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The map label for this gene is nasA [H]

Identifier: 89055536

GI number: 89055536

Start: 3073343

End: 3075964

Strand: Direct

Name: nasA [H]

Synonym: Jann_3045

Alternate gene names: 89055536

Gene position: 3073343-3075964 (Clockwise)

Preceding gene: 89055535

Following gene: 89055537

Centisome position: 71.18

GC content: 67.2

Gene sequence:

>2622_bases
ATGGACGGGCCGGTGCGGGAGGCGGAGGTGCGCTCCACCTGCCCCTATTGCGGGGTCGGCTGCGGCGTATTGCTGCGCCC
GGACGGCGCCGGAGGTTTGGCGGTGAGGGGAGATCCGGAACATCCGGCGAACTACGGGAAGTTGTGTTCCAAGGGCACCG
CGTTGGGCGAGACGGTGGGGCTTGACGGGCGGTTACGTGCGCCAATGGTGGGCGGGCAAGAGACGTCCTGGGACACCGCA
TTGGATCTGGTCGCCACACGCTTCAACGACACGATCGCAAAGCACGGCCCTGACAGCACCGCCTTCTACGTCTCGGGCCA
ATTGCTGACAGAGGATTACTACGTCGCCAACAAGCTGATGAAGGGTTTCATCGGGTCCGCCAACATCGACACGAACTCCA
GGCTCTGCATGGCGTCCACCGTCGCGGGCCACAAACGCGCGTTTGGCACGGACACGGTTCCGGGCACCTATGCGGATCTG
GATGAGGCCGATCTGGTGGTGCTTGTGGGCTCCAACCTCGCGTGGTGCCACCCCGTGCTGTACCAGCGCGTAAAGGCGTC
CGGCGCGAAGATCATCGTCGTGGACCCCCGCCGCACGGCGAGCTGTGACGGCGCGGATCTGCACCTCGCCATCGCGCCCG
GCTCGGACGTCGCCCTGTTCAACGGGCTCCTCGCCGCTATCGCCAATAAAGGCGCGCTCGACCCCGATTTCCTGAACCAC
ACCAACGGCCTAGACGCCGCCCTCCAATCCGCGCGCAACGACACCGTGGCGAGGACGGGCCTTAGACCGGAGGAGCGGGC
GCGGTTCTATGACATGTGGATCGGCACCCAAAAGGTCGTCACCATCTTCAGCCAGGGCGTCAACCAATCCACCTCGGGCA
GCGACAAAGTCAACGCGATCCTCAACTGCCATCTCGCCACGGGTCGCATCGGCAAGCCCGGCATGGGGCCCTTCAGCGTC
ACCGGGCAACCCAACGCCATGGGTGGGCGCGAGGTCGGGGGGCTCGCCAACATGCTCGCCTGCCATCTGGATCTGGACAA
CGCCGAGCACCGTGCTGCCGTGCGCGGGTTCTGGCAGGCCCCCCATATGCCCGACGCGCCCGGCCTGAAGGCTGTCGATA
TGTTCCATGCCGTGGGCGACGGGCGCATCAAGGCGCTGTGGATCATCCATACGAACCCCGCCGTTTCCATGCCCGAGGCC
GACGCCGTCCGGGACGCCATCGCGGGCTGCGACTTTGTCGTCGTCAGCGACGTGACCGGCGCCGCGGATACCGCGCGCCT
GGCCGACGTGCTGCTGCCCGCGACGGCCTGGGCCGAGAAAGACGGCACCGTCACCAATTCCGACCGCACCATCAGCCGCC
AGCGCGCGGTGCTGTCCGCGCCTCCTAACGCGCGGCCCGATTGGGATATTTTGGCCGACGTCGGACGGCGCATGGGCCAT
GTCCGCGCCTTTGACTATGCCTCGCCTGCGGAGATCTTTCGCGAATACGCTGCTCTCTCCGGCGTCGCCGCGCGCTTTGG
GCGGGACTTCGATATCTCGGGCCTCAGCGATCTGGATGACACGGGCTACGCCGACTTTGCGCCGACACGCTGGCCGATCA
GCGCCTCCCGCCAAGGGGGGCGGTTTTTCGCGGATGGGCAATTCTTCCACCCCGACGGCAAGGCGCGTTTTCTCCCCGTC
ACAACCCGCGCCCCTGCCGCGATGCCGACGCAGGCCTATCCGTTCCGGCTCAACACGGGCCGTGTGCGCGACCAATGGCA
CACGATGACACGCACCGGGTTGAGCCCGCGCCTGAGCGGTCATCTGGCGGAACCTTACCTCGATATCCACCCCAAGGATG
CCACGCGCCTGGGTCTGTCTGCCGCCAATCTGGCGCAGGTCACCAGCGCTCATGGCACCGCCATCCTGCGCGTAAGGATC
ACCGATGACGTCCGCCCCGGCCAGCTGTTCGCACCAATGCATTGGACGGCTGCGACTGCGCCCACGGGCCGGATCGACGC
GCTGGTTGCCGCCGCCACGGACCCCGTCTCGGGCCAGCCGGAAAGCAAGGCCGCCGTTGTCTCGGTCCAGCGGATGCAGG
CCGCGTGGTATGGCTTTGCCGTCTCCTCCACGTGGATGCGGCCCGAGTGCGATTATTGGGCCGTGGCCCCGACCGCGACG
GGACACCGCGTCGAGTTGGCAGGCCGCTACACGCCCGAGGATTGGGAGGCCTTCGCCCGCGCCCTGTTCGACTTGCCCCA
CGCCACGGTCACGTCGCTGTTGGACCCCGCCCGCGGCACCGCCCGGATCGCGTTTGAAAGCGAGGGCCGCACGCTGGCCG
CGCTGTTCGTGTCGCCCGAGCCCGTGGCCGTCCTGCGCGACTATCTGGCGACGTTGCCCGGAACGCCCGCGCCGTCGCCT
TTGTCAGGGCGGCCCCCGGCGGGTGTCCCCGATCCAGGAGCGACGCTGTGCGCCTGTTTCGGAATCGGCATCAAAACGAT
CACATCGGCCATTGAAAACAACGCCTTACTAAGTGTGGAGGCCATCGGAGACGCGTTGCAGGCCGGCACCAATTGCGGGT
CGTGCAGGCCGGAACTGGCGGCGTTGCTGGCGAAGACACAACCCCATGAGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
CAAAGGGCGAAGACGGCCGGATCGACACGTACCCGGTGAGGGTCGAGGCCGGGCGTATCCTGATTGACGCGCGCACCCTG
GCTGCGCGGAGTGCGGCGTG

Downstream 100 bases:

>100_bases
GCCTTGCGCCTTACGTCGCCACGCTAGGGCGGGGTCCGGGGCGGTCTCGGTCCTTGACCCGTGATGAGGCGGCGGCGGCG
ATGGAGATTATGCTCTCGGG

Product: assimilatory nitrate reductase (NADH) subunit alpha apoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 873; Mature: 873

Protein sequence:

>873_residues
MDGPVREAEVRSTCPYCGVGCGVLLRPDGAGGLAVRGDPEHPANYGKLCSKGTALGETVGLDGRLRAPMVGGQETSWDTA
LDLVATRFNDTIAKHGPDSTAFYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASTVAGHKRAFGTDTVPGTYADL
DEADLVVLVGSNLAWCHPVLYQRVKASGAKIIVVDPRRTASCDGADLHLAIAPGSDVALFNGLLAAIANKGALDPDFLNH
TNGLDAALQSARNDTVARTGLRPEERARFYDMWIGTQKVVTIFSQGVNQSTSGSDKVNAILNCHLATGRIGKPGMGPFSV
TGQPNAMGGREVGGLANMLACHLDLDNAEHRAAVRGFWQAPHMPDAPGLKAVDMFHAVGDGRIKALWIIHTNPAVSMPEA
DAVRDAIAGCDFVVVSDVTGAADTARLADVLLPATAWAEKDGTVTNSDRTISRQRAVLSAPPNARPDWDILADVGRRMGH
VRAFDYASPAEIFREYAALSGVAARFGRDFDISGLSDLDDTGYADFAPTRWPISASRQGGRFFADGQFFHPDGKARFLPV
TTRAPAAMPTQAYPFRLNTGRVRDQWHTMTRTGLSPRLSGHLAEPYLDIHPKDATRLGLSAANLAQVTSAHGTAILRVRI
TDDVRPGQLFAPMHWTAATAPTGRIDALVAAATDPVSGQPESKAAVVSVQRMQAAWYGFAVSSTWMRPECDYWAVAPTAT
GHRVELAGRYTPEDWEAFARALFDLPHATVTSLLDPARGTARIAFESEGRTLAALFVSPEPVAVLRDYLATLPGTPAPSP
LSGRPPAGVPDPGATLCACFGIGIKTITSAIENNALLSVEAIGDALQAGTNCGSCRPELAALLAKTQPHEAAE

Sequences:

