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The map label for this gene is dnaG

Identifier: 89055940

GI number: 89055940

Start: 3495842

End: 3497782

Strand: Direct

Name: dnaG

Synonym: Jann_3449

Alternate gene names: 89055940

Gene position: 3495842-3497782 (Clockwise)

Preceding gene: 89055939

Following gene: 89055941

Centisome position: 80.96

GC content: 65.74

Gene sequence:

>1941_bases
ATGTCATTGCCCCCCGGATTCCTTGACGAGCTGCGCACCCGCACCTCCATCGCGCAAGTGGTGGGGCGCAAGGTGATCTG
GGACAATCGCAAATCCAATCAGGCCAAGGGCGACATGTGGGCGCCCTGCCCGTTCCATCAGGAAAAAAGCGCCAGCTTCC
ACGTCGATGACCGCAAGGGGTTTTACTATTGCTTCGGCTGTCAGGCGAAGGGCGACATGTTCAAATTCGTCCAGGAGACG
GAGAATGTGGGCTTCATGGAAGCGGTGCAGATCCTCGCCGGTGAAGCCGGCATGCAGATGCCCGCGCGCGATCCACAGGC
GCAGGAAAAGGCCGACAAGGCCACGCAACTGGCCAAGGTGACGGAGGCTGCGGTCCAGCACTTTCGCCTGCAACTCAAGA
CCCAGGCCGCGTCGGACGCCCGCGCCTATCTGGAAGGGCGCGGCATGGATCAGGCCACGCTGGACCGGTTCGAGATCGGC
TTTGCCCCCAATGGCCGCAACAGCACCCATCAGGCGCTGGTCGATAAGGGCTTTGCCGTTGACGACATCACGGCGGCCAA
TGTCTCCACCAAGCCCGACGACGGCGGCGCGCCCTATGATGCGTTTCGCGACCGGATCATCTTTCCCATCCGCGACGCGC
GCGGGCGGTGTCTGGGCTTTGGCGGGCGGGCGATGGACCCCAATGCGCGCGCCAAGTATCTTAATTCGCGGGAAACGCCG
CTCTTTGACAAGGGCCGCGCGCTCTACAATCACGGCCCCGCGCGGGAGGCAGCAGGCAAGGGCCAGCCCCTGATCGTGGC
CGAGGGCTACATGGATGTCATCGCGCTGGTGGGTGCGGGGTTTGAGGCCGCAGTGGCCCCCCTCGGCACCGCGGTCACGG
AAGATCAGCTGCGCCTGATGTGGCGCATCACCGACGAGCCGATCATCGCGCTGGACGGGGACAAGGCGGGTCTGCGCGCC
GCGATGCGGCTGGTGGATCTGGCAATGCCGCTGCTGGAGGGCGGAAAGAGCCTGCGCTTTGTGATCCTGCCCGAGGGCCA
GGACCCCGATGATCTGATCCGCGCCCAGGGGCCGGGCGCGATGCAGGCGTTGCTGGACAAGGCAGAGCCGATGGTCACGC
TGCTCTGGCGGCGAGAGACGGAAGGCCAGGTGTTCGACAGCCCCGAGCGGCGGTCCGCCCTTGATGGGCGATTGCGGCAG
GCGCTGCAACGGATCGGCGATCCGGGCCTGCGCCGCCATTACGGCGATGCGATTTCCGAGCTGCGGCGCGCCCTGTTCCG
ACCGCAGCAGAAACCGTGGCAGCCCGGGAAAAGAGGCCCGTGGAAAGGCCGTGGCTTCGCGGAGGCGCAGGCGCCCCTGG
CCGCCACGCGCGCCCGCGTCGGTGCGCTGAGCGGGGAGACCCTGCGCACCGGGTTGATCGTGGCCACCTGCATCCGGTTC
CCCGATCTGATTGATGTGTTCGAGCCGCAGCTTGACCGCATGAACCCCCGCGATCCCGATCACGCGCGCCTCACCGCCCA
TCTGTTGCGGACCCAAAACCGGGAGGCCGCGACCCTGTTGGACGAAATAAATGCTTCCCCCCTCGCGCCCGCCCTTGAAA
GCCTCTTGCGGCTTTCCCATCTCGCGATCACGCCGTTCTTGATCGGCGCGGGAGATCGCGCGCGCGCGGAAGATTGCCTG
GAAGAGGAATTTGCGCGGCTCACCGCTGCACGGGCCATTGAGGATGAGATGGTGGAGGCCAGCCAGGACGTGGAAGCCGC
GCCAGATGAAGGGGACCCGGCGCTGGCCTACCGCATGAAAGAGGCTTTGCGCGGCAAGCACGCGGCCCAAAGTCCCAAAG
GCGAGGATAACGCTGAACAATTCGGGGAAACGTCCGAGGCACGCTCGGCCTTCCACCAGTTGCTCTCGACGGCCGGACAG
GGCAAGAAATTAAGGAAATAG

