTACACAATGCCAAACAATCGTGAAGATTTAGTGGTTATGCGTGTATTAGTTAGAAGAGGCT
TTAGTTATGACTTGGCACAG

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLETFCQTEVDDFIHKLMDDCIDKNMIDK
DEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAAMLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFA
RYWDIELREIPMSNESLIMTPEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHVDAASGGFL
APFVEPELKWDFRLPISPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQIVAQYYNFVRLGFEGYKKVH
QLCYDIAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKATKEYSLFDISESSR

Sequences:

>Translated_373_residues
MALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLETFCQTEVDDFIHKLMDDCIDKNMIDK
DEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAAMLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFA
RYWDIELREIPMSNESLIMTPEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHVDAASGGFL
APFVEPELKWDFRLPISPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQIVAQYYNFVRLGFEGYKKVH
QLCYDIAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKATKEYSLFDISESSR
>Mature_372_residues
ALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLETFCQTEVDDFIHKLMDDCIDKNMIDKD
EYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAAMLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFAR
YWDIELREIPMSNESLIMTPEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHVDAASGGFLA
PFVEPELKWDFRLPISPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQIVAQYYNFVRLGFEGYKKVHQ
LCYDIAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKATKEYSLFDISESSR

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=331, Percent_Identity=23.2628398791541, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787769, Length=370, Percent_Identity=56.4864864864865, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1789934, Length=370, Percent_Identity=56.4864864864865, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17557272, Length=291, Percent_Identity=24.3986254295533, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25148342, Length=319, Percent_Identity=21.0031347962382, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323905, Length=344, Percent_Identity=43.6046511627907, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI21355963, Length=314, Percent_Identity=25.4777070063694, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654344, Length=314, Percent_Identity=25.4777070063694, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 42363; Mature: 42231

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLETFCQTE
CCCCCCCCCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHH
VDDFIHKLMDDCIDKNMIDKDEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
MLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFARYWDIELREIPMSNESLIMT
HHCCEEEEEEHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCCEEEC
PEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHVDAASGGFL
HHHHHHHCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
APFVEPELKWDFRLPISPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQ
CCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCHHEEEEEECCCCCCEEEEEECCCCHH
IVAQYYNFVRLGFEGYKKVHQLCYDIAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
KEYSLFDISESSR
CCCEEEECCCCCC
>Mature Secondary Structure 
ALHGKKDTIDNLDFFEQSLPKFKLPLNSQDPLEVYQEIKDELMLDGNSKQNLETFCQTE
CCCCCCCCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHH
VDDFIHKLMDDCIDKNMIDKDEYPQTAEIESRCVNILANLWNSSAENAIGCSTTGSSEAA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
MLGGMAMKWRWRDKMKAQGKDYTKPNLVTGPVQVCWHKFARYWDIELREIPMSNESLIMT
HHCCEEEEEEHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHCCCEEEECCCCCCCEEEC
PEAVLERCDENTIGVVPTLGVTFTGQYEPVEQVCKALDDFERQTGVDIPVHVDAASGGFL
HHHHHHHCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEE
APFVEPELKWDFRLPISPLGVGWVIWADKKYLPDDLIFNVNYLGGNMPTFALNFSRPGGQ
CCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCHHEEEEEECCCCCCEEEEEECCCCHH
IVAQYYNFVRLGFEGYKKVHQLCYDIAEYIAKELRKMEIFEIIHAGEGGIPAVSWSLKAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
KEYSLFDISESSR
CCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]