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The map label for this gene is pilT [H]

Identifier: 89257035

GI number: 89257035

Start: 1702224

End: 1702832

Strand: Reverse

Name: pilT [H]

Synonym: FTL_1770

Alternate gene names: 89257035

Gene position: 1702832-1702224 (Counterclockwise)

Preceding gene: 89257036

Following gene: 89257032

Centisome position: 89.81

GC content: 37.44

Gene sequence:

>609_bases
TTGGCAGCATTAGTCAATGAAATAAATCATAAGGAAGATTCTCATATTCTAACTATTGAGGATCCCGTAGAATTTGTTCA
TACAAGTCAAAGATCACTAGTAAACCAACGTGAAGTTAAAAGAGATACAAAAAGCTTTAATGCGGCTCTTAAATCAGCAC
TAAGGGAGGATCCTGATTGTATTCTAGTTGGAGAGATGCGTGATTTAGAAACTATTAAATTAGCACTAGAGGCAGCTGAA
ACTGGGCATTTGGTTTTAGGGACATTACATACAATGTCAGCTATAAAAACTGTTGATAGAGTAATATCTGTATTTCCACC
TGCTGAGCAGGAGCTTGTACGCAACATGCTTGCGGAATCATTACAAATCGTTATATCTCAACGTTTACTTAAACGTAAGG
GCGGCGGGCGTGTCGCAGCATATGAAGTTCTTATTTCAAATACTGGTATTAGAAATATGATTAAAGAAAATAAACTTTCG
CAAATTTACACCGCACTACAAACTGGAACAGCTAAGGGAATGGCGACAATCGAACAGAGCATTGAGAATCTTCTTAAGGC
TGGAACAATTACACCAGAAGAAGCCGCTAAATACATTGTAGTAAGGTAA

Upstream 100 bases:

>100_bases
AGATCAATTTGGAGCACCAAGAATTCTTAAAGAAGTTTAAGCTAAAAAAGGTGGCTTAATTCTAGTAACAGGACCCACAG
GTTCAGGTAAGAGTAGTACA

Downstream 100 bases:

>100_bases
TCATTTAAAAATCTAATATTTATATTGCTAGCAACAAATATTCAGTTCATATTTTATAGTTAGTCAAAATTTTAGTTCTT
CATGAAAGGTTTTTTGTAAT

Product: Type IV pili nucleotide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIKLALEAAE
TGHLVLGTLHTMSAIKTVDRVISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLS
QIYTALQTGTAKGMATIEQSIENLLKAGTITPEEAAKYIVVR

Sequences:

>Translated_202_residues
MAALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIKLALEAAE
TGHLVLGTLHTMSAIKTVDRVISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLS
QIYTALQTGTAKGMATIEQSIENLLKAGTITPEEAAKYIVVR
>Mature_201_residues
AALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDCILVGEMRDLETIKLALEAAET
GHLVLGTLHTMSAIKTVDRVISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQ
IYTALQTGTAKGMATIEQSIENLLKAGTITPEEAAKYIVVR

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=190, Percent_Identity=49.4736842105263, Blast_Score=191, Evalue=2e-50,
Organism=Escherichia coli, GI1786296, Length=121, Percent_Identity=40.495867768595, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789723, Length=119, Percent_Identity=42.0168067226891, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 22344; Mature: 22213

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDC
CCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ILVGEMRDLETIKLALEAAETGHLVLGTLHTMSAIKTVDRVISVFPPAEQELVRNMLAES
EEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATIEQS
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IENLLKAGTITPEEAAKYIVVR
HHHHHHHCCCCHHHHCCEEECC
>Mature Secondary Structure 
AALVNEINHKEDSHILTIEDPVEFVHTSQRSLVNQREVKRDTKSFNAALKSALREDPDC
CHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ILVGEMRDLETIKLALEAAETGHLVLGTLHTMSAIKTVDRVISVFPPAEQELVRNMLAES
EEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LQIVISQRLLKRKGGGRVAAYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATIEQS
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IENLLKAGTITPEEAAKYIVVR
HHHHHHHCCCCHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]