Upstream 100 bases:

>100_bases
TTGCGGCGCAAGGACAAACTCGTTGGCCCGCCGATGAGGGGCGCGAATTCACCTCGGCCCCACCCTAGACACGGCCCCGC
CGCCGCCCGATATTGCGTCC

Downstream 100 bases:

>100_bases
ACCTGTCCGAATCAGTGATTCGCACTAGGATATGGTTTCAGACACAATGATTCGCCCGTCACGCCGGTGCGGATGGACTT
AGTCCGAAAGGGTAGACGCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MSLPPGFLDELRTRTSIAQVVGRKVIWDNRKSNQAKGDMWAPCPFHQEKSASFHVDDRKGFYYCFGCQAKGDMFKFVQET
ENVGFMEAVQILAGEAGMQMPARDPQAQEKADKATQLAKVTEAAVQHFRLQLKTQAASDARAYLEGRGMDQATLDRFEIG
FAPNGRNSTHQALVDKGFAVDDITAANVSTKPDDGGAPYDAFRDRIIFPIRDARGRCLGFGGRAMDPNARAKYLNSRETP
LFDKGRALYNHGPAREAAGKGQPLIVAEGYMDVIALVGAGFEAAVAPLGTAVTEDQLRLMWRITDEPIIALDGDKAGLRA
AMRLVDLAMPLLEGGKSLRFVILPEGQDPDDLIRAQGPGAMQALLDKAEPMVTLLWRRETEGQVFDSPERRSALDGRLRQ
ALQRIGDPGLRRHYGDAISELRRALFRPQQKPWQPGKRGPWKGRGFAEAQAPLAATRARVGALSGETLRTGLIVATCIRF
PDLIDVFEPQLDRMNPRDPDHARLTAHLLRTQNREAATLLDEINASPLAPALESLLRLSHLAITPFLIGAGDRARAEDCL
EEEFARLTAARAIEDEMVEASQDVEAAPDEGDPALAYRMKEALRGKHAAQSPKGEDNAEQFGETSEARSAFHQLLSTAGQ
GKKLRK

Sequences:

>Translated_646_residues
MSLPPGFLDELRTRTSIAQVVGRKVIWDNRKSNQAKGDMWAPCPFHQEKSASFHVDDRKGFYYCFGCQAKGDMFKFVQET
ENVGFMEAVQILAGEAGMQMPARDPQAQEKADKATQLAKVTEAAVQHFRLQLKTQAASDARAYLEGRGMDQATLDRFEIG
FAPNGRNSTHQALVDKGFAVDDITAANVSTKPDDGGAPYDAFRDRIIFPIRDARGRCLGFGGRAMDPNARAKYLNSRETP
LFDKGRALYNHGPAREAAGKGQPLIVAEGYMDVIALVGAGFEAAVAPLGTAVTEDQLRLMWRITDEPIIALDGDKAGLRA
AMRLVDLAMPLLEGGKSLRFVILPEGQDPDDLIRAQGPGAMQALLDKAEPMVTLLWRRETEGQVFDSPERRSALDGRLRQ
ALQRIGDPGLRRHYGDAISELRRALFRPQQKPWQPGKRGPWKGRGFAEAQAPLAATRARVGALSGETLRTGLIVATCIRF
PDLIDVFEPQLDRMNPRDPDHARLTAHLLRTQNREAATLLDEINASPLAPALESLLRLSHLAITPFLIGAGDRARAEDCL
EEEFARLTAARAIEDEMVEASQDVEAAPDEGDPALAYRMKEALRGKHAAQSPKGEDNAEQFGETSEARSAFHQLLSTAGQ
GKKLRK
>Mature_645_residues
SLPPGFLDELRTRTSIAQVVGRKVIWDNRKSNQAKGDMWAPCPFHQEKSASFHVDDRKGFYYCFGCQAKGDMFKFVQETE
NVGFMEAVQILAGEAGMQMPARDPQAQEKADKATQLAKVTEAAVQHFRLQLKTQAASDARAYLEGRGMDQATLDRFEIGF
APNGRNSTHQALVDKGFAVDDITAANVSTKPDDGGAPYDAFRDRIIFPIRDARGRCLGFGGRAMDPNARAKYLNSRETPL
FDKGRALYNHGPAREAAGKGQPLIVAEGYMDVIALVGAGFEAAVAPLGTAVTEDQLRLMWRITDEPIIALDGDKAGLRAA
MRLVDLAMPLLEGGKSLRFVILPEGQDPDDLIRAQGPGAMQALLDKAEPMVTLLWRRETEGQVFDSPERRSALDGRLRQA
LQRIGDPGLRRHYGDAISELRRALFRPQQKPWQPGKRGPWKGRGFAEAQAPLAATRARVGALSGETLRTGLIVATCIRFP
DLIDVFEPQLDRMNPRDPDHARLTAHLLRTQNREAATLLDEINASPLAPALESLLRLSHLAITPFLIGAGDRARAEDCLE
EEFARLTAARAIEDEMVEASQDVEAAPDEGDPALAYRMKEALRGKHAAQSPKGEDNAEQFGETSEARSAFHQLLSTAGQG
KKLRK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=405, Percent_Identity=36.7901234567901, Blast_Score=265, Evalue=5e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70949; Mature: 70818

