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The map label for this gene is bcr [H]

Identifier: 89255510

GI number: 89255510

Start: 62556

End: 63692

Strand: Direct

Name: bcr [H]

Synonym: FTL_0063

Alternate gene names: 89255510

Gene position: 62556-63692 (Clockwise)

Preceding gene: 89255507

Following gene: 89255511

Centisome position: 3.3

GC content: 30.52

Gene sequence:

>1137_bases
ATGAAAACTTACAAAATTATAATTTTACTAACTTACATAAGTATAGCTAGTGCTTCTGCTGTAATTATAAACCCAGCTTT
ACCTAATATTGCTACAGAGCTTGGTTTGTCGAGTGGTACGGTTGAATGGTTGGTAAGTATATTCTTATTAGGCTATGTGC
TAGGTCAGATAATATATGGACCAATTGCAAAGAAATATGGAGATGTCATAACCCTAAGGGCAGGGATGACTATAAATGTT
ATTGGTATACTAGTTTGTATACTAGCCTCTTCTATGACAATGTTGCTTATAGGAAGATTTATAACGGCTATAGGAGCATC
GGCAGGGTTGGTGTGTACTTTTATTATATTAAATAATTCGGTTGCTGCTGAGAAAGCTAAATTAGCTTTATCTTTTGCTA
TATCGCTAACGCTAGCTACCTTAATAGGTGGAATCATTAGTGCTTATACTCATTGGTATTATTGTTTTTATGTACTATTG
ATACATGCTGTAATAATGTTAGGTGCTAGTTTCCTATATGAAAATAAAAAAGACTTTGAATTTAATTTAAAGGTATCAGC
TATTATTGATGGTTATAAAAATGCTTTTAGTAGTTTTAAATTAGTAGTATTTGCATTGACGCTAGGTGTTATGACTGTTT
TTAGTTATTGTTATTCTGCAGCTGGACCTTTTATAGCTCATAGTATGTTTAATCTAAACAGTACTCAGTATAGTTTGTGG
ACTTCGATGACTATTATAGGCATTGTATTAGGTTCAATGTTAGTTGCTAAAATAATAAATAAACATGACACTCATAAGAT
ACTTATTGTGGCATTAATTTGTTTTGTTGTGTTAGTTGTAGTTATGGCATGGTTAAAAATAGCTAATTATATGACACCGT
TAATATTTTTTGTATTAATAACTTTGATGTATTTTGTATGTAATTTTATATACCCTACAGCATCATACCTTGCATCAACT
GCAATTGAATGTAAGTCAAACGCATCTGCAGCGATGAACTTTATAAATATGCTAACAGCTGTTGTAGCAGTTTCTATAAT
GGGTTATATACCGTTTGAATACATTTGGAACTTTTTGTTGATGTGTACAATACTACCAATTATTTGTTTTGTACTAATAA
TCTATAATAAATTTTAA

Upstream 100 bases:

>100_bases
TCAAATAAATCTAAACTTATGTCAATATATGGTGATATTGTAAAATATATTTTTAAATTTACCATATCTCATAGTTAAAT
TTTTATGAAGAGGATTAATT

Downstream 100 bases:

>100_bases
AAAATAATAATCTTTACAAATATTTTGTTAAATTGATTCTTAAGAAACTAAGTTTAGTATTTGTACTTATTCAAAGCTAG
TGGCAGTAAAACGTTGAACT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYGPIAKKYGDVITLRAGMTINV
IGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNSVAAEKAKLALSFAISLTLATLIGGIISAYTHWYYCFYVLL
IHAVIMLGASFLYENKKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNSTQYSLW
TSMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLITLMYFVCNFIYPTASYLAST
AIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLLMCTILPIICFVLIIYNKF

Sequences:

>Translated_378_residues
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYGPIAKKYGDVITLRAGMTINV
IGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNSVAAEKAKLALSFAISLTLATLIGGIISAYTHWYYCFYVLL
IHAVIMLGASFLYENKKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNSTQYSLW
TSMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLITLMYFVCNFIYPTASYLAST
AIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLLMCTILPIICFVLIIYNKF
>Mature_378_residues
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYGPIAKKYGDVITLRAGMTINV
IGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNSVAAEKAKLALSFAISLTLATLIGGIISAYTHWYYCFYVLL
IHAVIMLGASFLYENKKDFEFNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNSTQYSLW
TSMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLITLMYFVCNFIYPTASYLAST
AIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLLMCTILPIICFVLIIYNKF

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=213, Percent_Identity=23.943661971831, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41535; Mature: 41535

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYG
CCCEEEEHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
PIAKKYGDVITLRAGMTINVIGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNS
HHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
VAAEKAKLALSFAISLTLATLIGGIISAYTHWYYCFYVLLIHAVIMLGASFLYENKKDFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNSTQYSLW
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHH
TSMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLMYFVCNFIYPTASYLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MCTILPIICFVLIIYNKF
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTYKIIILLTYISIASASAVIINPALPNIATELGLSSGTVEWLVSIFLLGYVLGQIIYG
CCCEEEEHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
PIAKKYGDVITLRAGMTINVIGILVCILASSMTMLLIGRFITAIGASAGLVCTFIILNNS
HHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCC
VAAEKAKLALSFAISLTLATLIGGIISAYTHWYYCFYVLLIHAVIMLGASFLYENKKDFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FNLKVSAIIDGYKNAFSSFKLVVFALTLGVMTVFSYCYSAAGPFIAHSMFNLNSTQYSLW
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHH
TSMTIIGIVLGSMLVAKIINKHDTHKILIVALICFVVLVVVMAWLKIANYMTPLIFFVLI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLMYFVCNFIYPTASYLASTAIECKSNASAAMNFINMLTAVVAVSIMGYIPFEYIWNFLL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
MCTILPIICFVLIIYNKF
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]