Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 89257073

GI number: 89257073

Start: 1744172

End: 1745641

Strand: Reverse

Name: nusA [H]

Synonym: FTL_1810

Alternate gene names: 89257073

Gene position: 1745641-1744172 (Counterclockwise)

Preceding gene: 89257074

Following gene: 89257072

Centisome position: 92.07

GC content: 34.08

Gene sequence:

>1470_bases
ATGAGCAAAGAATTATTGTTAGTATTAGAAACTGTAGCAAATGAAAAAGATATCTCAAAAGATCTTTTATTTGAAGCTAT
GGAAGAGGCTTTGGCTATTATAACTAAGAAAGAACTTGATGAGCATATGAATATCGAGGTTAAAATCGATAGAGTTACGG
GTGATTTCAAGGCTGATAGAGTTTGGCATATTGTTTCTGAAGATGAAGATTTAATTGATTATTCAAAAGAGCTTTATGAA
GATGTCGTTCAAGAAAAAGGCTATAATGTAAAAGCTGGCGATGTTATGCGTGAACCTGTTGAGATCAAAGAATATGGGCG
TATAGCTGCGACAATGGCTAAGCAAATTCTAATGAAGAAAATCAAAAATTTTGAAAGAGAAAAGTCAGCTAGATTTTATC
AAAATAAAATTGGCGATATTGTTTATGGCGAGATAAAGCGCGCTACATATGAAATCTTAGTTGTTGATTTGGGCAATAAT
GCTGAGGGGATTTTACCTAAAAAAGATCTAATAGCTAGAGAAAGATATCGAGTTGGTGACAAAATAAGAGCATGTGTTGA
AGCTGTTGAGAGTGATGAGTCTGGCAAACCAAATACGATTATGTTAAGTCGTTCAAGCAATACTATGCTTAAAGCTTTAT
TTAAGCTTGAGGTTCCGGAGGTTGAAGAAGAGCTTATTAATATAGTGAATGTAGTTCGTGAACATGGTTTTAGGTCTAAA
GTAACTGTTAAAAGTAATGATCAAAGAATAGATCCTTGTGGTGCCTGTGTTGGTGTGAGAGGTTCTAGAATACATTCTAT
AATGAGTGAGCTAAATGGTGAGAAGGTTGATGTTATACTTTGGAATGAAGATATGGTTCAGTATGCAATTAACTCATTAT
CACCAGTTGATGCAGCAGACATCTTAGAAGTGAATGTTGATGAAGAAACTAATTCGATGGATATTGTTGTTAAGCAAGAA
AGTTTATCTAAAGCAATTGGTAAAAATGGTGTTAATGTAAGATTAGCAAGTTCGTTAATAGGTTGGAAAATTAATGTTCT
ATCAGATGCTGAGCAGGAAGAAAAACAAATGTCTATAGTAGAGAAATTTGTTGAAGCCTTAGATATAGATCACAATTTTG
CATTAGTATTGATTGAAGAGGGTATAGAGACTCTTGAAGATTTAGCATATTTAGATAGAGCTGAGCTTTTAGAAATTGAA
GGGTTTGATGAAGAGATCGTTGATGAGCTTCAAGAGAGAGCAAAAGCAGTTATTTTATCTCAAGCTTTAGGAGGTAAAAA
ACCAGCTCAAGATTTGTTAGATATGCAAGGTATGAGTCTGGATTTGGCTGAGCAGCTAGCGCAAAATAATATAGTTACTA
TGGAAGATCTAGCAGAGCTATCTGTAGATGAGTTGCTTGATATTGTGGATATAGATGAAGAGCAAGCAACTAATTTAATT
ATGCAAGCAAGAGCTCCTTGGTTTGAATAA

Upstream 100 bases:

>100_bases
TTCTAAACTTGGAAGATGGTAAAGAAATAAGTTTTGATTTTGACGAGCTTAAAAAACTAAGGGTATCACCTGATTTTAGT
TAGATAGAGGAATTTAAAAT

Downstream 100 bases:

