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The map label for this gene is ghrA [H]

Identifier: 89056141

GI number: 89056141

Start: 3724332

End: 3725273

Strand: Direct

Name: ghrA [H]

Synonym: Jann_3650

Alternate gene names: 89056141

Gene position: 3724332-3725273 (Clockwise)

Preceding gene: 89056140

Following gene: 89056144

Centisome position: 86.25

GC content: 63.91

Gene sequence:

>942_bases
ATGACAGCCCCCACGATCCTATTCGCTGCCAAGCCCGAGCGGTGGATTACGTATGAGCCGCACCTGCGCCACGCGCTGAA
TGCCGTCGGCATTAGCGACGCGGGTCTGACGGTGCAGACCGACCCGGCAGATGTGGATTACATCGTCTACGCGCCCAATT
CCGACGTGCAGGACTTCACGCCCTTCACGCGCCTCAAGGCGGTGCTGAACCTCTGGGCCGGTGTGGAGGATGTGACAGGC
AACGCGACCCTCAAAGCGCCTCTCGCCCGTATGGTCGATCATGGCCTGACCGAAGGCATGGTGGAATGGTGCGTGGGCCA
TACCCTGCGCCACCACCTTGGTATGGATACCCACATCCTCGGGCAGGACGGTGTCTGGCGCGCGGAGTCCATCCCGCCCT
TGGCGCGTAACAGGCCCGTGACAATCCTGGGCCTTGGAGCATTGGGAAAGGCCGTGGCGACGGCGCTGACAGCGCTTGGG
TTTCCGGTCACGGGCTGGTCCCGCTCGGCCAAGGAGATCGCAGGTATGCGTTGTCTGCACGGCGACACCGGCCTGTCTGA
TGCGCTATCGACGGCGCAGATCGTGATCCTGCTGCTGCCCGACACGCCCGCGACCGAGAACATCTTGAACGCCGCAACCC
TGGACGCTTTGCCTACAGGGGCCGTGATCATCAACCCGGGCCGTGGGCCCTTGATCAACGATGGCGCGCTTCTTGCAGCG
CTTGATTCCGGCCAGATTGCTCACGCCACTCTAGATGTCTTTCGCCAGGAACCGCTGCCAGTGGATCACCCGTACTGGGC
GCACCCAAATGTCACCGTCACCCCCCACATCGCATCGGAGACGCGGCCGGATACGGCCTCCGAGGTCATCGCCGAGAACA
TCCGACGCGGTGAGGCCGGAGAGCCCTTTCTCCACCTCGTCGACCGGGCATTGGGTTACTGA

Upstream 100 bases:

>100_bases
GCCATGTTGGTGCTGTTGGCCGCATTCGGTCTTGTCCAATCGGCGCATGTTCATCGGGCCCGGTAGCGGGTCCGCGCGTC
TCCCAAGCAAGGTAGCCCAG

Downstream 100 bases:

>100_bases
TAGCGCGCGGCCTCCCCTTCATCTTGGCCCTACAACTCCGGGGGTCCGGGGCAGGCCCCCGGCGTTGAACTCATCGCAGA
CGCGGCGGTTTGTCAGGATC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTAPTILFAAKPERWITYEPHLRHALNAVGISDAGLTVQTDPADVDYIVYAPNSDVQDFTPFTRLKAVLNLWAGVEDVTG
NATLKAPLARMVDHGLTEGMVEWCVGHTLRHHLGMDTHILGQDGVWRAESIPPLARNRPVTILGLGALGKAVATALTALG
FPVTGWSRSAKEIAGMRCLHGDTGLSDALSTAQIVILLLPDTPATENILNAATLDALPTGAVIINPGRGPLINDGALLAA
LDSGQIAHATLDVFRQEPLPVDHPYWAHPNVTVTPHIASETRPDTASEVIAENIRRGEAGEPFLHLVDRALGY

Sequences:

