Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 89056075

GI number: 89056075

Start: 3663309

End: 3664100

Strand: Direct

Name: trpA

Synonym: Jann_3584

Alternate gene names: 89056075

Gene position: 3663309-3664100 (Clockwise)

Preceding gene: 89056074

Following gene: 89056076

Centisome position: 84.84

GC content: 65.28

Gene sequence:

>792_bases
ATGACCCGTATCGACGACACTTTCGCGCGCCTGTCCGCATCCGGCAAAAAGGCCTTCGTCTCCTACATCATGGCGGGCGA
TCCGGATTACGCGACCTCGCTTGAGGTCATGCGGGGTCTGCCCGCTGCCGGCGTCGACATCATCGAATTGGGGCTGCCAT
TCACGGACCCGATGGCCGACGGCTCCACCATCCAGCTGGCCGGGCAACGCGCCCTCGACGGTGGCATGACACTGGACAAG
ACGTTGCAGATGGTCCGCGATTTTCGCGCCGGGGACGACACGACGCCGATTGTGCTGATGGGCTATTACAACCCGATCTA
TTCGCGCGGCGTGGACCGGTTCCTGACCGACGCAAGGGAAGCGGGCATTGACGGGCTGATCGTCGTCGACCTGCCGCCCG
AGGAAGATGAAGAGCTGTGCATTCCCGCCCAGGCCGCTGGTCTGAACTTCATCCGGCTGGCCACGCCAACCACCGACGAC
GCCCGCCTCCCGGCCGTGCTCAGCAACACGTCGGGGTTTCTCTACTATGTCTCCATCACCGGCATCACTGGGGCCGCCGC
GCCCCAGGCCGCTGACGTGGCCCCCGAAGTCGCTCGCATCAAATCCGCCACCGACATACCGGTGATCGTGGGCTTCGGCA
TCACCACATCCGAGGCCGCCCGGACCATCGCAGGTGTGGCCGATGGCTGCGTCGTCGGATCAGCGATCGTGTCCAGGATC
GCAGAGGGCGAGCCCGTGGCCGATGTTCTGGCCTTCGTCAAAAGCCTCTCGGACGGGGCGCACGCGGCCTGA

Upstream 100 bases:

>100_bases
CAGAGCTTTCGGGAAAAACCCACGCCCTTTCCCCCCATTGATTTCCCTCTGCCCTTGCGCCAAACCAGCGCCCACATCGC
CCAGTTGAGAGCCTGCATCC

Downstream 100 bases:

>100_bases
CGGACGGCGGCGCGCTGCGGCACGCGGTCTCAGGGCCGTTTTCCGGTCAATTCCATCCTGGACCGCTCTGCGAATCGCTA
TCGCCTCGCCCGGCGCATTG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTRIDDTFARLSASGKKAFVSYIMAGDPDYATSLEVMRGLPAAGVDIIELGLPFTDPMADGSTIQLAGQRALDGGMTLDK
TLQMVRDFRAGDDTTPIVLMGYYNPIYSRGVDRFLTDAREAGIDGLIVVDLPPEEDEELCIPAQAAGLNFIRLATPTTDD
ARLPAVLSNTSGFLYYVSITGITGAAAPQAADVAPEVARIKSATDIPVIVGFGITTSEAARTIAGVADGCVVGSAIVSRI
AEGEPVADVLAFVKSLSDGAHAA

Sequences:

>Translated_263_residues
MTRIDDTFARLSASGKKAFVSYIMAGDPDYATSLEVMRGLPAAGVDIIELGLPFTDPMADGSTIQLAGQRALDGGMTLDK
TLQMVRDFRAGDDTTPIVLMGYYNPIYSRGVDRFLTDAREAGIDGLIVVDLPPEEDEELCIPAQAAGLNFIRLATPTTDD
ARLPAVLSNTSGFLYYVSITGITGAAAPQAADVAPEVARIKSATDIPVIVGFGITTSEAARTIAGVADGCVVGSAIVSRI
AEGEPVADVLAFVKSLSDGAHAA
>Mature_262_residues
TRIDDTFARLSASGKKAFVSYIMAGDPDYATSLEVMRGLPAAGVDIIELGLPFTDPMADGSTIQLAGQRALDGGMTLDKT
LQMVRDFRAGDDTTPIVLMGYYNPIYSRGVDRFLTDAREAGIDGLIVVDLPPEEDEELCIPAQAAGLNFIRLATPTTDDA
RLPAVLSNTSGFLYYVSITGITGAAAPQAADVAPEVARIKSATDIPVIVGFGITTSEAARTIAGVADGCVVGSAIVSRIA
EGEPVADVLAFVKSLSDGAHAA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=263, Percent_Identity=33.0798479087452, Blast_Score=135, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=262, Percent_Identity=40.8396946564886, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_JANSC (Q28LB1)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_511526.1
- HSSP:   P00929
- ProteinModelPortal:   Q28LB1
- SMR:   Q28LB1
- STRING:   Q28LB1
- GeneID:   3936059
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_3584
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q28LB1
- ProtClustDB:   PRK13111
- BioCyc:   JSP290400:JANN_3584-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27373; Mature: 27242

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIDDTFARLSASGKKAFVSYIMAGDPDYATSLEVMRGLPAAGVDIIELGLPFTDPMAD
CCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GSTIQLAGQRALDGGMTLDKTLQMVRDFRAGDDTTPIVLMGYYNPIYSRGVDRFLTDARE
CCEEEEECCHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGIDGLIVVDLPPEEDEELCIPAQAAGLNFIRLATPTTDDARLPAVLSNTSGFLYYVSIT
CCCCCEEEEECCCCCCCCEEEEHHHCCCEEEEEECCCCCCCCCCCEEECCCCEEEEEEEE
GITGAAAPQAADVAPEVARIKSATDIPVIVGFGITTSEAARTIAGVADGCVVGSAIVSRI
CCCCCCCCCHHHCCHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEGEPVADVLAFVKSLSDGAHAA
HCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRIDDTFARLSASGKKAFVSYIMAGDPDYATSLEVMRGLPAAGVDIIELGLPFTDPMAD
CCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GSTIQLAGQRALDGGMTLDKTLQMVRDFRAGDDTTPIVLMGYYNPIYSRGVDRFLTDARE
CCEEEEECCHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGIDGLIVVDLPPEEDEELCIPAQAAGLNFIRLATPTTDDARLPAVLSNTSGFLYYVSIT
CCCCCEEEEECCCCCCCCEEEEHHHCCCEEEEEECCCCCCCCCCCEEECCCCEEEEEEEE
GITGAAAPQAADVAPEVARIKSATDIPVIVGFGITTSEAARTIAGVADGCVVGSAIVSRI
CCCCCCCCCHHHCCHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEGEPVADVLAFVKSLSDGAHAA
HCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA