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The map label for this gene is nasD [H]

Identifier: 89055534

GI number: 89055534

Start: 3070582

End: 3073017

Strand: Direct

Name: nasD [H]

Synonym: Jann_3043

Alternate gene names: 89055534

Gene position: 3070582-3073017 (Clockwise)

Preceding gene: 89055533

Following gene: 89055535

Centisome position: 71.11

GC content: 63.01

Gene sequence:

>2436_bases
ATGGCCAAACAAAAGCTTGTCGTGATTGGTGCCGGCATGGCCTCGGGCCGGGTGCTGGAGCATCTGACAGACGCCGCGCC
GGATGCCTTTGACATCACGCTGTTCAACGCGGAGCCGCGCGGCAATTACAACCGCATCATGCTGTCCCCCGTGCTGGCGG
GCGATCAGACCTATGCCGACATCGTCACCCATGACGAGGCTTGGTACGCGGAACGGGCGATCACCTGCCGCTTTGGCGAA
AAGGTCTTGGAAATCGACCGGGACGCCAAGATCGTTGTCGGTGCCAACGGCCATGTGCCCTACGACCAGCTGATTTTCGG
CACTGGGTCGGACCCCTTCATGATCCCCCTGCCCGGCCATGACCTGGACGGCGTGATCGCCTACCGCGACCTGGAAGATA
CGGAGCGGATGATGGGCCTTCAACCGGGCCAAAAATGCGTCGTGATCGGCGGTGGCCTGCTGGGATTGGAAGCCGCCGCG
GGCATGGCCGCGCGCGGCGTGGACGTCACGGTTGTCCATATCATGGGCCACTTGATGGAGCGTCAGTTGGACGAGGCGGC
AGGCTACCTGCTGCGCAAGTCCCTGGAGGACAAGGGGATTACGATCTGCCTGCAAGCGAACTCCAAAGAGATCGTGGGCA
GGGATGGCCAGGTGAAAGCGCTGATGCTGGACGACGGCACGGAGCTGCCCTGCGATCTTCTGGTGATGGCCGTGGGCATC
CGCCCGTCCGTGGCGCTGGCCAAAACCGCCGGGTTGGCCGTGGGCCGAGGCATCCATGTGGATGACCAGATGGGCACGTC
CGACGGCGCGATCTTCGCGGTCGGGGAATGCGTGGAGCATAACGGCGCGATCTTCGGCCTTGTGGCGCCGCTTTATGACC
AAGCCAGAGTGCTGGCGCAGACCCTTTTGGGCGAGAAGGCCGCATTTGTGCAAAAGACGCTATCGACCAAGTTGAAGGTC
ACGGGCTGCGATCTGTTCAGCGCGGGCGACTTTGCCGATGGCGAGACCCGCGAGGATATCGTGTTCCGCGACCCCGCACG
CGGCATCTACAAGCGACTTGTTATAGAAGAAAACAGGCTCGTCGGCGCGGTCATGTACGGCGACACCGGCGACGGCAACT
GGTTCTTCCAACTGATCAAAGACGGCACGGACATCGAGGAGATGCGCGAGACGCTGATCTTCGGGCCTTCGATGCAGGGA
GGGCCCGCGCCGGACCCGCTCTCAGCCGTTGCAGCCTTGCCGCGTGATGCGGAGATCTGTGGCTGCAACGGCGTTTGCAA
AGGCATAATCGTGGACGCGATCGAGGGCGGCGCCGGCGATCTGGGCGCGGTACGGGGCGCGACGAAAGCCAGCGCGTCCT
GCGGCACATGTACGGGCTTGGTGGAGCAATTGCTGCAAGTCACCTTGGGTGACGGGTTCGAATTGCCGAGCGCGCAGCCG
ATCTGCGGCTGCTGCGATCTGACCCATGAGGACGTGCGGCGGCTGATCAAAACCACACCGCTGAAGTCGCAAGAGGCCGT
GTGGCAAGAGCTTGGGTGGAGCACCCGCAACGGCTGCCACGTCTGCCGCCCGGCGGTGAATTTCTACCTGCTGGCCGACT
GGCCGCTGGAGTATCAAGACGACCCCCAAAGCCGTTTCATCAACGAGCGAAAGCACGCAAATATCCAGAAAGACGGCACT
TTTTCCGTGGTGCCGCGCATGTGGGGCGGGATCACCAACCCGTCCGAGCTGCGCGCGATTGCGGACGCCGCCGATAAATA
CAACGTGCCCACGGTCAAGGTGACGGGCGGCCAGAGGATCGATCTTCTGGGTGTGAAGGGAGAGGATTTGCCCGGCATCT
GGGCGGATCTGAACCGCGCAGGACTGGTCAGCGGGCATGCGTATTCCAAGGGGTTGCGCACGGTGAAGACCTGCGTCGGA
ACGGACCATTGCAGGTTTGGGACGCAGGACAGCACCGGGCTGGGGATCAAGCTGGAGCAGGCGCTGTGGGGGTCGTGGAC
GCCGCACAAGGTGAAGCTTGGAGTCTCGGGTTGTCCGAGGAACTGCGCGGAGGCGACGTGCAAGGATATCGGGATTATCT
GCGTCGACAGCGGCTATCAGGTGAGCGTCGGTGGCGCGGCGGGCATGGACGTGAAGGAAACGGAGCGGTTGTGCGACGTG
GCCACGGAGGCGGAGGCGATTGAAGTTACGATAGCGTTCACGCAGCTGTACCGGGAAAACGCGAAGTACCTGGATCGGGC
CTACAAATGGGTCGCCAAGGTGGGCTTGGACTGGGTCCAAGAGCGCGTGGCCGACTTGGACGAGCGCGCCAGCCTGGTTG
CCGCGTTCGAGTTATCGCAAAGCATTTACCGCAAGGACCCCTGGGCGGAGCATGTCGCGGAGAAGGCCGAGGCGTATCAA
CCGATGGCGGATCTGACGTTGGAGGCCGCGGAATGA

Upstream 100 bases:

>100_bases
ACCGCCAGGATGTCCTCGATTTCCTTGAGGAATACGAGCATGGCGCGAAACCCAGACCAAAAGCCGCAGCGCCCAAAGCT
GTCGCGGCGGAGTGAGCCCC

Downstream 100 bases:

>100_bases
GCTGGGTTGATATCGGAGCGTTGGACGCGGTGCCCGAGCGCGGGGCGCGGTTGGTAAAGACGCAAATGGGCTGCGTGGCG
GTGTTTCGCACCGGCCATGA

Product: nitrite reductase (NAD(P)H) large subunit, NirB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MAKQKLVVIGAGMASGRVLEHLTDAAPDAFDITLFNAEPRGNYNRIMLSPVLAGDQTYADIVTHDEAWYAERAITCRFGE
KVLEIDRDAKIVVGANGHVPYDQLIFGTGSDPFMIPLPGHDLDGVIAYRDLEDTERMMGLQPGQKCVVIGGGLLGLEAAA
GMAARGVDVTVVHIMGHLMERQLDEAAGYLLRKSLEDKGITICLQANSKEIVGRDGQVKALMLDDGTELPCDLLVMAVGI
RPSVALAKTAGLAVGRGIHVDDQMGTSDGAIFAVGECVEHNGAIFGLVAPLYDQARVLAQTLLGEKAAFVQKTLSTKLKV
TGCDLFSAGDFADGETREDIVFRDPARGIYKRLVIEENRLVGAVMYGDTGDGNWFFQLIKDGTDIEEMRETLIFGPSMQG
GPAPDPLSAVAALPRDAEICGCNGVCKGIIVDAIEGGAGDLGAVRGATKASASCGTCTGLVEQLLQVTLGDGFELPSAQP
ICGCCDLTHEDVRRLIKTTPLKSQEAVWQELGWSTRNGCHVCRPAVNFYLLADWPLEYQDDPQSRFINERKHANIQKDGT
FSVVPRMWGGITNPSELRAIADAADKYNVPTVKVTGGQRIDLLGVKGEDLPGIWADLNRAGLVSGHAYSKGLRTVKTCVG
TDHCRFGTQDSTGLGIKLEQALWGSWTPHKVKLGVSGCPRNCAEATCKDIGIICVDSGYQVSVGGAAGMDVKETERLCDV
ATEAEAIEVTIAFTQLYRENAKYLDRAYKWVAKVGLDWVQERVADLDERASLVAAFELSQSIYRKDPWAEHVAEKAEAYQ
PMADLTLEAAE

Sequences:

