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The map label for this gene is proP [H]

Identifier: 89255469

GI number: 89255469

Start: 19555

End: 20736

Strand: Reverse

Name: proP [H]

Synonym: FTL_0021

Alternate gene names: 89255469

Gene position: 20736-19555 (Counterclockwise)

Preceding gene: 89255471

Following gene: 89255468

Centisome position: 1.09

GC content: 34.09

Gene sequence:

>1182_bases
TTGCGTATTAAGTCAGTAATTATCTCGAGTATTGGCAGTACTTTTGAGACTTTTGATTTTCATATATTTGGTTTATTTGC
TTTGCAGATATCAATGTCGTTATTGCATAAAGATTCTTTTAATAGCTCTCTTATCTTGATTTTTGCTATTTTCGGCGCGG
GTTATTTTGCTAGATCTCTGGGAGCTATATTTTGGGGTAATCTTGGCGATAAATATGGTCGTACCGTATCGTTTAAGTGG
ACGGTTATTCTAATAGGTGTAGCATCTTTAGTAATAGCGGTATTGCCAAGTAGTAGTGTTATTGGTGTATTTTCGCCATT
GATATTAGCAGCTTGTCGTTTAATTCAAGGTTTTTCATTCGGAGGTGAGCTAATTGCTGCAGTTTTATTAGTCTATGAGC
AGCCAGCAAAAAGTAGAAGTTTCTATACTTCTGTAGTTATTTTTTGTGCAACTTTAGGGATGATTTTAGCGTCAATAGTT
TACTATCTTCTAAGCCTTGTAATTACTACAGAGCAGTTTTTTGCCTTTAGCTGGCGTTTGGCATTTGCTTTTGGTGGTAT
TGCTATATTAGTGAGCTATTTTTGGCGTGCTTCAATTCAAGAAACATTAGTTACTGAGTCTACTGGTAAGCGTGGCTTTG
TTGCTTTAGAGCTACTAAAGACCAATATTAGATTACTCTTGCGTTGCGTTTTAACTGCTGCTGCAACAGCGATATTTCTA
TCAATGTTTATAGTATTTTTACCAACATTTTGCCAAAAGGGCTTGGGTTTTGATAAGGTTACAACTTCTGTGTATGTTTT
AGTAACTATAGTAACTTATGCAGTCTCAGCATTAGCTGGTGGCTACCTTGGTGATATTATTGGTAACAAAAGGACACAAG
CATTAGGTATAATTTTGGTGATAATTATACTTATTCCACTATCTATATCATTATTAACAAAGTCTGCTGTGGCATTACTA
TTTATAGTGTTATTTGCAATTGTGAATGGTTTGATAAATGCAAACTATATGATTGTTGTCTTAAATAGATTTGATAAATT
ACAGAGATTTAGTGTCTTAGCTATTACACAGAATATTTCAATGACTATATTTATGGGAGTTTTACCTGCGCTATTTGCCT
TTTTGGTAGCTGATTTGAAAATCCTCTCAGCACCATTTTATATTTGTTTGACTAGAGTTTGA

Upstream 100 bases:

>100_bases
AGGGATCTGATGATGGTATTTATGAGTTTCTTGAAGCTAAGTATAGTTTGCAGGTTTTTGGTTAGTTAATTAAATTAATA
AATTTATTTAAGGAATTATC

Downstream 100 bases:

>100_bases
TAGTTTTTATTTGTATCATCAGTATTTTAGCTTAGCTATTATAGCGTTAACTTAATTTGTTTGCTTGGTTATTAATATTT
GTGCTTAGTCTTCTTTGTCA

Product: MFS superfamily proline/ betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRIKSVIISSIGSTFETFDFHIFGLFALQISMSLLHKDSFNSSLILIFAIFGAGYFARSLGAIFWGNLGDKYGRTVSFKW
TVILIGVASLVIAVLPSSSVIGVFSPLILAACRLIQGFSFGGELIAAVLLVYEQPAKSRSFYTSVVIFCATLGMILASIV
YYLLSLVITTEQFFAFSWRLAFAFGGIAILVSYFWRASIQETLVTESTGKRGFVALELLKTNIRLLLRCVLTAAATAIFL
SMFIVFLPTFCQKGLGFDKVTTSVYVLVTIVTYAVSALAGGYLGDIIGNKRTQALGIILVIIILIPLSISLLTKSAVALL
FIVLFAIVNGLINANYMIVVLNRFDKLQRFSVLAITQNISMTIFMGVLPALFAFLVADLKILSAPFYICLTRV

