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The map label for this gene is ageIM [H]

Identifier: 89055380

GI number: 89055380

Start: 2909075

End: 2910196

Strand: Direct

Name: ageIM [H]

Synonym: Jann_2889

Alternate gene names: 89055380

Gene position: 2909075-2910196 (Clockwise)

Preceding gene: 89055379

Following gene: 89055381

Centisome position: 67.37

GC content: 65.15

Gene sequence:

>1122_bases
ATGACATACACAGCGATAGACTTGTTTTGCGGGGCTGGCGGCCTGTCCGCCGGGCTTGAGATGGCAGGCTTCACCGTGCT
GGCGGGCAATGACCTCTTTGACGCGGCGGGCCGGACGTTTGAGGCCACGCACCCGAGGGCGAAGTTTATCTCCGGCCCGA
TTGAGGAGCTTTCTGTTGAGCGCCTGATGGAAGTCACGGGCCTGCGCAAGGGCGAGTTGTCGGTGCTGGTCGGTGGCCCG
CCCTGTCAGGCCTATTCGGTCTACAACCACCAGCGCGGGATGCACGACGCCCGGGCTTCCCTGTTCCGGGAATACCTGCG
GATAGTCGATGGCCTGCGGCCCGAATGGATTGTGATGGAGAACGTCACCGGCATCTACTCCATCGCCAACGGGGAAGCCG
TCCGGGCGATCAAGGCGGAGTTTGCGGCCCTCGGTTATGCCGTGGAGGACGCGGTGCTGCGCGCAGAGGACTACGGGGTG
CCACAGGAACGGCGGCGCGTCGTGTTCATTGGCAACCGCGTAGGTGCGCCCATCTCGCACCCGGAGCCCACGCACGGGCC
GGGGCGCGCTAACCCGTTCACCAAGATCAGGGACGCAATAGGGGACCTGCCCCCGCTCGACAATGGCGAGAACCCGGGCC
GCGTTGCCTACGCTGATGCCAGACCGAGCAAATACCAGCGCCAGATGCGCGGGAACGCGATCACCGTGGGCAACCACGCA
GCCCCGAGGCTCGGCAAGGTCAACATGGACCGGCTGCCCTACATCCCACCCGGCGGGAGCTGGCGCGACATTCCGCATGA
CCTGCTGCCCGAGGGGATGAAGCGCGCCAAGCGGTCGGACCATACCAAGCGATACGGGCGCATGACATGGGACGGGCTTT
CTTGCACAGTCCTGACCAAATGCGACATCCACTGGGGGGCCTACATCCACCCGGAACAGGACCGCGCCATTTCGGTGCGC
GAGGCGGCGCGGCTCCAAGCCTTCCCCGACTGGTTCGAGTTTGCTGGATCGAAGACAGAACAGTATGTGCAGGTCGGCAA
CGCGGTCCCGCCCCTCTTGGGCAAGGCTGTTGGCGACCACCTGATGCAGCTCATGGCTGCGCAGGGCGAAGTCGCAGCCT
GA

Upstream 100 bases:

>100_bases
GGCGCGGATCACGGCGAAGGCCCAGAAATAGAAGTTGCCCAGTTAACCCCCTGTAAGGTAAGTCTGGGACAAAATGAGAA
CAAAAGCGGAGGCGAGCCCG

Downstream 100 bases:

>100_bases
CACCGGCCCGTCCGTCGCACTAATTCCCGCCGATTCAGCGTCCAATTGGCGCGACCGACACAGGGCGAGCCTTGTGAGAC
TTCTCCAACCACTGGCCGAT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.AgeI; Cytosine-specific methyltransferase AgeI [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTYTAIDLFCGAGGLSAGLEMAGFTVLAGNDLFDAAGRTFEATHPRAKFISGPIEELSVERLMEVTGLRKGELSVLVGGP
PCQAYSVYNHQRGMHDARASLFREYLRIVDGLRPEWIVMENVTGIYSIANGEAVRAIKAEFAALGYAVEDAVLRAEDYGV
PQERRRVVFIGNRVGAPISHPEPTHGPGRANPFTKIRDAIGDLPPLDNGENPGRVAYADARPSKYQRQMRGNAITVGNHA
APRLGKVNMDRLPYIPPGGSWRDIPHDLLPEGMKRAKRSDHTKRYGRMTWDGLSCTVLTKCDIHWGAYIHPEQDRAISVR
EAARLQAFPDWFEFAGSKTEQYVQVGNAVPPLLGKAVGDHLMQLMAAQGEVAA

Sequences:

>Translated_373_residues
MTYTAIDLFCGAGGLSAGLEMAGFTVLAGNDLFDAAGRTFEATHPRAKFISGPIEELSVERLMEVTGLRKGELSVLVGGP
PCQAYSVYNHQRGMHDARASLFREYLRIVDGLRPEWIVMENVTGIYSIANGEAVRAIKAEFAALGYAVEDAVLRAEDYGV
PQERRRVVFIGNRVGAPISHPEPTHGPGRANPFTKIRDAIGDLPPLDNGENPGRVAYADARPSKYQRQMRGNAITVGNHA
APRLGKVNMDRLPYIPPGGSWRDIPHDLLPEGMKRAKRSDHTKRYGRMTWDGLSCTVLTKCDIHWGAYIHPEQDRAISVR
EAARLQAFPDWFEFAGSKTEQYVQVGNAVPPLLGKAVGDHLMQLMAAQGEVAA
>Mature_372_residues
TYTAIDLFCGAGGLSAGLEMAGFTVLAGNDLFDAAGRTFEATHPRAKFISGPIEELSVERLMEVTGLRKGELSVLVGGPP
CQAYSVYNHQRGMHDARASLFREYLRIVDGLRPEWIVMENVTGIYSIANGEAVRAIKAEFAALGYAVEDAVLRAEDYGVP
QERRRVVFIGNRVGAPISHPEPTHGPGRANPFTKIRDAIGDLPPLDNGENPGRVAYADARPSKYQRQMRGNAITVGNHAA
PRLGKVNMDRLPYIPPGGSWRDIPHDLLPEGMKRAKRSDHTKRYGRMTWDGLSCTVLTKCDIHWGAYIHPEQDRAISVRE
AARLQAFPDWFEFAGSKTEQYVQVGNAVPPLLGKAVGDHLMQLMAAQGEVAA

Specific function: This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the AgeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=468, Percent_Identity=28.2051282051282, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI4503351, Length=468, Percent_Identity=28.2051282051282, Blast_Score=162, Evalue=4e-40,
Organism=Escherichia coli, GI1788271, Length=199, Percent_Identity=29.6482412060301, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40859; Mature: 40728

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYTAIDLFCGAGGLSAGLEMAGFTVLAGNDLFDAAGRTFEATHPRAKFISGPIEELSVE
CCEEEEEEECCCCCHHHCHHHHCEEEEECCCHHHHCCCEEECCCCHHHHHCCCHHHHHHH
RLMEVTGLRKGELSVLVGGPPCQAYSVYNHQRGMHDARASLFREYLRIVDGLRPEWIVME
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
NVTGIYSIANGEAVRAIKAEFAALGYAVEDAVLRAEDYGVPQERRRVVFIGNRVGAPISH
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCCCCC
PEPTHGPGRANPFTKIRDAIGDLPPLDNGENPGRVAYADARPSKYQRQMRGNAITVGNHA
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCEEEECCCC
APRLGKVNMDRLPYIPPGGSWRDIPHDLLPEGMKRAKRSDHTKRYGRMTWDGLSCTVLTK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEE
CDIHWGAYIHPEQDRAISVREAARLQAFPDWFEFAGSKTEQYVQVGNAVPPLLGKAVGDH
ECCCCCCEECCCCCCEEEHHHHHHHHCCHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
LMQLMAAQGEVAA
HHHHHHHCCCCCC
>Mature Secondary Structure 
TYTAIDLFCGAGGLSAGLEMAGFTVLAGNDLFDAAGRTFEATHPRAKFISGPIEELSVE
CEEEEEEECCCCCHHHCHHHHCEEEEECCCHHHHCCCEEECCCCHHHHHCCCHHHHHHH
RLMEVTGLRKGELSVLVGGPPCQAYSVYNHQRGMHDARASLFREYLRIVDGLRPEWIVME
HHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
NVTGIYSIANGEAVRAIKAEFAALGYAVEDAVLRAEDYGVPQERRRVVFIGNRVGAPISH
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCCCCC
PEPTHGPGRANPFTKIRDAIGDLPPLDNGENPGRVAYADARPSKYQRQMRGNAITVGNHA
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCEEEECCCC
APRLGKVNMDRLPYIPPGGSWRDIPHDLLPEGMKRAKRSDHTKRYGRMTWDGLSCTVLTK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEE
CDIHWGAYIHPEQDRAISVREAARLQAFPDWFEFAGSKTEQYVQVGNAVPPLLGKAVGDH
ECCCCCCEECCCCCCEEEHHHHHHHHCCHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHH
LMQLMAAQGEVAA
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8901102 [H]