>Translated_811_residues
MAKQKLVVIGAGMASGRVLEHLTDAAPDAFDITLFNAEPRGNYNRIMLSPVLAGDQTYADIVTHDEAWYAERAITCRFGE
KVLEIDRDAKIVVGANGHVPYDQLIFGTGSDPFMIPLPGHDLDGVIAYRDLEDTERMMGLQPGQKCVVIGGGLLGLEAAA
GMAARGVDVTVVHIMGHLMERQLDEAAGYLLRKSLEDKGITICLQANSKEIVGRDGQVKALMLDDGTELPCDLLVMAVGI
RPSVALAKTAGLAVGRGIHVDDQMGTSDGAIFAVGECVEHNGAIFGLVAPLYDQARVLAQTLLGEKAAFVQKTLSTKLKV
TGCDLFSAGDFADGETREDIVFRDPARGIYKRLVIEENRLVGAVMYGDTGDGNWFFQLIKDGTDIEEMRETLIFGPSMQG
GPAPDPLSAVAALPRDAEICGCNGVCKGIIVDAIEGGAGDLGAVRGATKASASCGTCTGLVEQLLQVTLGDGFELPSAQP
ICGCCDLTHEDVRRLIKTTPLKSQEAVWQELGWSTRNGCHVCRPAVNFYLLADWPLEYQDDPQSRFINERKHANIQKDGT
FSVVPRMWGGITNPSELRAIADAADKYNVPTVKVTGGQRIDLLGVKGEDLPGIWADLNRAGLVSGHAYSKGLRTVKTCVG
TDHCRFGTQDSTGLGIKLEQALWGSWTPHKVKLGVSGCPRNCAEATCKDIGIICVDSGYQVSVGGAAGMDVKETERLCDV
ATEAEAIEVTIAFTQLYRENAKYLDRAYKWVAKVGLDWVQERVADLDERASLVAAFELSQSIYRKDPWAEHVAEKAEAYQ
PMADLTLEAAE
>Mature_810_residues
AKQKLVVIGAGMASGRVLEHLTDAAPDAFDITLFNAEPRGNYNRIMLSPVLAGDQTYADIVTHDEAWYAERAITCRFGEK
VLEIDRDAKIVVGANGHVPYDQLIFGTGSDPFMIPLPGHDLDGVIAYRDLEDTERMMGLQPGQKCVVIGGGLLGLEAAAG
MAARGVDVTVVHIMGHLMERQLDEAAGYLLRKSLEDKGITICLQANSKEIVGRDGQVKALMLDDGTELPCDLLVMAVGIR
PSVALAKTAGLAVGRGIHVDDQMGTSDGAIFAVGECVEHNGAIFGLVAPLYDQARVLAQTLLGEKAAFVQKTLSTKLKVT
GCDLFSAGDFADGETREDIVFRDPARGIYKRLVIEENRLVGAVMYGDTGDGNWFFQLIKDGTDIEEMRETLIFGPSMQGG
PAPDPLSAVAALPRDAEICGCNGVCKGIIVDAIEGGAGDLGAVRGATKASASCGTCTGLVEQLLQVTLGDGFELPSAQPI
CGCCDLTHEDVRRLIKTTPLKSQEAVWQELGWSTRNGCHVCRPAVNFYLLADWPLEYQDDPQSRFINERKHANIQKDGTF
SVVPRMWGGITNPSELRAIADAADKYNVPTVKVTGGQRIDLLGVKGEDLPGIWADLNRAGLVSGHAYSKGLRTVKTCVGT
DHCRFGTQDSTGLGIKLEQALWGSWTPHKVKLGVSGCPRNCAEATCKDIGIICVDSGYQVSVGGAAGMDVKETERLCDVA
TEAEAIEVTIAFTQLYRENAKYLDRAYKWVAKVGLDWVQERVADLDERASLVAAFELSQSIYRKDPWAEHVAEKAEAYQP
MADLTLEAAE

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=312, Percent_Identity=26.2820512820513, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI226437568, Length=312, Percent_Identity=26.6025641025641, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI21389617, Length=312, Percent_Identity=26.6025641025641, Blast_Score=98, Evalue=3e-20,
Organism=Escherichia coli, GI1789765, Length=811, Percent_Identity=36.7447595561036, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789065, Length=369, Percent_Identity=27.9132791327913, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788892, Length=278, Percent_Identity=27.3381294964029, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=408, Percent_Identity=25.7352941176471, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI281359715, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281359713, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24639250, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI18543267, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24639252, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24639257, Length=222, Percent_Identity=29.7297297297297, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585130, Length=290, Percent_Identity=23.1034482758621, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 87296; Mature: 87165

