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The map label for this gene is ddl

Identifier: 89055244

GI number: 89055244

Start: 2769670

End: 2770620

Strand: Reverse

Name: ddl

Synonym: Jann_2753

Alternate gene names: 89055244

Gene position: 2770620-2769670 (Counterclockwise)

Preceding gene: 89055245

Following gene: 89055243

Centisome position: 64.16

GC content: 63.72

Gene sequence:

>951_bases
GTGGCAGGGTTGAGCAGCCTTCCATCCCGTGTCGTCGTCTTGATGGGTGGCCCCTCCGCTGAGCGCGAGGTGTCGCTGAG
CACCGGGCGAGGGTGCGCCGAGGCGTTACGCGGTGAGGGATTTGACGTAATAGAAGTGGACCCGGGCGTGACCCACGCCG
GGGCGGAGCTGTGCGCACGTCTTCAAGAAATCCAGCCTGACGTTGTGTTCAATGCGCTCCACGGTCGTTGGGGGGAGGAC
GGATGCGTTCAGGGCCTTCTGGAATGGATGCGCCTTCCCTATACCCATTCCGGCGTTCTGTCCTCGGCCCTCGCCATGGA
CAAGACGCGGGCCAAGACTGCGCTGAAGGCCCATGGCCTGCCTGTCGTCGACAGCATTATTGCCCCGAAGGAGGCCGTCG
TCGCGGCCCATGTCATGGCCACACCCTACGTCGTGAAGCCCAACAATGAAGGCTCCTCCGTCGGCGTGTATCTGGTGAAC
GAAGCCGCCAATGGCCCGCCCCATCTGTCCGACGACATGCCCGATGAGGTGATGGTGGAGACCTACGCACCGGGCCGCGA
ATTGACGGTGTCGGTCCTGGGTGACGCGCCCGGCGATCCCGGCGCGCTGACTGTCACGGATATCCTGACCGACGGCTGGT
ACGATTATGATGCCAAGTACAAACCCGGCGGTTCCCGCCATGTGGTGCCCGCGCAGATCCCGCAGGAGATCTGGGACGCC
TGCATGGACATGGCCGTGCGCGCCCATACAATTCTGGGATGCAAGGGCGTCAGCCGGACCGACTATCGCTGGGATGAGGC
CCGCGGCCTTGACGGTTTGATCATCCTTGAAGTGAACACGCAGCCCGGGATGACGCCCACCTCCCTGACGCCCGAACAGG
GCGAAGCGGTTGGAATGTCCTTTGGCAAGTTGTGCCGCTGGTTGGTGGAGGACGCGACATGCGACCGTTGA

Upstream 100 bases:

>100_bases
AAGCTGCGCGACGACGAAAAACAAGCAAGGCTCTGCCAACGTGCAGGGGAAATCGATCCGGCAGGCAACGTATCAGCCGG
GCAATTTGAGGACATAAACG

Downstream 100 bases:

>100_bases
CTACCCGCAGCCCCGCGCGACCGTTGATTGCGCGCCGCTCCACCCCGGCGCCCACGCCCGCCCCTCACGATCCCGCGCCG
TCCAGGCTCAGCTACCGCGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAGLSSLPSRVVVLMGGPSAEREVSLSTGRGCAEALRGEGFDVIEVDPGVTHAGAELCARLQEIQPDVVFNALHGRWGED
GCVQGLLEWMRLPYTHSGVLSSALAMDKTRAKTALKAHGLPVVDSIIAPKEAVVAAHVMATPYVVKPNNEGSSVGVYLVN
EAANGPPHLSDDMPDEVMVETYAPGRELTVSVLGDAPGDPGALTVTDILTDGWYDYDAKYKPGGSRHVVPAQIPQEIWDA
CMDMAVRAHTILGCKGVSRTDYRWDEARGLDGLIILEVNTQPGMTPTSLTPEQGEAVGMSFGKLCRWLVEDATCDR

Sequences:

>Translated_316_residues
MAGLSSLPSRVVVLMGGPSAEREVSLSTGRGCAEALRGEGFDVIEVDPGVTHAGAELCARLQEIQPDVVFNALHGRWGED
GCVQGLLEWMRLPYTHSGVLSSALAMDKTRAKTALKAHGLPVVDSIIAPKEAVVAAHVMATPYVVKPNNEGSSVGVYLVN
EAANGPPHLSDDMPDEVMVETYAPGRELTVSVLGDAPGDPGALTVTDILTDGWYDYDAKYKPGGSRHVVPAQIPQEIWDA
CMDMAVRAHTILGCKGVSRTDYRWDEARGLDGLIILEVNTQPGMTPTSLTPEQGEAVGMSFGKLCRWLVEDATCDR
>Mature_315_residues
AGLSSLPSRVVVLMGGPSAEREVSLSTGRGCAEALRGEGFDVIEVDPGVTHAGAELCARLQEIQPDVVFNALHGRWGEDG
CVQGLLEWMRLPYTHSGVLSSALAMDKTRAKTALKAHGLPVVDSIIAPKEAVVAAHVMATPYVVKPNNEGSSVGVYLVNE
AANGPPHLSDDMPDEVMVETYAPGRELTVSVLGDAPGDPGALTVTDILTDGWYDYDAKYKPGGSRHVVPAQIPQEIWDAC
MDMAVRAHTILGCKGVSRTDYRWDEARGLDGLIILEVNTQPGMTPTSLTPEQGEAVGMSFGKLCRWLVEDATCDR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=319, Percent_Identity=37.3040752351097, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=33.6182336182336, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_JANSC (Q28NP2)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510695.1
- ProteinModelPortal:   Q28NP2
- SMR:   Q28NP2
- STRING:   Q28NP2
- GeneID:   3935217
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2753
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   TDGWYDY
- PhylomeDB:   Q28NP2
- ProtClustDB:   PRK01372
- BioCyc:   JSP290400:JANN_2753-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33707; Mature: 33575

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLSSLPSRVVVLMGGPSAEREVSLSTGRGCAEALRGEGFDVIEVDPGVTHAGAELCAR
CCCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHH
LQEIQPDVVFNALHGRWGEDGCVQGLLEWMRLPYTHSGVLSSALAMDKTRAKTALKAHGL
HHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PVVDSIIAPKEAVVAAHVMATPYVVKPNNEGSSVGVYLVNEAANGPPHLSDDMPDEVMVE
CHHHHHHCCHHHHHHHHHHHCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEE
TYAPGRELTVSVLGDAPGDPGALTVTDILTDGWYDYDAKYKPGGSRHVVPAQIPQEIWDA
ECCCCCEEEEEEECCCCCCCCCEEEEHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHH
CMDMAVRAHTILGCKGVSRTDYRWDEARGLDGLIILEVNTQPGMTPTSLTPEQGEAVGMS
HHHHHHHHHHEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
FGKLCRWLVEDATCDR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGLSSLPSRVVVLMGGPSAEREVSLSTGRGCAEALRGEGFDVIEVDPGVTHAGAELCAR
CCCCCCCCEEEEEECCCCCCCEEECCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHH
LQEIQPDVVFNALHGRWGEDGCVQGLLEWMRLPYTHSGVLSSALAMDKTRAKTALKAHGL
HHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PVVDSIIAPKEAVVAAHVMATPYVVKPNNEGSSVGVYLVNEAANGPPHLSDDMPDEVMVE
CHHHHHHCCHHHHHHHHHHHCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEE
TYAPGRELTVSVLGDAPGDPGALTVTDILTDGWYDYDAKYKPGGSRHVVPAQIPQEIWDA
ECCCCCEEEEEEECCCCCCCCCEEEEHHHHCCCCCCCCEECCCCCCCCCCHHHHHHHHHH
CMDMAVRAHTILGCKGVSRTDYRWDEARGLDGLIILEVNTQPGMTPTSLTPEQGEAVGMS
HHHHHHHHHHEEECCCCCCCCCCCHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
FGKLCRWLVEDATCDR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA