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The map label for this gene is ogt [H]

Identifier: 89055961

GI number: 89055961

Start: 3522994

End: 3523449

Strand: Direct

Name: ogt [H]

Synonym: Jann_3470

Alternate gene names: 89055961

Gene position: 3522994-3523449 (Clockwise)

Preceding gene: 89055960

Following gene: 89055963

Centisome position: 81.59

GC content: 63.82

Gene sequence:

>456_bases
ATGAGCAGGATATCCGTTGAAACACCAATCGGCCCGGTCTGGGTGGAAGCGCAGGACGGGGCGATCATCGGCGCTGGATG
GGGGGCCGTTGAGAGCCCCGGCGACAGCGATCTTCTGCGTCAGACAGCGGCCGAATTGCGGGGTTACTTCGACGGCACAT
TGCAGCGCTTCACGGTGCCAATCCGCCATGGCCGCACGAACGCGACGGGCAAAGTCCTGGACGCGATGCTGGCCATCCCG
ATGGGCGAGACGCGGGAATATGGCGACATCGCGCGGGAGGTCGGGTTGCCCGCCCAAGCGGTCGGTCAGGCCTGCGGTGG
CAACTGCATCCCAATCCTGATCCCCTGCCACCGGGTCTTGTCAGCCACTGGCCTCGGCGGGTTTTCCGCCCCTGGAGGTG
TGGATACGAAAGTATGGCTTTTGCGACATGAAGGGGCGGCTGGCTTGCTTATCTAA

Upstream 100 bases:

>100_bases
CAACGGGGGACGTTGGTTGATCCGCTCACCGCGCGCGGGCCGATCCGTCTTGCGCAGGATCCGGGTTGATCGGAGAGGCC
TCGCGGCGCAGGGTTCGGGC

Downstream 100 bases:

>100_bases
GCCGTGACCTTGCCTTATCCTGACAACAACCAAAGGTTATCCACAGGCTATTTCTTCCATGTCACGCGGGCCTCTGCGGC
TGTCATGGGCCCTGTCGCGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MSRISVETPIGPVWVEAQDGAIIGAGWGAVESPGDSDLLRQTAAELRGYFDGTLQRFTVPIRHGRTNATGKVLDAMLAIP
MGETREYGDIAREVGLPAQAVGQACGGNCIPILIPCHRVLSATGLGGFSAPGGVDTKVWLLRHEGAAGLLI

Sequences:

>Translated_151_residues
MSRISVETPIGPVWVEAQDGAIIGAGWGAVESPGDSDLLRQTAAELRGYFDGTLQRFTVPIRHGRTNATGKVLDAMLAIP
MGETREYGDIAREVGLPAQAVGQACGGNCIPILIPCHRVLSATGLGGFSAPGGVDTKVWLLRHEGAAGLLI
>Mature_150_residues
SRISVETPIGPVWVEAQDGAIIGAGWGAVESPGDSDLLRQTAAELRGYFDGTLQRFTVPIRHGRTNATGKVLDAMLAIPM
GETREYGDIAREVGLPAQAVGQACGGNCIPILIPCHRVLSATGLGGFSAPGGVDTKVWLLRHEGAAGLLI

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=169, Percent_Identity=34.9112426035503, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787596, Length=166, Percent_Identity=34.3373493975904, Blast_Score=75, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=79, Percent_Identity=46.8354430379747, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=89, Percent_Identity=43.8202247191011, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 15731; Mature: 15600

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISVETPIGPVWVEAQDGAIIGAGWGAVESPGDSDLLRQTAAELRGYFDGTLQRFTVP
CCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEE
IRHGRTNATGKVLDAMLAIPMGETREYGDIAREVGLPAQAVGQACGGNCIPILIPCHRVL
ECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHH
SATGLGGFSAPGGVDTKVWLLRHEGAAGLLI
HHCCCCCCCCCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
SRISVETPIGPVWVEAQDGAIIGAGWGAVESPGDSDLLRQTAAELRGYFDGTLQRFTVP
CCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEE
IRHGRTNATGKVLDAMLAIPMGETREYGDIAREVGLPAQAVGQACGGNCIPILIPCHRVL
ECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEHHHHHH
SATGLGGFSAPGGVDTKVWLLRHEGAAGLLI
HHCCCCCCCCCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]