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPPGFLDELRTRTSIAQVVGRKVIWDNRKSNQAKGDMWAPCPFHQEKSASFHVDDRKG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
FYYCFGCQAKGDMFKFVQETENVGFMEAVQILAGEAGMQMPARDPQAQEKADKATQLAKV
EEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEAAVQHFRLQLKTQAASDARAYLEGRGMDQATLDRFEIGFAPNGRNSTHQALVDKGFAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCCCCHHHHHHHCCCCC
DDITAANVSTKPDDGGAPYDAFRDRIIFPIRDARGRCLGFGGRAMDPNARAKYLNSRETP
CCCEECCCCCCCCCCCCCHHHHHCCEEEEEECCCCCEEECCCCCCCCCHHHHHCCCCCCC
LFDKGRALYNHGPAREAAGKGQPLIVAEGYMDVIALVGAGFEAAVAPLGTAVTEDQLRLM
CCCCCCHHHCCCCCHHHCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHEE
WRITDEPIIALDGDKAGLRAAMRLVDLAMPLLEGGKSLRFVILPEGQDPDDLIRAQGPGA
EEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCHH
MQALLDKAEPMVTLLWRRETEGQVFDSPERRSALDGRLRQALQRIGDPGLRRHYGDAISE
HHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRRALFRPQQKPWQPGKRGPWKGRGFAEAQAPLAATRARVGALSGETLRTGLIVATCIRF
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
PDLIDVFEPQLDRMNPRDPDHARLTAHLLRTQNREAATLLDEINASPLAPALESLLRLSH
HHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
LAITPFLIGAGDRARAEDCLEEEFARLTAARAIEDEMVEASQDVEAAPDEGDPALAYRMK
HHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EALRGKHAAQSPKGEDNAEQFGETSEARSAFHQLLSTAGQGKKLRK
HHHCCCHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLPPGFLDELRTRTSIAQVVGRKVIWDNRKSNQAKGDMWAPCPFHQEKSASFHVDDRKG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
FYYCFGCQAKGDMFKFVQETENVGFMEAVQILAGEAGMQMPARDPQAQEKADKATQLAKV
EEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEAAVQHFRLQLKTQAASDARAYLEGRGMDQATLDRFEIGFAPNGRNSTHQALVDKGFAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCCCCHHHHHHHCCCCC
DDITAANVSTKPDDGGAPYDAFRDRIIFPIRDARGRCLGFGGRAMDPNARAKYLNSRETP
CCCEECCCCCCCCCCCCCHHHHHCCEEEEEECCCCCEEECCCCCCCCCHHHHHCCCCCCC
LFDKGRALYNHGPAREAAGKGQPLIVAEGYMDVIALVGAGFEAAVAPLGTAVTEDQLRLM
CCCCCCHHHCCCCCHHHCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHEE
WRITDEPIIALDGDKAGLRAAMRLVDLAMPLLEGGKSLRFVILPEGQDPDDLIRAQGPGA
EEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCHH
MQALLDKAEPMVTLLWRRETEGQVFDSPERRSALDGRLRQALQRIGDPGLRRHYGDAISE
HHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRRALFRPQQKPWQPGKRGPWKGRGFAEAQAPLAATRARVGALSGETLRTGLIVATCIRF
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
PDLIDVFEPQLDRMNPRDPDHARLTAHLLRTQNREAATLLDEINASPLAPALESLLRLSH
HHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
LAITPFLIGAGDRARAEDCLEEEFARLTAARAIEDEMVEASQDVEAAPDEGDPALAYRMK
HHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
EALRGKHAAQSPKGEDNAEQFGETSEARSAFHQLLSTAGQGKKLRK
HHHCCCHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]