>Translated_873_residues
MDGPVREAEVRSTCPYCGVGCGVLLRPDGAGGLAVRGDPEHPANYGKLCSKGTALGETVGLDGRLRAPMVGGQETSWDTA
LDLVATRFNDTIAKHGPDSTAFYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASTVAGHKRAFGTDTVPGTYADL
DEADLVVLVGSNLAWCHPVLYQRVKASGAKIIVVDPRRTASCDGADLHLAIAPGSDVALFNGLLAAIANKGALDPDFLNH
TNGLDAALQSARNDTVARTGLRPEERARFYDMWIGTQKVVTIFSQGVNQSTSGSDKVNAILNCHLATGRIGKPGMGPFSV
TGQPNAMGGREVGGLANMLACHLDLDNAEHRAAVRGFWQAPHMPDAPGLKAVDMFHAVGDGRIKALWIIHTNPAVSMPEA
DAVRDAIAGCDFVVVSDVTGAADTARLADVLLPATAWAEKDGTVTNSDRTISRQRAVLSAPPNARPDWDILADVGRRMGH
VRAFDYASPAEIFREYAALSGVAARFGRDFDISGLSDLDDTGYADFAPTRWPISASRQGGRFFADGQFFHPDGKARFLPV
TTRAPAAMPTQAYPFRLNTGRVRDQWHTMTRTGLSPRLSGHLAEPYLDIHPKDATRLGLSAANLAQVTSAHGTAILRVRI
TDDVRPGQLFAPMHWTAATAPTGRIDALVAAATDPVSGQPESKAAVVSVQRMQAAWYGFAVSSTWMRPECDYWAVAPTAT
GHRVELAGRYTPEDWEAFARALFDLPHATVTSLLDPARGTARIAFESEGRTLAALFVSPEPVAVLRDYLATLPGTPAPSP
LSGRPPAGVPDPGATLCACFGIGIKTITSAIENNALLSVEAIGDALQAGTNCGSCRPELAALLAKTQPHEAAE
>Mature_873_residues
MDGPVREAEVRSTCPYCGVGCGVLLRPDGAGGLAVRGDPEHPANYGKLCSKGTALGETVGLDGRLRAPMVGGQETSWDTA
LDLVATRFNDTIAKHGPDSTAFYVSGQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASTVAGHKRAFGTDTVPGTYADL
DEADLVVLVGSNLAWCHPVLYQRVKASGAKIIVVDPRRTASCDGADLHLAIAPGSDVALFNGLLAAIANKGALDPDFLNH
TNGLDAALQSARNDTVARTGLRPEERARFYDMWIGTQKVVTIFSQGVNQSTSGSDKVNAILNCHLATGRIGKPGMGPFSV
TGQPNAMGGREVGGLANMLACHLDLDNAEHRAAVRGFWQAPHMPDAPGLKAVDMFHAVGDGRIKALWIIHTNPAVSMPEA
DAVRDAIAGCDFVVVSDVTGAADTARLADVLLPATAWAEKDGTVTNSDRTISRQRAVLSAPPNARPDWDILADVGRRMGH
VRAFDYASPAEIFREYAALSGVAARFGRDFDISGLSDLDDTGYADFAPTRWPISASRQGGRFFADGQFFHPDGKARFLPV
TTRAPAAMPTQAYPFRLNTGRVRDQWHTMTRTGLSPRLSGHLAEPYLDIHPKDATRLGLSAANLAQVTSAHGTAILRVRI
TDDVRPGQLFAPMHWTAATAPTGRIDALVAAATDPVSGQPESKAAVVSVQRMQAAWYGFAVSSTWMRPECDYWAVAPTAT
GHRVELAGRYTPEDWEAFARALFDLPHATVTSLLDPARGTARIAFESEGRTLAALFVSPEPVAVLRDYLATLPGTPAPSP
LSGRPPAGVPDPGATLCACFGIGIKTITSAIENNALLSVEAIGDALQAGTNCGSCRPELAALLAKTQPHEAAE

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=802, Percent_Identity=28.6783042394015, Blast_Score=284, Evalue=1e-77,
Organism=Escherichia coli, GI3868721, Length=706, Percent_Identity=28.6118980169972, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI3868720, Length=262, Percent_Identity=30.9160305343511, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI3868719, Length=265, Percent_Identity=29.0566037735849, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1787778, Length=375, Percent_Identity=24.8, Blast_Score=98, Evalue=3e-21,
Organism=Escherichia coli, GI1787870, Length=541, Percent_Identity=22.9205175600739, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92524; Mature: 92524

