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The map label for this gene is pyrB [H]

Identifier: 89255475

GI number: 89255475

Start: 26372

End: 27292

Strand: Reverse

Name: pyrB [H]

Synonym: FTL_0028

Alternate gene names: 89255475

Gene position: 27292-26372 (Counterclockwise)

Preceding gene: 89255476

Following gene: 89255472

Centisome position: 1.44

GC content: 32.68

Gene sequence:

>921_bases
ATGCTATCATTTCAAAAGTTACTACGCGGTGATCAAATTATTAAAGATGATTTGTTTAAACTATTTCATCAAGCTGATAT
TTATAAAAATAAACTAGCTAATTCAGAGCCTATCAAAGATTTAGAAGGCAAAATATTAGCATCTCTTTTCTTTGAACCAA
GCACTAGAACTAGATTCTCTTTTGAATCTGCTATGAATAGACTTGGTGGTAAAGTAATATCACTAGAAAATGGCTCATCA
AGCTCAACACAAAAAGGTGAAACCCTTGATGATACAGGTAGAATAATTGATCAATATGCTGATATTATCGTGATGCGTCA
CCCTAAACCACATAGTGTTGAAGAGTTCTCTAAATATGTCAACTCTCCTGTGATAAATGCTGGCGATGGTGAAAATGAAC
ATCCCACTCAGTCATTGGTTGACTTATATACTATCTACTTTGAGAAAGGCTCTCTTGATAATCTAAAAGTTGGTTTCTTA
GGAGATCTAAAATATGGTCGTACGGTTCACTCACTAACCAATATCCTTGCTAAATATAATGCCTCGTTTAAATTTATATC
TTCAAAAGAGCTACAGATTCCTAAGAAACTAAAAGATTTTATCATCAAAAATGGCAACTCTTTTATTGAGTTAGATAAAG
TTAGTGCCGATGTTTTAGAAGACTTAGATGTACTATATGTAACTCGTGTCCAGAGAGAAAGGTTTCCTGATGAAGAAAGC
TATCTAAAAAATAAAGATTTATGCTATTTAGATAGAAAGCATATTTCCCCTAGAAGTAATTTTATAATTCTTCATCCTCT
ACCTAGAGTTGATGAGATGGATAGATCTATTGATGAGCTTGAGTGTGCGAAATATTTTGAGCAAATCAAATATAGTATTC
CGATTAGGATGGCGCTATTGGCTTTATTAGTAATATCCTAA

Upstream 100 bases:

>100_bases
ACCATAGGATCGATTATCTTTTTAAAACTACCCCGTCAATCTAACGACTACCACCCCTTCGTACCAAAGGGGAATAAATA
GAACGTTTTAGGAGTAAAAA

Downstream 100 bases:

>100_bases
AAAATAAAATTTGTTATTTTTTATAAATTTCTTTATAAGCTCTATATAACCCATAAAAATCTTGATTTGTAAAATCATGT
TTAATAGCATATTCAACTAC

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLSFQKLLRGDQIIKDDLFKLFHQADIYKNKLANSEPIKDLEGKILASLFFEPSTRTRFSFESAMNRLGGKVISLENGSS
SSTQKGETLDDTGRIIDQYADIIVMRHPKPHSVEEFSKYVNSPVINAGDGENEHPTQSLVDLYTIYFEKGSLDNLKVGFL
GDLKYGRTVHSLTNILAKYNASFKFISSKELQIPKKLKDFIIKNGNSFIELDKVSADVLEDLDVLYVTRVQRERFPDEES
YLKNKDLCYLDRKHISPRSNFIILHPLPRVDEMDRSIDELECAKYFEQIKYSIPIRMALLALLVIS

Sequences:

>Translated_306_residues
MLSFQKLLRGDQIIKDDLFKLFHQADIYKNKLANSEPIKDLEGKILASLFFEPSTRTRFSFESAMNRLGGKVISLENGSS
SSTQKGETLDDTGRIIDQYADIIVMRHPKPHSVEEFSKYVNSPVINAGDGENEHPTQSLVDLYTIYFEKGSLDNLKVGFL
GDLKYGRTVHSLTNILAKYNASFKFISSKELQIPKKLKDFIIKNGNSFIELDKVSADVLEDLDVLYVTRVQRERFPDEES
YLKNKDLCYLDRKHISPRSNFIILHPLPRVDEMDRSIDELECAKYFEQIKYSIPIRMALLALLVIS
>Mature_306_residues
MLSFQKLLRGDQIIKDDLFKLFHQADIYKNKLANSEPIKDLEGKILASLFFEPSTRTRFSFESAMNRLGGKVISLENGSS
SSTQKGETLDDTGRIIDQYADIIVMRHPKPHSVEEFSKYVNSPVINAGDGENEHPTQSLVDLYTIYFEKGSLDNLKVGFL
GDLKYGRTVHSLTNILAKYNASFKFISSKELQIPKKLKDFIIKNGNSFIELDKVSADVLEDLDVLYVTRVQRERFPDEES
YLKNKDLCYLDRKHISPRSNFIILHPLPRVDEMDRSIDELECAKYFEQIKYSIPIRMALLALLVIS

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=39.672131147541, Blast_Score=239, Evalue=3e-63,
Organism=Escherichia coli, GI2367364, Length=296, Percent_Identity=44.5945945945946, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI193204318, Length=309, Percent_Identity=41.1003236245955, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322331, Length=301, Percent_Identity=41.1960132890365, Blast_Score=235, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=42.9508196721311, Blast_Score=248, Evalue=2e-66,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 35098; Mature: 35098

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFQKLLRGDQIIKDDLFKLFHQADIYKNKLANSEPIKDLEGKILASLFFEPSTRTRFS
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
FESAMNRLGGKVISLENGSSSSTQKGETLDDTGRIIDQYADIIVMRHPKPHSVEEFSKYV
HHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHH
NSPVINAGDGENEHPTQSLVDLYTIYFEKGSLDNLKVGFLGDLKYGRTVHSLTNILAKYN
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
ASFKFISSKELQIPKKLKDFIIKNGNSFIELDKVSADVLEDLDVLYVTRVQRERFPDEES
CCEEEECCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
YLKNKDLCYLDRKHISPRSNFIILHPLPRVDEMDRSIDELECAKYFEQIKYSIPIRMALL
HHCCCCCEEEHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALLVIS
HHHHCC
>Mature Secondary Structure
MLSFQKLLRGDQIIKDDLFKLFHQADIYKNKLANSEPIKDLEGKILASLFFEPSTRTRFS
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
FESAMNRLGGKVISLENGSSSSTQKGETLDDTGRIIDQYADIIVMRHPKPHSVEEFSKYV
HHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHHHH
NSPVINAGDGENEHPTQSLVDLYTIYFEKGSLDNLKVGFLGDLKYGRTVHSLTNILAKYN
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHC
ASFKFISSKELQIPKKLKDFIIKNGNSFIELDKVSADVLEDLDVLYVTRVQRERFPDEES
CCEEEECCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
YLKNKDLCYLDRKHISPRSNFIILHPLPRVDEMDRSIDELECAKYFEQIKYSIPIRMALL
HHCCCCCEEEHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALLVIS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9209027; 12622808 [H]