Sequences:

>Translated_393_residues
MRIKSVIISSIGSTFETFDFHIFGLFALQISMSLLHKDSFNSSLILIFAIFGAGYFARSLGAIFWGNLGDKYGRTVSFKW
TVILIGVASLVIAVLPSSSVIGVFSPLILAACRLIQGFSFGGELIAAVLLVYEQPAKSRSFYTSVVIFCATLGMILASIV
YYLLSLVITTEQFFAFSWRLAFAFGGIAILVSYFWRASIQETLVTESTGKRGFVALELLKTNIRLLLRCVLTAAATAIFL
SMFIVFLPTFCQKGLGFDKVTTSVYVLVTIVTYAVSALAGGYLGDIIGNKRTQALGIILVIIILIPLSISLLTKSAVALL
FIVLFAIVNGLINANYMIVVLNRFDKLQRFSVLAITQNISMTIFMGVLPALFAFLVADLKILSAPFYICLTRV
>Mature_393_residues
MRIKSVIISSIGSTFETFDFHIFGLFALQISMSLLHKDSFNSSLILIFAIFGAGYFARSLGAIFWGNLGDKYGRTVSFKW
TVILIGVASLVIAVLPSSSVIGVFSPLILAACRLIQGFSFGGELIAAVLLVYEQPAKSRSFYTSVVIFCATLGMILASIV
YYLLSLVITTEQFFAFSWRLAFAFGGIAILVSYFWRASIQETLVTESTGKRGFVALELLKTNIRLLLRCVLTAAATAIFL
SMFIVFLPTFCQKGLGFDKVTTSVYVLVTIVTYAVSALAGGYLGDIIGNKRTQALGIILVIIILIPLSISLLTKSAVALL
FIVLFAIVNGLINANYMIVVLNRFDKLQRFSVLAITQNISMTIFMGVLPALFAFLVADLKILSAPFYICLTRV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=400, Percent_Identity=25.5, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788292, Length=393, Percent_Identity=26.4631043256997, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1789941, Length=313, Percent_Identity=28.4345047923323, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788942, Length=345, Percent_Identity=25.2173913043478, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 42920; Mature: 42920

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIKSVIISSIGSTFETFDFHIFGLFALQISMSLLHKDSFNSSLILIFAIFGAGYFARSL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
GAIFWGNLGDKYGRTVSFKWTVILIGVASLVIAVLPSSSVIGVFSPLILAACRLIQGFSF
HHHHHCCCCHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
GGELIAAVLLVYEQPAKSRSFYTSVVIFCATLGMILASIVYYLLSLVITTEQFFAFSWRL
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAFGGIAILVSYFWRASIQETLVTESTGKRGFVALELLKTNIRLLLRCVLTAAATAIFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMFIVFLPTFCQKGLGFDKVTTSVYVLVTIVTYAVSALAGGYLGDIIGNKRTQALGIILV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH
IIILIPLSISLLTKSAVALLFIVLFAIVNGLINANYMIVVLNRFDKLQRFSVLAITQNIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHEEEECCCH
MTIFMGVLPALFAFLVADLKILSAPFYICLTRV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECC
>Mature Secondary Structure
MRIKSVIISSIGSTFETFDFHIFGLFALQISMSLLHKDSFNSSLILIFAIFGAGYFARSL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
GAIFWGNLGDKYGRTVSFKWTVILIGVASLVIAVLPSSSVIGVFSPLILAACRLIQGFSF
HHHHHCCCCHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
GGELIAAVLLVYEQPAKSRSFYTSVVIFCATLGMILASIVYYLLSLVITTEQFFAFSWRL
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFAFGGIAILVSYFWRASIQETLVTESTGKRGFVALELLKTNIRLLLRCVLTAAATAIFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMFIVFLPTFCQKGLGFDKVTTSVYVLVTIVTYAVSALAGGYLGDIIGNKRTQALGIILV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH
IIILIPLSISLLTKSAVALLFIVLFAIVNGLINANYMIVVLNRFDKLQRFSVLAITQNIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHEEEECCCH
MTIFMGVLPALFAFLVADLKILSAPFYICLTRV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]