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGPVREAEVRSTCPYCGVGCGVLLRPDGAGGLAVRGDPEHPANYGKLCSKGTALGETVG
CCCCCCCHHHHHCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCHHHHHHCCCCCCHHCC
LDGRLRAPMVGGQETSWDTALDLVATRFNDTIAKHGPDSTAFYVSGQLLTEDYYVANKLM
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECCHHHHHHHHH
KGFIGSANIDTNSRLCMASTVAGHKRAFGTDTVPGTYADLDEADLVVLVGSNLAWCHPVL
HHHCCCCCCCCCCCEEEEEHHCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
YQRVKASGAKIIVVDPRRTASCDGADLHLAIAPGSDVALFNGLLAAIANKGALDPDFLNH
HHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHCC
TNGLDAALQSARNDTVARTGLRPEERARFYDMWIGTQKVVTIFSQGVNQSTSGSDKVNAI
CCCHHHHHHHHCCCCHHHCCCCHHHHHEEEHHHCCHHHHHHHHHHCCCCCCCCCCCEEEE
LNCHLATGRIGKPGMGPFSVTGQPNAMGGREVGGLANMLACHLDLDNAEHRAAVRGFWQA
EEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHCC
PHMPDAPGLKAVDMFHAVGDGRIKALWIIHTNPAVSMPEADAVRDAIAGCDFVVVSDVTG
CCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCC
AADTARLADVLLPATAWAEKDGTVTNSDRTISRQRAVLSAPPNARPDWDILADVGRRMGH
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEECCCCCCCCHHHHHHHHHHHCC
VRAFDYASPAEIFREYAALSGVAARFGRDFDISGLSDLDDTGYADFAPTRWPISASRQGG
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RFFADGQFFHPDGKARFLPVTTRAPAAMPTQAYPFRLNTGRVRDQWHTMTRTGLSPRLSG
EEEECCEEECCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCC
HLAEPYLDIHPKDATRLGLSAANLAQVTSAHGTAILRVRITDDVRPGQLFAPMHWTAATA
CCCCCEEECCCCCHHHHCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEECCCEEEECCC
PTGRIDALVAAATDPVSGQPESKAAVVSVQRMQAAWYGFAVSSTWMRPECDYWAVAPTAT
CCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCC
GHRVELAGRYTPEDWEAFARALFDLPHATVTSLLDPARGTARIAFESEGRTLAALFVSPE
CCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCC
PVAVLRDYLATLPGTPAPSPLSGRPPAGVPDPGATLCACFGIGIKTITSAIENNALLSVE
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEHH
AIGDALQAGTNCGSCRPELAALLAKTQPHEAAE
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDGPVREAEVRSTCPYCGVGCGVLLRPDGAGGLAVRGDPEHPANYGKLCSKGTALGETVG
CCCCCCCHHHHHCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCHHHHHHCCCCCCHHCC
LDGRLRAPMVGGQETSWDTALDLVATRFNDTIAKHGPDSTAFYVSGQLLTEDYYVANKLM
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECCHHHHHHHHH
KGFIGSANIDTNSRLCMASTVAGHKRAFGTDTVPGTYADLDEADLVVLVGSNLAWCHPVL
HHHCCCCCCCCCCCEEEEEHHCCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
YQRVKASGAKIIVVDPRRTASCDGADLHLAIAPGSDVALFNGLLAAIANKGALDPDFLNH
HHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHCC
TNGLDAALQSARNDTVARTGLRPEERARFYDMWIGTQKVVTIFSQGVNQSTSGSDKVNAI
CCCHHHHHHHHCCCCHHHCCCCHHHHHEEEHHHCCHHHHHHHHHHCCCCCCCCCCCEEEE
LNCHLATGRIGKPGMGPFSVTGQPNAMGGREVGGLANMLACHLDLDNAEHRAAVRGFWQA
EEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHHCC
PHMPDAPGLKAVDMFHAVGDGRIKALWIIHTNPAVSMPEADAVRDAIAGCDFVVVSDVTG
CCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCC
AADTARLADVLLPATAWAEKDGTVTNSDRTISRQRAVLSAPPNARPDWDILADVGRRMGH
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEECCCCCCCCHHHHHHHHHHHCC
VRAFDYASPAEIFREYAALSGVAARFGRDFDISGLSDLDDTGYADFAPTRWPISASRQGG
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RFFADGQFFHPDGKARFLPVTTRAPAAMPTQAYPFRLNTGRVRDQWHTMTRTGLSPRLSG
EEEECCEEECCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCC
HLAEPYLDIHPKDATRLGLSAANLAQVTSAHGTAILRVRITDDVRPGQLFAPMHWTAATA
CCCCCEEECCCCCHHHHCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEECCCEEEECCC
PTGRIDALVAAATDPVSGQPESKAAVVSVQRMQAAWYGFAVSSTWMRPECDYWAVAPTAT
CCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCC
GHRVELAGRYTPEDWEAFARALFDLPHATVTSLLDPARGTARIAFESEGRTLAALFVSPE
CCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCC
PVAVLRDYLATLPGTPAPSPLSGRPPAGVPDPGATLCACFGIGIKTITSAIENNALLSVE
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEHH
AIGDALQAGTNCGSCRPELAALLAKTQPHEAAE
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]