>100_bases
TAAAAGTACATATTAGGAATAGTACAAAGGAGAGGGAAATGGCAGAGATTACAGTTGGGCAGTTAGCACAGCAAACAAAT
AAAGAAGTAGATGCACTACT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADRVWHIVSEDEDLIDYSKELYE
DVVQEKGYNVKAGDVMREPVEIKEYGRIAATMAKQILMKKIKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNN
AEGILPKKDLIARERYRVGDKIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREHGFRSK
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAADILEVNVDEETNSMDIVVKQE
SLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIVEKFVEALDIDHNFALVLIEEGIETLEDLAYLDRAELLEIE
GFDEEIVDELQERAKAVILSQALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
MQARAPWFE

Sequences:

>Translated_489_residues
MSKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADRVWHIVSEDEDLIDYSKELYE
DVVQEKGYNVKAGDVMREPVEIKEYGRIAATMAKQILMKKIKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNN
AEGILPKKDLIARERYRVGDKIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREHGFRSK
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAADILEVNVDEETNSMDIVVKQE
SLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIVEKFVEALDIDHNFALVLIEEGIETLEDLAYLDRAELLEIE
GFDEEIVDELQERAKAVILSQALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
MQARAPWFE
>Mature_488_residues
SKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADRVWHIVSEDEDLIDYSKELYED
VVQEKGYNVKAGDVMREPVEIKEYGRIAATMAKQILMKKIKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNNA
EGILPKKDLIARERYRVGDKIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREHGFRSKV
TVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAADILEVNVDEETNSMDIVVKQES
LSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIVEKFVEALDIDHNFALVLIEEGIETLEDLAYLDRAELLEIEG
FDEEIVDELQERAKAVILSQALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLIM
QARAPWFE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=499, Percent_Identity=40.6813627254509, Blast_Score=393, Evalue=1e-110,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55180; Mature: 55048

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADR
CCCHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCC
VWHIVSEDEDLIDYSKELYEDVVQEKGYNVKAGDVMREPVEIKEYGRIAATMAKQILMKK
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNNAEGILPKKDLIARERYRVGD
HHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
KIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREHGFRSK
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAAD
EEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCCCC
ILEVNVDEETNSMDIVVKQESLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIV
EEEEECCCCCCCEEEEEEHHHHHHHHCCCCCEEEEECHHHCEEEEECCCCHHHHHHHHHH
EKFVEALDIDHNFALVLIEEGIETLEDLAYLDRAELLEIEGFDEEIVDELQERAKAVILS
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHHH
QALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
HHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHCCHHHHHHHHCCCHHHHHHHH
MQARAPWFE
HHCCCCCCC
>Mature Secondary Structure 
SKELLLVLETVANEKDISKDLLFEAMEEALAIITKKELDEHMNIEVKIDRVTGDFKADR
CCHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCC
VWHIVSEDEDLIDYSKELYEDVVQEKGYNVKAGDVMREPVEIKEYGRIAATMAKQILMKK
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHH
IKNFEREKSARFYQNKIGDIVYGEIKRATYEILVVDLGNNAEGILPKKDLIARERYRVGD
HHHHHHHHHHHHHHHHCCCHHHHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
KIRACVEAVESDESGKPNTIMLSRSSNTMLKALFKLEVPEVEEELINIVNVVREHGFRSK
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCE
VTVKSNDQRIDPCGACVGVRGSRIHSIMSELNGEKVDVILWNEDMVQYAINSLSPVDAAD
EEECCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCCCCC
ILEVNVDEETNSMDIVVKQESLSKAIGKNGVNVRLASSLIGWKINVLSDAEQEEKQMSIV
EEEEECCCCCCCEEEEEEHHHHHHHHCCCCCEEEEECHHHCEEEEECCCCHHHHHHHHHH
EKFVEALDIDHNFALVLIEEGIETLEDLAYLDRAELLEIEGFDEEIVDELQERAKAVILS
HHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHHHHHH
QALGGKKPAQDLLDMQGMSLDLAEQLAQNNIVTMEDLAELSVDELLDIVDIDEEQATNLI
HHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHCCHHHHHHHHCCCHHHHHHHH
MQARAPWFE
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]