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The map label for this gene is rbsB [H]

Identifier: 89055578

GI number: 89055578

Start: 3121307

End: 3122338

Strand: Direct

Name: rbsB [H]

Synonym: Jann_3087

Alternate gene names: 89055578

Gene position: 3121307-3122338 (Clockwise)

Preceding gene: 89055577

Following gene: 89055579

Centisome position: 72.29

GC content: 59.5

Gene sequence:

>1032_bases
ATGAAACGTTTCATATTGACAGATCACAGCGGGTCTGACTTCCTGACCCGTGCCAAAGGTCCACGCCGGGAGGGCGCGAC
CAAGGCACCAAATATGAATTGGGAGGCTCTTATGAAGCTCTTGAAAAACACCGCCGCGGCGGTGACCGCTCTCGCTTTGT
CTGTAGGTATGGCGGCAGCCCAGGATCAACCGACCGTTGGCCTGATTATGAAGTCGCTTGCCAACGAATTCTTCCAGAAC
ATGATGGTTGGGGCGGAAGCCCATGCCGAAGAACGCGGCGATTACGAATTGCTGGCCGTCGGAATGCAGAACGAAACCGA
CTTCGAGTCGCAGATCAACGCGGTGGAGAACTTCATCACCCAGGGCGTTGATGCCATCGTTGTCGCCCCGGCGGATTCGC
GCGCCATGGTACGGCCCCTGCGCCGCGCGATGGAGGCAGGCATCACCGTGATCAACTTCGACGTGGCGCTGGACACCGGC
GCGATGGAACAGCAAGGCATTGAGCTTGCCTTTGTTGGGCCCGACAACCGGATGGGTGCAGAGATGGCGGGCAACGCCCT
TGGGGAAGCTCTTGGCGAAGGCGGGCGTGTTGTCATCATCGAAGGCAACCCGGGCGCGGATAACGCAACTCAGCGGCGCT
TGGGGTTTGAAGATGCAATTGCCACCCACGGTCTTGAACTTCTCGAAAGCCGCACCGCCCATTGGGAAACGGAAGAAGCC
AATCAGGTGTTCGCTGCGATGCTGACGGCGCATCCGGATCTACAAGGTGTGATGGCGGCAAACGATTCGATGGCGATCGG
TGTGATCGAAGCGCTGGATGCCGCGGGCCGCGATGACATTCTTGTCGTTGGCTTCGACGCCGTCCCCGCCGTTCTGCCAA
TGATAGAAGATGGCCGGATGTTGGCCACCGTGGATCAGTTCGGGCAGGCCATGGCAGCCAACGCCATCGACCTGGCGCTG
GAGGTGCTTGAAACGGGCGTGGATCTTGAAGGTTGGGTCCGCACGCCGATCGAGCTTGTGACAGCCAACTAA

Upstream 100 bases:

>100_bases
TCGACGTGTCGCCGGACGAGCGGCGGCAGCCCCGAATTGACGCCTGACGAACGATCGTTTTCCATGTGGACCAACAGGAA
TTCGCATCTCCTCCAAAAAA

Downstream 100 bases:

>100_bases
CGACCACGACGACCCATCTCTCCCTGCGACCAGGCAGGTGTCCGCCGCATCCGGCGGGCACCGGTCTGCACAACCGGACT
GCTTACCATGCGGCAATCTT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKRFILTDHSGSDFLTRAKGPRREGATKAPNMNWEALMKLLKNTAAAVTALALSVGMAAAQDQPTVGLIMKSLANEFFQN
MMVGAEAHAEERGDYELLAVGMQNETDFESQINAVENFITQGVDAIVVAPADSRAMVRPLRRAMEAGITVINFDVALDTG
AMEQQGIELAFVGPDNRMGAEMAGNALGEALGEGGRVVIIEGNPGADNATQRRLGFEDAIATHGLELLESRTAHWETEEA
NQVFAAMLTAHPDLQGVMAANDSMAIGVIEALDAAGRDDILVVGFDAVPAVLPMIEDGRMLATVDQFGQAMAANAIDLAL
EVLETGVDLEGWVRTPIELVTAN

Sequences:

>Translated_343_residues
MKRFILTDHSGSDFLTRAKGPRREGATKAPNMNWEALMKLLKNTAAAVTALALSVGMAAAQDQPTVGLIMKSLANEFFQN
MMVGAEAHAEERGDYELLAVGMQNETDFESQINAVENFITQGVDAIVVAPADSRAMVRPLRRAMEAGITVINFDVALDTG
AMEQQGIELAFVGPDNRMGAEMAGNALGEALGEGGRVVIIEGNPGADNATQRRLGFEDAIATHGLELLESRTAHWETEEA
NQVFAAMLTAHPDLQGVMAANDSMAIGVIEALDAAGRDDILVVGFDAVPAVLPMIEDGRMLATVDQFGQAMAANAIDLAL
EVLETGVDLEGWVRTPIELVTAN
>Mature_343_residues
MKRFILTDHSGSDFLTRAKGPRREGATKAPNMNWEALMKLLKNTAAAVTALALSVGMAAAQDQPTVGLIMKSLANEFFQN
MMVGAEAHAEERGDYELLAVGMQNETDFESQINAVENFITQGVDAIVVAPADSRAMVRPLRRAMEAGITVINFDVALDTG
AMEQQGIELAFVGPDNRMGAEMAGNALGEALGEGGRVVIIEGNPGADNATQRRLGFEDAIATHGLELLESRTAHWETEEA
NQVFAAMLTAHPDLQGVMAANDSMAIGVIEALDAAGRDDILVVGFDAVPAVLPMIEDGRMLATVDQFGQAMAANAIDLAL
EVLETGVDLEGWVRTPIELVTAN

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=285, Percent_Identity=32.6315789473684, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1790526, Length=293, Percent_Identity=32.0819112627986, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790674, Length=245, Percent_Identity=31.8367346938775, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788473, Length=291, Percent_Identity=26.4604810996564, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788898, Length=325, Percent_Identity=23.3846153846154, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36463; Mature: 36463

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFILTDHSGSDFLTRAKGPRREGATKAPNMNWEALMKLLKNTAAAVTALALSVGMAAA
CCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QDQPTVGLIMKSLANEFFQNMMVGAEAHAEERGDYELLAVGMQNETDFESQINAVENFIT
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
QGVDAIVVAPADSRAMVRPLRRAMEAGITVINFDVALDTGAMEQQGIELAFVGPDNRMGA
CCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHCCCEEEEECCCCCCCH
EMAGNALGEALGEGGRVVIIEGNPGADNATQRRLGFEDAIATHGLELLESRTAHWETEEA
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHH
NQVFAAMLTAHPDLQGVMAANDSMAIGVIEALDAAGRDDILVVGFDAVPAVLPMIEDGRM
HHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCE
LATVDQFGQAMAANAIDLALEVLETGVDLEGWVRTPIELVTAN
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEECC
>Mature Secondary Structure
MKRFILTDHSGSDFLTRAKGPRREGATKAPNMNWEALMKLLKNTAAAVTALALSVGMAAA
CCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QDQPTVGLIMKSLANEFFQNMMVGAEAHAEERGDYELLAVGMQNETDFESQINAVENFIT
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
QGVDAIVVAPADSRAMVRPLRRAMEAGITVINFDVALDTGAMEQQGIELAFVGPDNRMGA
CCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHCCCEEEEECCCCCCCH
EMAGNALGEALGEGGRVVIIEGNPGADNATQRRLGFEDAIATHGLELLESRTAHWETEEA
HHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHH
NQVFAAMLTAHPDLQGVMAANDSMAIGVIEALDAAGRDDILVVGFDAVPAVLPMIEDGRM
HHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCE
LATVDQFGQAMAANAIDLALEVLETGVDLEGWVRTPIELVTAN
EEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]