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The map label for this gene is fadA [H]

Identifier: 89055466

GI number: 89055466

Start: 2992580

End: 2993791

Strand: Direct

Name: fadA [H]

Synonym: Jann_2975

Alternate gene names: 89055466

Gene position: 2992580-2993791 (Clockwise)

Preceding gene: 89055465

Following gene: 89055467

Centisome position: 69.31

GC content: 63.12

Gene sequence:

>1212_bases
ATGACCGATGCCTTTATCTATGACGCGATCCGCACCCCCCGTGGCAAAGGCCGCCGCGACGGCGCCCTGCATGAGGTGAC
GTCTGCGCGCCTGTCTGCCTTCACCCTCAACACTCTCAAGGAGCGGAATGGCCTTGAAGGTCACGCGGTCGAGGACGTGA
TCTGGGGCAACGTCACCCAGGTGATGGAGCAAGGCGGCTGTCTGGCCCGCGCCGCTGTTCTGGCATCGGACCTGGATGAA
AGCATCCCCGGTCTTGCGATCAACCGCTTCTGCGCCTCCGGCCTTGAGGCCGTGAACCTCGCCGCCAACCAGATCAAAGG
CGGCGCGGGCGATGGCTATATCGCCGGCGGGGTTGAGATGATGGGCCGTGTGGCCATGGGCAGTGACGGTGCCGCCATGG
CCGTGGACCCGCAACTGGCGATGGGCAGCTACTTTGTGCCCCAGGGCATCTCCGCCGATATCATTGCCACGGAATACGGC
TTCACCCGCGATGAGGTGGACGCCTATTCCGTGGAAAGCCAAAACCGTGCTGCGGCGGCCTGGGCCGACCACCGCTTCGG
CAAGTCGGTGATCACGGTCAAAGACCAGAATGGCCTGACGATTCTCGACAAAGACGAATACATGCGGCCCCAGACGGACA
TGCAATCGCTCGGCGCGCTGAAGCCTGCGTTCAAGGACATGGGCGAAGCGATGCCCGGTTTTGACGCCGTGGCGCTGATG
AAGTACCCGCACCTTGAGGCGATCAACCACATCCACCACGCGGGCAACTCCTCGGGTATCGTCGATGGCTCCGCTGCGAT
CCTGGTCGGCAACAAGGAGTTCGGTGAGAAGTGGGGCCTGACGCCCCGCGCAAAGATCCGCGCCAATGCCAAGATCGGCA
CCGATCCCACCATCAACCTGACAGGCCCCGTTCCCGTGACCGAGAAGATCCTGTCGGAAAACGGCATGTCCATCGACGAC
ATCGGTCTGTTTGAGGTGAATGAGGCGTTTGCCGCCGTCGTGCTGCGCTTCCTGCAAGCGTTTGACGTGGACCATGCGAA
GGTCAACGTGAATGGCGGTGCCATCGCCATGGGCCACCCCCTGGGGGCCACGGGGGCGATGATCCTTGGCACCCTGCTGG
ACGAGATGGAACGTGCGGATAAGGAAACCGGCCTCGCCACGCTCTGTGTGGCCGCTGGCATGGGATCTGCCACGATCCTG
GAGCGGGTGTAG

Upstream 100 bases:

>100_bases
AGGCCGGGATTGCGCCTGCGCCCACCGGCTTCCCATATTTGACACATACCCGGCCCGCCCCTTGCCTGAAGCGGCGCGGC
CAGAGCTTTGGAGACGACCC

Downstream 100 bases:

>100_bases
CCCCGCCGCCTCGGCACGCCCCGCCCCCCGCACGGCAAGAGAAAGGAGGACCGCATGACACATCACGACGATACGGCGAC
GGCGGCGCGATCTGCGCAAG

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTDAFIYDAIRTPRGKGRRDGALHEVTSARLSAFTLNTLKERNGLEGHAVEDVIWGNVTQVMEQGGCLARAAVLASDLDE
SIPGLAINRFCASGLEAVNLAANQIKGGAGDGYIAGGVEMMGRVAMGSDGAAMAVDPQLAMGSYFVPQGISADIIATEYG
FTRDEVDAYSVESQNRAAAAWADHRFGKSVITVKDQNGLTILDKDEYMRPQTDMQSLGALKPAFKDMGEAMPGFDAVALM
KYPHLEAINHIHHAGNSSGIVDGSAAILVGNKEFGEKWGLTPRAKIRANAKIGTDPTINLTGPVPVTEKILSENGMSIDD
IGLFEVNEAFAAVVLRFLQAFDVDHAKVNVNGGAIAMGHPLGATGAMILGTLLDEMERADKETGLATLCVAAGMGSATIL
ERV

Sequences:

