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The map label for this gene is ddeIM [H]

Identifier: 89055349

GI number: 89055349

Start: 2877976

End: 2879484

Strand: Direct

Name: ddeIM [H]

Synonym: Jann_2858

Alternate gene names: 89055349

Gene position: 2877976-2879484 (Clockwise)

Preceding gene: 89055347

Following gene: 89055350

Centisome position: 66.65

GC content: 59.71

Gene sequence:

>1509_bases
ATGGACGGAAGCTTTGGCATTATCGACCTCTTCGCTGGCCCAGGCGGCCTTGCGGAGGGGTTCTCCGCAGCAGGCCGCGA
GACGGATACACGCATGAAGATCCGCCTCTCCATCGAGAAGGAGGCGACGGAGGTGCGCACACTTCGGCTGCGCGCATTCC
TGCGTGGGTTCGATGGTGGCTTCCCGACGGAATACTACGCGGCACTCAACGCTAGCGAACCGCTACCTGACTGGTCGGAG
CTTTTTCCCGCCCGCTGGGCTGACGCCTGCAAGGAGGCGTGGCAAATGGAGTTGGGGCAGCCCGGTGTGTTCGAGGTGAT
CGCGAACGTCCTCGATCGGACGCGCGAGGAACATGATGGTAACACCATCCTCATCGGCGGCCCCCCGTGCCAAGCCTACA
GTCTGGCTGGACGTGCGAGAAACAAAGGTGTTGCGGACTACGTGGCAGAGAAGGATTCGAGGCATTTCCTCTACAGAGAG
TACGTAGAGATTCTGAAGCGGCTAAGGCCCGCCGTCTTCGTCATGGAGAACGTCAAGGGCATGCTGTCCAGCAAGGTGAA
TGGCGGCGAGATCTTCGAGCGCGTCCTTGACGACCTACGCAACGCAGGTGACGGCTATACGCTCGTGCCTTTGTCTGCTC
AGCGCAACCGTGAACATTTAGCGGCCCGGGACTTCATTGTACGCGCCGAGGAACACGGTGTCCCGCAGGCGAGGCATCGA
GTGTTCGTCGTCGGCATACGCAATGATCTGCCGACGCCGGAAGGCGACATGCCCCTCATGCCAGCCGTAGATCAGAACTG
CCGCGCGGATGTACATTCCGTTCTGGGCAGCCTGCCTGCACTTCGCAGCGGCCTGAGCCGTCAGCGAGACAATCCCGCAG
CTTGGCGCAAAGTCGTACTTGAACACGCCAAACGTCTCATCGCCAGCAAGAAGGTGGATTCCGAACTGCGCCAGTATCTT
GCAGAGCTGAAGAAAAGCAGTTTGGCGCGTGCATTCGAGCGTTGCCAGTCGACGAGGTCGTGTGATGAGAGCGGGATGCC
TCCCCAGCTTGCCGAATGGTTGATCGATCCCGCTCTGCAGCGGATCAGCGGACACGAGACCCGTGCGCATATCGCCGCGG
ATCTAGGCCGATATCTTTTCGTCTCGGCCTTTGGACGTGAGTACGGCCGATCGCCCAAGCTCTCCGAATTTCCCGACTAT
CTGCTACCTGCTCACCGAAATCGCTATAGCGGAGCCTTCGCTGACAGGTTCCGGGTGCAGATAGCCGAACGTCCCTCCAG
CACGGTCACTAGCCACATCTCGAAGGATGGCCACTATTTCATCCATCCGGATCCGACTCAGGTCCGTTCACTAACAGTTC
GCGAGGCCGCGCGCCTCCAGACCTTCCCGGACAACTACGTCTTTTGCGGAAACAGGACACAGCAATATCATCAGGTCGGA
AACGCGGTTCCTCCGTTCCTAGCGCTACGGATCGCACGTGTGATCCGAGACATTATGGGGCGCTTCTGA

Upstream 100 bases:

>100_bases
TTTCCGCCGAAACGATGTCTACCATCGATGAGTGCCCCCTTTGCCCCTGGCACGGCCTGCAGTCTTCAGTTTAGAAGGTT
GCTCGAGAAAGGTGAAATGA

Downstream 100 bases:

>100_bases
TCCGGCTTTCCGGCAGTATCTCCACGTTGCGAAATGCCCTCCGAAGACCTGGCGAAGGCATGTTCCACTTCCTTGGCGAG
TTCCTCAGGGGAAAGGTAGG

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MDGSFGIIDLFAGPGGLAEGFSAAGRETDTRMKIRLSIEKEATEVRTLRLRAFLRGFDGGFPTEYYAALNASEPLPDWSE
LFPARWADACKEAWQMELGQPGVFEVIANVLDRTREEHDGNTILIGGPPCQAYSLAGRARNKGVADYVAEKDSRHFLYRE
YVEILKRLRPAVFVMENVKGMLSSKVNGGEIFERVLDDLRNAGDGYTLVPLSAQRNREHLAARDFIVRAEEHGVPQARHR
VFVVGIRNDLPTPEGDMPLMPAVDQNCRADVHSVLGSLPALRSGLSRQRDNPAAWRKVVLEHAKRLIASKKVDSELRQYL
AELKKSSLARAFERCQSTRSCDESGMPPQLAEWLIDPALQRISGHETRAHIAADLGRYLFVSAFGREYGRSPKLSEFPDY
LLPAHRNRYSGAFADRFRVQIAERPSSTVTSHISKDGHYFIHPDPTQVRSLTVREAARLQTFPDNYVFCGNRTQQYHQVG
NAVPPFLALRIARVIRDIMGRF

