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The map label for this gene is def [H]

Identifier: 89255517

GI number: 89255517

Start: 71761

End: 72279

Strand: Reverse

Name: def [H]

Synonym: FTL_0074

Alternate gene names: 89255517

Gene position: 72279-71761 (Counterclockwise)

Preceding gene: 89255518

Following gene: 89255516

Centisome position: 3.81

GC content: 33.33

Gene sequence:

>519_bases
ATGTCTTTAGAAATTCTAAAGTACCCTCACCCAGTTTTAAAAGAGGTTGCTAAAGAAGTTACAAAAGATGAAATCAATGA
TGATTTACGTGCAACTATTGCTGAAATGCATGAGCTAATGCTAGAAGCAAATGGCGTGGGTTTAGCAGCAATACAAGTTG
GTATCAAAAAAAGATTCTTTATCATGTATGATAACTTAGAAGAGCAAAACCCTGAAATAATCACTATCATTAACCCTGAA
ATAATTGAACAAAACGGTAAAATAATTGATGAAGAGGGCTGTCTTTCTTTTCCTGGTGTTTCCGCAAAGGTAAATAGGGC
CACTGTAGTCAAAATAAAGGCACTTAATGAATTTGGAGAAGAGATTGAAGTTGAAAAAGATGGATTTTTAGCTAGATGCA
TCCAACATGAGATAGATCATCTTAATGGTATAACTTTCTTTGACCATCTTGGTTCACTAAAGCGCAAAATGATAGAGAAA
AAGTATAAAAAACTAATGCAAGAAAATGCTAAGAGTTAA

Upstream 100 bases:

>100_bases
GAGTCGGTGGCAAAAAAGGTCATAAAGGTAAATACTCTATCCAAGCTGCCATAACGATGGCAAAGATGAAAAAAGATATT
TTAGAACAAGGAGTATGAAT

Downstream 100 bases:

>100_bases
TTTTTGATTATTTTGTATAATCAATACTTTACTTTTTAGCAGCATTACGCAAAAATTATCTCCTGTAATTGTTATTAAAT
CATGGAATTTAGTATGCGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFFIMYDNLEEQNPEIITIINPE
IIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEK
KYKKLMQENAKS

Sequences:

>Translated_172_residues
MSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFFIMYDNLEEQNPEIITIINPE
IIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEK
KYKKLMQENAKS
>Mature_171_residues
SLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFFIMYDNLEEQNPEIITIINPEI
IEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGEEIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEKK
YKKLMQENAKS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=42.3529411764706, Blast_Score=129, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=36.0759493670886, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19579; Mature: 19448

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFF
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEE
IMYDNLEEQNPEIITIINPEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGE
EEECCCCCCCCCEEEEECHHHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHH
EIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEKKYKKLMQENAKS
HHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLEILKYPHPVLKEVAKEVTKDEINDDLRATIAEMHELMLEANGVGLAAIQVGIKKRFF
CCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEE
IMYDNLEEQNPEIITIINPEIIEQNGKIIDEEGCLSFPGVSAKVNRATVVKIKALNEFGE
EEECCCCCCCCCEEEEECHHHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHH
EIEVEKDGFLARCIQHEIDHLNGITFFDHLGSLKRKMIEKKYKKLMQENAKS
HHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA