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The map label for this gene is lpdA [H]

Identifier: 89255709

GI number: 89255709

Start: 262750

End: 264168

Strand: Direct

Name: lpdA [H]

Synonym: FTL_0277

Alternate gene names: 89255709

Gene position: 262750-264168 (Clockwise)

Preceding gene: 89255708

Following gene: 89255710

Centisome position: 13.86

GC content: 33.33

Gene sequence:

>1419_bases
ATGATTAAAGCAGATATTTGTATTATTGGTGGAGGGTCTGGTGGGCTTTCTGTTGCGGCTGGGGCAGTACAAATGGGTGC
AAAAGTCGTTTTGTGTGAAGGCAATAAAATGGGTGGTGATTGTCTTAATTATGGTTGTGTACCTTCTAAAGCAATTATAG
AAGCTTCTAGAGTTATTGCAAAAGTAAATAAAGCACAAGCTTTTGGTATAAACATAGATAACAATAACATTGAGATTGAT
TATAAAAAAGTACAAGAGCATATCAAAATCACAATAGCAAAAATAGAGCCACATGATTCAGTTGAACGTTTTGAAACTTT
AGGTGTTAATGTTATTCAAGAATATGCTCAAATAATTGATCAATATACAGTCAAAGCAGGAGATAATTTTATTAAAGCTA
GGTATATTGTCATAGCAACAGGATCAAGAGCAGCTATACCTAAAATTAAAGGCTTAGCTGAGGTAAACTACTTAACTAAT
GAAACCATTTTTGATCTTAAAGAAAAGCCTGAGCATTTGATGATCGTTGGTGGCGGACCTATTGGTGTTGAATTGGCGCA
GGCTTATGCTTTACTTGGTAGCAAAGTCACTATTTTTGAAGCATCAGATACTATCTTAGGTGTACTTGATAATGACTGCC
GAAAAATTATACTTAAAGAATTTGATCGTTTAGGTATTAGCATAATAACAAATGTAAATATTAGTGAGATAGCTCAAGAT
GACCAAGAGATAAATCTTTACTGTGGTAGTAAGCTTTATCAAGGTTCTCATTTGTTAATCGCCGCTGGAAGAGTGCCAAA
TTTAGATAAGCTTGATTTAGATAATGTTGGCATTAAATATACTTCAAGAGGTATAAAGGTTGATAGCCGTCTAAGGACAA
ATCACAAAAATATTTATGCAATTGGAGATGTTGTAGGAGGGTACCAGTTTACTCATGTTGCTGGTTATCACGCTGGTGTT
GTGATTCAGAATATTCTTTTTAAACTACCAATAAAAGTCGATTATAATAGTTTACCTTGGTCTTTATATACTAGCCCAGA
AGTAGCTCATGTTGGACAAAATATTGCACAGACGCAAACTCATGGTGCAAAAATTTTAAAGTTATCATATCAAAATAATG
ACCGTGCAGTAGCAAGTTTAGCAACTAATGGATTGATAAAGGTAGCGATAAATAAAAAAGGCTATATCCTAGGAGCGACA
ATTGTAGGTGAGAATGCCAGCGAACTAATTGTACAATGGACAATAGCTATTAAAAATAAGTTAAAAATCAAAAATATGGC
ATCACACATAGTCGCCTATCCAACTCTAAGTGAGTTAAATAAAAGATTGGCTGGTAACTACTTTATACCAGTATTGTATA
GCAATAAAGTTAGGAGCCTAGTTAGATTTTTAATAAAAATATTTGTTAAAAAACTATGA

Upstream 100 bases:

>100_bases
TTATATTAGAACCACAATTTATTGTACCAATAGTTGCTTTGGCGATATTATCAGTCGTCCCTGTATTCATCAAAAAATTT
AATAAAAAGGCTTATTAATT

Downstream 100 bases:

>100_bases
TAGAGCAGAAAATTAGACCAGTTTTTCAGAGAATATTTGTTGATGGTATTGCTAAGTTTATTGCGCCTGTGATAGCACCT
AATTTAATCACACTAATATC

Product: mercuric reductase protein

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIKADICIIGGGSGGLSVAAGAVQMGAKVVLCEGNKMGGDCLNYGCVPSKAIIEASRVIAKVNKAQAFGINIDNNNIEID
YKKVQEHIKITIAKIEPHDSVERFETLGVNVIQEYAQIIDQYTVKAGDNFIKARYIVIATGSRAAIPKIKGLAEVNYLTN
ETIFDLKEKPEHLMIVGGGPIGVELAQAYALLGSKVTIFEASDTILGVLDNDCRKIILKEFDRLGISIITNVNISEIAQD
DQEINLYCGSKLYQGSHLLIAAGRVPNLDKLDLDNVGIKYTSRGIKVDSRLRTNHKNIYAIGDVVGGYQFTHVAGYHAGV
VIQNILFKLPIKVDYNSLPWSLYTSPEVAHVGQNIAQTQTHGAKILKLSYQNNDRAVASLATNGLIKVAINKKGYILGAT
IVGENASELIVQWTIAIKNKLKIKNMASHIVAYPTLSELNKRLAGNYFIPVLYSNKVRSLVRFLIKIFVKKL

Sequences:

>Translated_472_residues
MIKADICIIGGGSGGLSVAAGAVQMGAKVVLCEGNKMGGDCLNYGCVPSKAIIEASRVIAKVNKAQAFGINIDNNNIEID
YKKVQEHIKITIAKIEPHDSVERFETLGVNVIQEYAQIIDQYTVKAGDNFIKARYIVIATGSRAAIPKIKGLAEVNYLTN
ETIFDLKEKPEHLMIVGGGPIGVELAQAYALLGSKVTIFEASDTILGVLDNDCRKIILKEFDRLGISIITNVNISEIAQD
DQEINLYCGSKLYQGSHLLIAAGRVPNLDKLDLDNVGIKYTSRGIKVDSRLRTNHKNIYAIGDVVGGYQFTHVAGYHAGV
VIQNILFKLPIKVDYNSLPWSLYTSPEVAHVGQNIAQTQTHGAKILKLSYQNNDRAVASLATNGLIKVAINKKGYILGAT
IVGENASELIVQWTIAIKNKLKIKNMASHIVAYPTLSELNKRLAGNYFIPVLYSNKVRSLVRFLIKIFVKKL
>Mature_472_residues
MIKADICIIGGGSGGLSVAAGAVQMGAKVVLCEGNKMGGDCLNYGCVPSKAIIEASRVIAKVNKAQAFGINIDNNNIEID
YKKVQEHIKITIAKIEPHDSVERFETLGVNVIQEYAQIIDQYTVKAGDNFIKARYIVIATGSRAAIPKIKGLAEVNYLTN
ETIFDLKEKPEHLMIVGGGPIGVELAQAYALLGSKVTIFEASDTILGVLDNDCRKIILKEFDRLGISIITNVNISEIAQD
DQEINLYCGSKLYQGSHLLIAAGRVPNLDKLDLDNVGIKYTSRGIKVDSRLRTNHKNIYAIGDVVGGYQFTHVAGYHAGV
VIQNILFKLPIKVDYNSLPWSLYTSPEVAHVGQNIAQTQTHGAKILKLSYQNNDRAVASLATNGLIKVAINKKGYILGAT
IVGENASELIVQWTIAIKNKLKIKNMASHIVAYPTLSELNKRLAGNYFIPVLYSNKVRSLVRFLIKIFVKKL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=456, Percent_Identity=31.359649122807, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=26.4770240700219, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=25.1101321585903, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=23.5042735042735, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=23.5042735042735, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=23.5042735042735, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=23.5042735042735, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=23.5042735042735, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=22.1491228070175, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI291045268, Length=448, Percent_Identity=21.875, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=456, Percent_Identity=30.0438596491228, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=29.3216630196937, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=27.6824034334764, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=30.8370044052863, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17557007, Length=468, Percent_Identity=25.8547008547009, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71983419, Length=344, Percent_Identity=26.7441860465116, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983429, Length=344, Percent_Identity=26.7441860465116, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71982272, Length=436, Percent_Identity=23.394495412844, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=469, Percent_Identity=31.3432835820896, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=28.051391862955, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=25.4777070063694, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358499, Length=454, Percent_Identity=32.15859030837, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=28.8702928870293, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=28.8702928870293, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=28.8702928870293, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17737741, Length=478, Percent_Identity=26.5690376569038, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51536; Mature: 51536