>Translated_403_residues
MTDAFIYDAIRTPRGKGRRDGALHEVTSARLSAFTLNTLKERNGLEGHAVEDVIWGNVTQVMEQGGCLARAAVLASDLDE
SIPGLAINRFCASGLEAVNLAANQIKGGAGDGYIAGGVEMMGRVAMGSDGAAMAVDPQLAMGSYFVPQGISADIIATEYG
FTRDEVDAYSVESQNRAAAAWADHRFGKSVITVKDQNGLTILDKDEYMRPQTDMQSLGALKPAFKDMGEAMPGFDAVALM
KYPHLEAINHIHHAGNSSGIVDGSAAILVGNKEFGEKWGLTPRAKIRANAKIGTDPTINLTGPVPVTEKILSENGMSIDD
IGLFEVNEAFAAVVLRFLQAFDVDHAKVNVNGGAIAMGHPLGATGAMILGTLLDEMERADKETGLATLCVAAGMGSATIL
ERV
>Mature_402_residues
TDAFIYDAIRTPRGKGRRDGALHEVTSARLSAFTLNTLKERNGLEGHAVEDVIWGNVTQVMEQGGCLARAAVLASDLDES
IPGLAINRFCASGLEAVNLAANQIKGGAGDGYIAGGVEMMGRVAMGSDGAAMAVDPQLAMGSYFVPQGISADIIATEYGF
TRDEVDAYSVESQNRAAAAWADHRFGKSVITVKDQNGLTILDKDEYMRPQTDMQSLGALKPAFKDMGEAMPGFDAVALMK
YPHLEAINHIHHAGNSSGIVDGSAAILVGNKEFGEKWGLTPRAKIRANAKIGTDPTINLTGPVPVTEKILSENGMSIDDI
GLFEVNEAFAAVVLRFLQAFDVDHAKVNVNGGAIAMGHPLGATGAMILGTLLDEMERADKETGLATLCVAAGMGSATILE
RV

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=409, Percent_Identity=33.0073349633252, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI167614485, Length=411, Percent_Identity=31.1435523114355, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI148539872, Length=404, Percent_Identity=31.6831683168317, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4557237, Length=417, Percent_Identity=30.4556354916067, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI4504327, Length=449, Percent_Identity=26.5033407572383, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI194353979, Length=239, Percent_Identity=30.1255230125523, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI48994986, Length=411, Percent_Identity=38.4428223844282, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1787663, Length=427, Percent_Identity=36.768149882904, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1788554, Length=418, Percent_Identity=35.8851674641148, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87082165, Length=418, Percent_Identity=35.6459330143541, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788683, Length=443, Percent_Identity=27.313769751693, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI133906874, Length=420, Percent_Identity=32.1428571428571, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI25147385, Length=418, Percent_Identity=29.9043062200957, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535921, Length=420, Percent_Identity=28.5714285714286, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17551802, Length=459, Percent_Identity=25.0544662309368, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17535917, Length=406, Percent_Identity=27.0935960591133, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322031, Length=407, Percent_Identity=32.4324324324324, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6325229, Length=422, Percent_Identity=28.6729857819905, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24655093, Length=416, Percent_Identity=35.3365384615385, Blast_Score=215, Evalue=5e-56,
Organism=Drosophila melanogaster, GI17648125, Length=414, Percent_Identity=31.6425120772947, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24640423, Length=417, Percent_Identity=30.4556354916067, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137578, Length=447, Percent_Identity=26.1744966442953, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42480; Mature: 42349

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAFIYDAIRTPRGKGRRDGALHEVTSARLSAFTLNTLKERNGLEGHAVEDVIWGNVTQ
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHH
VMEQGGCLARAAVLASDLDESIPGLAINRFCASGLEAVNLAANQIKGGAGDGYIAGGVEM
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCHHH
MGRVAMGSDGAAMAVDPQLAMGSYFVPQGISADIIATEYGFTRDEVDAYSVESQNRAAAA
HHHHEECCCCCEEEECCCHHCCCEECCCCCCCCEEEECCCCCHHCCCCEECCCCCCHHHH
WADHRFGKSVITVKDQNGLTILDKDEYMRPQTDMQSLGALKPAFKDMGEAMPGFDAVALM
HHHHHCCCEEEEEECCCCCEEEECHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHH
KYPHLEAINHIHHAGNSSGIVDGSAAILVGNKEFGEKWGLTPRAKIRANAKIGTDPTINL
HCCCHHHHHHHHHCCCCCCEECCCEEEEECCHHHHHHCCCCCCCEEEECCCCCCCCEEEE
TGPVPVTEKILSENGMSIDDIGLFEVNEAFAAVVLRFLQAFDVDHAKVNVNGGAIAMGHP
CCCCCHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCC
LGATGAMILGTLLDEMERADKETGLATLCVAAGMGSATILERV
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure 
TDAFIYDAIRTPRGKGRRDGALHEVTSARLSAFTLNTLKERNGLEGHAVEDVIWGNVTQ
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHH
VMEQGGCLARAAVLASDLDESIPGLAINRFCASGLEAVNLAANQIKGGAGDGYIAGGVEM
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCHHH
MGRVAMGSDGAAMAVDPQLAMGSYFVPQGISADIIATEYGFTRDEVDAYSVESQNRAAAA
HHHHEECCCCCEEEECCCHHCCCEECCCCCCCCEEEECCCCCHHCCCCEECCCCCCHHHH
WADHRFGKSVITVKDQNGLTILDKDEYMRPQTDMQSLGALKPAFKDMGEAMPGFDAVALM
HHHHHCCCEEEEEECCCCCEEEECHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHH
KYPHLEAINHIHHAGNSSGIVDGSAAILVGNKEFGEKWGLTPRAKIRANAKIGTDPTINL
HCCCHHHHHHHHHCCCCCCEECCCEEEEECCHHHHHHCCCCCCCEEEECCCCCCCCEEEE
TGPVPVTEKILSENGMSIDDIGLFEVNEAFAAVVLRFLQAFDVDHAKVNVNGGAIAMGHP
CCCCCHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCC
LGATGAMILGTLLDEMERADKETGLATLCVAAGMGSATILERV
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]