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The map label for this gene is rhtB [H]

Identifier: 89055591

GI number: 89055591

Start: 3136458

End: 3137096

Strand: Direct

Name: rhtB [H]

Synonym: Jann_3100

Alternate gene names: 89055591

Gene position: 3136458-3137096 (Clockwise)

Preceding gene: 89055590

Following gene: 89055592

Centisome position: 72.64

GC content: 63.22

Gene sequence:

>639_bases
ATGTTCGAGGTCTCATTGCCCTTCATCGCCTACGTTGTCGCATTGGGTGTCGCGGCGGCGATCCCCGGACCCGGCGTTGC
CGCGCTGGTCGGGCGGTCGCTAGCGCAAGGCGCGCGTACCTCTCTGCCGTTCATCATCGGCCTCGCGTTAGGCGATGTCG
TGTTCCTCACAATCGCCATTCTCGGCCTCGCAACACTTGCGAACACTGCGGCGGGTCTGTTCCTCGTCGTAAAGATCATG
GGTGCGCTCTACCTGCTCTACCTCGGATGGCGGTTCTGGACCGCTGAAATTGCGCGCATCGACCTTGCAGGACGTCCACC
AATCGGTTCGTGGTCAGGCGCAGTCGCCGGTCTGGCCGTGACCCTTGGCAACCCCAAAACGGTGGTGTTCTACCTCGCGC
TTGTTCCCAACGTCATTGACCTTGAACAGGTCGGCCTCTCCAGCTGGCCCATCCTGAGTGTACTGACGATACTTGTGCTG
CTTGCCGTCCTGATCCCCTATGCAGCCCTCGCGGATCGGTTGCGGCAATTCTTCGCCAGCAGCCGTGCGCGCCGTCGCCT
CAACCGCGGGGCGGCCATGGCCATCGGCGGGGCGGGCATCGCGATCCTGTCCGACGCGGCAATTGAACTGCGACGCTAG

Upstream 100 bases:

>100_bases
TTTCGGCAGCGACGGCAGGGCCAAATCCTCCCTCAGCGACGCGATGCAGAACAGATCGCCATCGACCCGTGGCCAATTGG
CAGAGCCGGAGTAGCGTGAC

Downstream 100 bases:

>100_bases
CGACGGCGGGATTTTTGCGACCAAACACGGCGGATCTGCGTTCATATCAAAGGAGAGCGAGATGACACGACCCCTTGCTG
CACCCCGCCAAAGACTGAGG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MFEVSLPFIAYVVALGVAAAIPGPGVAALVGRSLAQGARTSLPFIIGLALGDVVFLTIAILGLATLANTAAGLFLVVKIM
GALYLLYLGWRFWTAEIARIDLAGRPPIGSWSGAVAGLAVTLGNPKTVVFYLALVPNVIDLEQVGLSSWPILSVLTILVL
LAVLIPYAALADRLRQFFASSRARRRLNRGAAMAIGGAGIAILSDAAIELRR

Sequences:

>Translated_212_residues
MFEVSLPFIAYVVALGVAAAIPGPGVAALVGRSLAQGARTSLPFIIGLALGDVVFLTIAILGLATLANTAAGLFLVVKIM
GALYLLYLGWRFWTAEIARIDLAGRPPIGSWSGAVAGLAVTLGNPKTVVFYLALVPNVIDLEQVGLSSWPILSVLTILVL
LAVLIPYAALADRLRQFFASSRARRRLNRGAAMAIGGAGIAILSDAAIELRR
>Mature_212_residues
MFEVSLPFIAYVVALGVAAAIPGPGVAALVGRSLAQGARTSLPFIIGLALGDVVFLTIAILGLATLANTAAGLFLVVKIM
GALYLLYLGWRFWTAEIARIDLAGRPPIGSWSGAVAGLAVTLGNPKTVVFYLALVPNVIDLEQVGLSSWPILSVLTILVL
LAVLIPYAALADRLRQFFASSRARRRLNRGAAMAIGGAGIAILSDAAIELRR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=183, Percent_Identity=32.7868852459016, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22148; Mature: 22148

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVSLPFIAYVVALGVAAAIPGPGVAALVGRSLAQGARTSLPFIIGLALGDVVFLTIAI
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LGLATLANTAAGLFLVVKIMGALYLLYLGWRFWTAEIARIDLAGRPPIGSWSGAVAGLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHEE
TLGNPKTVVFYLALVPNVIDLEQVGLSSWPILSVLTILVLLAVLIPYAALADRLRQFFAS
ECCCCCHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRARRRLNRGAAMAIGGAGIAILSDAAIELRR
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFEVSLPFIAYVVALGVAAAIPGPGVAALVGRSLAQGARTSLPFIIGLALGDVVFLTIAI
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LGLATLANTAAGLFLVVKIMGALYLLYLGWRFWTAEIARIDLAGRPPIGSWSGAVAGLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHEE
TLGNPKTVVFYLALVPNVIDLEQVGLSSWPILSVLTILVLLAVLIPYAALADRLRQFFAS
ECCCCCHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRARRRLNRGAAMAIGGAGIAILSDAAIELRR
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]