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The map label for this gene is cobQ

Identifier: 89055428

GI number: 89055428

Start: 2959311

End: 2960756

Strand: Direct

Name: cobQ

Synonym: Jann_2937

Alternate gene names: 89055428

Gene position: 2959311-2960756 (Clockwise)

Preceding gene: 89055427

Following gene: 89055431

Centisome position: 68.53

GC content: 63.28

Gene sequence:

>1446_bases
ATGTCCCGCGCGCTGATGATCCAGGGCACCGGCTCCAACGTGGGCAAGTCGATGCTTGCCGCGGGCCTGTGCCGCATCGC
GCGCAATCGGGGGCTGTCGGTCGCGCCTTTCAAGCCGCAGAACATGTCCAACAACGCCGCTGTGACGGCGGATGGCGGGG
AAATCGGGCGTGCGCAGGCGTTGCAAGCCATGGCGTGCGGGCTGGAGCCCCACACGGATATGAACCCTGTCTTGCTAAAG
CCGGAAACAGAGACGGGATCTCAGGTCGTCGTTCAGGGCAAACGGTTTACCACGGTGCGGGCGCGCGACTATGCCAAGCT
GAAACCGCAATTGATGCAGGCTGTGCTGGACAGTTTTAAGCGGCTGAAAGCGGCCCATGATCTGGTGATCGTTGAAGGCG
CTGGATCTCCGGCGGAAGTGAATTTGCGAAATGGAGACATCGCGAATATGGGGTTTGCGCAGGCGTCCGGCACGCCGGTG
GTGCTGTGCGGGGACATCGACCGGGGCGGCGTGATCGCGCAGATCGTCGGCACGCAAGCGGTCATGTCTGCCGAGGATGT
AGCGCTTGTACGCGGCTTCATGATCAACAAGTTTCGCGGCGATCCATCGCTATTCGACGATGGCTACAAACTCATTGAAC
AGCATACGGGATGGCAGGGTTTCGGGGTCATTCCTTGGTTTGCAGATGCGGGCAATTTGCCTGCGGAAGACGCGCTGGAC
ATCACCACGCGCACCCGTGACACGGGCCTGCACATCGTCTGCCTGCGCCTGTCACGGATCGCGAATTTCGACGACATGGA
CCCGCTGGCCCAGGAACCCGGCGTGCGATTGACCATGCTGAACGCGGGGGAGGCCATACCCGGTGACGCCGATATGGTGA
TCATTCCGGGCAGCAAATCCACGCGGGGTGATCTGGCGTACCTGCGCGCTCAGGGTTGGGATCTGGATCTGCGCGCGCAC
TTGCGACGCGGCGGTCACGTTTTGGGAATTTGCGGCGGATATCAGATGCTTGGTGGCTCCGTCGCTGATCCTGAAGGCGT
TGAAGGCCCGGCGGGCACGGACGAGGGCCTTGGACTGCTGGACGTGGAGACGGTCATGACCGGTGACAAGCGGCTGACGC
GGGTGGCCGCAATCCACGCGCCCTCGGGCACCGCGTTCAACGGCTATGAGATCCACATAGGGCGCACGTCTGGCCCGGAT
GCGGCCCGTCCCTTCGCGGTGGTGAATGGCCAGCCTGAGGGCGCGATCAGTGGCGACGGGCGCGTGTCGGGCAGCTATCT
TCACGGGATGTTCCGCGACGACGCTTTCCGCGCGGCCTGGCTGGCACAATTTGGCGTGGCGCAAAGCGTCAGCTACGACG
CAACCGTGCAGGCGACGTTGGACGCTTTGGCGGCGCATCTGGAGGGCGTGATGGACATCGACGCCCTGCTGGATGTGCGC
CTATAG

Upstream 100 bases:

>100_bases
CTGCAACGGGCGAGACGGTCACGATCCGCGATGGCGAGAAGGTCTACCGGGATGCGGACGGGGTCGAGACGGCGCGCAAG
CCGTATCACGGGGCCTGAGA

Downstream 100 bases:

>100_bases
ACCTAAAAAATCGGTGATCGCTGCATTGAAAGCCGCTGGCGCGTTGATGTTGGCGACATGGGCCGCGCCTTCGATCTGGC
GATAGTCACCGCCCGGCGTC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSRALMIQGTGSNVGKSMLAAGLCRIARNRGLSVAPFKPQNMSNNAAVTADGGEIGRAQALQAMACGLEPHTDMNPVLLK
PETETGSQVVVQGKRFTTVRARDYAKLKPQLMQAVLDSFKRLKAAHDLVIVEGAGSPAEVNLRNGDIANMGFAQASGTPV
VLCGDIDRGGVIAQIVGTQAVMSAEDVALVRGFMINKFRGDPSLFDDGYKLIEQHTGWQGFGVIPWFADAGNLPAEDALD
ITTRTRDTGLHIVCLRLSRIANFDDMDPLAQEPGVRLTMLNAGEAIPGDADMVIIPGSKSTRGDLAYLRAQGWDLDLRAH
LRRGGHVLGICGGYQMLGGSVADPEGVEGPAGTDEGLGLLDVETVMTGDKRLTRVAAIHAPSGTAFNGYEIHIGRTSGPD
AARPFAVVNGQPEGAISGDGRVSGSYLHGMFRDDAFRAAWLAQFGVAQSVSYDATVQATLDALAAHLEGVMDIDALLDVR
L

Sequences:

>Translated_481_residues
MSRALMIQGTGSNVGKSMLAAGLCRIARNRGLSVAPFKPQNMSNNAAVTADGGEIGRAQALQAMACGLEPHTDMNPVLLK
PETETGSQVVVQGKRFTTVRARDYAKLKPQLMQAVLDSFKRLKAAHDLVIVEGAGSPAEVNLRNGDIANMGFAQASGTPV
VLCGDIDRGGVIAQIVGTQAVMSAEDVALVRGFMINKFRGDPSLFDDGYKLIEQHTGWQGFGVIPWFADAGNLPAEDALD
ITTRTRDTGLHIVCLRLSRIANFDDMDPLAQEPGVRLTMLNAGEAIPGDADMVIIPGSKSTRGDLAYLRAQGWDLDLRAH
LRRGGHVLGICGGYQMLGGSVADPEGVEGPAGTDEGLGLLDVETVMTGDKRLTRVAAIHAPSGTAFNGYEIHIGRTSGPD
AARPFAVVNGQPEGAISGDGRVSGSYLHGMFRDDAFRAAWLAQFGVAQSVSYDATVQATLDALAAHLEGVMDIDALLDVR
L
>Mature_480_residues
SRALMIQGTGSNVGKSMLAAGLCRIARNRGLSVAPFKPQNMSNNAAVTADGGEIGRAQALQAMACGLEPHTDMNPVLLKP
ETETGSQVVVQGKRFTTVRARDYAKLKPQLMQAVLDSFKRLKAAHDLVIVEGAGSPAEVNLRNGDIANMGFAQASGTPVV
LCGDIDRGGVIAQIVGTQAVMSAEDVALVRGFMINKFRGDPSLFDDGYKLIEQHTGWQGFGVIPWFADAGNLPAEDALDI
TTRTRDTGLHIVCLRLSRIANFDDMDPLAQEPGVRLTMLNAGEAIPGDADMVIIPGSKSTRGDLAYLRAQGWDLDLRAHL
RRGGHVLGICGGYQMLGGSVADPEGVEGPAGTDEGLGLLDVETVMTGDKRLTRVAAIHAPSGTAFNGYEIHIGRTSGPDA
ARPFAVVNGQPEGAISGDGRVSGSYLHGMFRDDAFRAAWLAQFGVAQSVSYDATVQATLDALAAHLEGVMDIDALLDVRL

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_JANSC (Q28N58)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510879.1
- ProteinModelPortal:   Q28N58
- STRING:   Q28N58
- GeneID:   3935407
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2937
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- BioCyc:   JSP290400:JANN_2937-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 50771; Mature: 50640

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 331-331 ACT_SITE 428-428

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRALMIQGTGSNVGKSMLAAGLCRIARNRGLSVAPFKPQNMSNNAAVTADGGEIGRAQA
CCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCCHHHH
LQAMACGLEPHTDMNPVLLKPETETGSQVVVQGKRFTTVRARDYAKLKPQLMQAVLDSFK
HHHHHCCCCCCCCCCCEEEECCCCCCCEEEEECCEEEEEECCCHHHHCHHHHHHHHHHHH
RLKAAHDLVIVEGAGSPAEVNLRNGDIANMGFAQASGTPVVLCGDIDRGGVIAQIVGTQA
HHHHHCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCHH
VMSAEDVALVRGFMINKFRGDPSLFDDGYKLIEQHTGWQGFGVIPWFADAGNLPAEDALD
HHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEE
ITTRTRDTGLHIVCLRLSRIANFDDMDPLAQEPGVRLTMLNAGEAIPGDADMVIIPGSKS
EEECCCCCCEEEEEEEHHHHCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCC
TRGDLAYLRAQGWDLDLRAHLRRGGHVLGICGGYQMLGGSVADPEGVEGPAGTDEGLGLL
CCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCEEE
DVETVMTGDKRLTRVAAIHAPSGTAFNGYEIHIGRTSGPDAARPFAVVNGQPEGAISGDG
EEHHHHCCCHHEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
RVSGSYLHGMFRDDAFRAAWLAQFGVAQSVSYDATVQATLDALAAHLEGVMDIDALLDVR
EECHHHHHHHHHCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHEEC
L
C
>Mature Secondary Structure 
SRALMIQGTGSNVGKSMLAAGLCRIARNRGLSVAPFKPQNMSNNAAVTADGGEIGRAQA
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCCHHHH
LQAMACGLEPHTDMNPVLLKPETETGSQVVVQGKRFTTVRARDYAKLKPQLMQAVLDSFK
HHHHHCCCCCCCCCCCEEEECCCCCCCEEEEECCEEEEEECCCHHHHCHHHHHHHHHHHH
RLKAAHDLVIVEGAGSPAEVNLRNGDIANMGFAQASGTPVVLCGDIDRGGVIAQIVGTQA
HHHHHCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCHH
VMSAEDVALVRGFMINKFRGDPSLFDDGYKLIEQHTGWQGFGVIPWFADAGNLPAEDALD
HHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEE
ITTRTRDTGLHIVCLRLSRIANFDDMDPLAQEPGVRLTMLNAGEAIPGDADMVIIPGSKS
EEECCCCCCEEEEEEEHHHHCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCC
TRGDLAYLRAQGWDLDLRAHLRRGGHVLGICGGYQMLGGSVADPEGVEGPAGTDEGLGLL
CCCCEEEEEECCCCCHHHHHHHCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCEEE
DVETVMTGDKRLTRVAAIHAPSGTAFNGYEIHIGRTSGPDAARPFAVVNGQPEGAISGDG
EEHHHHCCCHHEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
RVSGSYLHGMFRDDAFRAAWLAQFGVAQSVSYDATVQATLDALAAHLEGVMDIDALLDVR
EECHHHHHHHHHCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHEEC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA