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The map label for this gene is purC

Identifier: 89055036

GI number: 89055036

Start: 2549022

End: 2549783

Strand: Reverse

Name: purC

Synonym: Jann_2545

Alternate gene names: 89055036

Gene position: 2549783-2549022 (Counterclockwise)

Preceding gene: 89055040

Following gene: 89055035

Centisome position: 59.05

GC content: 59.32

Gene sequence:

>762_bases
ATGGCACGCCGGAAGCTCGTCTATGAAGGCAAAGCAAAAACCCTGTATGAGGGGCCGGAGCCAGGCACGCTGGTGCAGTA
TTTCAAAGACGATGCCACCGCGGGCAATGGTGAGAAGCGCGACGTGATCGACGGCAAGGGCGTGTTGAACAACCGCCTGT
CGGAGTTCTTCATGAAGGGCCTTGGCAATATCGGGGTGCCGACGCATTTTATCAAACGCCTCAACATGCGCGAACAGCTG
ATCCGCGCCGTAGAGATCATTCCGCTGGAGGTTGTGGTGCGCAACACGGCGGCGGGCTCTATCTGTTCCCGTCTGGGGAT
CGAGGAGGGCACACCGATGCCGCGCCCGATCGTGGAATTCTACTTCAAGAATGACGACCTGAACGACCCGATTGTCAGTG
AAGAGCATATCATTGCCTTCGGGTGGGCCGCGCAGCAGGATTTGGACGATATGGTGAGCCTGGCCTTGCGGGTGAACGAT
TTCCTCAGCGGGCTGATGCTGGGGGTCGGCATCAAACTGGTGGATTTCAAGATCGAGATCGGGCGGATCTGGGATGGCGA
TTTCATGCGCCTGATCGTGGCCGATGAGATCAGCCCCGACAGTTGCCGCCTGTGGGATATCGAGACGGGGCGCAAGCTGG
ACAAAGACGTGTTCCGCCGCGATCTGGGGGACCTGGCCGATGCCTATACGGAAGTGGCCAAACGGCTCGGGATCATGCCC
CAGGGCCCGGTTGGCGCGATCAAGCCCACGCTGATTAACTGA

Upstream 100 bases:

>100_bases
CTCGGACGCGCGCGCTTGCGACACAACGCGCGATGACCTATAGCCCATCCCAATTGGCGGCAGCAGATCGGAGCCCGCCC
AAATTCCTGGGAATACGCGC

Downstream 100 bases:

>100_bases
TGCGCCCGCGCGGAATGGCCTGCGTCGGATTGAGTTGTATTTGCCAAGATGAAGGAGCGGGGCCATGAAAGCCGTTGTGA
CAGTGATGTTGAAGCAGGGC

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MARRKLVYEGKAKTLYEGPEPGTLVQYFKDDATAGNGEKRDVIDGKGVLNNRLSEFFMKGLGNIGVPTHFIKRLNMREQL
IRAVEIIPLEVVVRNTAAGSICSRLGIEEGTPMPRPIVEFYFKNDDLNDPIVSEEHIIAFGWAAQQDLDDMVSLALRVND
FLSGLMLGVGIKLVDFKIEIGRIWDGDFMRLIVADEISPDSCRLWDIETGRKLDKDVFRRDLGDLADAYTEVAKRLGIMP
QGPVGAIKPTLIN

Sequences:

>Translated_253_residues
MARRKLVYEGKAKTLYEGPEPGTLVQYFKDDATAGNGEKRDVIDGKGVLNNRLSEFFMKGLGNIGVPTHFIKRLNMREQL
IRAVEIIPLEVVVRNTAAGSICSRLGIEEGTPMPRPIVEFYFKNDDLNDPIVSEEHIIAFGWAAQQDLDDMVSLALRVND
FLSGLMLGVGIKLVDFKIEIGRIWDGDFMRLIVADEISPDSCRLWDIETGRKLDKDVFRRDLGDLADAYTEVAKRLGIMP
QGPVGAIKPTLIN
>Mature_252_residues
ARRKLVYEGKAKTLYEGPEPGTLVQYFKDDATAGNGEKRDVIDGKGVLNNRLSEFFMKGLGNIGVPTHFIKRLNMREQLI
RAVEIIPLEVVVRNTAAGSICSRLGIEEGTPMPRPIVEFYFKNDDLNDPIVSEEHIIAFGWAAQQDLDDMVSLALRVNDF
LSGLMLGVGIKLVDFKIEIGRIWDGDFMRLIVADEISPDSCRLWDIETGRKLDKDVFRRDLGDLADAYTEVAKRLGIMPQ
GPVGAIKPTLIN

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI5453539, Length=240, Percent_Identity=32.9166666666667, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI119220559, Length=247, Percent_Identity=31.5789473684211, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1788820, Length=238, Percent_Identity=42.436974789916, Blast_Score=204, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17531275, Length=225, Percent_Identity=29.7777777777778, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI18860083, Length=254, Percent_Identity=31.8897637795276, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24583917, Length=240, Percent_Identity=27.0833333333333, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_JANSC (Q28PA0)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_510487.1
- HSSP:   P0A7D7
- ProteinModelPortal:   Q28PA0
- SMR:   Q28PA0
- STRING:   Q28PA0
- GeneID:   3935008
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_2545
- eggNOG:   COG0152
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- PhylomeDB:   Q28PA0
- ProtClustDB:   PRK09362
- BioCyc:   JSP290400:JANN_2545-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 28265; Mature: 28134

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRKLVYEGKAKTLYEGPEPGTLVQYFKDDATAGNGEKRDVIDGKGVLNNRLSEFFMKG
CCCCCEEECCCCCEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LGNIGVPTHFIKRLNMREQLIRAVEIIPLEVVVRNTAAGSICSRLGIEEGTPMPRPIVEF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCCCHHHHHE
YFKNDDLNDPIVSEEHIIAFGWAAQQDLDDMVSLALRVNDFLSGLMLGVGIKLVDFKIEI
EECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
GRIWDGDFMRLIVADEISPDSCRLWDIETGRKLDKDVFRRDLGDLADAYTEVAKRLGIMP
EEEECCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QGPVGAIKPTLIN
CCCCCCCCCCCCC
>Mature Secondary Structure 
ARRKLVYEGKAKTLYEGPEPGTLVQYFKDDATAGNGEKRDVIDGKGVLNNRLSEFFMKG
CCCCEEECCCCCEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LGNIGVPTHFIKRLNMREQLIRAVEIIPLEVVVRNTAAGSICSRLGIEEGTPMPRPIVEF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHCCCCCCCCCCHHHHHE
YFKNDDLNDPIVSEEHIIAFGWAAQQDLDDMVSLALRVNDFLSGLMLGVGIKLVDFKIEI
EECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEE
GRIWDGDFMRLIVADEISPDSCRLWDIETGRKLDKDVFRRDLGDLADAYTEVAKRLGIMP
EEEECCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QGPVGAIKPTLIN
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA