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The map label for this gene is amtB [C]

Identifier: 89056548

GI number: 89056548

Start: 4161692

End: 4162999

Strand: Direct

Name: amtB [C]

Synonym: Jann_4057

Alternate gene names: 89056548

Gene position: 4161692-4162999 (Clockwise)

Preceding gene: 89056547

Following gene: 89056549

Centisome position: 96.38

GC content: 62.69

Gene sequence:

>1308_bases
ATGCAAAATAAGTATCTTCTTCCACTCTTGGCGGCTGCGACCATCGCGCTTCCAACCGTGGCGGCGGCCCAGGATGAGGC
CGCACTGCACACCTCCTACATCCTCACCACGCTGCTGTTTCTTGTCGGTGGCTTTCTGGTGTTCTGGATGGCGGCGGGCT
TCGCGATGCTGGAGGCCGGTCTTGTCCGCTCCAAGAACGTCGCGATGCAGCTGACCAAGAACATCGCGCTCTTTTCCATC
GCGGCGATCATGTATTGGCTGGTGGGGTTTGGCATCATGTACCCCGCCGATTGGCTGGTCGAAGGCTGGCTGGGGCCGTT
CTTCGCCATCACGTCGCTGGAGCCTGTGGGCCTCGCCGATACGGAAACCGCGCTGGATTATGCCTCCGTCGGCTCCGACT
TCTTCTTCCAGCTGATGTTCTGCGCCACCACAGCCTCCATCGTGTCGGGCACATTGGCCGAGCGGATCAAGCTCTGGCCC
TTCCTGATTTTCGTGGTCGTGCTGACCGGCTTCATCTATCCCATCGAGGCCTCCTGGCAGTGGGGCGGCGGCTGGTTGTC
TGAAGCGGGCTTCTCCGACTTCGCAGGCTCCACGCTCGTTCACGCCGCTGGTGGCTTCGCCGCGTTGGCCGGTGCCATCG
TGCTGGGTCCCCGTCTGGGCAAGTACAAGGATGGTCGCGTCAACCCGATGCCCGGCTCGAACCTTGCCTTGGCGACCTTG
GGTACGTTCATCCTGTGGCTCGGCTGGTTCGGCTTCAACGGCGGCTCGCAGCTGGCGATGGGCACAATCGGCGACGTCGC
GGACGTCTCCCGCATCTTCGCCAACACCAACATGGCCGCCGCCTCCGGTGCGGTTGCTGCACTCATTCTGACGCAGGTCA
TGTACGGCAAGGTCGACCTGACGATGGTGTTGAACGGTGCCCTGGCGGGCCTTGTGTCGATCACGGCTGAACCTTTGGCG
CCGTCGCTTTTCGGCGCGCTACTGACCGGCGCGGTCGGTGGCCTGATCGTCGTCTTCACGGTGCCGCTGCTGGACAAGCT
GAAGATCGACGACGTCGTCGGTGCCATCCCGGTTCACCTCTTTGCAGGGGTCTGGGGCACGCTTGCGGTGGCCTTCTACA
CCGGCAATTGGGGCGCACAGATCCTTGGCATCGCAGCCATCGGCATCTTCGTTTTCGTCACCTCGCTGGTGGTCTGGTTC
ATCCTCAAGGCGACCGTGGGCATCCGCGTCGGCGAGGAGGAGGAGATCAACGGCCTCGACGTGGCGGAATTGGGAATGGA
AGCCTACCCCGAGTTCTCCAAGGGCTAA

Upstream 100 bases:

>100_bases
CAAGATCTTCGTGCTCGACGTGCAAAGCGCTGTGCGCGTGCGGACCGGCGAAACCAACGATGACGCGCTTTAAGTGTTGA
TGGGTAAGGGAACCAAACCT

Downstream 100 bases:

>100_bases
GCCCCACCCAAAAACCTTTGAAGGGGGCGTCCATCACGGGCGCCCCTTTTGTCATCGCGGGTGTCTGATTAGGCTAGATC
CCATGCAATCTGAAGCCCCA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQNKYLLPLLAAATIALPTVAAAQDEAALHTSYILTTLLFLVGGFLVFWMAAGFAMLEAGLVRSKNVAMQLTKNIALFSI
AAIMYWLVGFGIMYPADWLVEGWLGPFFAITSLEPVGLADTETALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWP
FLIFVVVLTGFIYPIEASWQWGGGWLSEAGFSDFAGSTLVHAAGGFAALAGAIVLGPRLGKYKDGRVNPMPGSNLALATL
GTFILWLGWFGFNGGSQLAMGTIGDVADVSRIFANTNMAAASGAVAALILTQVMYGKVDLTMVLNGALAGLVSITAEPLA
PSLFGALLTGAVGGLIVVFTVPLLDKLKIDDVVGAIPVHLFAGVWGTLAVAFYTGNWGAQILGIAAIGIFVFVTSLVVWF
ILKATVGIRVGEEEEINGLDVAELGMEAYPEFSKG

