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The map label for this gene is rihA [H]

Identifier: 89055731

GI number: 89055731

Start: 3288702

End: 3289610

Strand: Direct

Name: rihA [H]

Synonym: Jann_3240

Alternate gene names: 89055731

Gene position: 3288702-3289610 (Clockwise)

Preceding gene: 89055730

Following gene: 89055733

Centisome position: 76.16

GC content: 65.9

Gene sequence:

>909_bases
ATGACACGTCCGGTGTTGATCGACACCGATCCCGGGCTCGACGACGCTGTGGGCATTCTGATGGCGCTGGCCGATCCGCG
ACTGGACGTTCGCGCCGTAACCTCCGTCGCGGGCAATATCGGGATCGCGACCACCACCCGAAATGTCGGGCACCTTCTGG
CGGCAGCAGGGCGTGACGACATCGCTTATGCAGCCGGTGCAGCGGGACCATTGACGGGCGATGAGCTAAGCGAAGAGGCG
ATCCATGGCGCAGACGGGCTTGGGGGTGTGACGCTGCCTGATCCGCTGAAAAAACCTGATCCGGGAGGGGCCGTATCGCT
ATTGGCAGAGCGATTGCTGGACGCGCCGGAGGGGACAGTGACGATCCTCGCCCTCGGCCCGCTCACCAATCTGGCGCTTC
TGTCCCGGGATGCGCCGGAGGCCTATGGCCGGATCAGCCGGATCATCGCCATGGGCGGCACGATCTATCAGCCCGGCAAC
GTCGGACCGCACACGGAGTTCAACATGGCCGCCGATCCGATGGCGGCGCAGATGGTATTCCACGGGCCGGTGCCGGTCAC
GCTGATCCCGCTGGACGTGACCCGCAAGCTGCGCGCAACGCCCGCGGATCTTGACCGCCTCGCCGCATGTGGTGCACCCG
CCGCCACCCTCGCGGCAGACCTCATCCGGGCCTATTTTGCGGGGAACACTGACCGCACCTCGCGCCCGCTTCACGACCCC
TGCGTCATGCTGATGGCCGTGGTCCCGCAATTGTTCGGGACGCACCCCCTGCGGCTTACTGTCGATCAAGACCACTATCC
GGGACGTCTGGTCCAATCGCCGGACGGGTCCGCCATAGATGTGGCGATGACCATCGACGCGGCCGCCGCGCTGGATATGC
TGTGGCACGGCCTTGGGACAGCGACTTAG

Upstream 100 bases:

>100_bases
GCCCGAACGAGAAGTACGACCTGTCCAGCTTCACCGGCGGCATCCGGTCGTTCGTCCGCGTGCTGGCGGCCCTTTCTGAG
ATTGAGGCGGCCGCTGCCCC

Downstream 100 bases:

>100_bases
TCGGTCAGGTCCATCTCCACCAACCGGGTCCAGAAGGCGATGCCAGGCACGATGGCGTCATCGCAGAAATCGTATCCGGT
GTTGTGATGCAACGCTCCGT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTRPVLIDTDPGLDDAVGILMALADPRLDVRAVTSVAGNIGIATTTRNVGHLLAAAGRDDIAYAAGAAGPLTGDELSEEA
IHGADGLGGVTLPDPLKKPDPGGAVSLLAERLLDAPEGTVTILALGPLTNLALLSRDAPEAYGRISRIIAMGGTIYQPGN
VGPHTEFNMAADPMAAQMVFHGPVPVTLIPLDVTRKLRATPADLDRLAACGAPAATLAADLIRAYFAGNTDRTSRPLHDP
CVMLMAVVPQLFGTHPLRLTVDQDHYPGRLVQSPDGSAIDVAMTIDAAAALDMLWHGLGTAT

Sequences:

>Translated_302_residues
MTRPVLIDTDPGLDDAVGILMALADPRLDVRAVTSVAGNIGIATTTRNVGHLLAAAGRDDIAYAAGAAGPLTGDELSEEA
IHGADGLGGVTLPDPLKKPDPGGAVSLLAERLLDAPEGTVTILALGPLTNLALLSRDAPEAYGRISRIIAMGGTIYQPGN
VGPHTEFNMAADPMAAQMVFHGPVPVTLIPLDVTRKLRATPADLDRLAACGAPAATLAADLIRAYFAGNTDRTSRPLHDP
CVMLMAVVPQLFGTHPLRLTVDQDHYPGRLVQSPDGSAIDVAMTIDAAAALDMLWHGLGTAT
>Mature_301_residues
TRPVLIDTDPGLDDAVGILMALADPRLDVRAVTSVAGNIGIATTTRNVGHLLAAAGRDDIAYAAGAAGPLTGDELSEEAI
HGADGLGGVTLPDPLKKPDPGGAVSLLAERLLDAPEGTVTILALGPLTNLALLSRDAPEAYGRISRIIAMGGTIYQPGNV
GPHTEFNMAADPMAAQMVFHGPVPVTLIPLDVTRKLRATPADLDRLAACGAPAATLAADLIRAYFAGNTDRTSRPLHDPC
VMLMAVVPQLFGTHPLRLTVDQDHYPGRLVQSPDGSAIDVAMTIDAAAALDMLWHGLGTAT

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=256, Percent_Identity=41.796875, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1786213, Length=262, Percent_Identity=38.1679389312977, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1788486, Length=304, Percent_Identity=31.25, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32565474, Length=201, Percent_Identity=32.8358208955224, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17565698, Length=199, Percent_Identity=29.6482412060301, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320608, Length=267, Percent_Identity=28.4644194756554, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24641839, Length=210, Percent_Identity=32.3809523809524, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583915, Length=207, Percent_Identity=28.9855072463768, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24641837, Length=277, Percent_Identity=28.8808664259928, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 31184; Mature: 31053

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPVLIDTDPGLDDAVGILMALADPRLDVRAVTSVAGNIGIATTTRNVGHLLAAAGRDD
CCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCC
IAYAAGAAGPLTGDELSEEAIHGADGLGGVTLPDPLKKPDPGGAVSLLAERLLDAPEGTV
CEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCE
TILALGPLTNLALLSRDAPEAYGRISRIIAMGGTIYQPGNVGPHTEFNMAADPMAAQMVF
EEEEECCHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHEEE
HGPVPVTLIPLDVTRKLRATPADLDRLAACGAPAATLAADLIRAYFAGNTDRTSRPLHDP
ECCCCEEEEECCHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CVMLMAVVPQLFGTHPLRLTVDQDHYPGRLVQSPDGSAIDVAMTIDAAAALDMLWHGLGT
HHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHCCC
AT
CC
>Mature Secondary Structure 
TRPVLIDTDPGLDDAVGILMALADPRLDVRAVTSVAGNIGIATTTRNVGHLLAAAGRDD
CCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHHHHHHHCCCCC
IAYAAGAAGPLTGDELSEEAIHGADGLGGVTLPDPLKKPDPGGAVSLLAERLLDAPEGTV
CEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCE
TILALGPLTNLALLSRDAPEAYGRISRIIAMGGTIYQPGNVGPHTEFNMAADPMAAQMVF
EEEEECCHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHEEE
HGPVPVTLIPLDVTRKLRATPADLDRLAACGAPAATLAADLIRAYFAGNTDRTSRPLHDP
ECCCCEEEEECCHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CVMLMAVVPQLFGTHPLRLTVDQDHYPGRLVQSPDGSAIDVAMTIDAAAALDMLWHGLGT
HHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEEEEEEHHHHHHHHHHHHHCCC
AT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA