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The map label for this gene is dmlR [H]

Identifier: 89256845

GI number: 89256845

Start: 1496245

End: 1497165

Strand: Direct

Name: dmlR [H]

Synonym: FTL_1568

Alternate gene names: 89256845

Gene position: 1496245-1497165 (Clockwise)

Preceding gene: 89256843

Following gene: 89256846

Centisome position: 78.92

GC content: 28.99

Gene sequence:

>921_bases
ATGAAAATGTTATATGAGCTAAAACTATTTGTAGCTGTAATTAACTACGGTAACTTATCTGCTGCTGGACGAGAATTTTA
TTTATCTCCAGCTAGTATTAGTAATAAAATTAATGCCCTCGAAGAGTATTATCAAACCAAGCTTTTAATAAGAACTACAA
GAAAGATTGAACCTACTAAAGCAGGAGAAAAGCTTTATACTACTGCTATTAAATTATTAGAACAACTAGAGAACCTTCAA
GAAGATATTCAAAATAATGAAAACTCCGCAGAAGGTGTTATTAAGATAACGATTCCTTTTGACTTAGGTAAGCAGATAAT
CCTACCAATTTTAGATGAATTCAAAGAAAAAAATCCCAACATACAATACGATATAAATCTCTCTGATGATGTAATGTCTT
ATAACCAGTTTCCTTTTGATATAGCAATTAGATATGGCAATTTACCAGATAGTAACTTTATTGCAAAAAAGCTTGTCGAT
AACTATAGAATGTTATGTGCCAGCCCTGAATATCTAAAAAAGATGGACATATCTAACACTGATGATATAAACATATTACA
AAATTGTGATTTCATAACATTAAAAATAAACTCTAACACAATCAAAAAATGGTATTTAATTGATCAAGAGAATAATAAAC
ATGAAGTTAACATAAAGCCATCTTACATAGTTAATAATGGTTATATAAGCCGACAAATGTGTCTTAATGGAAATGGAATA
AGTGAAAAATCATATTGGGATGTCAAGCAAGATCTAAAATCAGGTAAATTAGTTCAGGTTTTACCAAATTATAGAGTAAC
TCTAAACAAAAAGGATAAGCCTGATAATATGATAAGCCTTCTATATCCGTCCGATCAGTTTCAACCTTACAGAATAAGAA
TGTTAAGTAAATTTATAGTAGATAGTTTCTCAAAATTCTAA

Upstream 100 bases:

>100_bases
TTATAAATTTAAACTTTCATTGCTTGACACCAATTATACTTTTAACTTTTTCAATGATAATATACAAAAATATTAAATGA
CTTTTTATAAAAACTAAAAA

Downstream 100 bases:

>100_bases
ATTTAGATATAATCTTGACTAAAAAAGTTGATTTCTTATACGATGATAAAAAATATATTTTTTGACTTAGATGGTACGCT
TGTAAATACTGTAGGTGATC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRTTRKIEPTKAGEKLYTTAIKLLEQLENLQ
EDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPNIQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVD
NYRMLCASPEYLKKMDISNTDDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIVDSFSKF

Sequences:

>Translated_306_residues
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRTTRKIEPTKAGEKLYTTAIKLLEQLENLQ
EDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPNIQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVD
NYRMLCASPEYLKKMDISNTDDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIVDSFSKF
>Mature_306_residues
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRTTRKIEPTKAGEKLYTTAIKLLEQLENLQ
EDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPNIQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVD
NYRMLCASPEYLKKMDISNTDDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIVDSFSKF

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=306, Percent_Identity=29.0849673202614, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789440, Length=296, Percent_Identity=31.0810810810811, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=27.4576271186441, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693193, Length=315, Percent_Identity=27.3015873015873, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=268, Percent_Identity=25.7462686567164, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789639, Length=267, Percent_Identity=22.8464419475655, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=25, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787128, Length=301, Percent_Identity=21.9269102990033, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=169, Percent_Identity=24.8520710059172, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788297, Length=199, Percent_Identity=23.6180904522613, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789173, Length=263, Percent_Identity=20.532319391635, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35542; Mature: 35542

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRTTRKIEPTK
CCHHHHHEEEHHEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
AGEKLYTTAIKLLEQLENLQEDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
IQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVDNYRMLCASPEYLKKMDISNT
EEEEECCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCHHHHHHCCCCCC
DDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
CCCEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCEEEECCEEEEEEEECCCCC
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIV
CCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
DSFSKF
HHHHCC
>Mature Secondary Structure
MKMLYELKLFVAVINYGNLSAAGREFYLSPASISNKINALEEYYQTKLLIRTTRKIEPTK
CCHHHHHEEEHHEECCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
AGEKLYTTAIKLLEQLENLQEDIQNNENSAEGVIKITIPFDLGKQIILPILDEFKEKNPN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
IQYDINLSDDVMSYNQFPFDIAIRYGNLPDSNFIAKKLVDNYRMLCASPEYLKKMDISNT
EEEEECCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHEEECCHHHHHHCCCCCC
DDINILQNCDFITLKINSNTIKKWYLIDQENNKHEVNIKPSYIVNNGYISRQMCLNGNGI
CCCEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEECCCEEEECCEEEEEEEECCCCC
SEKSYWDVKQDLKSGKLVQVLPNYRVTLNKKDKPDNMISLLYPSDQFQPYRIRMLSKFIV
CCCHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
DSFSKF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]