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKLVVIGAGMASGRVLEHLTDAAPDAFDITLFNAEPRGNYNRIMLSPVLAGDQTYAD
CCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCHH
IVTHDEAWYAERAITCRFGEKVLEIDRDAKIVVGANGHVPYDQLIFGTGSDPFMIPLPGH
HEECCCHHHHHHEEEEHHCCHHHHCCCCCEEEEECCCCCCHHHEEEECCCCCEEEECCCC
DLDGVIAYRDLEDTERMMGLQPGQKCVVIGGGLLGLEAAAGMAARGVDVTVVHIMGHLME
CCCCEEEECCCHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCHHCCCCEEHHHHHHHHHH
RQLDEAAGYLLRKSLEDKGITICLQANSKEIVGRDGQVKALMLDDGTELPCDLLVMAVGI
HHHHHHHHHHHHHCCCCCCEEEEEECCCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHCC
RPSVALAKTAGLAVGRGIHVDDQMGTSDGAIFAVGECVEHNGAIFGLVAPLYDQARVLAQ
CCCHHHHHHHCHHCCCCEECCCCCCCCCCCEEEEEHHHHCCCCEEEEHHHHHHHHHHHHH
TLLGEKAAFVQKTLSTKLKVTGCDLFSAGDFADGETREDIVFRDPARGIYKRLVIEENRL
HHHCCHHHHHHHHHHCEEEEEECCEECCCCCCCCCCCCCEEEECCHHHHHHHHHEECCCE
VGAVMYGDTGDGNWFFQLIKDGTDIEEMRETLIFGPSMQGGPAPDPLSAVAALPRDAEIC
EEEEEECCCCCCCEEEEEECCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHCCCCCCEE
GCNGVCKGIIVDAIEGGAGDLGAVRGATKASASCGTCTGLVEQLLQVTLGDGFELPSAQP
CCCCHHCCCEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ICGCCDLTHEDVRRLIKTTPLKSQEAVWQELGWSTRNGCHVCRPAVNFYLLADWPLEYQD
CCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCCCC
DPQSRFINERKHANIQKDGTFSVVPRMWGGITNPSELRAIADAADKYNVPTVKVTGGQRI
CHHHHHHHHHHCCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEE
DLLGVKGEDLPGIWADLNRAGLVSGHAYSKGLRTVKTCVGTDHCRFGTQDSTGLGIKLEQ
EEEECCCCCCCCHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHH
ALWGSWTPHKVKLGVSGCPRNCAEATCKDIGIICVDSGYQVSVGGAAGMDVKETERLCDV
HHCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEECCCCEEEECCCCCCCHHHHHHHHHH
ATEAEAIEVTIAFTQLYRENAKYLDRAYKWVAKVGLDWVQERVADLDERASLVAAFELSQ
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SIYRKDPWAEHVAEKAEAYQPMADLTLEAAE
HHHHCCCHHHHHHHHHHHHCCHHHCEEECCC
>Mature Secondary Structure 
AKQKLVVIGAGMASGRVLEHLTDAAPDAFDITLFNAEPRGNYNRIMLSPVLAGDQTYAD
CCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCHH
IVTHDEAWYAERAITCRFGEKVLEIDRDAKIVVGANGHVPYDQLIFGTGSDPFMIPLPGH
HEECCCHHHHHHEEEEHHCCHHHHCCCCCEEEEECCCCCCHHHEEEECCCCCEEEECCCC
DLDGVIAYRDLEDTERMMGLQPGQKCVVIGGGLLGLEAAAGMAARGVDVTVVHIMGHLME
CCCCEEEECCCHHHHHHHCCCCCCEEEEEECCHHHHHHHCCCHHCCCCEEHHHHHHHHHH
RQLDEAAGYLLRKSLEDKGITICLQANSKEIVGRDGQVKALMLDDGTELPCDLLVMAVGI
HHHHHHHHHHHHHCCCCCCEEEEEECCCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHCC
RPSVALAKTAGLAVGRGIHVDDQMGTSDGAIFAVGECVEHNGAIFGLVAPLYDQARVLAQ
CCCHHHHHHHCHHCCCCEECCCCCCCCCCCEEEEEHHHHCCCCEEEEHHHHHHHHHHHHH
TLLGEKAAFVQKTLSTKLKVTGCDLFSAGDFADGETREDIVFRDPARGIYKRLVIEENRL
HHHCCHHHHHHHHHHCEEEEEECCEECCCCCCCCCCCCCEEEECCHHHHHHHHHEECCCE
VGAVMYGDTGDGNWFFQLIKDGTDIEEMRETLIFGPSMQGGPAPDPLSAVAALPRDAEIC
EEEEEECCCCCCCEEEEEECCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHCCCCCCEE
GCNGVCKGIIVDAIEGGAGDLGAVRGATKASASCGTCTGLVEQLLQVTLGDGFELPSAQP
CCCCHHCCCEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ICGCCDLTHEDVRRLIKTTPLKSQEAVWQELGWSTRNGCHVCRPAVNFYLLADWPLEYQD
CCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCCCC
DPQSRFINERKHANIQKDGTFSVVPRMWGGITNPSELRAIADAADKYNVPTVKVTGGQRI
CHHHHHHHHHHCCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCEE
DLLGVKGEDLPGIWADLNRAGLVSGHAYSKGLRTVKTCVGTDHCRFGTQDSTGLGIKLEQ
EEEECCCCCCCCHHHCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHH
ALWGSWTPHKVKLGVSGCPRNCAEATCKDIGIICVDSGYQVSVGGAAGMDVKETERLCDV
HHCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEECCCCEEEECCCCCCCHHHHHHHHHH
ATEAEAIEVTIAFTQLYRENAKYLDRAYKWVAKVGLDWVQERVADLDERASLVAAFELSQ
HCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
SIYRKDPWAEHVAEKAEAYQPMADLTLEAAE
HHHHCCCHHHHHHHHHHHHCCHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]