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKADICIIGGGSGGLSVAAGAVQMGAKVVLCEGNKMGGDCLNYGCVPSKAIIEASRVIA
CEEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCHHCCCCCCCCHHHHHHHHHHH
KVNKAQAFGINIDNNNIEIDYKKVQEHIKITIAKIEPHDSVERFETLGVNVIQEYAQIID
HHCCCEEEEEEECCCEEEEEHHHHCCEEEEEEEEECCCCHHHHHHHHCHHHHHHHHHHHH
QYTVKAGDNFIKARYIVIATGSRAAIPKIKGLAEVNYLTNETIFDLKEKPEHLMIVGGGP
HHHHCCCCCEEEEEEEEEECCCCCCCCHHCCCEEEEEECCCEEEECCCCCCEEEEECCCC
IGVELAQAYALLGSKVTIFEASDTILGVLDNDCRKIILKEFDRLGISIITNVNISEIAQD
CHHHHHHHHHHHCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCEEEEECCCHHHHHCC
DQEINLYCGSKLYQGSHLLIAAGRVPNLDKLDLDNVGIKYTSRGIKVDSRLRTNHKNIYA
CCEEEEEECCEEECCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEECHHHHCCCCEEEE
IGDVVGGYQFTHVAGYHAGVVIQNILFKLPIKVDYNSLPWSLYTSPEVAHVGQNIAQTQT
EECCCCCEEEEEECCCHHHHHHHHHHHHCCEEEECCCCCEEEECCCCHHHHCCHHHHHHC
HGAKILKLSYQNNDRAVASLATNGLIKVAINKKGYILGATIVGENASELIVQWTIAIKNK
CCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEEEECCC
LKIKNMASHIVAYPTLSELNKRLAGNYFIPVLYSNKVRSLVRFLIKIFVKKL
EEHHHHHHHHEECCCHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKADICIIGGGSGGLSVAAGAVQMGAKVVLCEGNKMGGDCLNYGCVPSKAIIEASRVIA
CEEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCHHCCCCCCCCHHHHHHHHHHH
KVNKAQAFGINIDNNNIEIDYKKVQEHIKITIAKIEPHDSVERFETLGVNVIQEYAQIID
HHCCCEEEEEEECCCEEEEEHHHHCCEEEEEEEEECCCCHHHHHHHHCHHHHHHHHHHHH
QYTVKAGDNFIKARYIVIATGSRAAIPKIKGLAEVNYLTNETIFDLKEKPEHLMIVGGGP
HHHHCCCCCEEEEEEEEEECCCCCCCCHHCCCEEEEEECCCEEEECCCCCCEEEEECCCC
IGVELAQAYALLGSKVTIFEASDTILGVLDNDCRKIILKEFDRLGISIITNVNISEIAQD
CHHHHHHHHHHHCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCEEEEECCCHHHHHCC
DQEINLYCGSKLYQGSHLLIAAGRVPNLDKLDLDNVGIKYTSRGIKVDSRLRTNHKNIYA
CCEEEEEECCEEECCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEECHHHHCCCCEEEE
IGDVVGGYQFTHVAGYHAGVVIQNILFKLPIKVDYNSLPWSLYTSPEVAHVGQNIAQTQT
EECCCCCEEEEEECCCHHHHHHHHHHHHCCEEEECCCCCEEEECCCCHHHHCCHHHHHHC
HGAKILKLSYQNNDRAVASLATNGLIKVAINKKGYILGATIVGENASELIVQWTIAIKNK
CCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEEEECCC
LKIKNMASHIVAYPTLSELNKRLAGNYFIPVLYSNKVRSLVRFLIKIFVKKL
EEHHHHHHHHEECCCHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]