Sequences:

>Translated_502_residues
MDGSFGIIDLFAGPGGLAEGFSAAGRETDTRMKIRLSIEKEATEVRTLRLRAFLRGFDGGFPTEYYAALNASEPLPDWSE
LFPARWADACKEAWQMELGQPGVFEVIANVLDRTREEHDGNTILIGGPPCQAYSLAGRARNKGVADYVAEKDSRHFLYRE
YVEILKRLRPAVFVMENVKGMLSSKVNGGEIFERVLDDLRNAGDGYTLVPLSAQRNREHLAARDFIVRAEEHGVPQARHR
VFVVGIRNDLPTPEGDMPLMPAVDQNCRADVHSVLGSLPALRSGLSRQRDNPAAWRKVVLEHAKRLIASKKVDSELRQYL
AELKKSSLARAFERCQSTRSCDESGMPPQLAEWLIDPALQRISGHETRAHIAADLGRYLFVSAFGREYGRSPKLSEFPDY
LLPAHRNRYSGAFADRFRVQIAERPSSTVTSHISKDGHYFIHPDPTQVRSLTVREAARLQTFPDNYVFCGNRTQQYHQVG
NAVPPFLALRIARVIRDIMGRF
>Mature_502_residues
MDGSFGIIDLFAGPGGLAEGFSAAGRETDTRMKIRLSIEKEATEVRTLRLRAFLRGFDGGFPTEYYAALNASEPLPDWSE
LFPARWADACKEAWQMELGQPGVFEVIANVLDRTREEHDGNTILIGGPPCQAYSLAGRARNKGVADYVAEKDSRHFLYRE
YVEILKRLRPAVFVMENVKGMLSSKVNGGEIFERVLDDLRNAGDGYTLVPLSAQRNREHLAARDFIVRAEEHGVPQARHR
VFVVGIRNDLPTPEGDMPLMPAVDQNCRADVHSVLGSLPALRSGLSRQRDNPAAWRKVVLEHAKRLIASKKVDSELRQYL
AELKKSSLARAFERCQSTRSCDESGMPPQLAEWLIDPALQRISGHETRAHIAADLGRYLFVSAFGREYGRSPKLSEFPDY
LLPAHRNRYSGAFADRFRVQIAERPSSTVTSHISKDGHYFIHPDPTQVRSLTVREAARLQTFPDNYVFCGNRTQQYHQVG
NAVPPFLALRIARVIRDIMGRF

Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 56419; Mature: 56419

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGSFGIIDLFAGPGGLAEGFSAAGRETDTRMKIRLSIEKEATEVRTLRLRAFLRGFDGG
CCCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
FPTEYYAALNASEPLPDWSELFPARWADACKEAWQMELGQPGVFEVIANVLDRTREEHDG
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
NTILIGGPPCQAYSLAGRARNKGVADYVAEKDSRHFLYREYVEILKRLRPAVFVMENVKG
CEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLSSKVNGGEIFERVLDDLRNAGDGYTLVPLSAQRNREHLAARDFIVRAEEHGVPQARHR
HHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHCE
VFVVGIRNDLPTPEGDMPLMPAVDQNCRADVHSVLGSLPALRSGLSRQRDNPAAWRKVVL
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EHAKRLIASKKVDSELRQYLAELKKSSLARAFERCQSTRSCDESGMPPQLAEWLIDPALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RISGHETRAHIAADLGRYLFVSAFGREYGRSPKLSEFPDYLLPAHRNRYSGAFADRFRVQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
IAERPSSTVTSHISKDGHYFIHPDPTQVRSLTVREAARLQTFPDNYVFCGNRTQQYHQVG
HHCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHC
NAVPPFLALRIARVIRDIMGRF
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDGSFGIIDLFAGPGGLAEGFSAAGRETDTRMKIRLSIEKEATEVRTLRLRAFLRGFDGG
CCCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCC
FPTEYYAALNASEPLPDWSELFPARWADACKEAWQMELGQPGVFEVIANVLDRTREEHDG
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
NTILIGGPPCQAYSLAGRARNKGVADYVAEKDSRHFLYREYVEILKRLRPAVFVMENVKG
CEEEECCCCCHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MLSSKVNGGEIFERVLDDLRNAGDGYTLVPLSAQRNREHLAARDFIVRAEEHGVPQARHR
HHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHCE
VFVVGIRNDLPTPEGDMPLMPAVDQNCRADVHSVLGSLPALRSGLSRQRDNPAAWRKVVL
EEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EHAKRLIASKKVDSELRQYLAELKKSSLARAFERCQSTRSCDESGMPPQLAEWLIDPALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RISGHETRAHIAADLGRYLFVSAFGREYGRSPKLSEFPDYLLPAHRNRYSGAFADRFRVQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHH
IAERPSSTVTSHISKDGHYFIHPDPTQVRSLTVREAARLQTFPDNYVFCGNRTQQYHQVG
HHCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHC
NAVPPFLALRIARVIRDIMGRF
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]