Sequences:

>Translated_435_residues
MQNKYLLPLLAAATIALPTVAAAQDEAALHTSYILTTLLFLVGGFLVFWMAAGFAMLEAGLVRSKNVAMQLTKNIALFSI
AAIMYWLVGFGIMYPADWLVEGWLGPFFAITSLEPVGLADTETALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWP
FLIFVVVLTGFIYPIEASWQWGGGWLSEAGFSDFAGSTLVHAAGGFAALAGAIVLGPRLGKYKDGRVNPMPGSNLALATL
GTFILWLGWFGFNGGSQLAMGTIGDVADVSRIFANTNMAAASGAVAALILTQVMYGKVDLTMVLNGALAGLVSITAEPLA
PSLFGALLTGAVGGLIVVFTVPLLDKLKIDDVVGAIPVHLFAGVWGTLAVAFYTGNWGAQILGIAAIGIFVFVTSLVVWF
ILKATVGIRVGEEEEINGLDVAELGMEAYPEFSKG
>Mature_435_residues
MQNKYLLPLLAAATIALPTVAAAQDEAALHTSYILTTLLFLVGGFLVFWMAAGFAMLEAGLVRSKNVAMQLTKNIALFSI
AAIMYWLVGFGIMYPADWLVEGWLGPFFAITSLEPVGLADTETALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWP
FLIFVVVLTGFIYPIEASWQWGGGWLSEAGFSDFAGSTLVHAAGGFAALAGAIVLGPRLGKYKDGRVNPMPGSNLALATL
GTFILWLGWFGFNGGSQLAMGTIGDVADVSRIFANTNMAAASGAVAALILTQVMYGKVDLTMVLNGALAGLVSITAEPLA
PSLFGALLTGAVGGLIVVFTVPLLDKLKIDDVVGAIPVHLFAGVWGTLAVAFYTGNWGAQILGIAAIGIFVFVTSLVVWF
ILKATVGIRVGEEEEINGLDVAELGMEAYPEFSKG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=442, Percent_Identity=31.6742081447964, Blast_Score=145, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32564042, Length=417, Percent_Identity=32.3741007194245, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17550180, Length=426, Percent_Identity=33.5680751173709, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17550182, Length=430, Percent_Identity=32.3255813953488, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI133931100, Length=423, Percent_Identity=28.3687943262411, Blast_Score=158, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6325396, Length=428, Percent_Identity=28.0373831775701, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=424, Percent_Identity=28.5377358490566, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=414, Percent_Identity=27.0531400966184, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI161078314, Length=440, Percent_Identity=32.5, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45782; Mature: 45782

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKYLLPLLAAATIALPTVAAAQDEAALHTSYILTTLLFLVGGFLVFWMAAGFAMLEAG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRSKNVAMQLTKNIALFSIAAIMYWLVGFGIMYPADWLVEGWLGPFFAITSLEPVGLAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCCCCCCC
TETALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWPFLIFVVVLTGFIYPIEASWQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
WGGGWLSEAGFSDFAGSTLVHAAGGFAALAGAIVLGPRLGKYKDGRVNPMPGSNLALATL
CCCCHHCCCCCCHHCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
GTFILWLGWFGFNGGSQLAMGTIGDVADVSRIFANTNMAAASGAVAALILTQVMYGKVDL
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHH
TMVLNGALAGLVSITAEPLAPSLFGALLTGAVGGLIVVFTVPLLDKLKIDDVVGAIPVHL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAGVWGTLAVAFYTGNWGAQILGIAAIGIFVFVTSLVVWFILKATVGIRVGEEEEINGLD
HHHHHHHHEEHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VAELGMEAYPEFSKG
HHHHCHHHCCCCCCC
>Mature Secondary Structure
MQNKYLLPLLAAATIALPTVAAAQDEAALHTSYILTTLLFLVGGFLVFWMAAGFAMLEAG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRSKNVAMQLTKNIALFSIAAIMYWLVGFGIMYPADWLVEGWLGPFFAITSLEPVGLAD
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHCCCCCCCCC
TETALDYASVGSDFFFQLMFCATTASIVSGTLAERIKLWPFLIFVVVLTGFIYPIEASWQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
WGGGWLSEAGFSDFAGSTLVHAAGGFAALAGAIVLGPRLGKYKDGRVNPMPGSNLALATL
CCCCHHCCCCCCHHCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
GTFILWLGWFGFNGGSQLAMGTIGDVADVSRIFANTNMAAASGAVAALILTQVMYGKVDL
HHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHH
TMVLNGALAGLVSITAEPLAPSLFGALLTGAVGGLIVVFTVPLLDKLKIDDVVGAIPVHL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAGVWGTLAVAFYTGNWGAQILGIAAIGIFVFVTSLVVWFILKATVGIRVGEEEEINGLD
HHHHHHHHEEHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VAELGMEAYPEFSKG
HHHHCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]