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The map label for this gene is ampD [H]

Identifier: 89256996

GI number: 89256996

Start: 1659427

End: 1659951

Strand: Reverse

Name: ampD [H]

Synonym: FTL_1727

Alternate gene names: 89256996

Gene position: 1659951-1659427 (Counterclockwise)

Preceding gene: 89256998

Following gene: 89256991

Centisome position: 87.55

GC content: 32.38

Gene sequence:

>525_bases
ATGTTTAATCAAGGTTGGTATAAAAAAGCTAAATGTATTAAGAGTCCGAACTTTAATCAACGAGCTGATAAAAATGATAT
TAACCTAGTTGTAATACATTGTATAAGTTTGCCTGAAGGAGGATATGCCAATTGTAATGTTGAGAAATTTTTTACCAATC
AATTAGATTGTAGTTTGCATCCAAGTTTTGCAAGCCTTAAAGGTGTAGAAGTTTCAGCTCATTTTTATATAAAACGCGAT
GGTGAAATTGTGCAGTTTGTTTCTGTTGATGATAGAGCATGGCATGCAGGAGTAAGTGAATTTCAAGGTAGGCAAGGTTG
TAATGATTTTTCTGTAGGAATTGAGTTGCAGGGTACTGATAAAACAGCTTATACTGAACAACAATATCTTAGTTTGAATA
GTTTATTAAAAGATCTTAGGAAAGTATACCCAACATTATTAAATATTACTGGTCATGAAGATATTGCACCACAGAGAAAA
ACTGATCCTGGTAAATGTTTTGAATGGAATAAGGTTATCTGGTAG

Upstream 100 bases:

>100_bases
TGCAAGTTTATGGTCAAAGTAATTCATATTTAAATTTGTTTTAAAACAGCTAATTAGTTGGTAAAGTATAATATATCATA
AGCAAATTTCAAAAACTAAA

Downstream 100 bases:

>100_bases
AAATTCTAATTAATTGATAAAAAAATAAAAGTAAGTAAAAGAGTCTATTATTCAACACTGGCTCTATCACCATAGTTCTC
GAGCCAATCACGGCGATCTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MFNQGWYKKAKCIKSPNFNQRADKNDINLVVIHCISLPEGGYANCNVEKFFTNQLDCSLHPSFASLKGVEVSAHFYIKRD
GEIVQFVSVDDRAWHAGVSEFQGRQGCNDFSVGIELQGTDKTAYTEQQYLSLNSLLKDLRKVYPTLLNITGHEDIAPQRK
TDPGKCFEWNKVIW

Sequences:

>Translated_174_residues
MFNQGWYKKAKCIKSPNFNQRADKNDINLVVIHCISLPEGGYANCNVEKFFTNQLDCSLHPSFASLKGVEVSAHFYIKRD
GEIVQFVSVDDRAWHAGVSEFQGRQGCNDFSVGIELQGTDKTAYTEQQYLSLNSLLKDLRKVYPTLLNITGHEDIAPQRK
TDPGKCFEWNKVIW
>Mature_174_residues
MFNQGWYKKAKCIKSPNFNQRADKNDINLVVIHCISLPEGGYANCNVEKFFTNQLDCSLHPSFASLKGVEVSAHFYIKRD
GEIVQFVSVDDRAWHAGVSEFQGRQGCNDFSVGIELQGTDKTAYTEQQYLSLNSLLKDLRKVYPTLLNITGHEDIAPQRK
TDPGKCFEWNKVIW

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=172, Percent_Identity=49.4186046511628, Blast_Score=184, Evalue=3e-48,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=36.9230769230769, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 19825; Mature: 19825

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNQGWYKKAKCIKSPNFNQRADKNDINLVVIHCISLPEGGYANCNVEKFFTNQLDCSLH
CCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHEEECCCCCCEEC
PSFASLKGVEVSAHFYIKRDGEIVQFVSVDDRAWHAGVSEFQGRQGCNDFSVGIELQGTD
CCCHHCCCEEEEEEEEEECCCCEEEEEECCCCHHHCCHHHHCCCCCCCCEEEEEEEECCC
KTAYTEQQYLSLNSLLKDLRKVYPTLLNITGHEDIAPQRKTDPGKCFEWNKVIW
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MFNQGWYKKAKCIKSPNFNQRADKNDINLVVIHCISLPEGGYANCNVEKFFTNQLDCSLH
CCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHEEECCCCCCEEC
PSFASLKGVEVSAHFYIKRDGEIVQFVSVDDRAWHAGVSEFQGRQGCNDFSVGIELQGTD
CCCHHCCCEEEEEEEEEECCCCEEEEEECCCCHHHCCHHHHCCCCCCCCEEEEEEEECCC
KTAYTEQQYLSLNSLLKDLRKVYPTLLNITGHEDIAPQRKTDPGKCFEWNKVIW
CCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]