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The map label for this gene is pyrB

Identifier: 89056393

GI number: 89056393

Start: 4001106

End: 4002050

Strand: Direct

Name: pyrB

Synonym: Jann_3902

Alternate gene names: 89056393

Gene position: 4001106-4002050 (Clockwise)

Preceding gene: 89056388

Following gene: 89056394

Centisome position: 92.66

GC content: 61.48

Gene sequence:

>945_bases
ATGACATTCAACCAGCACCATCTCCTTGGCATCGAAGAGCTGTCCCAGACAGAGATCACAACGCTGCTGGATCGCGCCAA
CGTCCATGCCGAGGCCGAGCGCGGCTCGCGCGACCATGGGCATCCGCTGCGGGGTCTCACCCAGATCAACATGTTTTTTG
AGAACTCAACCCGAACCCAGGCGAGTTTCGAGATTGCAGGGAAACGCCTTGGCGCAGATGTGATGAACATGGCGATGCAG
ACCTCTTCGGTGAAAAAGGGCGAAACACTGATCGACACGGCCCTGACCCTCAACGCCATGCACCCCGACCTTCTGGTTGT
GCGCCATCCCCATTCCGGTGCGGTGAAGCTGCTGGCCGACAAGGTGAACTGCGCCGTGTTGAACGCAGGTGACGGGCGGC
ACGAACACCCCACGCAGGCGCTTCTCGACGCGCTTACCATTCGCCGCGCCAAAGGGCGCCTGCATCGGTTGAACGTCGCG
ATCTGCGGCGACATTGCCCATTCCCGTGTGGCGCGGTCGAACATCCTGCTTCTGGGCAAGATGGAAAACCGCATCCGCCT
GATCGGCACGCCTACGCTGATGCCCTCAGGCGTCGCGGATTGGGGGGTGGAAGTCTACGACAACATGGCCGAGGGTCTGG
CGGACTGCGACGTGGTCATGATGCTCCGCCTCCAGAAAGAACGCATGGACGGCGCGTTCATCCCGTCCGAGCGGGAATAT
TACCACCGCTTTGGTCTGGATGCCGCCAAGCTAAGCGTCGCCAAAGACGACGCCATCATCATGCACCCCGGCCCGATGAA
CCGCGGGGTCGAAATCGACGGTCTGCTGGCCGACGACATCAACCGTAGCGTCATTCAGGAACAGGTCGAAATGGGTGTGG
CGGTAAGGATGGCCGTGATGGAGCTTCTGGCCGAAAACCTACGCGACCGGCGGGCGGCGGCGTGA

Upstream 100 bases:

>100_bases
AGATCACCGCGACGATCACGCGACCCTAGGGTTGGGGTCCAAACGCTTGCCGCGTCGCCCACACGCCCCCTATAAGCGGG
CAAACCACGGGGACCGCATC

Downstream 100 bases:

>100_bases
GCGGCGACTACGACTGGACACCACACTTGCAGGAGGGCGAGGAGGTCCTTTGGCAAGGGCGGCCCCATCAACGTTTCAGG
CTCTTTCTGCGCAACGCGGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTFNQHHLLGIEELSQTEITTLLDRANVHAEAERGSRDHGHPLRGLTQINMFFENSTRTQASFEIAGKRLGADVMNMAMQ
TSSVKKGETLIDTALTLNAMHPDLLVVRHPHSGAVKLLADKVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLNVA
ICGDIAHSRVARSNILLLGKMENRIRLIGTPTLMPSGVADWGVEVYDNMAEGLADCDVVMMLRLQKERMDGAFIPSEREY
YHRFGLDAAKLSVAKDDAIIMHPGPMNRGVEIDGLLADDINRSVIQEQVEMGVAVRMAVMELLAENLRDRRAAA

Sequences:

>Translated_314_residues
MTFNQHHLLGIEELSQTEITTLLDRANVHAEAERGSRDHGHPLRGLTQINMFFENSTRTQASFEIAGKRLGADVMNMAMQ
TSSVKKGETLIDTALTLNAMHPDLLVVRHPHSGAVKLLADKVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLNVA
ICGDIAHSRVARSNILLLGKMENRIRLIGTPTLMPSGVADWGVEVYDNMAEGLADCDVVMMLRLQKERMDGAFIPSEREY
YHRFGLDAAKLSVAKDDAIIMHPGPMNRGVEIDGLLADDINRSVIQEQVEMGVAVRMAVMELLAENLRDRRAAA
>Mature_313_residues
TFNQHHLLGIEELSQTEITTLLDRANVHAEAERGSRDHGHPLRGLTQINMFFENSTRTQASFEIAGKRLGADVMNMAMQT
SSVKKGETLIDTALTLNAMHPDLLVVRHPHSGAVKLLADKVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLNVAI
CGDIAHSRVARSNILLLGKMENRIRLIGTPTLMPSGVADWGVEVYDNMAEGLADCDVVMMLRLQKERMDGAFIPSEREYY
HRFGLDAAKLSVAKDDAIIMHPGPMNRGVEIDGLLADDINRSVIQEQVEMGVAVRMAVMELLAENLRDRRAAA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=35.7142857142857, Blast_Score=166, Evalue=2e-41,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=34.5047923322684, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=31.5789473684211, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=34.0764331210191, Blast_Score=138, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=9e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_JANSC (Q28KE3)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_511844.1
- ProteinModelPortal:   Q28KE3
- SMR:   Q28KE3
- STRING:   Q28KE3
- GeneID:   3936383
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_3902
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q28KE3
- ProtClustDB:   PRK00856
- BioCyc:   JSP290400:JANN_3902-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34674; Mature: 34542

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNQHHLLGIEELSQTEITTLLDRANVHAEAERGSRDHGHPLRGLTQINMFFENSTRTQ
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCCH
ASFEIAGKRLGADVMNMAMQTSSVKKGETLIDTALTLNAMHPDLLVVRHPHSGAVKLLAD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCEEEEECCCCCCCCEEEC
KVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLNVAICGDIAHSRVARSNILLLGK
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHCCEEEEEE
MENRIRLIGTPTLMPSGVADWGVEVYDNMAEGLADCDVVMMLRLQKERMDGAFIPSEREY
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
YHRFGLDAAKLSVAKDDAIIMHPGPMNRGVEIDGLLADDINRSVIQEQVEMGVAVRMAVM
HHHHCCCHHHEEEECCCEEEECCCCCCCCEEECCEEHHHCCHHHHHHHHHHHHHHHHHHH
ELLAENLRDRRAAA
HHHHHHHHHHCCCC
>Mature Secondary Structure 
TFNQHHLLGIEELSQTEITTLLDRANVHAEAERGSRDHGHPLRGLTQINMFFENSTRTQ
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEEEEEEECCCCCH
ASFEIAGKRLGADVMNMAMQTSSVKKGETLIDTALTLNAMHPDLLVVRHPHSGAVKLLAD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCEEEEECCCCCCCCEEEC
KVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLNVAICGDIAHSRVARSNILLLGK
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECHHHHHHHHHCCEEEEEE
MENRIRLIGTPTLMPSGVADWGVEVYDNMAEGLADCDVVMMLRLQKERMDGAFIPSEREY
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
YHRFGLDAAKLSVAKDDAIIMHPGPMNRGVEIDGLLADDINRSVIQEQVEMGVAVRMAVM
HHHHCCCHHHEEEECCCEEEECCCCCCCCEEECCEEHHHCCHHHHHHHHHHHHHHHHHHH
ELLAENLRDRRAAA
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA