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The map label for this gene is leuA [H]

Identifier: 89056134

GI number: 89056134

Start: 3716366

End: 3717925

Strand: Direct

Name: leuA [H]

Synonym: Jann_3643

Alternate gene names: 89056134

Gene position: 3716366-3717925 (Clockwise)

Preceding gene: 89056129

Following gene: 89056135

Centisome position: 86.07

GC content: 62.18

Gene sequence:

>1560_bases
ATGACCTCTGAAAACAGAGTCCTGATTTTTGACACCACCCTGCGCGATGGCGAGCAATCGCCGGGCGCAACCATGAGCCA
TGATGAGAAGTTGGAGATTGCCACGCTTCTGGATGAGATGGGCGTCGACATCATCGAGGCGGGCTTCCCGATTGCCAGCG
AGGGCGATTTCAACGCCGTGTCCGAGATCGCGAAAAACGCCACAAAGTCCACGATCTGCGGGCTGGCGCGCGCCAACTTC
AAGGACATCGACCGAGCGTGGGAGGCGGTGAAACACGCCAAATCGCCGCGCATCCACACGTTTATCGGGACCTCTCCGCA
ACACCGCGCGATCCCGAACCTGACCATGGACGAGATGGCCGACCGCATTCACGAAACGGTCACTCACGCCCGCAACCTCT
GTGACAATGTGCAATGGTCGCCGATGGACGCCACGAGGACGGGGTGGGACTATCTGGCCCGCACCGTGGAGATCGCGATC
AAGGCGGGCGCCACGACAATCAACATCCCCGATACCTGCGGCTATACGGCGCCGGTGGAAAGCGCAGATCTGATCCGTCG
TCTGATCGCGGAGGTGCCGGGCGCGGACGAGATTATCTTCGCCACGCATTGCCACAACGATCTGGGCATGGCGACTGCGA
ACTCCCTGGCGGCGGTCGACGGCGGCGCGCGGCAAATTGAGTGTACCATAAACGGTCTGGGGGAACGCGCGGGCAACACC
GCCTTGGAAGAGGTCGTGATGGCCATGAAGGTCCGCCACGACATCATGCCCTACACCTCCGGGATCGACACCACCAAGAT
CATGCACCTGTCTCGCCGGGTGGCGACGGTGTCCGGCTTCAACGTGCAGCCCAACAAGGCCATCGTCGGCAAGAACGCCT
TTGCCCATGAGTCTGGCATCCATCAGGACGGGATGCTGAAATCCGCCGATACGTTTGAAATCATGCGCCCGGAGGACGTC
GGGCTGAGTGAGACGAATATTGTCATGGGCAAGCACTCCGGTCGCGCGGCGTTGCGGTCCAAGTTGAAAGACCTGGGCTA
TGACATGGCCGACAACCAGCTGAACGACCTGTTCGTGCGGTTCAAGGCGTTGGCGGACCGGAAGAAAGAGGTCTTCGACG
ATGACCTGATCGCGCTGGTCACCGATGCCGGGAGCGATGGTGAAGGTCATCTGGAGGTGAAATTCCTGCGTGTGATATGC
GGGACGGAGGCGCCGCAAAGCGCCGACCTGACCCTGCGCGTGGGCGACACCGATCACCAGGCCACGGCCCAGGGCGACGG
CCCCGTGGACGCGGCCTTCAATGCGGTCAAAGCGGTCTTCCCCAATGAGGCGGCGTTACAACTCTATCAGGTGAGCGCCG
TGACCGAGGGCATGGACGCCCAGGCCACCGTGACCGTGCGGGTCGAAGAAGGCGGGCGCATCGCCACGGGCCAGTCGGCG
GATACCGATACGGTCGTGGCCAGCGTGAAAGCGTATGTGAACGCCCTCAACCGCCTGATCGTACGGCGAGAGAAGACGGG
CGGCGACGTGCGAGAGGTCAGCTACAAGGACGTAGGGTAG

Upstream 100 bases:

>100_bases
TCTCCTCCTCCTTAGCCGCCTCTGAGCGTGCCTTTCGGCGCGACCGCTCAGGGGAGACACCGCGCCGGAGACACCTCAAT
CTACCGCTAAGGAACACGAC

Downstream 100 bases:

>100_bases
TGCGCGGGGCGTCTTTTGTCGCCACGGGCTTCTGCGTCGGATTAGGGGGAATGGCCGTGCATGCGGCCTGCCCCACCGTC
GCCGATCTGGATGGTGGGAT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTSENRVLIFDTTLRDGEQSPGATMSHDEKLEIATLLDEMGVDIIEAGFPIASEGDFNAVSEIAKNATKSTICGLARANF
KDIDRAWEAVKHAKSPRIHTFIGTSPQHRAIPNLTMDEMADRIHETVTHARNLCDNVQWSPMDATRTGWDYLARTVEIAI
KAGATTINIPDTCGYTAPVESADLIRRLIAEVPGADEIIFATHCHNDLGMATANSLAAVDGGARQIECTINGLGERAGNT
ALEEVVMAMKVRHDIMPYTSGIDTTKIMHLSRRVATVSGFNVQPNKAIVGKNAFAHESGIHQDGMLKSADTFEIMRPEDV
GLSETNIVMGKHSGRAALRSKLKDLGYDMADNQLNDLFVRFKALADRKKEVFDDDLIALVTDAGSDGEGHLEVKFLRVIC
GTEAPQSADLTLRVGDTDHQATAQGDGPVDAAFNAVKAVFPNEAALQLYQVSAVTEGMDAQATVTVRVEEGGRIATGQSA
DTDTVVASVKAYVNALNRLIVRREKTGGDVREVSYKDVG

Sequences:

>Translated_519_residues
MTSENRVLIFDTTLRDGEQSPGATMSHDEKLEIATLLDEMGVDIIEAGFPIASEGDFNAVSEIAKNATKSTICGLARANF
KDIDRAWEAVKHAKSPRIHTFIGTSPQHRAIPNLTMDEMADRIHETVTHARNLCDNVQWSPMDATRTGWDYLARTVEIAI
KAGATTINIPDTCGYTAPVESADLIRRLIAEVPGADEIIFATHCHNDLGMATANSLAAVDGGARQIECTINGLGERAGNT
ALEEVVMAMKVRHDIMPYTSGIDTTKIMHLSRRVATVSGFNVQPNKAIVGKNAFAHESGIHQDGMLKSADTFEIMRPEDV
GLSETNIVMGKHSGRAALRSKLKDLGYDMADNQLNDLFVRFKALADRKKEVFDDDLIALVTDAGSDGEGHLEVKFLRVIC
GTEAPQSADLTLRVGDTDHQATAQGDGPVDAAFNAVKAVFPNEAALQLYQVSAVTEGMDAQATVTVRVEEGGRIATGQSA
DTDTVVASVKAYVNALNRLIVRREKTGGDVREVSYKDVG
>Mature_518_residues
TSENRVLIFDTTLRDGEQSPGATMSHDEKLEIATLLDEMGVDIIEAGFPIASEGDFNAVSEIAKNATKSTICGLARANFK
DIDRAWEAVKHAKSPRIHTFIGTSPQHRAIPNLTMDEMADRIHETVTHARNLCDNVQWSPMDATRTGWDYLARTVEIAIK
AGATTINIPDTCGYTAPVESADLIRRLIAEVPGADEIIFATHCHNDLGMATANSLAAVDGGARQIECTINGLGERAGNTA
LEEVVMAMKVRHDIMPYTSGIDTTKIMHLSRRVATVSGFNVQPNKAIVGKNAFAHESGIHQDGMLKSADTFEIMRPEDVG
LSETNIVMGKHSGRAALRSKLKDLGYDMADNQLNDLFVRFKALADRKKEVFDDDLIALVTDAGSDGEGHLEVKFLRVICG
TEAPQSADLTLRVGDTDHQATAQGDGPVDAAFNAVKAVFPNEAALQLYQVSAVTEGMDAQATVTVRVEEGGRIATGQSAD
TDTVVASVKAYVNALNRLIVRREKTGGDVREVSYKDVG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=496, Percent_Identity=45.3629032258064, Blast_Score=410, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6320019, Length=377, Percent_Identity=29.973474801061, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320071, Length=369, Percent_Identity=30.6233062330623, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=541, Percent_Identity=24.9537892791128, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324225, Length=539, Percent_Identity=25.0463821892393, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56035; Mature: 55903

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSENRVLIFDTTLRDGEQSPGATMSHDEKLEIATLLDEMGVDIIEAGFPIASEGDFNAV
CCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCHHHH
SEIAKNATKSTICGLARANFKDIDRAWEAVKHAKSPRIHTFIGTSPQHRAIPNLTMDEMA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHH
DRIHETVTHARNLCDNVQWSPMDATRTGWDYLARTVEIAIKAGATTINIPDTCGYTAPVE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEEEECCCEEEECCCCCCCCCCCC
SADLIRRLIAEVPGADEIIFATHCHNDLGMATANSLAAVDGGARQIECTINGLGERAGNT
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCCHHEECCCCEEEEEEEECCCCCCCCH
ALEEVVMAMKVRHDIMPYTSGIDTTKIMHLSRRVATVSGFNVQPNKAIVGKNAFAHESGI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEECCCCHHHCCCC
HQDGMLKSADTFEIMRPEDVGLSETNIVMGKHSGRAALRSKLKDLGYDMADNQLNDLFVR
CCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
FKALADRKKEVFDDDLIALVTDAGSDGEGHLEVKFLRVICGTEAPQSADLTLRVGDTDHQ
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCC
ATAQGDGPVDAAFNAVKAVFPNEAALQLYQVSAVTEGMDAQATVTVRVEEGGRIATGQSA
CCCCCCCCHHHHHHHHHHHCCCHHHHHEEEHHHHHCCCCCEEEEEEEECCCCEEECCCCC
DTDTVVASVKAYVNALNRLIVRREKTGGDVREVSYKDVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
>Mature Secondary Structure 
TSENRVLIFDTTLRDGEQSPGATMSHDEKLEIATLLDEMGVDIIEAGFPIASEGDFNAV
CCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCCCCHHHH
SEIAKNATKSTICGLARANFKDIDRAWEAVKHAKSPRIHTFIGTSPQHRAIPNLTMDEMA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHH
DRIHETVTHARNLCDNVQWSPMDATRTGWDYLARTVEIAIKAGATTINIPDTCGYTAPVE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEEEECCCEEEECCCCCCCCCCCC
SADLIRRLIAEVPGADEIIFATHCHNDLGMATANSLAAVDGGARQIECTINGLGERAGNT
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHCCCHHEECCCCEEEEEEEECCCCCCCCH
ALEEVVMAMKVRHDIMPYTSGIDTTKIMHLSRRVATVSGFNVQPNKAIVGKNAFAHESGI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCEEECCCCHHHCCCC
HQDGMLKSADTFEIMRPEDVGLSETNIVMGKHSGRAALRSKLKDLGYDMADNQLNDLFVR
CCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
FKALADRKKEVFDDDLIALVTDAGSDGEGHLEVKFLRVICGTEAPQSADLTLRVGDTDHQ
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCCC
ATAQGDGPVDAAFNAVKAVFPNEAALQLYQVSAVTEGMDAQATVTVRVEEGGRIATGQSA
CCCCCCCCHHHHHHHHHHHCCCHHHHHEEEHHHHHCCCCCEEEEEEEECCCCEEECCCCC
DTDTVVASVKAYVNALNRLIVRREKTGGDVREVSYKDVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA