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The map label for this gene is pyrF

Identifier: 89056480

GI number: 89056480

Start: 4095453

End: 4096142

Strand: Direct

Name: pyrF

Synonym: Jann_3989

Alternate gene names: 89056480

Gene position: 4095453-4096142 (Clockwise)

Preceding gene: 89056477

Following gene: 89056484

Centisome position: 94.85

GC content: 66.23

Gene sequence:

>690_bases
ATGTCGGACGACCGTTTGATCGTGGCGCTGGATGTGCCCAACGTGGTGCAGGGCCTGGCGCTGGCCGAAAAACTCGGCGA
CGCCGTTTCATTCTACAAAATCGGACTGGGGATGCTGACCGGCGGTGGCCTGGCGCTCGCCAATGAGCTGAAGCAGGACC
ACGGCAAGCGCATTTTCCTTGATATGAAGTTGTTCGATATTTCCGCCACGGTCGAGGCCGCGGTGCGGGGGCTGGCGCAA
TTCAACCTGGATTTCCTGACCGTCCATGGCGATCCCCACGTGGTGCGCGCCGCCAAGGAAGGAGCGGCGGGCAGCGACAC
GAAGATCCTTGCGGTGACGATCCTGACCTCTCTGGATCGGACCGATCTGGACGATTGCCTGATCCGGGATGGAGAGATGC
TTGATCTGGTGGCCGAGCGGGCGGGCCGCGCGTTTGAGGCGGGCGCCGATGGCGTCATCTCTTCCCCGCACGAAGCGACC
ATGATCCGCGCCCTGCCCGAGGCCAAGGGCCGCCTGATCGTGACCCCCGGCGTCCGCCCAACGGGCAGCGCGTCCGGCGA
CCAGAAACGCATCGCGACGCCGCGCCAGGCCATCGCGGACGGGGCCGACCACATCGTCGTGGGCCGCCCGATCTGGCAGC
ATCAGGACCCGCGTGCGGCGGCGCAGATGGTGGTGGCGGACCTGCCTTAA

Upstream 100 bases:

>100_bases
TATCGCCCGTGGCCTGCGCGACGCCGCATTGAGCGTGGCCTAAGTGGACTTCCCCTCGGCCCGCGTCTAAGGAGCGCGAG
GTTCATGGGGGAGAGAAGAT

Downstream 100 bases:

>100_bases
TCGAAGGGCGGTTGCGGTCGATCCTCGGGCCACGGCGGCAGGGTGATCCCGTCCAGGGTCTCCGCATAGAACCCCATGGA
GCCGGGCATCCAGAACCGCG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSDDRLIVALDVPNVVQGLALAEKLGDAVSFYKIGLGMLTGGGLALANELKQDHGKRIFLDMKLFDISATVEAAVRGLAQ
FNLDFLTVHGDPHVVRAAKEGAAGSDTKILAVTILTSLDRTDLDDCLIRDGEMLDLVAERAGRAFEAGADGVISSPHEAT
MIRALPEAKGRLIVTPGVRPTGSASGDQKRIATPRQAIADGADHIVVGRPIWQHQDPRAAAQMVVADLP

Sequences:

>Translated_229_residues
MSDDRLIVALDVPNVVQGLALAEKLGDAVSFYKIGLGMLTGGGLALANELKQDHGKRIFLDMKLFDISATVEAAVRGLAQ
FNLDFLTVHGDPHVVRAAKEGAAGSDTKILAVTILTSLDRTDLDDCLIRDGEMLDLVAERAGRAFEAGADGVISSPHEAT
MIRALPEAKGRLIVTPGVRPTGSASGDQKRIATPRQAIADGADHIVVGRPIWQHQDPRAAAQMVVADLP
>Mature_228_residues
SDDRLIVALDVPNVVQGLALAEKLGDAVSFYKIGLGMLTGGGLALANELKQDHGKRIFLDMKLFDISATVEAAVRGLAQF
NLDFLTVHGDPHVVRAAKEGAAGSDTKILAVTILTSLDRTDLDDCLIRDGEMLDLVAERAGRAFEAGADGVISSPHEATM
IRALPEAKGRLIVTPGVRPTGSASGDQKRIATPRQAIADGADHIVVGRPIWQHQDPRAAAQMVVADLP

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=236, Percent_Identity=36.4406779661017, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_JANSC (Q28K56)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_511931.1
- ProteinModelPortal:   Q28K56
- SMR:   Q28K56
- STRING:   Q28K56
- GeneID:   3936476
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_3989
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q28K56
- ProtClustDB:   PRK00230
- BioCyc:   JSP290400:JANN_3989-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24215; Mature: 24084

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 63-63 BINDING 11-11 BINDING 33-33 BINDING 116-116 BINDING 179-179 BINDING 188-188 BINDING 208-208 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDRLIVALDVPNVVQGLALAEKLGDAVSFYKIGLGMLTGGGLALANELKQDHGKRIFL
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEE
DMKLFDISATVEAAVRGLAQFNLDFLTVHGDPHVVRAAKEGAAGSDTKILAVTILTSLDR
EEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEHHCCC
TDLDDCLIRDGEMLDLVAERAGRAFEAGADGVISSPHEATMIRALPEAKGRLIVTPGVRP
CCCHHHHHCCCHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCC
TGSASGDQKRIATPRQAIADGADHIVVGRPIWQHQDPRAAAQMVVADLP
CCCCCCCCHHCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SDDRLIVALDVPNVVQGLALAEKLGDAVSFYKIGLGMLTGGGLALANELKQDHGKRIFL
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEE
DMKLFDISATVEAAVRGLAQFNLDFLTVHGDPHVVRAAKEGAAGSDTKILAVTILTSLDR
EEEEEEHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEHHCCC
TDLDDCLIRDGEMLDLVAERAGRAFEAGADGVISSPHEATMIRALPEAKGRLIVTPGVRP
CCCHHHHHCCCHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCC
TGSASGDQKRIATPRQAIADGADHIVVGRPIWQHQDPRAAAQMVVADLP
CCCCCCCCHHCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA