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The map label for this gene is nuoF2 [H]

Identifier: 89055050

GI number: 89055050

Start: 2560888

End: 2562390

Strand: Reverse

Name: nuoF2 [H]

Synonym: Jann_2559

Alternate gene names: 89055050

Gene position: 2562390-2560888 (Counterclockwise)

Preceding gene: 89055051

Following gene: 89055049

Centisome position: 59.34

GC content: 65.14

Gene sequence:

>1503_bases
ATGAGCATCAAGGTCTGGGTGCCCCGCGATGCGGCGGCAAAAGCCCTTGGGGCGGATGAGGTGGCTGCCGCGTTCAAAGC
GGCGGGGGCCGACGTTGTGCGCAATGGATCGCGCGGCATGATCTGGCTGGAGCCACTGGTGGAGGTCGAGCGGGACGGCG
TGCGCGTCGGATACGGCGCGGCGTCCGTGGGCGATGTGGCGGCGATCCTGGATGGATCAGCCGAGAGCCTCGGCCCCGTG
GACGAGATCCCGTTCTACGCCAGCCAGAACCGCCTGACCTTCCAGCGCTGCGGCGTGATCGATCCGCTGTCATTGGCGGA
GTACGAGGCCCACGGAGGGCTTACAGGTTTGCGTCGCGCGCTGGAGATGACGCCGGAGCAAATCACCACCCATGTCACCG
AGTCCGGCCTGCGCGGCCGAGGCGGCGCGGGCTTCCCGACCGGCATCAAGTGGAACACGGTGCGGCAGGCCGACGGGCCG
CAGAAGTACATCGTCTGCAATGCGGACGAGGGCGACTCGGGCACCTTCGCCGACCGCATGATCATGGAGGGCGATCCGTT
CTGCCTGATTGAGGGCATGGTGATCGCGGGCCTCGGCGTGGGGGCCACGAAGGGCTACATCTACCTGCGGTCCGAGTATC
CGGATGCGATTGAAGTGATGGAATCTGCCATTGAAATAACGCGTGAAAACAACGTCCTCGGGCACGTTCTTCATTCAGAA
CATGAGTTTGATATCGAGGTTCGTGTGGGGGCCGGTGCCTATGTCTGCGGCGAGGAAACCTCGCTCCTGAACAGTCTTGA
GGGCAAGCGCGGCGTGGTTCGCGCCAAGCCGCCTCTGCCCGCGCTGGAAGGCTTCCTGGGGCGGCCCACGGTGGTCAATA
ACGTGATCTCGCTGGCGACCGTTCCGGTGATCTTCGAGAAGGGCGCGGAGCATTATGCGAGCTTCGGGCTGGGTCGGTCC
AAAGGCACCATGCCGGTCCAGATCGCGGGCAACGTGAATCATGGTGGCCTGTTTGAGACGGCCTTCGGGATGCCTCTGGG
CGAGTTGGTGAATGATATCGCGGGCGGCAGCGCCACCGGTCGCCCCGTGAAGGCAGTGCAGGTTGGCGGGCCACTGGGCG
CTTATTTCGGGCCCGAGAAATTCGACACGCCGTTCGCCTATGAGCCCTTTGACGGGGCGGGCGGGTTGATCGGCCACGCG
GGCGTGGTGGTGTTTGACGACAGCGCGGACATGCTTGGCATGGCGCGCTTCGCGATGGAATTCTGCGCCATTGAGAGCTG
CGGGAAATGCACCCCCTGCCGCATCGGGGCGGTGCGCGGGGTTGAAACGCTCGACCGCATCCGCGAGGGCGACGCCACGG
CGATCCCGCTGCTGGTCGACCTGTGCGAGACAATGAAAGACGGATCGCTCTGCGCGCTGGGGGGCTTCACGCCGTATCCG
GTGATGAGCGCGCTGGAGCAGTTCCCTGAGGAGTTTGCGGGTGTTCGGGAGGCGGCGGAATGA

Upstream 100 bases:

>100_bases
GTGGCCCGGCGGCGATGATCGACGGCAAGCTGAAGGGTCGGGTGACCGCGGACAGCCTGATCGCAGAGGTGTCGCCTCAG
CAGGGCGCGGGAGCAATGTC

Downstream 100 bases:

>100_bases
CATTTGACCGGTTTTTCCCCGACTGCGTCGGCGACCAGCTGGGGGCCAGCCCCCAGACCCCCGGGATGTTTATGGAACGA
GGAAGAACAGGGAGTGTGGG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSIKVWVPRDAAAKALGADEVAAAFKAAGADVVRNGSRGMIWLEPLVEVERDGVRVGYGAASVGDVAAILDGSAESLGPV
DEIPFYASQNRLTFQRCGVIDPLSLAEYEAHGGLTGLRRALEMTPEQITTHVTESGLRGRGGAGFPTGIKWNTVRQADGP
QKYIVCNADEGDSGTFADRMIMEGDPFCLIEGMVIAGLGVGATKGYIYLRSEYPDAIEVMESAIEITRENNVLGHVLHSE
HEFDIEVRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPALEGFLGRPTVVNNVISLATVPVIFEKGAEHYASFGLGRS
KGTMPVQIAGNVNHGGLFETAFGMPLGELVNDIAGGSATGRPVKAVQVGGPLGAYFGPEKFDTPFAYEPFDGAGGLIGHA
GVVVFDDSADMLGMARFAMEFCAIESCGKCTPCRIGAVRGVETLDRIREGDATAIPLLVDLCETMKDGSLCALGGFTPYP
VMSALEQFPEEFAGVREAAE

Sequences:

>Translated_500_residues
MSIKVWVPRDAAAKALGADEVAAAFKAAGADVVRNGSRGMIWLEPLVEVERDGVRVGYGAASVGDVAAILDGSAESLGPV
DEIPFYASQNRLTFQRCGVIDPLSLAEYEAHGGLTGLRRALEMTPEQITTHVTESGLRGRGGAGFPTGIKWNTVRQADGP
QKYIVCNADEGDSGTFADRMIMEGDPFCLIEGMVIAGLGVGATKGYIYLRSEYPDAIEVMESAIEITRENNVLGHVLHSE
HEFDIEVRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPALEGFLGRPTVVNNVISLATVPVIFEKGAEHYASFGLGRS
KGTMPVQIAGNVNHGGLFETAFGMPLGELVNDIAGGSATGRPVKAVQVGGPLGAYFGPEKFDTPFAYEPFDGAGGLIGHA
GVVVFDDSADMLGMARFAMEFCAIESCGKCTPCRIGAVRGVETLDRIREGDATAIPLLVDLCETMKDGSLCALGGFTPYP
VMSALEQFPEEFAGVREAAE
>Mature_499_residues
SIKVWVPRDAAAKALGADEVAAAFKAAGADVVRNGSRGMIWLEPLVEVERDGVRVGYGAASVGDVAAILDGSAESLGPVD
EIPFYASQNRLTFQRCGVIDPLSLAEYEAHGGLTGLRRALEMTPEQITTHVTESGLRGRGGAGFPTGIKWNTVRQADGPQ
KYIVCNADEGDSGTFADRMIMEGDPFCLIEGMVIAGLGVGATKGYIYLRSEYPDAIEVMESAIEITRENNVLGHVLHSEH
EFDIEVRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPALEGFLGRPTVVNNVISLATVPVIFEKGAEHYASFGLGRSK
GTMPVQIAGNVNHGGLFETAFGMPLGELVNDIAGGSATGRPVKAVQVGGPLGAYFGPEKFDTPFAYEPFDGAGGLIGHAG
VVVFDDSADMLGMARFAMEFCAIESCGKCTPCRIGAVRGVETLDRIREGDATAIPLLVDLCETMKDGSLCALGGFTPYPV
MSALEQFPEEFAGVREAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=374, Percent_Identity=39.0374331550802, Blast_Score=239, Evalue=5e-63,
Organism=Homo sapiens, GI20149568, Length=374, Percent_Identity=39.0374331550802, Blast_Score=239, Evalue=5e-63,
Organism=Escherichia coli, GI1788620, Length=413, Percent_Identity=38.9830508474576, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17531727, Length=374, Percent_Identity=38.5026737967914, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI19920794, Length=373, Percent_Identity=37.8016085790885, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI45550452, Length=372, Percent_Identity=37.9032258064516, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24653819, Length=381, Percent_Identity=30.9711286089239, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24653817, Length=352, Percent_Identity=28.125, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 52700; Mature: 52569

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKVWVPRDAAAKALGADEVAAAFKAAGADVVRNGSRGMIWLEPLVEVERDGVRVGYGA
CEEEEECCCHHHHHHCCHHHHHHHHHHCCHHHHCCCCCCEEEECHHHHHCCCCEEEECCC
ASVGDVAAILDGSAESLGPVDEIPFYASQNRLTFQRCGVIDPLSLAEYEAHGGLTGLRRA
CCHHCCHHHHCCCCHHCCCHHHCCCEECCCCEEHHHCCCCCCCHHHHHHHCCCHHHHHHH
LEMTPEQITTHVTESGLRGRGGAGFPTGIKWNTVRQADGPQKYIVCNADEGDSGTFADRM
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCEECEEECCCCCCEEEEEECCCCCCCCHHHHE
IMEGDPFCLIEGMVIAGLGVGATKGYIYLRSEYPDAIEVMESAIEITRENNVLGHVLHSE
EECCCCEEEECCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCC
HEFDIEVRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPALEGFLGRPTVVNNVISLAT
CCEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHHCCCCCHHHHHHHHHHH
VPVIFEKGAEHYASFGLGRSKGTMPVQIAGNVNHGGLFETAFGMPLGELVNDIAGGSATG
HHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCC
RPVKAVQVGGPLGAYFGPEKFDTPFAYEPFDGAGGLIGHAGVVVFDDSADMLGMARFAME
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHH
FCAIESCGKCTPCRIGAVRGVETLDRIREGDATAIPLLVDLCETMKDGSLCALGGFTPYP
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHH
VMSALEQFPEEFAGVREAAE
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIKVWVPRDAAAKALGADEVAAAFKAAGADVVRNGSRGMIWLEPLVEVERDGVRVGYGA
EEEEECCCHHHHHHCCHHHHHHHHHHCCHHHHCCCCCCEEEECHHHHHCCCCEEEECCC
ASVGDVAAILDGSAESLGPVDEIPFYASQNRLTFQRCGVIDPLSLAEYEAHGGLTGLRRA
CCHHCCHHHHCCCCHHCCCHHHCCCEECCCCEEHHHCCCCCCCHHHHHHHCCCHHHHHHH
LEMTPEQITTHVTESGLRGRGGAGFPTGIKWNTVRQADGPQKYIVCNADEGDSGTFADRM
HHCCHHHHHHHHHHCCCCCCCCCCCCCCCEECEEECCCCCCEEEEEECCCCCCCCHHHHE
IMEGDPFCLIEGMVIAGLGVGATKGYIYLRSEYPDAIEVMESAIEITRENNVLGHVLHSE
EECCCCEEEECCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCC
HEFDIEVRVGAGAYVCGEETSLLNSLEGKRGVVRAKPPLPALEGFLGRPTVVNNVISLAT
CCEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHHCCCCCHHHHHHHHHHH
VPVIFEKGAEHYASFGLGRSKGTMPVQIAGNVNHGGLFETAFGMPLGELVNDIAGGSATG
HHHHHHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCCC
RPVKAVQVGGPLGAYFGPEKFDTPFAYEPFDGAGGLIGHAGVVVFDDSADMLGMARFAME
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCEEEECCCCHHHHHHHHHHH
FCAIESCGKCTPCRIGAVRGVETLDRIREGDATAIPLLVDLCETMKDGSLCALGGFTPYP
HHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCHHH
VMSALEQFPEEFAGVREAAE
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]