>Translated_313_residues
MTAPTILFAAKPERWITYEPHLRHALNAVGISDAGLTVQTDPADVDYIVYAPNSDVQDFTPFTRLKAVLNLWAGVEDVTG
NATLKAPLARMVDHGLTEGMVEWCVGHTLRHHLGMDTHILGQDGVWRAESIPPLARNRPVTILGLGALGKAVATALTALG
FPVTGWSRSAKEIAGMRCLHGDTGLSDALSTAQIVILLLPDTPATENILNAATLDALPTGAVIINPGRGPLINDGALLAA
LDSGQIAHATLDVFRQEPLPVDHPYWAHPNVTVTPHIASETRPDTASEVIAENIRRGEAGEPFLHLVDRALGY
>Mature_312_residues
TAPTILFAAKPERWITYEPHLRHALNAVGISDAGLTVQTDPADVDYIVYAPNSDVQDFTPFTRLKAVLNLWAGVEDVTGN
ATLKAPLARMVDHGLTEGMVEWCVGHTLRHHLGMDTHILGQDGVWRAESIPPLARNRPVTILGLGALGKAVATALTALGF
PVTGWSRSAKEIAGMRCLHGDTGLSDALSTAQIVILLLPDTPATENILNAATLDALPTGAVIINPGRGPLINDGALLAAL
DSGQIAHATLDVFRQEPLPVDHPYWAHPNVTVTPHIASETRPDTASEVIAENIRRGEAGEPFLHLVDRALGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=169, Percent_Identity=35.5029585798817, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI23308577, Length=166, Percent_Identity=32.5301204819277, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4557497, Length=262, Percent_Identity=26.3358778625954, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI61743967, Length=262, Percent_Identity=26.3358778625954, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI145580578, Length=261, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557499, Length=261, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081824, Length=257, Percent_Identity=40.0778210116732, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI87082289, Length=146, Percent_Identity=36.3013698630137, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789279, Length=215, Percent_Identity=28.3720930232558, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=185, Percent_Identity=32.4324324324324, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6325144, Length=148, Percent_Identity=33.1081081081081, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=176, Percent_Identity=31.8181818181818, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=165, Percent_Identity=30.9090909090909, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322116, Length=164, Percent_Identity=28.0487804878049, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320925, Length=164, Percent_Identity=28.0487804878049, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574286, Length=176, Percent_Identity=32.9545454545455, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574284, Length=268, Percent_Identity=29.4776119402985, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28571528, Length=175, Percent_Identity=31.4285714285714, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI45552429, Length=268, Percent_Identity=29.4776119402985, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45551003, Length=268, Percent_Identity=29.4776119402985, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585514, Length=268, Percent_Identity=29.1044776119403, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574282, Length=268, Percent_Identity=29.1044776119403, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921140, Length=146, Percent_Identity=30.1369863013699, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585516, Length=204, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33417; Mature: 33286

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPTILFAAKPERWITYEPHLRHALNAVGISDAGLTVQTDPADVDYIVYAPNSDVQDFT
CCCCEEEEEECCCCEEEECHHHHHHHHHCCCCCCCCEEECCCCCCCEEEECCCCCCCCCC
PFTRLKAVLNLWAGVEDVTGNATLKAPLARMVDHGLTEGMVEWCVGHTLRHHLGMDTHIL
HHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEE
GQDGVWRAESIPPLARNRPVTILGLGALGKAVATALTALGFPVTGWSRSAKEIAGMRCLH
CCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCEEEEC
GDTGLSDALSTAQIVILLLPDTPATENILNAATLDALPTGAVIINPGRGPLINDGALLAA
CCCCHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCCEEEEE
LDSGQIAHATLDVFRQEPLPVDHPYWAHPNVTVTPHIASETRPDTASEVIAENIRRGEAG
ECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCC
EPFLHLVDRALGY
CHHHHHHHHHHCC
>Mature Secondary Structure 
TAPTILFAAKPERWITYEPHLRHALNAVGISDAGLTVQTDPADVDYIVYAPNSDVQDFT
CCCEEEEEECCCCEEEECHHHHHHHHHCCCCCCCCEEECCCCCCCEEEECCCCCCCCCC
PFTRLKAVLNLWAGVEDVTGNATLKAPLARMVDHGLTEGMVEWCVGHTLRHHLGMDTHIL
HHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEE
GQDGVWRAESIPPLARNRPVTILGLGALGKAVATALTALGFPVTGWSRSAKEIAGMRCLH
CCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCEEEEC
GDTGLSDALSTAQIVILLLPDTPATENILNAATLDALPTGAVIINPGRGPLINDGALLAA
CCCCHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEECCCEEEEE
LDSGQIAHATLDVFRQEPLPVDHPYWAHPNVTVTPHIASETRPDTASEVIAENIRRGEAG
ECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCC
EPFLHLVDRALGY
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]